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| 1 | 间充质的干细胞的隔离和描述源于人的胎盘组织显示文摘 AIM: To explore the feasibility of placenta tissue as a reliable and efficient source for generating mesenchymal stem cells (MSC). METHODS: MSC were generated from human placenta tissue by enzymatic digestion and mechanical dissociation. The placenta MSC (PLC-MSC) were characterized for expression of cell surface markers, embryonic stem cell (ECS) gene expression and their differentiation ability into adipocytes and osteocytes. The immunosuppressive properties of PLC-MSC on resting and phytohemagglutinin (PHA) stimulated allogenic T cells were assessed by means of cell proliferation via incorporation of tritium thymidine (3H-TdR). RESULTS: The generated PLC-MSC appeared as spindle-shaped cells, expressed common MSC surface markers and ESC transcriptional factors. They also differen-tiated into adipogenic and osteogenic lineages when induced. However, continuous cultivation up to passage 15 caused changes in morphological appearance and cellular senescence, although the stem cell nature of their protein expression was unchanged. In terms of their immunosuppressive properties, PLC-MSC were unable to stimulate resting T cell proliferation; they inhibited the PHA stimulated T cells in a dose dependent manner through cell to cell contact. In our study, MSC generated from human placenta exhibited similar mesenchymal cell surface markers; MSC-like gene expression pattern and MSC-like differentiation potential were comparable to other sources of MSC. CONCLUSION: We suggest that placenta tissues can serve as an alternative source of MSC for future experimental and clinical studies. | Shalini Vellasamy Pratheep Sandrasaigaran Sharmili Vidyadaran Elizabeth George Rajesh Ramasamy | 2012 | World Journal of Stem Cells2012，4，6： | 5 |
| 2 | An Overview on the Degradability of Polymer Nanocomposites 显示文摘 | Jitendra K Pandey K Raghunatha Reddy A Pratheep Kumar | 2005 | Polymer Degradation and Stability2005，88，： | 1 |
| 3 | An overview on the degr- adability of polymer nano-composites 显示文摘 | PANDEY J JITENDRA K PRATHEEP K | 2004 | Polymer Degradation and Stability2004，，5： | 1 |
| 4 | An Overview on the Degradability of Polymer Nanocomposites显示文摘 | JITENDRA K P RAGHUNATHA R K PRATHEEP K A | 2005 | Polymer Degradation and Stability2005，88，： | 1 |
| 5 | Degradability of composites prepared from ethylene-propylene copolymer and jute fiber under accelerated aging and biotic environments显示文摘 | Annamalai Pratheep Kumar Raj Pal Singh Bhimrao D Sarwade | 2005 | Materials Chemistry and Physics2005，92，： | 1 |
| 6 | An overview on the degradability of polymer nanocomposites显示文摘 | Jitendra K Pandey Raghunatha K R Pratheep A K | 2005 | Polymer Degradation and Stability2005，88，： | 1 |
| 7 | A mixed acid methodology to produce thermally stable cellulose nanocrystal at high yield using phosphoric acid显示文摘Cellulose nanocrystal(CNC)with distinctive shape-morphology,enhanced thermal stability and dispersibility is essential for overcoming the challenges in processing polymer/CNC nanocom-posites through melt compounding at elevated temperatures.This study shows a mixed acid hy-drolysis method to produce CNC with improved thermal stability and high productivity.The use of phosphoric acid(H_(3)PO_(4)),as a mild acid,in combination with a strong acid either sulphuric acid(H_(2)SO_(4))or hydrochloric acid(HCl)leads to reduced use of strong acids and low impact on our environment.The influences of acid combination and sequence of addition on the production yield were investigated by retaining the proportion of H_(3)PO_(4)to corrosive acid(H_(2)SO_(4)and HCl)4 to 1,and solid to liquid ratio 1꞉75.This methodology has enabled to isolate CNC with higher thermal stability,dispersibility and productivity in terms of amount acid used 1 g of CNC,as compared with single acid hydrolysis.The CNC produced using the combination of H_(3)PO_(4)and HCl exhibits high thermal stability,dispersibility and rod-like shape morphology with length and width of(424±86)and(22±3)nm,respectively.Moreover,this approach has reduced H_(3)PO_(4)consumption by 54%as compared with single acid hydrolysis method for the production of same amount of CNC. | Khairatun Najwa Mohd Amin Alireza Hosseinmardi Darren J.Martin Pratheep K.Annamalai | 2022 | Journal of Bioresources and Bioproducts2022，7，2： | 1 |
