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| 1 | Clinical significance of connective tissue growth factor in hepatitis B virus-induced hepatic fibrosis显示文摘AIM:To determine the utility of connective tissue growth factor(CCN2/CTGF) for assessing hepatic fibrosis in hepatitis B virus(HBV)-induced chronic liver diseases(CLD-B).METHODS:Enzyme-linked immunosorbent assay was used to measure CCN2 in sera from 107 patients with chronic hepatitis B(CHB) and 39 patients with HBVinduced active liver cirrhosis and 30 healthy individuals.Liver samples from 31 patients with CHB,8 patients with HBV-induced liver cirrhosis and 8 HBV carriers with normal liver histology were examined for transforming growth factor β-1(TGF-β1) or CCN2 mRNA levels by in situ hybridization,and computer image analysis was performed to measure integrated optimal density(IOD) of CCN2 mRNA-positive cells in liver tissues.Histological inflammation grading and fibrosis staging were evaluated by H and E staining and Van Gieson's method.RESULTS:Serum CCN2 concentrations were,respectively,4.0-or 4.9-fold higher in patients with CHB or active liver cirrhosis as compared to healthy individuals(P < 0.01).There was good consistency between the levels of CCN2 in sera and CCN2 mRNA expression in liver tissues(r = 0.87,P < 0.01).The levels of CCN2 in sera were increased with the enhancement of histological fibrosis staging in patients with CLD-B(r = 0.85,P < 0.01).Serum CCN2 was a reliable marker for the assessment of liver fibrosis,with areas under the receiver operating characteristic(ROC) curves(AUC) of 0.94 or 0.85 for,respectively,distinguishing normal liver controls from patients with F1 stage liver fibrosis or discriminating between mild and significant fibrosis.CONCLUSION:Detection of serum CCN2 in patients with CLD-B may have clinical significance for assessment of severity of hepatic fibrosis. | Rong-Li Piao David R Brigstock Jie Zhu Man-Li Zhang Run-Ping Gao | 2012 | World Journal of Gastroenterology2012，18，18： | 12 |
| 2 | An immortalized rat pancreatic stellate cell line RP-2 as a new cell model for evaluating pancreatic fibrosis,inflammation and immunity显示文摘BACKGROUND: Pancreatic stellate cells(PSCs) play a critical role in the pathogenesis of pancreatic fibrosis and have emerging functions as progenitor cells,immune cells or intermediaries in pancreatic exocrine secretion. Increasing evidence has shown that desmin as an exclusive cytoskeleton marker of PSC is only expressed in part of these cells. This study was to establish a desmin-positive PSC cell line and evaluate its actions on pancreatic fibrosis,inflammation and immunity.METHODS: The presence of cytoskeletal proteins,integrin α5β1 or TLR4,was determined by immunocytochemistry while the production of desmin,collagen I,MMP-1,MMP-2,TIMP-2,or CD14 was evaluated by Western blotting. The levels of desmin,collagen I,IL-1 and IL-6 m RNA were determined by real-time quantitative PCR. The secretion of cytokines was detected by ELISA. Cell function was assessed using adhesion,migration,or proliferation assays. RESULTS: A stable activated rat PSC cell line(designated as RP-2) was established by RSV promoter/enhancer-driven SV40 large T antigen expression. RP-2 cells retained typical PSC properties,exhibited a myofibroblast-like phenotype and persistently produced desmin. The cells produced collagen I protein,matrix metalloproteinases and inhibitors thereof. RP-2 cells demonstrated typical PSC functions,including proliferation,adherence,and migration,the latter two of which occurred in response to fibronectin and were mediated byintegrin α5β1. TLR4 and its response genes including proinflammatory cytokines(IL-1,IL-6,TNF-α) and chemotactic cytokines(MCP-1,MIP-1α,Rantes) were produced by RP-2 cells and activated by LPS. LPS-induced IL-1 or IL-6 m RNA expression in this cell line was fully blocked with My D88 inhibitor.CONCLUSION: RP-2 cells provide a novel tool for analyzing the properties and functions of PSCs in the pathogenesis of fibrosis,inflammation and immunity in the pancreas. | Rong-Li Piao Ming Xiu David R Brigstock Run-Ping Gao | 2015 | Hepatobiliary & Pancreatic Diseases International2015，14，6： | 6 |
