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    题名 作者 年代 出处 被引量
1Exploiting multi-channels deep convolutional neural networks for multivariate time series classification显示文摘Yi ZHENG QiLIU Enhong CHEN Yong GE J. Leon ZHAO 2016Frontiers of Computer Science2016,10,1:20
2Expression of human augmenter of liver regeneration in pichia pastoris yeast and its bioactivity in vitro显示文摘AIM:To construct a yeast expression system of human augmenter of liver regeneration (hALR) and to examine its bioactivity in v/tro.METHODS: With PCR and gene recombination techniques, cDNA of open reading frame of hALR was obtained from recombinant plasmid pcDNA3.1-hALR and inserted into plasmid pPIC9. The cDNA of hALR from recombinant plasmid pPIC9-hALR demonstrated by sequencing was subcloned into plasmid pPIC9K. The recombinant plasmid pPIC9KhALR was transformed into GSl15 with electroporation. hALR was expressed by GSl15 under the induction of 5 mL/L methanol and purified with ultrafiltration after it was analyzed by 15% SDS-PAGE and Western blot. The effects of hALR on in vitro proliferation of QGY and HepG2 cells were evaluated by ^3H-TdR methods. RESULTS: The correctness and integrity of recombinant plasmids pPIC9-hALR and pPIC9K-hALR were identified by restriction digestion, PCR and sequencing methods, respectively, hALR as a secretive protein was successfully expressed by GSl15. Its molecular weight was about 15 ku and the target protein was about 60% of the total protein in the supernatant from GSl15 with plasmid pPIC9K-hALR. The results of Western blot of hALR showed the specific band. The high qualitative hALR was obtained through ultrafiltration, hALR could stimulate in vitro proliferation of QGY and HepG2 cells in a dose-dependent manner, but there was a difference in reactivity to hALR between 0GY and HepG2. CONCLUSION: The hALR as a secretive protein can be successfully expressed by GSl15. It may stimulate in vitro proliferation of QGY and HepG2 cells at a dose-dependent manner. But QGY and HepG2 cells have different reactivities to hALR.QiLiu Hui-FengYu HangSun Hua-FengMa 2004World Journal of Gastroenterology2004,10,21:14
3巯基乙酸浮选抑制黄铜矿的研究显示文摘通过黄铜矿样品的小批量浮选、红外光谱和吸附、解吸研究对有机抑制剂巯基乙酸进行了试验。结果表明,该药剂在矿表呈多层吸附并且强烈抑制黄铜矿,在充气溶液中这种作用更为强烈。依据研究结果,提出了巯基乙酸在矿物表面的吸附模型。G.W.Poling QiLiu 刘北林 1989国外金属矿选矿1989,,4:9
4The association of interleukin-16 gene polymorphisms with IL-16 serum levels and risk of nasopharyngeal carcinoma in a Chinese population显示文摘Xue Qin Qiliu Peng Xiaoxia Lao Zhiping Chen Yu Lu Xianjun Lao Cuiju Mo Jingzhe Sui Junrong Wu Limin Zhai Shi Yang Shan Li Jinmin Zhao 2014Tumor Biology2014,,3:2
5Generation of a triple‐gene knockout mammalian cell line using engineered zinc‐finger nucleases显示文摘Pei‐QiLiu Edmond M.Chan Gregory J.Cost LinZhang JianbinWang Jeffrey C.Miller Dmitry Y.Guschin AndreasReik Michael C.Holmes John E.Mott Trevor N.Collingwood Philip D.Gregory 2010Biotechnol Bioeng2010,,1:1
6ERCC1 expression levels predict the outcome of platinum-based chemotherapies in advanced bladder cancer: a meta-analysis显示文摘Shan Li Junrong Wu Yongbin Chen Weizhong Tang Qiliu Peng Yan Deng Li Xie Jian Wang Shan Huang Ruolin Li Xue Qin Jinmin Zhao 2014Anti-Cancer Drugs2014,,:1
7Robust Hollow Spheres Consisting of Alternating Titania Nanosheets and Graphene Nanosheets with High Photocatalytic Activity for CO<sub>2</sub> Conversion into Renewable Fuels显示文摘WenguangTu YongZhou QiLiu ZhongpingTian JunGao XiaoyuChen HaitaoZhang JianguoLiu ZhigangZou 2012Adv Funct Mater2012,,6:1
8Association of IL-2 polymorphisms and IL-2 serum levels with susceptibility to HBV-related hepatocellular carcinoma in a Chinese Zhuang population显示文摘Qiliu Peng Haiwei Li Xianjun Lao Yan Deng Zhiping Chen Xue Qin Shan Li 2014Infection, Genetics and Evolution2014,,:1
9Association of IL27 gene polymorphisms and HBV-related hepatocellular carcinoma risk in a Chinese population显示文摘Qiliu Peng Xue Qin Yani He Zhiping Chen Yan Deng Taijie Li Li Xie Jinmin Zhao Shan Li 2013Infection, Genetics and Evolution2013,,:1
10Recurrent nasopharyngeal Carcinoma and post radiation fibrosis: differentiation with enhanced MRI显示文摘Magnetic resonance imaging (MRI) was performed in 40 patients with nasopharyngeal carcinoma (NPC) before and after i.v. administration of Cadolinium-DTPA(Gd-DTPA), for evaluating the role of Gd-DTPA-enhanced imaging in the differentiation of recurrent nasopharyngeal carcinoma (PNPC) from post-radiation fibrosis (RF). MRI diagnosis and findings were confirmedby pathology (n=16) and long-term serial MR/CT and clinical follow-up (n=24), including 3 untreated primary NPC cases,20RNPC cases and 17 RF cases. The results indicated that post-contrast T1 weighted imaging exhibited excellent contrast between thelesion and the surrounding tissue, therefore, it was superior to the Pre-conbet T1WI in showing the relationship between lesionsand adjacent tissues, especially for those lesions located submucosally, and the ones with intl’aCndal invasion. All the NPC andRNPC demonstrated mild to moderate enhancement, with the pattern of homogenous masses or patches. Only 5 cases of the RFgroup demonstrated mild enhancement, which was found in 4 of the 5 cases within one year after radiotherapy. Of the 5 cases,4revealed linear-shaped enhancement. The mean value of enhancement percentage showed marked difference between the twogroups (P<0.05), however, some overlapping existed in their mean ± SD. Gd-DTPA enhanced MR imaging could offer importantinformation for differentiating from RF, on the basis of the intensity and pattern characteristics of the enhancement. It couldalso be helpful in separating thickened mucosa or sinus secretion from tumor, and was superior to unenhanced MR imaging in theearly diagnosis of intracranial involvement of NPC.Chen Yanping Huang Qiliu (Imaging Dagnostic Centre, Nanfang Hospital, First Military Medical University, Guangzhou, 510515) 1998Journal of Medical Colleges of PLA(China)1998,13,1:0
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