| 1 | MdGSTF6,activated by MdMYB1,plays an essential role in anthocyanin accumulation in apple显示文摘Anthocyanins are biosynthesized on the cytosolic surface of the endoplasmic reticulum and then transported into the vacuole for storage.Glutathione S-transferases(GSTs)are considered to be responsible for the transport of anthocyanins into the vacuole.However,the regulatory mechanisms of GSTs in plants are still unclear.Here,we performed a genome-wide analysis and identified 69 GST genes in apple.The expression of MdGSTF6 was positively correlated with the anthocyanin content(r=0.949)during‘Yanfu 8’fruit development.The overexpression of MdGSTF6 in the Arabidopsis thaliana tt19 mutant resulted in seedlings of 35S::MdGSTF6-GFP/tt19 that could accumulate anthocyanin and rescue its phenotype,suggesting that MdGSTF6 was an anthocyanin transporter.The silencing of MdGSTF6 affected anthocyanin accumulation in apple fruit.Moreover,the knockdown of MdGSTF6 by RNA interference in cultured‘Gala’seedlings inhibited anthocyanin accumulation.The interaction experiments showed that MdMYB1 could bind directly to the MdGSTF6 promoter to transcriptionally activate its expression.Collectively,our results demonstrate that MdGSTF6 encodes an important GST transporter of anthocyanins in apple fruit and provide evidence for the associated regulatory mechanisms.Therefore,MdMYB1 can not only regulate anthocyanin synthesis,but also control the transport of anthocyanin in apples.This information may be useful for further clarifying the regulation of anthocyanin transport in apple. | Shenghui Jiang Min Chen Naibo He Xiaoliu Chen Nan Wang Qingguo Sun Tianliang Zhang Haifeng Xu Hongcheng Fang Yicheng Wang Zongying Zhang Shujing Wu Xuesen Chen | 2019 | Horticulture Research2019，6，1： | 16 |
| 2 | Pulse-burst laser-based 10kHz Thomson scattering measurements显示文摘Thomson scattering (TS), as a popular and reliable diagnostic technique, has successfully measured electron temperatures and electron number densities of plasmas for many years. However, conventional TS techniques using Nd:YAG lasers operate only at tens of hertz. Here, we present the development of a high-repetition-rate TS instrument based on a high-speed, pulse-burst laser system to greatly increase the temporal resolution of measurements. Successful instrument prototype testing was carried out by collecting TS light from laboratory helium and argon plasmas at 10 kHz. Calibration of the instrument detection sensitivity using nitrogen/ oxygen rotational Raman scattering signal is also presented. Quantitative electron number densities and electron temperatures of the plasma were acquired at 10 kHz, for stable plasma discharges as, respectively,~0.9 eV and ~5.37×10^21 m^-3 for the argon plasma, and ~1 eV and ~6.5×10^21 m^-3 for the helium plasma. | Zichen HE Cary SMITH Zhili ZHANG Theodore M BIEWER Naibo JIANG Paul S HSU Sukesh ROY | 2019 | Plasma Science and Technology2019，21，10： | 0 |
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