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| 1 | Surveillance of multidrug resistant uropathogenic bacteria in hospitalized patients in Indian显示文摘Objective:To record surveillance,antibiotic resistance of uropathogens of hospitalized patients over a period of 18 months.Methods:Urine samples from wards and cabins were used for isolating urinary tract infection(UTI)-causing bacteria that were cultured on suitable selective media and identified by biochemical tests;and their antibiograms were ascertained by Kirby-Bauer's disc diffusion method,in each 6-month interval of the study period,using 18 antibiotics of five different classes.Results:From wards and cabins,1 245 samples were collected,from which 996 strains of bacteria belonging to 11 species were isolated,during April 2011 to September2012.Two Gram-positive,Staphylococcus aureus(S.aureus)and Enterococcus faecalis(E.faecalis),and nine Gram-negative bacteria,Acinetobacter baumannii,Citrobactcr sp.,Escherichia coli,Enterobacter aerogenes,Klebsiella pneumoniae.Klebsiella oxytoca,Proteus mirabilis,Proteus vulgaris and Pseudomonas aeruginosa were isolated.Both S.aureus and E.faecalis were vancomycin resistant,and resistant-strains of all pathogens increased in each 6-month period of study.Particularly,all Gram-negatives were resistant to nitrofurantoin and co-trimoxazole,the most preferred antibiotics of empiric therapy for UTI.Conclusions:Antibiograms of 11 UTI-causing bacteria recorded in this study indicated moderately higher numbers of strains resistant to each antibiotic studied,generating the fear of precipitating fervent episodes in public health particularly with bacteria,Acinetobacter baumannii,Escherichia coli,Klebsiella pneumoniae and S.aureus.Moreover,vancomycin resistance in strains of S.aureus and E.faecalis is a matter of concern. | Monali Priyadarsini Mishra Nagen Kumar Debata Rabindra Nath Padhy | 2013 | Asian Pacific Journal of Tropical Biomedicine2013，3，4： | 6 |
| 2 | Clonal isolation of endothelial colony-forming cells from early gestation chorionic villi of human placenta for fetal tissue regeneration显示文摘BACKGROUND Endothelial colony-forming cells(ECFCs)have been implicated in the process of vascularization,which includes vasculogenesis and angiogenesis.Vasculogenesis is a de novo formation of blood vessels,and is an essential physiological process that occurs during embryonic development and tissue regeneration.Angiogenesis is the growth of new capillaries from pre-existing blood vessels,which is observed both prenatally and postnatally.The placenta is an organ composed of a variety of fetal-derived cells,including ECFCs,and therefore has significant potential as a source of fetal ECFCs for tissue engineering.AIM To investigate the possibility of isolating clonal ECFCs from human early gestation chorionic villi(CV-ECFCs)of the placenta,and assess their potential for tissue engineering.METHODS The early gestation chorionic villus tissue was dissociated by enzyme digestion.Cells expressing CD31 were selected using magnetic-activated cell sorting,and plated in endothelial-specific growth medium.After 2-3 wks in culture,colonies displaying cobblestone-like morphology were manually picked using cloning cylinders.We characterized CV-ECFCs by flow cytometry,immunophenotyping,tube formation assay,and Dil-Ac-LDL uptake assay.Viral transduction of CVECFCs was performed using a Luciferase/tdTomato-containing lentiviral vector,and transduction efficiency was tested by fluorescent microscopy and flow cytometry.Compatibility of CV-ECFCs with a delivery vehicle was determined using an FDA approved,small intestinal submucosa extracellular matrix scaffold.RESULTS After four passages in 6-8 wks of culture,we obtained a total number of 1.8×107 CV-ECFCs using 100 mg of early gestational chorionic villus tissue.Immunophenotypic analyses by flow cytometry demonstrated that CV-ECFCs highly expressed the endothelial markers CD31,CD144,CD146,CD105,CD309,only partially expressed CD34,and did not express CD45 and