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| 1 | MGMT and MLH1 methylation in Helicobacter pylori-infected children and adults显示文摘AIM:To evaluate the association between Helicobacter pylori(H.pylori) infection and MLH1 and MGMT methylation and its relationship with microsatellite instability(MSI).METHODS:The methylation status of the MLH1 and MGMT promoter region was analysed by methylation specific methylation-polymerase chain reaction(MSPPCR) in gastric biopsy samples from uninfected or H.pylori-infected children(n = 50),from adults with chronic gastritis(n = 97) and from adults with gastric cancer(n = 92).MLH1 and MGMT mRNA expression were measured by real-time PCR and normalised to a constitutive gene(β actin).MSI analysis was performed by screening MSI markers at 4 loci(Bat-25,Bat-26,D17S250 and D2S123) with PCR;PCR products were analysed by single strand conformation polymorphism followed by silver staining.Statistical analyses were performed with either the χ 2 test with Yates continuity correction or Fisher's exact test,and statistical significance for expression analysis was assessed using an unpaired Student's t-test.RESULTS:Methylation was not detected in the promoter regions of MLH1 and MGMT in gastric biopsy samples from children,regardless of H.pylori infection status.The MGMT promoter was methylated in 51% of chronic gastritis adult patients and was associated with H.pylori infection(P < 0.05);this region was methylated in 66% of gastric cancer patients,and the difference in the percentage of methylated samples between these patients and those from H.pylori-infected chronic gastritis patients was statistically significant(P < 0.05).MLH1 methylation frequencies among H.pylori-infected and non-infected chronic gastritis adult patients were 13% and 7%,respectively.We observed methylation of the MLH1 promoter(39%) and increased MSI levels(68%) in samples from gastric cancer patients in comparison to samples from H.pylori-infected adult chronic gastritis patients(P < 0.001 and P < 0.01,respectively).The frequency of promoter methylation for both genes was higher in gastric cancer samples than in H.pylori-positive chronic gastritis samples(P < 0.05).The levels of MLH1 and MGMT mRNA were significantly reduced in chronic gastritis samples that were also hypermethylated(P < 0.01).CONCLUSION:In summary,MGMT and MLH1 methylation did not occur in earlier-stage H.pylori infections and thus might depend on the duration of infection. | Marisa C Alvarez Juliana C Santos Nathália Maniezzo Marcelo S Ladeira Artur LC da Silva Isabel CA Scaletsky José Pedrazzoli Jr Marcelo L Ribeiro | 2013 | World Journal of Gastroenterology2013，19，20： | 5 |
| 2 | In-vitro scolicidal activity of Mallotus philippinensis (Lam.) Muell Arg. fruit glandular hair extract against hydatid cyst Echinococcus granulosus显示文摘Objective: To investigate new scolicidal agent from natural resources to cope with the side effects associated with synthetic drugs in Echinococcosis. Methods: The scolicidal potential of methanolic fruit powder extract (10 and 20 mg/mL) of Mallotus philippinensis ( M. philippinensis ) was investigated. Viability of protoscoleces was confirmed by trypan blue exclusion method, where mortality was observed at concentration of 10 and 20 mg/mL in 60 min treatment against Echinococcus granulosus ( E. granulosus ), under in-vitro conditions with reference to the known standard drug Praziquantel . Results: At concentration 10 and 20 mg/mL, the mortality rate was observed 97% and 99% respectively for 60 min treatment; while up to 93% mortality was observed with 20 mg/mL for only 10 min treatment. The concentration above 20 mg/mL for above 2 h showed 100% mortality, irrespective of further incubation. Conclusions: As compared with the standard anti-parasitic drug Praziquantel our extract has significant scolicidal activity with almost no associated side effects. | Mayank Gangwar Vijay C Verma Tryambak D Singh Sushil K Singh RK Goel Gopal Nath | 2013 | Asian Pacific Journal of Tropical Medicine2013，6，8： | 3 |
