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| 1 | 不同清洗方式对义齿白色假丝酵母菌生物膜清除效果的影响显示文摘目的比较不同清洁方式对义齿白色假丝酵母菌生物膜的清除能力。方法制作并消毒树脂片,在其表面培养白色假丝酵母菌生物膜,分别以化学(浸泡)、机械(刷洗)和复合(浸泡与刷洗并用)等方式处理覆有白色假丝酵母菌生物膜的树脂片,再将其浸入新鲜培养液进行残留生物膜的恢复培养,分别在0、6、24h后利用菌落计数进行效果评价。结果机械清除效果与牙膏种类密切相关,单纯的化学处理没有明显的清除作用,保丽净义齿清洁片复合处理清除白色假丝酵母菌生物膜的效果最佳,其处理后的树脂片培养24h仍未检出白色假丝酵母菌。结论不同的义齿清洗方法对附着在义齿表面的白色假丝酵母菌生物膜的清除能力有差异。其中,以保丽净义齿清洁片复合效果最好且能够持续地发挥抑菌作用。 | 李莉娜 Young Kim 舒毅 Renate Lux 田敏 Prasad Adusumilli Zvi Loewy 郭宏 Yu Shao 施文元 沈嵩 黄正蔚 田宇 朱昞 张恺 陈谦明 王敏 吴红崑 周学东 | 2010 | 国际口腔医学杂志2010，37，2： | 10 |
| 2 | Targeted Antimicrobial Therapy Against Streptococcus mutans Establishes Protective Non-cariogenic Oral Biofilms and Reduces Subsequent Infection显示文摘Aim Dental biofilms are complex communities composed largely of harmless bacteria. Certain pathogenic species including Streptococcus mutans (S. mutans) can become predominant when host factors such as dietary sucrose intake imbalance the biofilm ecology. Current approaches to control S. mutans infection are not pathogen-specific and eliminate the entire oral community along with any protective benefits provided. Here, we tested the hypothesis that removal of S. mutans from the oral community through targeted antimicrobial therapy achieves protection against subsequent S. mutans colonization. Methodology Controlled amounts of S. mutans were mixed with S. mutans-free saliva, grown into biofilms and visualized by antibody staining and cfu quantization. Two specifically-targeted antimicrobial peptides (STAMPs) against S. mutans were tested for their ability to reduce S. mutans biofilm incorporation upon treatment of the inocula. The resulting biofilms were also evaluated for their ability to resist subsequent exogenous S. mutans colonization. Results S. mutans colonization was considerably reduced (9 ± 0.4 fold reduction, P=0.01) when the surface was preoccupied with saliva-derived biofilms. Furthermore, treatment with S. mutans-specific STAMPs yielded S. mutans-deficient biofilms with significant protection against further S. mutans colonization (5 minutes treatment: 38 ± 13 fold reduction P=0.01; 16 hours treatment: 96 ± 28 fold reduction P=0.07). Conclusion S. mutans infection is reduced by the pre- sence of existing biofilms. Thus maintaining a healthy or 'normal' biofilm through targeted antimicrobial therapy (such as the STAMPs) could represent an effective strategy for the treatment and prevention of S. mutans colonization in the oral cavity and caries progression. | Li-na Li Li-hong Guo Renate Lux Randal Eckert Daniel Yarbrough Jian He Maxwell Anderson Wen-yuan Shi | 2010 | International Journal of Oral Science2010，2，2： | 5 |
| 3 | Development and evaluation of a safe and effective sugar-free herbal lollipop that kills cavity-causing bacteria显示文摘Dental caries (tooth decay) is caused by a specific group of cariogenic bacteria, like Streptococcus mutans, which convert dietary sugars into acids that dissolve the mineral in tooth structure. Killing cariogenic bacteria is an effective way to control or prevent tooth decay. In a previous study, we discovered a novel compound (Glycyrrhizol A), from the extraction of licorice roots, with strong antimicrobial activity against cariogenic bacteria. In the current study, we developed a method to produce these specific herbal extracts in large quantities, and then used these extracts to develop a sugar-free lollipop that effectively kills cariogenic bacteria like Streptococcus mutans. Further studies showed that these sugar-free lollipops are safe and their antimicrobial activity is stable. Two pilot human studies indicate that a brief application of these lollipops (twice a day for ten days) led to a marked reduction of cariogenic bacteria in oral cavity among most human subjects tested. This herbal lollipop could be a novel tool to promote oral health through functional foods. | Chu-hong Hu Jian He Randal Eckert Xiao-yang Wu Li-na Li Yan Tian Renate Lux Justin A. Shuffer Faina Gelman Janet Mentes Sue Spackman Janet Bauer Maxwell H. Anderson Wen-yuan Shi | 2011 | International Journal of Oral Science2011，3，1： | 3 |
