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| 1 | Enhanced Osteogenesis of Nanosized Cobalt-substituted Hydroxyapatite显示文摘 | Nenad Ignjatovic Zorica Aj dukovic Jelena Rajkovic Stevo Najman Dragan Mihailovic Dragan Uskokovic | 2015 | Journal of Bionic Engineering2015，12，4： | 3 |
| 2 | Vascularization and osteogenesis in ectopically implanted bone tissue-engineered constructs with endothelial and osteogenic differentiated adipose-derived stem cells显示文摘BACKGROUND A major problem in the healing of bone defects is insufficient or absent blood supply within the defect.To overcome this challenging problem,a plethora of approaches within bone tissue engineering have been developed recently.Bearing in mind that the interplay of various diffusible factors released by endothelial cells(ECs)and osteoblasts(OBs)have a pivotal role in bone growth and regeneration and that adjacent ECs and OBs also communicate directly through gap junctions,we set the focus on the simultaneous application of these cell types together with platelet-rich plasma(PRP)as a growth factor reservoir within ectopic bone tissue engineering constructs.AIM To vascularize and examine osteogenesis in bone tissue engineering constructs enriched with PRP and adipose-derived stem cells(ASCs)induced into ECs and OBs.METHODS ASCs isolated from adipose tissue,induced in vitro into ECs,OBs or just expanded were used for implant construction as followed:BPEO,endothelial and osteogenic differentiated ASCs with PRP and bone mineral matrix;BPUI,uninduced ASCs with PRP and bone mineral matrix;BC(control),only bone mineral matrix.At 1,2,4 and 8 wk after subcutaneous implantation in mice,implants were extracted and endothelial-related and bone-related gene expression were analyzed,while histological analyses were performed after 2 and 8 wk.RESULTS The percentage of vascularization was significantly higher in BC compared to BPUI and BPEO constructs 2 and 8 wk after implantation.BC had the lowest endothelial-related gene expression,weaker osteocalcin immunoexpression and Spp1 expression compared to BPUI and BPEO.Endothelial-related gene expression and osteocalcin immunoexpression were higher in BPUI compared to BC and BPEO.BPEO had a higher percentage of vascularization compared to BPUI and the highest CD31 immunoexpression among examined constructs.Except Vwf,endothelial-related gene expression in BPEO had a later onset and was upregulated and well-balanced during in vivo incubation that induced late onset of Spp1 expression and pronounced osteocalcin immunoexpression at 2 and 8 wk.Tissue regression was noticed in BPEO constructs after 8 wk.CONCLUSION Ectopically implanted BPEO constructs had a favorable impact on vascularization and osteogenesis,but tissue regression imposed the need for discovering a more optimal EC/OB ratio prior to considerations for clinical applications. | Jelena G Najdanović Vladimir J Cvetković Sanja T Stojanović MarijaĐVukelić-Nikolić Jelena MŽivković Stevo J Najman | 2021 | World Journal of Stem Cells2021，13，1： | 3 |
| 3 | Analysis of the in vitro degradation and the in vivo tissue response to bi-layered 3D-printed scaffolds combining PLA and biphasic PLA/bioglass components--Guidance of the inflammatory response as basis for osteochondral regeneration显示文摘The aim of the present study was the in vitro and in vivo analysis of a bi-layered 3D-printed scaffold combining a PLA layer and a biphasic PLA/bioglass G5 layer for regeneration of osteochondral defects in vivo Focus of the in vitro analysis was on the(molecular)weight loss and the morphological and mechanical variations after immersion in SBF.The in vivo study focused on analysis of the tissue reactions and differences in the implant bed vascularization using an established subcutaneous implantation model in CD-1 mice and established histological and histomorphometrical methods.Both scaffold parts kept their structural integrity,while changes in morphology were observed,especially for the PLA/G5 scaffold.Mechanical properties decreased with progressive degradation,while the PLA/G5 scaffolds presented higher compressive modulus than PLA scaffolds.The tissue reaction to PLA included low numbers of BMGCs and minimal vascularization of its implant beds,while the addition of G5 lead to higher numbers of BMGCs and a higher implant bed vascularization.Analysis revealed that the use of a bi-layered scaffold shows the ability to observe distinct in vivo response