1篇
您的检索式：作者名="Naresh Vasupalli"
|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Overexpression of PvSVP1,an SVP-like gene of bamboo,causes early flowering and abnormal floral organs in Arabidopsis and rice显示文摘Bamboo is a nontimber woody plant featuring a long vegetative stage and uncertain flowering time.Therefore,the genes belonging to flowering repressors might be essential in regulating the transition from the vegetative to reproductive stage in bamboo.The Short Vegetative Phase(SVP)gene plays a pivotal role in floral transition and development.However,little is known about the bamboo SVP homologues.In this study,Phyllostachys violascens PvSVP1 is isolated by analysis of the P.edulis transcriptome database.Phylogenetic analysis shows that PvSVP1 is closely related to OsMADS55(rice SVP homolog).PvSVP1 is ubiquitously expressed in various tissues,predominantly in vegetative tissues.To investigate the function of PvSVP1,PvSVP1 is overexpressed in Arabidopsis and rice under the influence of the 35S promoter.Overexpression of PvSVP1 in Arabidopsis causes early flowering and produces abnormal petals and sepals.Quantitative real-time PCR reveals that overexpression in Arabidopsis produces an early flowering phenotype by downregulating FLC and upregulating FT and produces abnormal floral organs by upregulating AP1,AP3 and PI expressions.Simultaneously,overexpression of PvSVP1 in rice alters the expressions of flowering-related genes such as Hd3a,RFT1,OsMADS56 and Ghd7 and promotes flowering under field conditions.In addition,PvSVP1 may be a nuclear protein which interacts with PvVRN1 and PvMADS56 on the yeast two-hybrid and BiFC systems.Our study suggests that PvSVP1 may play a vital role in flowering time and development by interacting with PvVRN1 and PvMADS56 in the nucleus.Furthermore,this study paves the way toward understanding the complex flowering process of bamboo. | Shinan Liu Dan Hou Naresh Vasupalli Xinchun Lin | 2023 | Acta Biochimica et Biophysica Sinica2023，55，2： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员