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| 1 | Expression of Mitochondrial Gene Fragments within the Tapetum Induce Male Sterility by Limiting the Biogenesis of the Respiratory Machinery in Transgenic Tobacco显示文摘Plant mitochondrial genomes (mtDNAs) are large and undergo frequent recombination events. A common phenotype that emerges as a consequence of altered mtDNA structure is cytoplasmic-male sterility (CMS). The molecular basis for CMS remains unclear, but it seems logical that altered respiration activities would result in reduced pollen production. Analysis of tobacco (Nicotiana tabacum) mtDNAs indicated that CMS-associated loci often contain fragments of known organellar genes. These may assemble with organellar complexes and thereby interfere with normal respiratory functions. Here, we analyzed whether the expression of truncated fragments of mitochondrial genes (i.e. atp4, cox1 and rps3) may induce male sterility by limiting the biogenesis of the respiratory machinery. cDNA fragments corresponding to atp4f, cox1f and rps3f were cloned in-frame to a mitochondrial localization signal and a C-termini HA-tag under a tapetum-specific promoter and introduced to tobacco plants by Agrobacterium-mediated transformation. The constructs were then analyzed for their effect on mitochondrial activity and pollen fertility. Atp4f , Cox1f and Rps3f plants demonstrated male sterility phenotypes, which were tightly correlated with the expression of the recombinant fragments in the floral meristem. Fractionation of native organellar extracts showed that the recombinant ATP4f-HA, COX1f-HA and RPS3f-HA proteins are found in large membrane-associated particles. Analysis of the respiratory activities and protein profiles indicated that organellar complex I was altered in Atp4f, Cox1f and Rps3f plants. | Felix Shaya Svetlana Gaiduk Ido Keren Sofia Shevtsov Hanita Zemah Eduard Belausov Dalia Evenor Moshe Reuveni Oren Ostersetzer-Biran | 2012 | Journal of Integrative Plant Biology2012，54，2： | 4 |
| 2 | Nutrient supplemented serum-free medium increases cardiomyogenesis efficiency of human pluripotent stem cells显示文摘AIM: To development of an improved p38 MAPK inhibitor-based serum-free medium for embryoid body cardiomyocyte differentiation of human pluripotent stem cells. METHODS: Human embryonic stem cells (hESC) differentiated to cardiomyocytes (CM) using a p38 MAPK inhibitor (SB203580) based serum-free medium (SB media). Nutrient supplements known to increase cell viability were added to SB medium. The ability of these supplements to improve cardiomyogenesis was evaluated by measurements of cell viability, total cell count, and the expression of cardiac markers via flow cytometry. An improved medium containing Soy hydrolysate (HySoy) and bovine serum albumin (BSA) (SupSB media) was developed and tested on 2 additional cell lines (H1 and Siu-hiPSC). Characterization of the cardiomyocytes was done by immunohistochemistry, electrophysiology and quantitative real-time reverse transcriptionpolymerase chain reaction. RESULTS: hESC cell line, HES-3, differentiating in SB medium for 16 d resulted in a cardiomyocyte yield of 0.07 ± 0.03 CM/hESC. A new medium (SupSB media) was developed with the addition of HySoy and BSA to SB medium. This medium resulted in 2.6 fold increase in cardiomyocyte yield (0.21 ± 0.08 CM/hESC). The robustness of SupSB medium was further demonstrated using two additional pluripotent cell lines (H1, hESC and Siu1, hiPSC), showing a 15 and 9 fold increase in cardiomyocyte yield respectively. The age (passage number) of the pluripotent cells did not affect the cardiomyocyte yields. Embryoid body (EB) cardiomyocytes formed in SupSB medium expressed canonical cardiac markers (sarcomeric α-actinin, myosin heavy chain and troponin-T) and demonstrated all three major phenotypes: nodal-, atrial- and ventricular-like. Electrophysiological characteristics (maximum diastolic potentials and action potential durations) of cardiomyocytes derived from SB and SupSB media were similar. CONCLUSION: The nutrient supplementation (HySoy and BSA) leads to increase in cell viability, cell yield and cardiac marker expression during cardiomyocyte differentiation, translating to an overall increase in cardiomyocyte yield. | Sherwin Ting Marti Lecina Yau-Chi Chan Hung Fat Tse Shaul Reuveny Steve KW Oh | 2013 | World Journal of Stem Cells2013，5，3： | 4 |
