|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Epigenetic and non-epigenetic mode of SIRT1 action during oocyte meiosis progression显示文摘Background: SIRT1 histone deacetylase acts on many epigenetic and non-epigenetic targets. It is thought that SIRT1 is involved in oocyte maturation;therefore, the importance of the ooplasmic SIRT1 pool for the further fate of mature oocytes has been strongly suggested. We hypothesised that SIRT1 plays the role of a signalling molecule in mature oocytes through selected epigenetic and non-epigenetic regulation.Results: We observed SIRT1 re-localisation in mature oocytes and its association with spindle microtubules.In mature oocytes, SIRT1 distribution shows a spindle-like pattern, and spindle-specific SIRT1 action decreasesα-tubulin acetylation. Based on the observation of the histone code in immature and mature oocytes, we suggest that SIRT1 is mostly predestined for an epigenetic mode of action in the germinal vesicles(GVs) of immature oocytes. Accordingly, BML-278-driven trimethylation of lysine K9 in histone H3 in mature oocytes is considered to be a result of GV epigenetic transformation.Conclusions: Taken together, our observations point out the dual spatiotemporal SIRT1 action in oocytes,which can be readily switched from the epigenetic to non-epigenetic mode of action depending on the progress of meiosis. | Jan Nevoral Lukas Landsmann Miriam Stiavnicka Petr Hosek Jiri Moravec Sarka Prokesova Hedvika Rimnacova Eliska Koutna Pavel Klein Kristyna Hoskova Tereza Zalmanova Tereza Fenclova Jaroslav Petr Milena Kralickova | 2019 | Journal of Animal Science and Biotechnology2019，10，4： | 3 |
| 2 | Preparation of nanosized micro/mesoporous composites via simultaneous synthesis of Beta/MCM-48phases显示文摘 | Prokesova P Mintova S Cejka J | | 0，，1： | 1 |
| 3 | Preparation of nanosized micro/mesoporous composites显示文摘 | Prokesova P Mintova S Cejka J | 2003 | Materials Science and Engineering2003，23，68： | 1 |
| 4 | Preparation of nanosized micro/mesoporous composites via simultaneous synthesis of Beta/MCM-48 phases显示文摘 | Prokesova P Mintova S Cejka J | 2003 | Microporous and Mesoporous Materials2003，64，13： | 1 |
| 5 | Catalytic activity of micro/mesoporous composites in toluene alkylation with propylene显示文摘 | Prokesova Nadezda Zilkova | 2004 | Applied Catalysis A:General2004，281，2005： | 1 |
| 6 | Preparation of nanosized- micro/mesoporous composites via simultaneous synthesis of Beta/ MCM-48 phases 显示文摘 | PROKESOVA P MINTOVA S BEIN T | 2003 | Microporous Mesoporous Mater2003，64，： | 1 |
| 7 | Preparation of nanosized micro/mesoporous composites via simultaneous synthesis of BetaJMCM-48 phases显示文摘 | Prokesova P Mintova S Cejka J | 2003 | Microporousand Mesoporous Materials2003，64，13： | 1 |
| 8 | Preparation of Nanosized Micro/Mesoporous Composites Via Simultaneous Synthesis of Beta/MCM-48 Phases显示文摘 | Prokesova P Mintova S Bein T | 2003 | Microporous Mesoporous Mater2003，64，： | 1 |
| 9 | Catalytic activity of mlcro/mesoporous composites in toluene alkylation w/th propylene显示文摘 | Prokesova P Zilkova N Mintova S | 2005 | Appl Catal A2005，281，12： | 1 |
| 10 | lmmu- nostimulatory effect of Bacillus firmus on mouse lymphocytes 显示文摘 | PROKESOVA L MLCKOVA P STANKOVA I | 2002 | Folia Microbiol2002，47，2： | 1 |
| 11 | Preparation of nanosized micro/mesoporous composites via simultaneous synthesis of Beta/MCM-48 phases显示文摘 | Prokesova P Mintova S Cejka J C | 2003 | Microporous and Mesoporous Materials2003，64，： | 1 |
