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| 1 | CYLD mediates ciliogenesis in multiple organs by deubiquitinating Cep70 and inactivating HDAC6显示文摘睫是毛一般的细胞器从房间表面延长与重要感觉并且活动性功能。睫的缺点能导致作为 ciliopathies 知道的大量人的疾病。然而,控制 ciliogenesis 的分子的机制仍然保持糟糕定义。这里,我们显示出那 cylindromatosis (CYLD ) ,怀有 deubiquitinase 活动的肿瘤 suppressor 蛋白质,在多重机关在主要、能动的睫的集会起一个关键作用。CYLD 猛烈老鼠 polydactyly 展出并且各种各样的睫的缺点,例如在基础身体抛锚和基础身体和 axenomes 的组织的破坏的失败。CYLD 的睫的功能部分从 70 kDa (Cep70 ) 的 centrosomal 蛋白质被归因于它 polyubiquitin 链的 deconjugation,为 Cep70 到的一个要求与 γ 交往;导管素并且在中心体本地化。另外, histone deacetylase 的调停 CYLD 的抑制 6 (HDAC6 ) ,它支持导管素 acetylation,为 CYLD 的睫的功能组成另一机制。HDAC6 的小分子的禁止者能部分在 CYLD 猛烈老鼠救睫的缺点。这些调查结果在 ciliogenesis 的调整强调蛋白质 ubiquitination 的重要性,作为这个过程的一个关键管理者识别 CYLD,并且建议在 ciliopathies 的 CYLD 缺乏的参与。 | Yunfan Yang Jie Ran Min Liu Dengwen Li Yuanyuan Li Xingjuan Shi Dan Meng Junmin Pan Guangshuo Ou Rim Aneja Sbao-Cong Sun Jun Zhou | 2014 | Cell Research2014，24，11： | 8 |
| 2 | Chromophore-assisted laser inactivation in neural development显示文摘Chromophore-assisted laser inactivation(CALI) is a technique that uses photochemically-generated reactive oxygen species to acutely inactivate target proteins in living cells.Neural development includes highly dynamic cellular processes such as asymmetric cell division,migration,axon and dendrite outgrowth and synaptogenesis.Although many key molecules of neural development have been identified since the past decades,their spatiotemporal contributions to these cellular events are not well understood.CALI provides an appealing tool for elucidating the precise functions of these molecules during neural development.In this review,we summarize the principles of CALI,a recent microscopic setup to perform CALI experiments,and the application of CALI to the study of growth-cone motility and neuroblast asymmetric division. | Wei Li Nico Stuurman Guangshuo Ou | 2012 | Neuroscience Bulletin2012，28，4： | 2 |
| 3 | CRISPR-Cas9 knockout screening for functional genomics显示文摘Large-scale genetic screening offers a critical tool to dissect gene function and pathways underlying biological processes and human disease.Despite the success of genome-wide genetic screens in microorganisms such as yeast,the creation of genome-scale homozygous mutant libraries for metazoans with diploid genomes is time and cost consuming and low throughput.RNA interference(RNAi)has been a predominant solution for functional genomics during the past years since a double strand RNA can inactivate gene function by causing sequence-specific degradation of mRNA.However,the RNAi approach has intrinsic limitations because the suppression of target gene expression | SHEN ZhongFu OU GuangShuo | 2014 | Science China(Life Sciences)2014，57，7： | 2 |
| 4 | The glial actin cytoskeleton regulates neuronal ciliogenesis显示文摘 | Hao Zhu Lianwan Chen Yihong Yang Zhiwen Zhu Xianliang Zhang Wei Li Long Miao Yan Zhang Guangshuo Ou | 2017 | Cell Research2017，27，3： | 1 |
| 5 | Transcription factor STOX1 regulates proliferation of inner ear epithelial cells via the AKT pathway显示文摘 | Xiaowei Nie Kaiqing Zhang Li Wang Guangshuo Ou He Zhu Wei‐Qiang Gao | 2015 | Cell Prolif2015，，2： | 1 |
| 6 | CENP-A的Ser68位动态磷酸化调控它在着丝粒区域的细胞周期依赖性装配显示文摘细胞出版社《发育细胞》创刊于2001年,本刊致力于搭建细胞生物学与发育生物学两大学科之间的桥梁。受过专业博士训l练的科学编辑与作者、审稿人、编委会成员共同合作,及时发布横跨两个领域令人振奋的研究成果,强调两个领域之间共同面临的问题以及相互之间的关系。该杂志2015年公布的影响因子为9.708。 | Zhouliang Yu Xiang Zhou Wenjing Wang Wenqiang Deng Junnan Fang Hao Hu Zichen Wang Shangze Li Lei Cui Jing Shen Linhui Zhai Shengyi Peng Jiemin Wong Shuo Dong Zengqiang Yuan Guangshuo Ou Xiaodong Zhang Ping Xu Jizhong Lou Na Yang Ping Chen Rui-Ming Xu 李国红 | 2016 | 科学新闻2016，0，1： | 1 |
| 7 | Cilia regeneration requires an RNA splicing factor from the ciliary base显示文摘Cilia are microtubule-based organelles projected from most eukaryotic cell surfaces performing cell motility and signaling.Several previously recognized non-ciliary proteins play crucial roles in cilium formation and function.Here,we provide additional evidence that the Caenorhabditis elegans RNA splicing factor PRP-8/PRPF8 regulates ciliogen-esis and regeneration from the ciliary base.Live imaging of GFP knock-in animals reveals that the endogenous PRP-8 localizes in the nuclei and the ciliary base.A weak loss-of-function allele of prp-8 affects ciliary structure but with little impact on RNA splicing.Conditional degradation of PRP-8 within ciliated sensory neurons showed its direct and spe-cific roles in cilium formation.Notably,the penetrance of ciliary defects correlates with the reduction of PRP-8 at the ciliary base but not nuclei,and sensory neurons regenerated cilia accompanying PRP-8 recovery from the ciliary base rather than the nuclei.We suggest that PRP-8 at the ciliary base contributes to cilium formation and regeneration. | Kaiming Xu Guangshuo Ou | 2022 | Cell Regeneration2022，11，1： | 0 |