| 3 | Polar - A potent polyoxin B compound for controlling powdery mildews in apple and nectarine trees, and grapevines 显示文摘 | Reuveni M Cohen H Zahavi T | 2000 | Crop Protect2000，19，6： | 2 |
| 4 | Drug exposure and psoriasis vulgaris: Case-control and case-crossover studies 显示文摘 | Cohen AD Bonneh DY Reuveni H | 2005 | Acta Derm Venereol2005，85，4： | 1 |
| 5 | Fusarium equiseti--a new cause of cumin spice plant wilt in Israel显示文摘 | Reuveni R | 1982 | Plant Disease1982，66，6： | 1 |
| 6 | Prognostic indicators for canine monocytic ehrlichiosis 显示文摘 | Shipov A Klement E Reuveni -Tager L | 2008 | Vet Parasito12008，153，12： | 1 |
| 7 | Controlling powdery mildew caused by Sphaerothecafuliginea in cucumber by foliar sprays of phosphate and potassium salts显示文摘 | Reuveni M Agapov V Reuveni R | 1996 | Crop Protection1996，15，1： | 1 |
| 8 | Foliar spray ophosphates induces growth increases and systemic resistance to Puccinia sorghi in maize(abstract)显示文摘 | REUVENI R GAPOV A V RUVENI M | 1994 | Plant Pathol1994，43，： | 1 |
| 9 | Modification of proton transport kinetics of the plasma membrane H^+-ATPase after adaptation of tobacco cells to NaCl 显示文摘 | Reuveni M Bressan R A Hasegawa P M | 1993 | J Plant Physiol1993，142，： | 1 |
| 10 | A foliar spray of micronutrient solutions induces local and systemic pro ection against powdery mildew(Sphaerotheca fuliginea) in cucumber plants显示文摘 | REUVENI R GAPOV A V RUVENI R | 1997 | Eur J Plant Pathol1997，103，： | 1 |
| 11 | Climate Change-induced Migration and Violent Conflict 显示文摘 | Reuveny R | 2007 | Political Geography2007，26，6： | 1 |
| 12 | Folia spray of phosphates induces growth increase and systemic resistance to Puccinia sorghi in maize显示文摘 | Reuveni R | 1994 | Plant Pathology1994，43，： | 1 |
| 13 | Folia spray of phosphates induces growth increase and systemic resistance to Puccinia sorghi in maize显示文摘 | Reuveni R Agapov V Reuveni M | 1994 | Plant Pathology1994，43，： | 1 |
| 14 | Efficacy of Trifloxystrobin(Flint),a New Strobilurin Fungicide,in Controlling Powdery Mildews on Apple,Mango and Nectarine,and Rust on Prune Trees显示文摘 | REUVENI M | 2000 | Crop Prot2000，19，5： | 1 |
| 15 | In vitro propagation of dioecious Carica papaya L显示文摘 | Reuveni O Shlesinger D R Laviu | 1990 | Plant Cell Tissue and Organ Culture1990，20，3： | 1 |
| 16 | Whitefly (Bemisia tabaci) genome project:analysis of sequenced clones from egg,instar,and adult (viruliferous and nonviruliferous) cDNA libraries显示文摘 | Leshkowitz D Gazit S Reuveni E Ghanim M Czosnek H McKenzie C Shatters Jr R L Brown J K | | 0，，： | 1 |
| 17 | Occurrence of metalaxyl-resistance isolates of Phytophthora infestans in potato fields in Israel显示文摘 | Y REUVENI M | 1983 | Phytopathology1983，，73： | 1 |
| 18 | Molecular dissection of tomato leaf curl virus resistance in tomato line TY172 derived from Solanum peruvianum显示文摘 | Anbinder I Reuveni M Azari R | 2009 | Theor Appl Genet2009，119，： | 1 |
| 19 | Drug exposure and psoriasis vulgaris:case-control and case-crossover studies显示文摘 | Cohen AD Bonneh DY Reuveni H | 2005 | Acta Derm Venereol2005，85，4： | 1 |
| 20 | Effects of azxoxystrobin,difenoconazole,polyoxin B(polar) and trifloxystrobin on gerurination and growth of Altemaria alternata and decay in red delicious apple fruit显示文摘 | Moshe Reuveni Dimitri Sheglov | | 0，，： | 1 |
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