| 3 | 接受心脏手术的成人患者的体质量指数与死亡率:全国研究的系统评价和荟萃分析显示文摘接受心脏手术的肥胖患者的发病率和死亡率较低,该现象显然不合理,相关机制不明。该研究试图确定在心脏手术中观察到的这个肥胖悖论是否归因于逆流行病学、偏倚或混杂因素。方法:从英国国家成人心脏外科登记处提取自2002年4月至2013年3月期间进行的所有心脏外科手术数据。同时对至2015年6月Medline,Embase,Scopus, | 刘莉 叶鹏 Mariscalco G Wozniak MJ Dawson AG Serraino GF Porter R Nath M Klersy C Kumar T Murphy GJ | 2017 | 中华高血压杂志2017，25，2： | 2 |
| 4 | 查看详情显示文摘 | Nath D C D Bandyopadhyay S Boughton P Yu A | | 0，，： | 1 |
| 5 | What is the role of autolo- gous blood transfusion in major spine surgery? 显示文摘 | Kumar N Chen Y Nath C | 2012 | Am J Or- thop (Belle Mead NJ)2012，41，6： | 1 |
| 6 | Development and validation of a literacy assessment tool for persons with diabetes 显示文摘 | Nath C R Sylvester S T Van Yasek | 2001 | Diabetes Edue2001，27，6： | 1 |
| 7 | Food consumption, utilization, and detoxification enzyme activity of the rice leaffolder larvae after treatment with Dysoxylum triterpenes显示文摘 | Nath S S Choi M Y Paik C H | 2007 | Pesticide Biochemistry and Physiology2007，88，： | 1 |
| 8 | 查看详情显示文摘 | Nath D C D Bandyopadhyay S Yu A | | 0，，： | 1 |
| 9 | Effect of calophyllolide, a nonsteroidal anti-inflammatory agent, on capillary permeability显示文摘 | Saxena R C Nath R Palit G | 1982 | Planta medica1982，44，4： | 1 |
| 10 | Detection of emphysema with computed tomography:correlation with pulmonary function tests and chest radiolography显示文摘 | Sanders C Nath PH Bailay WC | 1988 | Invest Radiol1988，23，4： | 1 |
| 11 | Cellular electrophysiological effects of hyperthermia on isolated guinea pig papillary muscle显示文摘 | Nath S Lynch C III Whayne JG | 1993 | Circulation1993，88，： | 1 |
| 12 | A study on ultrasonic elliptical vibration cutting of tungsten carbide显示文摘 | Nath C Rahman M Neok S | | 0，，09： | 1 |
| 13 | Coupled GCM simulation of atmosphere–ocean variability associated with the zonally asymmetric SST changes in the tropical Indian Ocean显示文摘 | LAU N C NATH M J | 2004 | J Climate2004，17，2： | 1 |
| 14 | Biological action of leptin as an angiogenic factor显示文摘 | Sierra MR Nath AK Murakami C | 1998 | Science1998，281，5383： | 1 |
| 15 | Dyschromatosis universalis hereditaria:a case report 显示文摘 | Udayashankar C Nath AK | 2011 | Dermatol Online J2011，17，2： | 1 |
| 16 | A study on the effect of tool nose radius in ultrasonic elliptical vibration cutting of tungsten carbidel显示文摘 | Nath C Rahman M Nook S | | 0，，09： | 1 |
| 17 | Radiological and pathological changes inhemiballism-hemichores with striatal hypeiinntensity显示文摘 | Nath J Jambhekar K Rao C | 2006 | J Magn Reson Imaging2006，23，4： | 1 |
| 18 | Radiological and pathological changes in hemiballism-hemichorea with striatal hyperintensity显示文摘 | Nath J Jambhekar K Rao C | 2006 | 23(6):5642006，23，6： | 1 |
| 19 | A study on the effect of tool nose radius in ultrasonic elliptical vibration cutting of tungsten carbide 显示文摘 | Nath C Rahman M Neo K S | 2009 | Journal of Materials Processing Technology2009，209，17： | 1 |
| 20 | Congenital onychogryphosis: Leaning tower nail 显示文摘 | Nath AK Udayashankar C | 2011 | Dermatol Online J2011，17，11： | 1 |
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