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| 1 | aunalysis of the Genome Sequence of the Medicinal Plant Salvia miltiorrhiza显示文摘 | Haibin Xu Jingyuan Song Hongmei Luo Yujun Zhang Qiushi Li Yingjie Zhu Jiang Xu Ying Li Chi Song Bo Wang Wei Sun Guoan Shen Xin Zhang Jun Qian Aijia Ji Zhichao Xu Xiang Luo Liu He Chuyuan Li Chao Sun Haixia Yah Guanghong Cui Xiwen Li Xian 'en Li Jianhe Wei Juyan Liu Yitao Wang Alice Hayward David Nelson Zemin Ning Reuben J. Peters Xiaoquan Qi Shilin Chen | 2016 | Molecular Plant2016，9，6： | 70 |
| 2 | Cardiomyocyte overexpression of miR-27b induces cardiac hypertrophy and dysfunction in mice显示文摘最近的研究开始在心脏的肥大和机能障碍的致病揭示了 microRNAs (miRNAs ) 的关键角色。在这研究,我们测试了是否转变生长 factor-β(TGF-β) 调整 miRNA 在心脏的肥大和心失败(HF ) 的发展起了一个枢轴的作用。我们观察到 miR-27b 是在 cardiomyocyte 特定的 Smad4 猛烈老鼠的心的 upregulated,它开发了心脏的肥大。在 vitro,实验证明 miR-27b 表示能被 TGF-β 禁止; 1 并且它的 overexpression 支持了 hypertrophic 房间生长,当 miR-27b 抑制导致了 hypertrophic 房间的抑制时,生长由 phenylephrine (PE ) 引起了处理。而且,有 miR-27b 的 cardiomyocyte 特定的 overexpression 的转基因的老鼠的分析表明 miR-27b overexpression 是足够的导致心脏的肥大和机能障碍。我们验证了 peroxisome 激活 proliferator 的 receptor-γ(PPAR-γ) 作为在 cardiomyocyte 的 miR-27b 的一个直接目标。一致地, miR-27b 转基因的老鼠显著地显示了 PPAR-γ 的底层;比控制鼠标。而且,在用在一只 pressure-overload-induced 老鼠的特定的 antagomir 的 miR-27b 的 vivo silencing, HF 的模型增加了心脏的 PPAR-γ表示,稀释心脏的肥大和机能障碍。我们的学习的结果表明那 TGF-β 1-regulated miR-27b 涉及心脏的肥大的规定,并且为心脏病作为一个有效治疗学的目标验证 miR-27b。 | Jian Wang Yao Song Yan Zhang Han Xiao Qiang Sun Ning Hou Shuilong Guo Youliang Wang Kaiji Fan Dawei Zhan Lagabaiyila Zha Yang Cao Zhenhua Li Xuan Cheng Youyi Zhang Xiao Yang | 2012 | Cell Research2012，22，3： | 36 |
| 3 | Variation of Cd concentration in various rice cultivars and derivation of cadmium toxicity thresholds for paddy soil by species-sensitivity distribution显示文摘It is imperative to derive an appropriate cadmium(Cd) health risk toxicity threshold for paddy soils to ensure the Cd concentration of rice grains meet the food safety standard. In this study, 20 rice cultivars from the main rice producing areas in China were selected, and a pot-experiment was conducted to investigate transformation of Cd in paddy soil-rice system with 0(CK), 0.3 mg kg–1(T1) and 0.6 mg kg–1(T2) Cd treatments in greenhouse. The results showed that Cd concentrations of rice grains existed significant difference(P<0.05) in 20 rice cultivars under the same Cd level in soil. The Cd concentrations of rice grains of the CK, T1 and T2 treatments were in the range of 0.143–0.202, 0.128–0.458 and 0.332–0.806 mg kg–1, respectively. Marked differences of the ratios of Cd concentration for soil to rice grain(BCFs) and transfer factors(TFs, root to grain and straw to grain) among the tested cultivars were observed in this study. The bioconcentration factors(BCFgrain) and TFs of the 20 rice cultivars were 0.300–1.112 and 0.342–0.817, respectively. The