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| 1 | 利用GBSSI和SBD作为“锚”将荧光素酶定位到淀粉粒中效果的比较显示文摘利用马铃薯淀粉粒结合型淀粉合成酶Ⅰ(GBSSI)作为“锚”,在马铃薯淀粉粒形成时将荧光素酶(LUC)定位到淀粉粒中.并对GBSSI/LUC重组蛋白质在转基因淀粉粒中的定位与积累、重组蛋白质中GBSSI与淀粉粒的亲和性以及荧光素酶的活性等进行了分析.结果表明,GBSSI/ LUC重组蛋白质在马铃薯淀粉粒中获得了特异性表达,但与以微生物的淀粉粒结合结构域(SBD) 作为“锚”的SBD/LUC重组蛋白质相比较,它与淀粉粒的亲和性以及荧光素酶的活性均下降.由此认为,在淀粉粒形成过程中,利用SBD作为“锚”将荧光素酶蛋白质定位到淀粉粒中的效果要优于GBSSI. | 季勤 Jean-Paul Vincken Krit Raemakers Luc C.J.M.Suurs Richard G.F.Visser | 2006 | 自然科学进展2006，16，6： | 4 |
| 2 | SBD在重组蛋白质中位移对其酶活性及与淀粉粒结合性能的影响显示文摘 将环状糊精糖基转移酶的SBD基因编码序列连接到萤光素酶基因的 5′ 端(SBD LUC)后,重组基因通过农杆菌介导导入马铃薯植株中.对重组蛋白在转基因淀粉粒中的定位与积累、重组蛋白中SBD与淀粉的亲和性以及萤光素酶的活性进行了分析比较.结果表明: 重组蛋白在马铃薯淀粉粒中获得了特异性表达, 当C 端SBD移位到重组萤光素酶蛋白的N 端后, SBD和淀粉的亲和性与LUC SBD重组蛋白质比较有所下降, 但萤光素酶活性则与马铃薯遗传背景有关. | 季勤 Jean-Paul Vincken Krit Raemakers Lucas Carolus Josephus Maria Suurs Richard Gerardus Franciscus Visser | 2005 | 南京师大学报(自然科学版)2005，28，1： | 3 |
| 3 | Effect of TDZ on plant regeneration from mature seeds in pea (Pisum sativum) 显示文摘 | ZHII-IUI S TZITZIKAS M RAEMAKERS K | 2009 | In Vitro Cellular & Developmental Biology-Plant2009，45，6： | 1 |
| 4 | Regeneration and transformation of cassava显示文摘 | RAEMAKERS C J J M SOFIARI E JACOBSEN E | 1997 | Euphytica1997，96，： | 1 |
| 5 | New concepts for re- lapsed Hodgkin' s disease显示文摘 | Josting A Raemakers JM Diehl V | 2002 | Ann Oncol2002，13，1： | 1 |
| 6 | Sec- ondary somatic embryogenesis and applications in plant breeding 显示文摘 | Raemakers C J J M Jacobsen E Visser R G F | 1995 | Euphytica1995，81，1： | 1 |
| 7 | Development and application of transgenic technologies in cassava显示文摘 | Taylor N Chavarriaga P Raemakers K | 2004 | Plant MolBiol2004，，56： | 1 |
| 8 | Secondary somatic embryogenesis and applications in plantbreeding显示文摘 | RAEMAKERS C J J M JACOBSEN E VISSER R G F | 1995 | Euphytica1995，81，： | 1 |
| 9 | A transformation method for obtaining marker - free plants of a cross - pollinating and vegetatively propagated crop 显示文摘 | de Vetten N Wolters A M Raemakers K | 2003 | Nature Biotech2003，21，： | 1 |
| 10 | Development and application of transgenic in cassava显示文摘 | Taylor N Chavarriaga P Raemakers K | 2004 | Plant Molecular Biology2004，56，4： | 1 |
| 11 | Effect of TDZ on plant regeneration from mature seeds in pea(Pisum sativum)显示文摘 | S Zhihui M Tzitzikas K Raemakers | 2009 | In Vitro Cellular&Developmental Biology-Plant2009，45，1： | 1 |
| 12 | Comparison of NAA and 2, 4-D induced somatic embryogenesis in cassava显示文摘 | Sofiari E Raemakers C J J M Kanju E et ol | 1997 | Tissue and Organ Culture1997，50，： | 1 |
| 13 | Secondary somatic embryogenesis and applications in plant breeding显示文摘 | Raemakers C J J M Jacobsen E Visser R G F | 1995 | Euphytica1995，81，： | 1 |
| 14 | Transgene Organisation in Potato After Particle Bombardment-mediated (co-)Transformation Using Plasmids and Gene Cassettes显示文摘 | Andrea Romano Krit Raemakers Jamila Bernardi Richard Visser Hans Mooibroek | 2003 | Transgenic Research2003，，4： | 1 |
| 15 | Improved cassava starch by antisense inhibition of granule-bound starch synthase I显示文摘 | RAEMAKERS K SCHREUDER M SUURS L | 2005 | Molecular Breeding2005，16，2： | 1 |
| 16 | Transgene Organisation in Potato After Particle Bombardment-mediated (co-)Transformation Using Plasmids and Gene Cassettes显示文摘 | Andrea Romano Krit Raemakers Jamila Bernardi Richard Visser Hans Mooibroek | 2003 | Transgenic Research2003，，4： | 1 |
| 17 | Secondary somatic embryogenesis and applications in plant breeding 显示文摘 | RAEMAKERS C J J M JACOBSEN E VISSER R G F | 1995 | Euphytica1995，81，1： | 1 |
| 18 | Cyclic somatic embryogenesis and plant regeneration in cassava 显示文摘 | Raemakers C J J M Amati M Staritsky G | 1993 | Ann Bot1993，71，： | 1 |
| 19 | Development of a plant regeneration system based on friable embryogenic callus in the ornamental Alstroemeria显示文摘 | H. -S. Lin C. van der Toorn K. J. J. M. Raemakers R. G. F. Visser M.J. De Jeu E. Jacobsen | 2000 | Plant Cell Reports2000，，5： | 1 |
| 20 | Transgene organization in potato after particle bombardmentmediated (co-)transformation using plasmids and gene cassettes显示文摘 | Romano A Raemakers K Bernardi J | | 0，，： | 1 |
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