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| 1 | Restoring the dampened expression of the core clock molecule BMAL1 protects against compression-induced intervertebral disc degeneration显示文摘The circadian clock participates in maintaining homeostasis in peripheral tissues,including intervertebral discs(IVDs).Abnormal mechanical loading is a known risk factor for intervertebral disc degeneration(IDD).Based on the rhythmic daily loading pattern of rest and activity,we hypothesized that abnormal mechanical loading could dampen the IVD clock,contributing to IDD.Here,we investigated the effects of abnormal loading on the IVD clock and aimed to inhibit compression-induced IDD by targeting the core clock molecule brain and muscle Arnt-like protein-1(BMAL1).In this study,we showed that BMAL1 KO mice exhibit radiographic features similar to those of human IDD and that BMAL1 expression was negatively correlated with IDD severity by systematic analysis based on 149 human IVD samples.The intrinsic circadian clock in the IVD was dampened by excessive loading,and BMAL1 overexpression by lentivirus attenuated compression-induced IDD.Inhibition of the RhoA/ROCK pathway by Y-27632 or melatonin attenuated the compression-induced decrease in BMAL1 expression.Finally,the two drugs partially restored BMAL1 expression and alleviated IDD in a diurnal compression model.Our results first show that excessive loading dampens the circadian clock of nucleus pulposus tissues via the RhoA/ROCK pathway,the inhibition of which potentially protects against compression-induced IDD by preserving BMAL1 expression.These findings underline the importance of the circadian clock for IVD homeostasis and provide a potentially effective therapeutic strategy for IDD. | Dong Wang Pandi Peng Michal Dudek Xueyu Hu Xiaolong Xu Qiliang Shang Di Wang Haoruo Jia Han Wang Bo Gao Chao Zheng Jianxin Mao Chu Gao Xin He Pengzhen Cheng Huanbo Wang Jianmin Zheng Judith A.Hoyland Qing-Jun Meng Zhuojing Luo Liu Yang | 2022 | Bone Research2022，10，2： | 2 |
| 2 | Decoding the annulus fibrosus cell atlas by scRNA-seq to develop an inducible composite hydrogel:A novel strategy for disc reconstruction显示文摘Low back pain is one of the most serious public health problems worldwide and the major clinical manifestation of intervertebral disc degeneration(IVDD).The key pathological change during IVDD is dysfunction of the annulus fibrosus(AF).However,due to the lack of an in-depth understanding of AF biology,the methods to reconstruct the AF are very limited.In this study,the mice AF cell atlas were decoded by single-cell RNA sequencing to provide a guide for AF reconstruction.The results first identify a new population of AF cells,fibrochondrocyte-like AF cells,which synthesize both collagen Ⅰ and collagen Ⅱ and are potential functional cells for AF reconstruction.According to the dual features of the AF extracellular matrix,a composite hydrogel based on the acylation of methacrylated silk fibroin with methacrylated hyaluronic acid was produced.To obtain the ability to stimulate differentiation,the composite hydrogels were combined with a fibrochondrocyte-inducing supplement.Finally,reconstruction of the AF defects,by the novel AF stem cell-loaded composite hydrogel,could be observed,its amount of chondroid matrices recovered to 31.7% of AF aera which is significantly higher than that in other control groups.In summary,this study decodes the AF cell atlas,based on which a novel strategy for AF reconstruction is proposed. | Han Wang Di Wang Beier Luo Dong Wang Haoruo Jia Pandi Peng Qiliang Shang Jianxin Mao Chu Gao Ye Peng Lu Gan Junjie Du Zhuojing Luo Liu Yang | 2022 | Bioactive Materials2022，7，8： | 1 |
| 3 | Marker gene excision in transgenic Brassica napus via Agrobacterium-mediated Cre/lox transient expression system显示文摘Oilseed rape(Brassica napus L.)is one of the most important oil crops in the world.However,study on marker-free transgene of B.napus for bio-safety purpose is limited in this allotetraploid crop.In order to advance marker gene excision research,a newly designed Cre/lox system combining crossing and auto-excision strategy was introduced into B.napus.The system consists of 2 sets of independent vectors including pC35 Spro::T7 RP carrying T7 RNA polymerase and pCT7 pro::Cre carrying T7 promoter respectively.After hybridization of 2 according types of transgenic B.napus,marker gene would be removed as T7 RNA polymerase facilitate T7 promoter to promote Cre gene expression.Totally 52 and 46 positive To transgenic lines of these 2 vectors were obtained after identification by PCR and test trip.T1 plants from 3 T0 positive pC35 Spro::T7 RP lines and 2 T0 positive pC35 Spro::T7 RP lines were identified as single copy according to segregation ratio and were chosen for crossing.However,expression of CP4 EPSPS(glyphosate resistance gene)and OXY(bromoxynil resistance gene)were not found in F1 progeny,which proved that the excision was not complete.The possible reasons for our limited success were investigated and detailed analyses were performed.Although this system is not applicable for generating transgenic B.napus free from selectable marker gene,it provided valuable experience and clue for further improvement of this technique.Many other advantages and further improvement will be progressed in future work. | Pandi Wang Xiaojuan Xiong Ping Fu Gang Wu Fang Liu | 2020 | Oil Crop Science2020，5，2： | 0 |