CD90.CV-ECFCs were capable of acetylated low-density lipoprotein uptake and tube formation,similar to cord blood-derived ECFCs(CB-ECFCs).CV-ECFCs can be transduced with a Luciferase/tdTomato-containing lentiviral vector at a transduction efficiency of 85.1%.Seeding CV-ECFCs on a small intestinal submucosa extracellular matrix scaffold confirmed that CV-ECFCs were compatible with the biomaterial scaffold.CONCLUSION In summary,we established a magnetic sorting-assisted clonal isolation approach to derive CV-ECFCs.A substantial number of CV-ECFCs can be obtained within a short time frame,representing a promising novel source of ECFCs for fetal treatments. | Kewa Gao Siqi He Priyadarsini Kumar Diana Farmer Jianda Zhou Aijun Wang | 2020 | World Journal of Stem Cells2020，12，2： | 2 |
| 3 | Bioactive extracellular matrix scaffolds engineered with proangiogenic proteoglycan mimetics and loaded with endothelial progenitor cells promote neovascularization and diabetic wound healing显示文摘Diabetic ischemic wound treatment remains a critical clinical challenge.Neovascularization plays a significant role in wound healing during all stages of the tissue repair process.Strategies that enhance angiogenesis and neovascularization and improve ischemic pathology may promote the healing of poor wounds,particularly diabetic wounds in highly ischemic conditions.We previously identified a cyclic peptide LXW7 that specifically binds to integrinαvβ3 on endothelial progenitor cells(EPCs)and endothelial cells(ECs),activates vascular endothelial growth factor(VEGF)receptors,and promotes EC growth and maturation.In this study,we designed and synthesized a multi-functional pro-angiogenic molecule by grafting LXW7 and collagen-binding peptides(SILY)to a dermatan sulfate(DS)glycosaminoglycan backbone,named LXW7-DS-SILY,and further employed this multi-functional molecule to functionalize collagen-based extracellular matrix(ECM)scaffolds.We confirmed that LXW7-DS-SILY modification significantly promoted EPC attachment and growth on the ECM scaffolds in vitro and supported EPC survival in vivo in the ischemic environment.When applied in an established Zucker Diabetic Fatty(ZDF)rat ischemic skin flap model,LXW7-DS-SILY-functionalized ECM scaffolds loaded with EPCs significantly improved wound healing,enhanced neovascularization and modulated collagen fibrillogenesis in the ischemic environment.Altogether,this study provides a promising novel treatment to accelerate diabetic ischemic wound healing,thereby reducing limb amputation and mortality of diabetic patients. | Siqi He Tanaya Walimbe Hongyuan Chen Kewa Gao Priyadarsini Kumar Yifan Wei Dake Hao Ruiwu Liu Diana L.Farmer Kit S.Lam Jianda Zhou Alyssa Panitch Aijun Wang | 2022 | Bioactive Materials2022，7，4： | 2 |
| 4 | Role of phenolic O-H and methylene hydrogen on the free radical reactions and antioxidant activity of curcumin显示文摘 | K.Indira Priyadarsini Dilip K. Maity G.H. Naik M.Sudheer Kumar M.K. Unnikrishnan J.G. Satav Hari Mohan | 2003 | Free Radical Biology and Medicine2003，，： | 2 |
| 5 | Intrinsic apoptosis and NF-κB signaling are potential molecular targets for chemoprevention by black tea polyphenols in HepG2 cells in vitro and in a rat hepatocarcinogenesis model in vivo显示文摘 | R. Senthil Murugan R. Vidya Priyadarsini K. Ramalingam Y. Hara D. Karunagaran S. Nagini | 2010 | Food and Chemical Toxicology2010，，11： | 2 |
| 6 | Exosomal microRNAs from mesenchymal stem/stromal cells: Biology and applications in neuroprotection显示文摘Mesenchymal stem/stromal cells(MSCs)are extensively studied as cell-therapy agents for neurological diseases.Recent studies consider exosomes secreted by MSCs as important mediators for MSCs’neuroprotective functions.Exosomes transfer functional molecules including proteins,lipids,metabolites,DNAs,and coding and non-coding RNAs from MSCs to their target cells.Emerging evidence shows that exosomal microRNAs(miRNAs)play a key role in the neuroprotective properties of these exosomes by targeting several genes and regulating various biological