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| 1 | Tenascin C upregulates interleukin-6 expression in human cardiac myofibroblasts via toll-like receptor 4显示文摘AIM:To investigate the effect of Tenascin C(TNC)on the expression of pro-inflammatory cytokines and matrixmetalloproteinases in human cardiac myofibroblasts(CMF).METHODS:CMF were isolated and cultured from patients undergoing coronary artery bypass grafting.Cultured cells were treated with either TNC(0.1μmol/L,24 h)or a recombinant protein corresponding to different domains of the TNC protein;fibrinogen-like globe(FBG)and fibronectin typeⅢ-like repeats(TNⅢ5-7)(both 1μmol/L,24 h).The expression of the proinflammatory cytokines;interleukin(IL)-6,IL-1β,TNFαand the matrix metalloproteinases;MMPs(MMP1,2,3,9,10,MT1-MMP)was assessed using real time RT-PCR and western blot analysis.RESULTS:TNC increased both IL-6 and MMP3(P<0.01)mR NA levels in cultured human CMF but had no significant effect on the other markers studied.The increase in IL-6 mR NA expression was mirrored by an increase in protein secretion as assessed by enzymelinked immunosorbant assay(P<0.01).Treating CMF with the recombinant protein FBG increased IL-6mR NA and protein(P<0.01)whereas the recombinant protein TNⅢ5-7 had no effect.Neither FBG nor TNⅢ5-7 had any significant effect on MMP3 expression.The expression of toll-like receptor 4(TLR4)in human CMF was confirmed by real time RT-PCR,western blot and immunohistochemistry.Pre-incubation of cells with TLR4neutralising antisera attenuated the effect of both TNC and FBG on IL-6 mR NA and protein expression.CONCLUSION:TNC up-regulates IL-6 expression in human CMF,an effect mediated through the FBG domain of TNC and via the TLR4 receptor. | Azhar Maqbool Emma J Spary Iain W Manfield Michaela Ruhmann Lorena Zuliani-Alvarez Filomena O Gamboa-Esteves Karen E Porter Mark J Drinkhill Kim S Midwood Neil A Turner | 2016 | World Journal of Cardiology2016，8，5： | 4 |
| 2 | Mechanical properties of a biodegradable bone regeneration scaffold显示文摘 | Porter B D Oldham J B He S L | 2000 | J Biomech Eng2000，122，3： | 2 |
| 3 | Cardiac troponin 1 in pediatrics:normal values and potential use in the assessmant of cardiac injury 显示文摘 | Hirsch R Landt Y Porter S | 1997 | J Pediatr1997，130，6： | 1 |
| 4 | Mechanisms of free radical oxidation of unsaturated lipids 显示文摘 | Porter N A Caldwell S E Mills K A | 1995 | Lipids1995，30，4： | 1 |
| 5 | ADAMTS8 and ADAMTS15 ex- pression predicts survival in human breast carcinoma显示文摘 | Porter S Span PN Sweep FC | 2006 | Int J Canc- er2006，118，5： | 1 |
| 6 | The effects of conflict, trust and task commitment on project team performance显示文摘 | PORTER T W LILLY B S | 1996 | The InternationalJournal of Conflict Management1996，7，： | 1 |
| 7 | The determinants of national innovative capacity显示文摘 | FurmanJ L Porter ME Stern S | 2002 | Research Policy2002，31，6： | 1 |
| 8 | Biomaterial developments for bone tissue engineering显示文摘 | Burg KJ Porter S Kellam JF | | 0，，23： | 1 |
| 9 | The Determinants of National Innovative Capacity显示文摘 | FURMAN J L PORTER M E STERN S | 2002 | Research Policy2002，31，8： | 1 |
| 10 | Predictors of warm ischemia time and perioperative complications in a multicenter,international series of robot-assisted partial nephrectomy显示文摘 | Ficarra V Bhayani S Porter J | 2012 | Eur Urol2012，61，2： | 1 |
| 11 | Alternative approaches to the employee-organization rela- tionship:does investment in employees pay off显示文摘 | TSUI A S PEARCE J L PORTER L W TRIPOLI A M | 1997 | The A cademy of Management Journal1997，40，5： | 1 |
| 12 | The use of DAPI for identifying and counting aquatic microflora 显示文摘 | Porter K G Feig Y S | 1980 | Limnol Oceanogr1980，25，5： | 1 |
| 13 | The effects of conflict,trust and taskcommitment on Project team Performance显示文摘 | Porter T W Lilly B S | 1996 | The Interna-tional Journal of Conflict Management1996，7，： | 1 |
| 14 | Technology opportunities analysis:integrating technology monitoring, forecasting and assessment with strategic planning显示文摘 | Porter A L Jin X-Y Gilmour J E Cunningham S W Xu H Stanard C WangL | 1994 | SRA Journal (Society of Research Administrators)1994，，2： | 1 |
| 15 | Interaction of diagnostic ultrasound with synthetic oligonucleotide-labeled perfluorocarbon-exposed sonicated dextrose albumin micro bubbles显示文摘 | Porter TR lverson PL Li S | 1996 | J Ultrason Med1996，15，： | 1 |
| 16 | The effect of organic amendments on phosphorus chemistry in a potato cropping systems 显示文摘 | Erich M S Fitzgeald C B Porter G A | 2002 | Agric Ecosys Environ2002，88，1： | 1 |
| 17 | Predicting the thermomechanical properties of an epoxy resin blend as a funetion of temperature and strain rate显示文摘 | FOREMAN J P PORTER D BEHZADI S | 2010 | Composites Part A-Applied Science and Manufacturing2010，41，9： | 1 |
| 18 | Sex ratio variations as a function of host size in Pseudacteon flies(Diptera,Phoridae),parasitoids of Solenopsis fire ants(Hymenoptera,Formicidae)显示文摘 | Morrison L W Porter S D Gilbert L E | 1999 | Biol J Linn Soc1999，66，： | 1 |
| 19 | Correlation of prenatal ultrasound diagnosis and pathologic findings in fetal brain abnormalities显示文摘 | Carroll SG Porter H Abdel-Fattah S | 2000 | Ultrasound Obstet Gynecol2000，16，2： | 1 |
| 20 | Correlation between climate events in the North Atlantic and China during the last glaciation显示文摘 | PORTER S C AN Z S | 1999 | Nature1999，375，： | 1 |
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