| 4 | NMR-based metabolomics pro!le analysis in understanding metabolic variations of Bra LTP1-overexpressing Brassica napus显示文摘BraLTP1 is an important member of lipid transfer protein family in Brassica napus.The aim of current study was to detect, classify and follow variations in metabolite profiling of B. napus with overexpression of BraLTP1. In this study, metabolic change in leaves of BraLTP1-overexpressing B. napus plants (BraLTP1-22) and negative control (BraLTP1-22N) was investigated using nuclear magnetic resonance (NMR). Statistical strategy of principle component analysis (PCA) was performed to identify related difference metabolites. PCA score plots indicated not only high reproducibility of various treatments, but also significant difference of metabolite levels between different treatments. PCA loading plots indicated main responsible metabolites and a total of 50 metabolites were quantitatively determined. A wide range of metabolites were detected due to BraLTP1 overexpression,including biosynthesis and metabolism of sugars, amino acids, ammoniums compounds and organic acids. Furthermore, concentrations of 17 amino acids were determined for other set of samples by amino acids component analysis assay. The link between metabolite variations and phenotype were also discussed in BraLTP1-overexpressing B. napus.This work will help to gain insight into BraLTP1 function in B. napus in metabolism. | Nini Tian Pandi Wang Xiaojuan Xiong Ping Fu Gang Wu Fang Liu | 2018 | Oil Crop Science2018，3，1： | 0 |
| 5 | Effects of non-procedural factors in Brassica napus genetic transformation显示文摘Rapeseed (Brassica napus) is the second largest oil crop in the world. However, transformation efficiency of rapeseed still needs to be improved. To evaluate non-procedural factors (e.g. explants, section of explant, marker genes and number of exogenous genes) effects on transformation efficiency, 6-day-old hypocotyl explants from in vitro grown seedlings were co-cultivated with Agrobacterium strain GV3101 harboring a binary vector using optimized transformation procedure. Results showed that normal maturing variety ‘Zhongshuang 6 (ZS6)’ had the highest overall capacity to produce rooted shoots among 5 common varieties and 6 early maturing varieties, with green callus induction rate 81.45% and shoot regeneration rate 21.66%. Early maturing variety 14M645 has relatively high regeneration rate (4.69%) and one of the shortest growth periods (107.54 d). Data showed that choosing neomycin phosphotransferase II gene (NPTII) as selectable marker led to the best transformation rate (17.38%). Selecting upper hypocotyl segments near cotyledon as explant provided the higest transformation efficiency, with regeneration rate of 25.59% when using NPTII as selectable marker and 22.19% for Bar. B. napus transformed with single gene showed higher transformation frequency than vectors with multiple genes,highlighting difficulty of multiple gene transformation. This work helped to further improve genetic transformation of B. napus by optimizing factors that impact transformation efficiency,and it would ultimately improve research in transgenic B. napus varieties with commercial potential. | Fang Liu Xiaojuan Xiong Pandi Wang Li Lei Xinhua Zeng Li Zhu Yunjing Li Junling Luo Donghui Fu Ping Fu Gang Wu | 2017 | Oil Crop Science2017，2，2： | 0 |
| 6 | Nanoagent-based theranostic strategies against human coronaviruses显示文摘The emergence of human coronaviruses(HCoVs),especially the current pandemic caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),engender severe threats to public health globally.Despite the outstanding breakthrough of new vaccines and therapeutic medicines in the past years,HCoVs still undergo unpredictable mutations,thus demanding more effective diagnostic and therapeutic strategies.Benefitting from the unique physicochemical properties and multiple nano-bio interactions,nanomaterials hold promising potential to fight against various HCoVs,either by providing sensitive and economic nanosensors for rapid viral detection,or by developing translatable nanovaccines and broad-spectrum nanomedicines for HCoV treatment.Herein,we systemically summarized the recent applications of nanoagents in diagnostics and therapeutics for HCoV-induced diseases,as well as their limitations and perspectives against HCoV variants.We believe this review will promote the design of innovative theranostic nanoagents for the current and future HCoV-caused pandemics. | Tao Feng Chaofan Nie Pandi Peng Hui Lu Tengjiao Wang Peng Li Wei Huang | 2022 | Nano Research2022，15，4： | 0 |
| 7 | LC-MS based secondary metabolic profile of BraLTP2 overexpressing Brassica napus显示文摘BraLTP2 is an important member of lipid transfer protein family, and its molecular biology function in Brassica napus(B. napus) had been explored by prerious study. However, affection of BraLTP2 on secondary metabolites is still not clear. In this study, we investigated difference of leaf secondary metabolite profiling between BraLTP2 overexpressing B. napus and wild type. Liquid chromatography tandem mass spectrometry(LC-MS) was utilized. Wide range of secondary metabolites was found in BraLTP2 overexpressing plants. A total of 100 secondary metabolites were determined, 42 of which had significant differences, including flavonoids, phenylpropanoids and phenolamides. These results were in accordance with significant increasing trichomes of overexpressing BraLTP2 plants, which might produce and store secondary metabolites. Partial least squares discriminant analysis(PLS-DA) was performed to identify difference of secondary metabolites. PLS-DA score plots showed high reproducibility of each treatment. Significant changes were found between transformed and wild type. Permutation test validates the reliability rigorously. Furthermore, overexpressing of BraLTP2 led to seed germination improvement during the first 48 h under oxidation stress. Increased oxidation resistance of transgenic B. napus was in accordance with the significant variations of phenylpropanoids, phenylpropanoids and phenolamides. | Fang Liu Pandi Wang Nini Tian Xiaojuan Xiong Ping Fu Gang Wu | 2018 | Oil Crop Science2018，3，3： | 0 |
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