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| 1 | Anti-HBc screening in Indian blood donors:Still an unresolved issue显示文摘AIM:To study the seroprevalence of antibody to hepatitis B core antigen (anti-HBc) in healthy blood donors negative for HBsAg and to evaluate whether anti-HBc detection could be adopted in India as a screening assay for HBV in addition to HBsAg. METHODS: A total of 1700 serum samples collected from HBsAg-negative healthy blood donors were tested for the presence of anti-HBc antibody (IgM + IgG). All samples reactive for anti-HBc antibody were then investigated for presence of anti-HBs and for liver function tests (LFTs). One hundred serum samples reactive for anti-HBc were tested for HBV DNA by PCR method. RESULTS: Out of 1700 samples tested, 142 (8.4%) blood samples were found to be reactive for anti-HBc. It was signif icantly lower in voluntary (6.9%) as compared to replacement donors (10.4%, P = 0.011). Seventy- two (50.7%) anti-HBc reactive samples were also reactive for anti-HBs with levels > 10 mIU/mL and 70 (49.3%) samples were non-reactive for anti-HBs, these units were labeled as anti-HBc-only. These 142 anti-HBc reactive units were also tested for liver function test. HBV DNA was detected in only 1 of 100 samples tested. CONCLUSION: Keeping in view that 8%-18% of donor population in India is anti-HBc reactive, inclusion of anti- HBc testing will lead to high discard rate. Anti-HBs as proposed previously does not seem to predict clearance of the virus. Cost effectiveness of introducing universalanti-HBc screening and discarding large number of blood units versus considering ID NAT (Individual donor nuclic acid testing) needs to be assessed. | Hari Krishan Dhawan Neelam Marwaha Ratti Ram Sharma Yogesh Chawla Beenu Thakral Karan Saluja Sanjeev Kumar Sharma Manish K Thakur Ashish Jain | 2008 | World Journal of Gastroenterology2008，14，34： | 8 |
| 2 | Ethanol steam reforming study over ZSM-5 supported cobalt versus nickel catalyst for renewable hydrogen generation显示文摘The renewable hydrogen generation through ethanol steam reforming is one of the anticipated areas for sustainable hydrogen generation. To elucidate the role of Ni and Co with ZSM-5 support, catalysts were prepared by wet impregnation method and ethanol steam reforming(ESR) was performed. The catalysts were characterized by HR-XRD, ATR–FTIR, HR-SEM, TEM with SAED, EDAX, surface area analyzer and TPR. It had shown complete ethanol conversion at 773 K, but the selectivity in hydrogen generation was found higher for 10% Ni/ZSM-5 catalyst as compared to 10% Co/ZSM-5. The 10% Ni/ZSM-5 catalyst has about 72% hydrogen selectivity at temperature 873 K. It indicates that Ni is a more sustainable catalyst as compared to Co with ZSM-5 support for ESR. The C_2H_4 was found major undesirable products up to 823 K temperature. Nevertheless, the 10% Ni/ZSM-5 catalyst had shown its stability for high temperature(873 K) ESR performance. | Ashutosh Kumar Ram Prasad Yogesh Chandra Sharma | 2019 | Chinese Journal of Chemical Engineering2019，27，3： | 2 |
| 3 | Genetic relationship among sugarcane traits under abiotic stresses 显示文摘 | Bakshi Ram Sahi B K Kumar S Sharma V P Cathurvedi B K | 2001 | Indian J Sugareane Technol2001，16，1： | 1 |
| 4 | Antioxidant activities of seabuckthorn ( Hippophae rhamnoides ) during hypoxia induced oxidative stress in glial cells显示文摘 | S. Narayanan D. Ruma B. Gitika S. K. Sharma T. Pauline M. Sai Ram G. Ilavazhagan R. C. Sawhney D. Kumar P. K. Banerjee | 2005 | Molecular and Cellular Biochemistry2005，，1： | 1 |
| 5 | Design and synthesis of 3, 5-diarylisoxazole derivatives as novel class of anti-hyperglycemic and lipid lowering agents显示文摘 | Atul Kumar Ram Awatar Maurya Siddharth Sharma | 2009 | Bioorganic & med- icinal chemistt32009，17，： | 1 |
| 6 | Thermomechanicalresponse of generalized thermoelastic diffusion with onerelaxation time due to time harmonic sources 显示文摘 | Ram P Sharma N Kumar R | 2008 | International Journal of Thermal Sciences2008，47，3： | 1 |
| 7 | Thermomechanical response of generalized thermoelastic diffusion with one relaxation time due to time harmonic sources显示文摘 | RAM P SHARMA N KUMAR R | 2008 | Inter-national Journal of Thermal Sciences2008，47，： | 1 |
| 8 | Protective efficacy of piperine against Mycobacterium tuberculosis显示文摘 | Sandeep Sharma Nitin Pal Kalia Pankaj Suden Prashant Singh Chauhan Manoj Kumar Anshu Beulah Ram Anamika Khajuria Sarang Bani Inshad Ali Khan | 2014 | Tuberculosis2014，，： | 1 |
| 9 | Reflection and transmission of micropolar elastic waves at an imperfect boundary显示文摘 | Kumar R Sharma N Ram P | 2008 | Multidiscipline Modelling in Materials and Structures2008，4，1： | 1 |
| 10 | Early Results of Core Decompression and Autologous Bone Marrow Mononuclear Cells Instillation in Femoral Head Osteonecrosis显示文摘 | Ramesh Kumar Sen Sujit Kumar Tripathy Sameer Aggarwal Neelam Marwaha Ratti Ram Sharma Niranjan Khandelwal | 2012 | The Journal of Arthroplasty2012，，5： | 1 |