| 3 | Focal autoimmune pancreatitis and chronic sclerosing sialadenitis mimicking pancreatic cancer and neck metastasis显示文摘Type 1 autoimmune pancreatitis(AIP)or chronic sclerosing sialadenitis(Küttner’s tumour)is an uncommon disorder that has recently been confirmed as an Ig G4-related disease.Here,we describe a rare case of a53-year-old male patient who primarily presented with pancreatic body mass,left neck mass and several lumps in his lower lip mimicking pancreatic cancer(PC)and neck metastasis.The patient underwent pancreatic body mass and labial gland lumps resection as well as an ultrasound-guided biopsy of the left neck mass.He was diagnosed with Ig G4-related focal type of AIP(f-AIP)and Küttner’s tumour by immunohistochemistry.The patient responded well to corticosteroid therapy andremains healthy with no signs of recurrence at one year follow-up.The differentiation of f-AIP from PC is very important to avoid unnecessary pancreatic resection. | Li Sun Qiang Zhou David R Brigstock Su Yan Ming Xiu Rong-Li Piao Yan-Hang Gao Run-Ping Gao | 2014 | World Journal of Gastroenterology2014，20，46： | 3 |
| 4 | Baicalein inhibits oxidative stress-induced cellular damage via antioxidant effects 显示文摘 | Kang K A Zhang R Piao M J | 2012 | Toxicol Ind Health2012，28，5： | 1 |
| 5 | Prognostic value of T cell immunoglobulin mucin-3in prostate cancer显示文摘 | Piao Y R Piao L Z Zhu L H | 2013 | Asian Pac J Cancer Prev2013，16，4： | 1 |
| 6 | Fine mapping andcandidate gene analysis of a new mutant gene for panicleapical abortion in rice显示文摘 | AKTER M B PIAO R KIM B | 2014 | Euphytica2014，197，3： | 1 |
| 7 | Inhibitory effects of glycitein on hydrogen peroxide induced cell damage by scavenging reactive oxygen species and inhibiting c-Jun N-terminal kinase 显示文摘 | Kang K A Zhang R Piao M J | 2007 | Free Radic Res2007，41，6： | 1 |
| 8 | Spl28 encodes a clathrin-associated adaptor protein complex 1, medium subunit μ1 (AP1M1) and is responsible for spotted leaf and early senescence in rice ( Oryza sativa )显示文摘 | Qiao Y Jiang W Lee J Park B Choi M Piao R Woo M Roh J Han L Paek N Seo H Koh H | 2009 | New Phytologist2009，185，1： | 1 |
| 9 | Homocysteine enhances clot-pro rooting activity of endothelial cells v/a phosphatidylserine ex ternalization and microparticles formation显示文摘 | Zhu J Xie R Piao X | 2012 | Amino Acids2012，43，12： | 1 |
| 10 | Mushroom tyrosinase inhibition activity of some chromones 显示文摘 | Piao L Z Park H R Park Y K | 2002 | Chem Pharm Bull Tokyo2002，50，： | 1 |
| 11 | Endoplasmic reticnlum stress signaling is involved in silver nanoparticles-induced apoptosis 显示文摘 | Zhang R Piao MJ Kim KC | 2012 | lnt J Biochem Cell Bio12012，44，1： | 1 |
| 12 | SPL28 encodes a clathrin-associated adaptor protein complex 1, medium subunit 1 (AP1M1) and is responsible for spotted leaf and early senescence in rice (Oryza sativa) 显示文摘 | Qiao Y L Jiang W Z Lee J H Park B S Choi M S Piao R H Woo M O Roh J H Han L Z Paek N C Seo H S Koh H J | 2009 | New Phytologist2009，185，1： | 1 |
| 13 | Butin reduces oxidative stress-induced mitochondriial dysfunction via scavenging of reactive oxygen species 显示文摘 | Zhang R Kang K A Piao M J | 2010 | Food Chem Toxicol2010，8，3： | 1 |
| 14 | Antioxidative constituents from Lycopus lucidus显示文摘 | Woo E R Piao M S | 2004 | Arch Phann Res2004，27，2： | 1 |
| 15 | Nonlinear optical and two-photon absorption properties of 1,3,5-tricyano-2,4,6-tris( styryl)- benzene-containing octupolar oligomers 显示文摘 | Cho B R Piao M J Lee S H | 2002 | Chem Eur J2002，8，17： | 1 |
| 16 | Endoplasmic retieulum stress signaling is involved in silver nanoparticles-induced apoptosis显示文摘 | Zhang R Piao M J Kim KC | 2012 | Int J Biochem Cell Biol2012，44，1： | 1 |
| 17 | Antioxidative constituents from Buddleia officinalis 显示文摘 | Piao M S Kim M R Lee D C3 | 2003 | Arch Pharm Res2003，26，6： | 1 |
| 18 | Ultrasound effects on invertase from Aspergillus niger显示文摘 | Vargas L H M Piao A C S Domingos R N Carmona E C | 2004 | World Journal of Microbiology & Biotechnology2004，20，2： | 1 |
| 19 | GPR56 and its related diseases 显示文摘 | Jin Z Luo R Piao X | 2009 | Prog Mol Biol Transl Sci2009，89，： | 1 |
| 20 | Synthesis and anticonvulsant activity of 1-substimted-7-benzyloxy-4,5-dihydro-tfiazole-quinoline显示文摘 | Cui L J Xie Z F Piao H R | 2005 | Biol PharmBull2005，28，： | 1 |
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