despite the physical proximity of PLA and PLA/G5 layers.Altogether,the results showed that the addition of G5 enables to reduce scaffold weight loss and to increase mechanical strength.Furthermore,the addition of G5 lead to a higher vascularization of the implant bed required as basis for bone tissue regeneration mediated by higher numbers of BMGCs,while within the PLA parts a significantly lower vascularization was found optimally for chondral regeneration.Thus,this data show that the analyzed bi-layered scaffold may serve as an ideal basis for the regeneration of osteochondral tissue defects.Additionally,the results show that it might be able to reduce the number of experimental animals required as it may be possible to analyze the tissue response to more than one implant in one experimental animal. | Mike Barbeck Tiziano Serra Patrick Booms Sanja Stojanovic Stevo Najman Elisabeth Engel Robert Sader Charles James Kirkpatrick Melba Navarro Shahram Ghanaati | 2017 | Bioactive Materials2017，2，4： | 3 |
| 4 | Watershed cuts:minimum spanning forests and the drop of water principle显示文摘 | Cousty J Bertrand G Najman L Couprie M | 2009 | IEEE Trans Pattern Anal Mach Intell2009，31，8： | 1 |
| 5 | In Situ generation of palladium nanoparticles:A simple and highly active protocol for oxygen-pro- moted ligand-free Suzuki coupling reaction of aryl chlorides显示文摘 | CHO J K NAJMAN R DEAN T | 2007 | American Ceramic Society Bulletin2007，9，40： | 1 |
| 6 | Study of interaction of EP and AW additives with dispersants using XANES 显示文摘 | Zhang Z Najman M Kasrai M | 2005 | Tribology Letters2005，18，1： | 1 |
| 7 | Watershed cuts:thinnings, shortest path forests, and topological watersheds显示文摘 | Cousty J Bertrand G Najman L Couprie M | 2010 | IEEE Trans Pattern Anal Mach Intell2010，32，5： | 1 |
| 8 | The detrital record of orogenesis: A review of approaches and techniques used in the Himalayan sedimentary basins 显示文摘 | Najman Y | 2006 | Earth Science Reviews2006，74，： | 1 |
| 9 | Geodesic saliency of watershed contours and hierarchical segmentation 显示文摘 | Schmitt M | 1998 | IEEE Trans Pattern Anal Machine In tell1998，18，12： | 1 |
| 10 | Watershed cuts:minimum span-ning forests and the drop of water principle显示文摘 | Cousty J Bertrand G Najman L | 2009 | IEEE Transactions onPattern Analysis and Machine Intelligence2009，31，8： | 1 |
| 11 | Caudal epidural anesthesia: an anesthetic technique exclusive for pediatric use? Is it possible to use it in adults? What is the role of the ultrasound in this context 显示文摘 | Najman IE Frederieo TN Segurado AV | 2011 | Rev Bras Anestesiol2011，61，1： | 1 |
| 12 | Learning hierarchical features for scene labeling显示文摘 | FARABET C COUPRIE C NAJMAN L | 2013 | IEEE Transactions on Pattern Analysis and Machine Intelligence2013，35，8： | 1 |
| 13 | Detrital geochronology and geochemistry of Cretaceous-Early Miocene strata of Nepal:implications for timing and diachroneity of initial Himalayan orogenesis显示文摘 | DECELLES P G GEHRELS G E NAJMAN Y | 2004 | Earth and Planetary Science Letters2004，227，34： | 1 |
| 14 | A study of HAp/ PLLA composite as a substitute for bone powder using FTIR spectroscopy显示文摘 | Ignjatovic N Savic V Najman S | 2001 | Biomaterials2001，22，： | 1 |
| 15 | X-ray Absorption Spectroscopy and Atomic Force Microscopy of Films Generated from Organosulfur Extreme-Pressure (EP) Oil Additives显示文摘 | M.N. Najman M. Kasrai G.M. Bancroft | 2003 | Tribology Letters2003，，4： | 1 |
| 16 | Early life predictors of childhood intelligence:Findings from the Mater-University study of pregnancy and its outcomes显示文摘 | Lawlor D A Najman J M Batty G D | 2006 | Paediatr Perinat Epidemiol2006，20，2： | 1 |
| 17 | Study of the Chemistry of Films Generated from Phosphate Ester Additives on 52100 Steel Using X-ray Absorption Spectroscopy显示文摘 | M.N. Najman M. Kasrai G.M. Bancroft A. Miller | 2002 | Tribology Letters2002，，3： | 1 |
| 18 | From crowd simulation to airbag deployment: Particle systems, a new paradigm of simulation 显示文摘 | Bouvier E Cohen E Najman L | 1997 | Journal of Electronic Imaging1997，6，1： | 1 |
| 19 | Paraplegia after intratheeal injection of methotrexate 显示文摘 | Moise A G-orin NC Najman A | 1979 | Nouv Presse Med1979，8，9： | 1 |
| 20 | Study of the chemistry of films generated from phosphate ester additives on 52100 steel using X-ray absorption speetroscopy 显示文摘 | Najman M N Kasrai M Bancroft G M | 2002 | Tribology Letters2002，13，3： | 1 |
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