| 12 | Impaired function of regulatory T cells in cord blood of children of allergic moth- ers显示文摘 | Hrdy J Kocourkova I Prokesova L | 2012 | Clin Exp Immunol2012，170，1： | 1 |
| 13 | Preparation of nanosized micro/mesoporous composites via simultaneous synthesis of beta/MCM-48 phases 显示文摘 | PROKESOVA P MINTOVA S CEJKA J | 2003 | Microporous Mesoporous Mater2003，64，13： | 1 |
| 14 | Immunostimula- tory effect of bacillus firmus on mouse lymphocytes显示文摘 | Prokesova L Mlckova P Stankova I | 2002 | Fo- lia Microbiol2002，47，： | 1 |
| 15 | Non-invasive prediction of persistent villous atrophy in celiac disease显示文摘BACKGROUND Celiac disease(CD)is an immune-mediated enteropathy that is primarily treated with a gluten-free diet(GFD).Mucosal healing is the main target of the therapy.Currently,duodenal biopsy is the only way to evaluate mucosal healing,and noninvasive markers are challenging.Persistent elevation of anti-tissue transglutaminase antibodies(aTTG)is not an ideal predictor of persistent villous atrophy(VA).Data regarding prediction of atrophy using anti-deamidated gliadin peptide antibodies(aDGP)and abdominal ultrasonography are lacking.AIM To evaluate the ability of aTTG,aDGP,small bowel ultrasonography,and clinical and laboratory parameters in predicting persistent VA determined using histology.METHODS Patients with CD at least 1 year on a GFD and available follow-up duodenal biopsy,levels of aTTG and aDGP,and underwent small bowel ultrasonography were included in this retrospective cohort study.We evaluated the sensitivity,specificity,and positive and negative predictive values of aTTG,aDGP,small bowel ultrasonography,laboratory and clinical parameters to predict persistent VA.A receiver operating characteristic(ROC)curve analysis of antibody levels was used to calculate cut off values with the highest accuracy for atrophy prediction.RESULTS Complete data were available for 82 patients who were followed up over a period of four years(2014-2018).Among patients included in the analysis,women(67,81.7%)were predominant and the mean age at diagnosis was 33.8 years.Followup biopsy revealed persistent VA in 19 patients(23.2%).The sensitivity and specificity of aTTG using the manufacturer’s diagnostic cutoff value to predict atrophy was 50%and 85.7%,respectively,while the sensitivity and specificity of aDGP(using the diagnostic cutoff value)was 77.8%and 75%,respectively.Calculation of an optimal cutoff value using ROC analysis(13.4 U/mL for aTTG IgA and 22.6 U/mL for aDGP IgA)increased the accuracy and reached 72.2%[95%confidence interval(CI):46.5-90.3]sensitivity and 90%(95%CI:79.5-96.2)specificity for aDGP IgA and 66.7%(95%CI:41.0-86.7)sensitivity and 93.7%(95%CI:84.5-98.2)specificity for aTTG IgA.The sensitivity and specificity of small bowel ultrasonography was 64.7%and 73.5%,respectively.A combination of serology with ultrasound imaging to predict persistent atrophy increased the positive predictive value and specificity to 88.9%and 98%for aTTG IgA and to 90.0%and 97.8%for aDGP IgA.Laboratory and clinical parameters had poor predictive values.CONCLUSION The sensitivity,specificity,and negative predictive value of aTTG and aDGP for predicting persistent VA improved by calculating the best cutoff values.The combination of serology and experienced bowel ultrasound examination may achieve better accuracy for the detection of atrophy. | Barbora Packova Petra Kovalcikova Zdenek Pavlovsky Daniel Bartusek Jitka Prokesova Jiri Dolina Radek Kroupa | 2020 | World Journal of Gastroenterology2020，26，26： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员