| 8 | Degradability of composites, prepared from ethylene- propylene copolymer and jute fiber under accelerated aging and biotic environments 显示文摘 | Kumar Annamalai Pratheep Singh Raj Pal Sarwade Bhimrao D | 2005 | Materials Chemistry and Physics2005，92，23： | 1 |
| 9 | Degradability of composites,prepared from ethylene-propylene copolymer and Jute fiber under accelerated aging and biotic environments显示文摘 | Annamalai Pratheep Kumar Raj Pal Singh Bhimrao D Sarwade | 2005 | Materials Chemistry and Physics2005，92，： | 1 |
| 10 | An overview on the degradability of polymer nanocomposites显示文摘 | Jitendra K. Pandey K. Raghunatha Reddy A. Pratheep Kumar R.P. Singh | 2004 | Polymer Degradation and Stability2004，，2： | 1 |
| 11 | An overview on the degradability of polymer nanocomposites显示文摘 | Pandey Jitendra K Raghunatha Reddy K Pratheep Kumar A | 2005 | Polymer Degradation and Stability2005，88，： | 1 |
| 12 | Degradability of composites prepared from ethylene-propylene copolymer and jute fiber under accelerated aging and biotic environments显示文摘 | Annamalai Pratheep Kumar Raj Pal Singh Bhimrao D Sarwade | 2005 | Materials Chemistry and Physics2005，92，： | 1 |
| 13 | Nanoscale particles for polymer degradation and stabilization-Trends and future perspectives显示文摘 | Annamalai Pratheep Kumar Dilip Depan NamrataSingh Tomer | 2009 | Progress in Polymer Science2009，34，47： | 1 |
| 14 | An overview on the degradability of polymer nanocomposites 显示文摘 | PANDEY J K RAGHUNATHA R K PRATHEEP K A | 2005 | Polym Degrad Stab2005，88，： | 1 |
| 15 | An overview on the Degradability of Polymer Nanocomposites 显示文摘 | jitendra K Pandey K Raghunatha Reddy A Pratheep Kumar | 2005 | Polymer degradation and Stability2005，88，： | 1 |
| 16 | 家蚕末龄幼虫、蛹和成虫期载脂蛋白组成的变化(英文)显示文摘【目的】载脂蛋白是一种重要的血淋巴脂蛋白,它将90%的脂类输送到内部器官。本研究旨在调查家蚕Bombyx mori各个发育期血淋巴载脂蛋白组成变化。【方法】采用溴化钾密度梯度超离心、肝素钠沉淀(heparin sodium precipitation)和连接蒸发光散射检测器的高效液相色谱(high performance liquid chromatography coupled with evaporative light scattering detector,HPLC-ELSD)等方法,检测了家蚕各个发育期血淋巴脂蛋白组成变化。【结果】从5龄幼虫到成虫的转变过程中,从幼虫期和吐丝期家蚕中分离的载脂蛋白密度分别为1.130和1.139 g/mL,但蛹期载脂蛋白密度降低(为1.083 g/mL)。应用肝素钠沉淀法对载脂蛋白组成的分析表明,家蚕吐丝期载脂蛋白主要是高密度载脂蛋白(9.8μg/μL);从吐丝期开始,低密度载脂蛋白含量增加,并在蛹期持续存在(4.2μg/μL)。在所有发育期,载脂蛋白Ⅲ含量低,在2日龄蛹中含量最高(4.0μg/μL)。另外,从吐丝期至成虫期,总脂浓度逐渐增加,从1.4μg/μL增加至3.8μg/μL。通过HPLC-ELSD对中性脂组成的分析表明,在所有发育期只含有微量的单酰甘油(<0.04μg/μL),在5龄幼虫中期至蛹期末观察到二酰甘油(0.11~0.61μg/μL),在蛹期发现三酰甘油(0.18~1.02μg/μL)。【结论】总体结果说明,在幼虫期和吐丝期,高密度载脂蛋白主要运输二酰甘油;化蛹后,高密度载脂蛋白更多地与三酰甘油有关,而与二酰甘油相关性小,并与载脂蛋白Ⅲ形成低密度载脂蛋白有关。这些结果提示载脂蛋白的转变是一个精确的时间控制过程。 | Thangaraj PRATHEEP Neelamegam RAMESHKUMAR Nagarajan KAYALVIZHI Thangaiyan SUGANYA Sivashanmugam KARTHIKEYAN Muthukalingan KRISHNAN | 2017 | 昆虫学报2017，60，3： | 0 |
| 17 | Preliminary study on overproduction of reactive oxygen species by neutrophils in diabetes mellitus显示文摘AIM:To assess the amount and pattern of reactive oxygen species(ROS)production in diabetic patientderived neutrophils.METHODS:Blood samples from type 2 diabetes mellitus(DM)patients and volunteers(controls)were subjected to neutrophil isolation and the assessment of neutrophil oxidative burst using chemiluminescence assay.Neutrophils were activated by using phorbol myristate acetate(PMA)and neutrophils without activation were kept as a negative control.The chemilu-minescence readings were obtained by transferring cell suspension into a 1.5 m L Eppendorf tube,with PMA and luminol.Reaction mixtures were gently vortexed and placed inside luminometer for a duration of 5 min.RESULTS:Our results showed that in the resting condition,the secretion of ROS in normal non-diabetic individuals was relatively low compared to diabetic patients.However,the time scale observation revealed that the secreted ROS declined accordingly with time in non-diabetic individuals,yet such a reduction was not detected in diabetic patients where at all the time points,the secretion of ROS was maintained at similar magnitudes.This preliminary study demonstrated that ROS production was significantly higher in patients with DM compared to non-diabetic subjects in both resting and activated conditions.CONCLUSION:The respiratory burst activity of neutrophils could be affected by DM and the elevation of ROS production might be an aggravating factor in diabetic-related complications. | Noridzzaida Ridzuan Cini Mathew John Pratheep Sandrasaigaran Maryam Maqbool Lee Chuen Liew Jonathan Lim Rajesh Ramasamy | 2016 | World Journal of Diabetes2016，7，13： | 0 |
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