| 8 | Ciliopathy-associated proteins are involved in vesicle distribution in sensory cilia显示文摘Cilia play a wide range of critical roles in regulating cell motility,sensory signaling and metazoan development(Goetz and Anderson,2010;Reiter and Leroux,2017).Both motile cilia and pri-mary cilia consist of a basal body and a microtubule-based axoneme that is encompassed within the ciliary membrane.The formationand maintenance of ciliary structure depends on bidirectional intraflagellar transport(IFT):the kinesin-2 family motor proteins deliver ciliary precursors bound to the IFT particle protein complex from the ciliary base to the tip and the cytoplasmic dynein-2 recy-cles the anterograde IFT-protein machinery and ciliary turnover products back to the base(Scholey,2013;Reiter and Leroux,2017).Defects of ciliary structure and function lead to more than 35 types of systemic disorders in most human organ systems.which are collectively called ciliopathies(Reiter and Leroux,2017).187 established and 241 candidate ciliopathy-associated genes have been identified from the human genome(Reiter and Leroux,2017). | Ming Li Wanzhong He Wei Li Guangshuo Ou | 2019 | Journal of Genetics and Genomics2019，46，5： | 0 |
| 9 | Correction: Cilia regeneration requires an RNA splicing factor from the ciliary base显示文摘Following publication of the original article(Xu and Ou 2022),it is reported that the supplement figure S1-S5 were missing from the article.Additional file 1:Fig.S1 contains only figure legends but no figures. | Kaiming Xu Guangshuo Ou | 2022 | Cell Regeneration2022，11，1： | 0 |
| 10 | Stereotyped distribution of midbody remnants in early C. elegans embryos requires cell death genes and is dispensable for development显示文摘 | Guangshuo Ou Christian Gentili Pierre Gonczy | 2014 | Cell Research2014，24，2： | 0 |
| 11 | The role of apoptosis in Caenorhabditis elegans neuronal differentiation显示文摘Dear Editor,Neuronal apoptosis is considered to be essential for brain development and neurodegenerative disorders and has been a major focus in cell biological and neuroscientific studies since its first recognition a century ago[1].Remarkable progress has been made in defining the molecular and cel- | WU Kai Da TIAN Dong ZHU ZhiWen CHAI YongPing OU GuangShuo | 2015 | Science China(Life Sciences)2015，58，11： | 0 |
| 12 | A paternal protein facilitates sperm RNA delivery to regulate zygotic development显示文摘Sperm contributes essential paternal factors,including the paternal genome,centrosome,and oocyte-activation signals,to sexual reproduction.However,it remains unresolved how sperm contributes its RNA molecules to regulate early embryonic development.Here,we show that the Caenorhabditis elegans paternal protein SPE-11 assembles into granules during meiotic divisions of spermatogenesis and later matures into a perinuclear structure where sperm RNAs localize.We reconstitute an SPE-11 liquid-phase scaffold in vitro and find that SPE-11 condensates incorporate the nematode RNA,which,in turn,promotes SPE-11 phase separation.Loss of SPE-11 does not affect sperm motility or fertilization but causes pleiotropic development defects in early embryos,and spe-11 mutant males reduce m RNA levels of genes crucial for an oocyte-to-embryo transition or embryonic development.These results reveal that SPE-11 undergoes phase separation and associates with sperm RNAs that are delivered to oocytes during fertilization,providing insights into how a paternal protein regulates early embryonic development. | Dongdong Li Shijing Huang Yongping Chai Ruiqian Zhao Jing Gong Qiangfeng Cliff Zhang Guangshuo Ou Wenyu Wen | 2023 | Science China(Life Sciences)2023，66，10： | 0 |
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