| 1 | Inhibitory effect of agmatine on proliferation of tumor cells by modulation of polyamine metabolism显示文摘Aim: To assess the inhibitory effect of agmatine on tumor growth in vivo and tumor cell proliferation in vitro. Methods: The transplanted animal model,[^3H]thymidine incorporation assay, 3-[4,5-dimethythiazol-2-yl]-2,5-diphenyltetrazolium assay, and lactate dehydrogenase (LDH) release assay were performed.Results: Agmatine, at doses of 5-40mg/kg, suppressed the S180 sarcoma tumor growth dose-dependently in mice in vivo and the highest inhibitory ratio reached 31.3% in Kunming mice and 50.0% in Balb/c mice, respectively. Similar results were obtained in the transplanted B16 melanoma tumor model. Agmatine (1-1000μmol/L) was able to attenuate the proliferation of cultured MCF-7 human breast cancer cells in vitro in a concentration-dependent manner and the highest inhibitory ratio reached 50.3% in the [^3H]thymidine incorporation assay.Additionally, in the LDH release assay, spermine (20μmol/L) and spermidine (20μmol/L) increased the LDH release significantly, but agmatine (1-1000μmol/L) did not, indicating that the inhibitory effect of agmatine on the proliferation of MCF was not related to cellular toxicity. In the [^3H]thymidine incorporation assay,putrescine (12.5-100.0μmol/L) could reverse the inhibitory effect of agmatine on the proliferation of MCF concentration-dependently, suggesting that the inhibitory effect of agmatine on the proliferation of MCF might be associated with a decreased level of the intracellular polyamines pool. Conclusion: Agmatine had significant inhibitory effect on transplanted tumor growth in vivo and proliferation of tumor cells in vitro, and the mechanism might be a result of inducing decrease of intracellular polyamine contents. | Ji-fangWANG Rui-binSU NingWU BoXU Xin-qiangLU YinLIU JinLI | 2005 | Acta Pharmacologica Sinica2005，26，5： | 3 |
| 4 | Involvement of Abdominal and Pelvic Lymph Nodes in Non-Hodgkin Lymphoma: the Nodal Distribution in Chinese Patients显示文摘OBJECTIVE To study the distribution of abdominal and pelvic lymphadenopathy in non-Hodgkin lymphoma (NHL) in Chinese patients.METHODS CT images of 241 NHL patients with abdominal and/or pelviclymphadenopathy were reviewed. Among them, clinical and image datafrom 96 patients fulfilled the requirements for the analysis: 1. Abdominal and/or pelvic lymphadenopathy detected by CT in untreated patients (n=74). 2.Recurrent patients: new lesions in abdominal or pelvic lymph nodes whonever had any nodular lesion by previous abdominal and/or pelvic CT (n =14). 3. Treated patients who did not have abdominal and/or pelvic CTpreviously, showed regression of initial disease for at least 6 months afterchemotherapy and subsequently showed abdominal and/or pelviclymphadenopathy (n =8). According to the Clinical Schema for LymphoidTissue, these patients were divided into 3 histologic subtypes: indolent (IL;n=31), aggressive (AL; n=61)and very aggressive (VAL; n=2)lymphoma.The remaining 2 cases were unclassified lymphoma (UCL). Both abdominaland pelvic CT scans were undertaken in 46 patients, abdominal CT only in 47patients and pelvic CT only in 3 patients. Enhanced CT was obtained in 80patients. The anatomic sites involved were designated as retroperitoneal (ie.paraaortic), mesenteric, abdominal (i.e. celiac, paracardiac, gastrohepaticand hepatic hilum, etc.), retrocrural, subdiaphragmatic, common iliac,internal iliac, external iliac and inguinal nodes respectively.RESULTS The lesions located in the retroperitoneum were most common forIL and AL, the incidences being 83.3% (18/25) and 83.1% (49/59)respectively, results being similar. Among those, lymphadenopathydistributed mainly in the retroperitoneum, superior and inferior renal hila,with an incidence of 72.0% (18/25) in IL and 67.3% (33/49) in AL. Pelviclymphadenopathy came next, with the overall incidence of 41.9% (126/301),57.5% (50/87) in IL and 35.5% (76/214) in AL respectively. Mesenteric lymphnodes stood third with the overall incidence of 37.1% (33/89), 43.3% (13/30)in IL and 33.9% (20/59) in AL. Statistical analysis showed that external iliaclymph node involvement to be more common in I L than in AL (P<0.05), whilecomparisons of other groups showed no statistical significance.CONCLUSION For Chinese NHL patients, retroperitoneal lymph nodes weremostly involved, followed by iliac and mesenteric lymphadenopathy, whichwas different from that of the Western countries, The involved retroperitoneallymph nodes in NHL of Chinese patients were predominantly located in thesuperior and inferior renal hilum. | NingWu YingLiu DongmeiLin YuChen MulanShi | 2004 | Chinese Journal of Clinical Oncology2004，1，4： | 0 |
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