processes.Multiple exosomal miRNAs have been identified to have neuroprotective effects by promoting neurogenesis,neurite remodeling and survival,and neuroplasticity.Thus,exosomal miRNAs have significant therapeutic potential for neurological disorders such as stroke,traumatic brain injury,and neuroinflammatory or neurodegenerative diseases and disorders.This review discusses the neuroprotective effects of selected miRNAs(miR-21,miR-17-92,miR-133,miR-138,miR-124,miR-30,miR146a,and miR-29b)and explores their mechanisms of action and applications for the treatment of various neurological disease and disorders.It also provides an overview of state-of-the-art bioengineering approaches for isolating exosomes,optimizing their yield and manipulating the miRNA content of their cargo to improve their therapeutic potential. | Aida Nasirishargh Priyadarsini Kumar Lalithasri Ramasubramanian Kaitlin Clark Dake Hao Sabrina V Lazar Aijun Wang | 2021 | World Journal of Stem Cells2021，13，7： | 2 |
| 7 | Free radical scavenging ability and antioxidant efficiency of curcumin and its substituted analogue显示文摘 | Khopde SM Priyadarsini KI Venkatesan P | 1999 | Biophys Chem1999，80，1： | 1 |
| 8 | Building energy efficiency labeling programme in Singapore显示文摘 | Siew Eang Lee Priyadarsini Rajagopalan | 2008 | Energy Policy2008，，10： | 1 |
| 9 | Rutile filled PTFE composites for flexible microwave substrate applications显示文摘 | RAJESH S MURALI K P PRIYADARSINI V | 2009 | Materials Science and Engineering B2009，163，1： | 1 |
| 10 | Comparative antioxidant activity of individual herbal components used in Ayurvedic medicine 显示文摘 | Naik G H Priyadarsini K I Satav J G | 2003 | Phytochem Anal2003，63，1： | 1 |
| 11 | Ellagie acid coordinately attenuates Wnt/β-eatenin and NF-KB signaling pathways to induce intrinsic apoptosis in an animal model of oral oncogenesis 显示文摘 | Anitha P Priyadarsini R V Kavitha K | 2013 | Eur J Nutr2013，52，1： | 1 |
| 12 | Comparative antioxi- dant activity of individual herbal components used in Ayurvedie medicine显示文摘 | Naik G H Priyadarsini K I Satav J G | 2003 | Phytochemistry2003，63，1： | 1 |
| 13 | Antioxidant and anti- proliferative activity of curcumin semicarbazone 显示文摘 | Dutta S Padhie S Priyadarsini KI | 2005 | Bioorg Med Chem Lett2005，15，11： | 1 |
| 14 | Role of phenolic O-H and methylene hydrogen on the free radical reactions and antioxi- dant activity of curcumin 显示文摘 | Priyadarsini KI Malty DK Naik GH | 2003 | Free Radic Biol Med2003，35，5： | 1 |
| 15 | Anticlastogenic activity of morin against whole body gamma irradiation in Swiss albino mice显示文摘 | Vipan Kumar Parihar Koiram Rajanna Prabhakar Veeresh Prabhakar Veerapur Kavirayani Indira Priyadarsini Mazhuvancherry Kesavan Unnikrishnan Chamallamudi Mallikajuna Rao | 2006 | European Journal of Pharmacology2006，，1： | 1 |
| 16 | Ameliorative effects of curcumin against renal injuries mediated by inducible nitric oxide synthase and nuclear factor kappa B during gentamicin-induced toxicity in Wistar rats显示文摘 | Ramar Manikandan Manikandan Beulaja Raman Thiagarajan Asokan Priyadarsini Rajendran Saravanan Munusamy Arumugam | 2011 | European Journal of Pharmacology2011，，2： | 1 |
| 17 | Role of phenolic O-H and methylene hydrogen on the free radical reactions and antioxidant activity of curcumin显示文摘 | PRIYADARSINI K I MAITY D K NAIK C H | 2003 | Free Radical Biology & Medicine2003，35，5： | 1 |
| 18 | Role of phenolic O-H and methylene hydrogen on the free radical reactions and antioxidant activity of curcumin显示文摘 | Priyadarsini KI Malty DK Naik GH | | 0，，05： | 1 |
| 19 | Inhibition of peroxynitrite- mediated reactions by vanillin 显示文摘 | Kumar SS Priyadarsini KI Sainis KB | 2004 | J Agr Food Chem2004，52，1： | 1 |
| 20 | The flavonoid quercetin induces cell cycle arrest and mitochondria-mediated apoptosis in human cervical cancer (HeLa) cells through p53 induction and NF-KB inhibition 显示文摘 | Vidya Priyadarsini R Senthil Murugan R Maitreyi S | 2010 | Eur J Pharmacol2010，649，13： | 1 |
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