| 4 | Characterization of Fusobacterium nucleatum ATCC 23726 adhesins involved in strain-specific attachment to Porphyromonas gingivalis显示文摘Bacterial adherence is an essential virulence factor in pathogenesis and infection. Fusobacterium nucleatum has a central role in oral bio?lm architecture by acting as a bridge between early Gram-positive and late Gram-negative colonizers that do not otherwise adhere to each other. In this study, we survey a key adherence interaction of F. nucleatum with Porphyromonas gingivalis, and present evidence that multiple fusobacterial adhesins have a role in the attachment of F. nucleatum ATCC 23726 to P. gingivalis in a highly strain-dependent manner. Interaction between these species displayed varying sensitivities to arginine,galactose and lactose. Arginine was found to hamper coaggregation by at least 62% and up to 89% with several P. gingivalis strains and galactose inhibition ranged from no inhibition up to 58% with the same P. gingivalis strains. Lactose consistently inhibited F. nucleatum interaction with these P. gingivalis strains ranging from 40% to 56% decrease in coaggregation. Among the adhesins involved are the previously described Fap2 and surprisingly, Rad D, which was described in an earlier study for its function in attachment of F. nucleatum to Gram-positive species. We also provide evidence for the presence of at least one additional adhesin that is sensitive to arginine but unlike Fap2 and Rad D, is not a member of the autotransporter family type of fusobacterial large outer membrane proteins. The strain-speci?c binding pro?le of multiple fusobacterial adhesins to P. gingivalis highlights the heterogeneity and complexity of interspecies interactions in the oral cavity. | Jane Park Bhumika Shokeen Susan K Haake Renate Lux | 2016 | International Journal of Oral Science2016，8，3： | 2 |
| 5 | In-vitro evidence for efficacy of antimicrobial mouthrinses显示文摘 | Pauline C. Pan Scott Harper Danette Ricci-Nittel Renate Lux Wenyuan Shi | 2010 | Journal of Dentistry2010，，： | 1 |
| 6 | Interspecies interactions within oral microbial communities显示文摘 | KURAMITSU H K HE Xue-song RENATE LUX | 2007 | Microbiol Molec Biol Rev2007，71，4： | 1 |
| 7 | Analysis of interspecies adherence of oral bacteria using a membrane binding assay coupled with polymerase chain reaction-denaturing gradient gel electrophoresis profiling显示文摘Information on co-adherence of different oral bacterial species is important for understanding interspecies interactions within oral microbial community. Current knowledge on this topic is heavily based on pariwise coaggregation of known, cultivable species. In this study, we employed a membrane binding assay coupled with polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) to systematically analyze the co-adherence profiles of oral bacterial species, and achieved a more profound knowledge beyond pairwise coaggregation. Two oral bacterial species were selected to serve as 'bait': Fusobacterium nucleatum (F. nucleatum) whose ability to adhere to a multitude of oral bacterial species has been extensively studied for pairwise interactions and Streptococcus mutans (S. mutans) whose interacting partners are largely unknown. To enable screening of interacting partner species within bacterial mixtures, cells of the 'bait' oral bacterium were immobilized on nitrocellulose membranes which were washed and blocked to prevent unspecific binding. The 'prey' bacterial mixtures (including known species or natural saliva samples) were added, unbound cells were washed off after the incubation period and the remaining cells were eluted using 0.2 mol·L-1 glycine. Genomic DNA was extracted, subjected to 16S rRNA PCR amplification and separation of the resulting PCR products by DGGE. Selected bands were recovered from the gel, sequenced and identified via Nucleotide BLAST searches against different databases. While few bacterial species bound to S. mutans, consistent with previous findings F. nucleatum adhered to a variety of bacterial species including uncultivable and uncharacterized ones. This new approach can more effectively analyze the co-adherence profiles of oral bacteria, and could facilitate the systematic study of interbacterial binding of oral microbial species. | Ren-ke Wang Xue-song He Wei Hu Renate Lux Ji-yao Li Xue-dong Zhou Wen-yuan Shi | 2011 | International Journal of Oral Science2011，3，2： | 1 |
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