| 11 | Antioxidant activities of seabuckthorn (Hippophae rhamnoides) during hypoxia induced oxidative stress in glial cells显示文摘 | S. Narayanan D. Ruma B. Gitika S. K. Sharma T. Pauline M. Sai Ram G. Ilavazhagan R. C. Sawhney D. Kumar P. K. Banerjee | 2005 | Molecular and Cellular Biochemistry (-)2005，，1： | 1 |
| 12 | Plant diversity of the Kangchenjunga Landscape, Eastern Himalayas显示文摘The Kangchenjunga Landscape (KL) in the Eastern Himalayas is a transboundary complex shared by Bhutan, India, and Nepal. It forms a part of the ‘Himalayan Biodiversity Hotspot’ and is one of the biologically richest landscapes in the Eastern Himalayas. In this paper, we use secondary information to review and consolidate the knowledge on the flora of the KL. We reviewed 215 journal articles, analysed the history of publications on the flora of the KL, their publication pattern in terms of temporal and spatial distribution and key research areas. Our review shows that the landscape has a long history of botanical research that dates back to the 1840s and progressed remarkably after the 1980s. Most of the studies have been carried out in India, followed by Nepal and Bhutan. The majority of these have been vegetation surveys, followed by research on ethnobotanical aspects and Non-Timber Forest Products (NTFPs). This paper describes the forest types and characteristic species of the KL and details the species richness, diversity and dominant families of seed plants. A total of 5198 species of seed plants belonging to 1548 genera and 216 families have been recorded from the landscape, including 3860 dicots, 1315 monocots and 23 gymnosperms. Among families, Orchidaceae is the most diversely represented family in terms of species richness. This paper also draws attention to the threatened and endemic flora of the KL, including 44 species that are threatened at national and global level and 182 species that are endemic. Finally, the paper reviews the major challenges facing the KL, the conservation efforts and practices that are currently in place and recommends systematic and comprehensive floral surveys, particularly long-term data collection and monitoring and transboundary collaboration, to address the existing knowledge gaps on floral diversity of the KL. | Pratikshya Kandel Nakul Chettri Ram P.Chaudhary Hemant Kumar Badola Kailash S.Gaira Sonam Wangchuk Namgay Bidha Yadav Uprety Eklabya Sharma | 2019 | Plant Diversity2019，41，3： | 1 |
| 13 | Design and synthesis of 3,5-diarylisoxazole derivatives as novel class of anti-hyperglycemic and lipid lowering agents显示文摘 | Atul Kumar Ram Awatar Maurya Siddharth Sharma Pervez Ahmad A.B. Singh A.K. Tamrakar Arvind K. Srivastava | 2009 | Bioorganic & Medicinal Chemistry2009，，14： | 1 |
| 14 | Thermomechanical response of generalized thermoelastic diffusion with one relaxation time due to time harmonic sources显示文摘 | RAM P SHARMA N KUMAR R | 2008 | Int J Thermal Sci2008，47，： | 1 |
| 15 | Wheat Stripe Rust Virulence and Varietal Resistance in the Foot Hill Himalayas of Nepal显示文摘 | Sarala Sharma Etienne Duveiller Chandra Bahadur Karki Dhruba Bahadur Thapa Ram Chandra Sharma Arun Kumar Joshi | 2015 | Journal of Agricultural Science and Technology(B)2015，5，7： | 0 |
| 16 | Underpinning the molecular programming attributing heat stress associated thermotoleranee in tea (Camellia sinensis (L.) O. Kuntze)显示文摘The most daunting issue of global climate change is the deleterious impact of extreme temperatures on tea productivity and quality,which has resulted in a quest among researchers and growers.The current study aims to unravel molecular programming underpinning thermotolerance by characterizing heat tolerance and sensitivity response in 20 tea cultivars.The signi fi cantly higher negative in fl uence of heat stress was recorded in a sensitive cultivar with reduced water retention(47%),chlorophyll content(33.79%),oxidation potential(32.48%),and increase in membrane damage(76.4%).Transcriptional pro fi ling of most tolerant and sensitive cultivars identi fi ed 78 differentially expressed unigenes with chaperon domains,including low and high molecular weight heat shock protein(HSP)and heat shock transcription factors(HSFs)involved in heat shock response(HSR).Further,predicted transcriptional interactome network revealed their key role in thermotolerance via well-co-ordinated transcriptional regulation of aquaporins,starch metabolism,chlorophyll biosynthesis,calcium,and ethylene mediated plant signaling system.The study identi fi ed the key role of HSPs(CsHSP90)in regulating HSR in tea,wherein,structure-based molecular docking revealed the inhibitory role of geldanamycin(GDA)on CsHSP90 by blocking ATP binding site at N-terminal domain of predicted structure.Subsequently,GDA mediated leaf disc inhibitor assay further af fi rmed enhanced HSR with higher expression of CsHSP17.6,CsHSP70,HSP101,and CsHSFA2 genes in tea.Through the current study,efforts were made to extrapolate a deeper understanding of chaperons mediated regulation of HSR attributing thermotolerance in tea. | Romit Seth Tony Kipkoech Maritim Rajni Parmar Ram Kumar Sharma | 2021 | Horticulture Research2021，8，1： | 0 |
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