TFs of Cd from straw to grain ranged from 0.366 to 1.71, with significant differences among these 20 rice cultivars. The bioconcentration factors(BCFgrain) and TFs among the 20 rice cultivars ranged from 0.300–1.112 and 0.342–0.817, respectively. The species-sensitivity distribution(SSD) of Cd sensitivity of the rice species could be fitted well with Burr-III(R2=0.987) based on the data of BCFs. The toxicity threshold of Cd derived from SSD for the paddy soil was 0.507 mg kg–1 in the present study. | SONG Wen-en CHEN Shi-bao LIU Ji-fang CHEN Li SONG Ning-ning LI Ning LIU Bin | 2015 | Journal of Integrative Agriculture2015，14，9： | 33 |
| 4 | PROTAC-induced BTK degradation as a novel therapy for mutated BTK C481S induced ibrutinib-resistant B-cell malignancies显示文摘 | Yonghui Sun Xingwang Zhao Ning Ding Hongying Gao Yue Wu Yiqing Yang Meng Zhao Jinseok Hwang Yuqin Song Wanli Liu Yu Rao | 2018 | Cell Research2018，28，7： | 29 |
| 5 | Sleep and Cognitive Abnormalities in Acute Minor Thalamic Infarction显示文摘In order to characterize sleep and the cognitive patterns in patients with acute minor thalamic infarction(AMTI), we enrolled 27 patients with AMTI and 12 matched healthy individuals. Questionnaires about sleep and cognition as well as polysomnography(PSG) were performed on days 14 and 90 post-stroke. Compared to healthy controls, in patients with AMTI, hyposomnia was more prevalent; sleep architecture was disrupted as indicated by decreased sleep efficiency, increased sleep latency, and decreased non-rapid eye movement sleep stages 2 and 3;more sleep-related breathing disorders occurred; and cognitive functions were worse, especially memory. While sleep apnea and long-delay memory recovered to a large extent in the patients, other sleep and cognitive function deficit often persisted. Patients with AMTI are at an increased risk for hyposomnia, sleep structure disturbance,sleep apnea, and memory deficits. Although these abnormalities improved over time, the slow and incomplete improvement suggest that early management should be considered in these patients. | Wei Wu Linyang Cui Ying Fu Qianqian Tian Lei Liu Xuan Zhang Ning Du Ying Chen Zhijun Qiu Yijun Song Fu-Dong Shi Rong Xue | 2016 | Neuroscience Bulletin2016，32，4： | 28 |
| 6 | Up-regulation of divalent metal transporter 1 in 6-hydroxydopamine intoxication is IRE/IRP dependent显示文摘铁在 Parkinson 的疾病(PD ) 起一个关键作用。substantia nigra (SN ) 的增加的铁内容在 PD 病人,和二价的金属 transporter 被发现了(DMT1 ) 1 被显示了在两个导致 MPTP 的 PD 模型和 PD 病人的 SN 起来调整。然而,位于 DMT1 起来规定下面的机制大部分是未知的。在现在的学习,我们观察到那在 6-hydroxydopamine ( 6-OHDA )的 SN 导致了 PD 老鼠,有铁的 DMT1 应答的元素(怒火, DMT1+IRE ),然而并非没有怒火( DMT1?IRE )的 DMT1 ,是起来调整的,建议那增加的 DMT1+IRE 表情可能在 PD 老鼠说明 nigral 铁累积。这可能性进一步被估计在一在里面 vitro 学习使用 6-OHDA-treated 和 DMT1+IRE-over-expressing MES23.5 房间。在 6-OHDA-treated MES23.5 房间,增加了铁规章的蛋白质(IRP ) 1 并且 IRP2 表示被观察,当 IRP 的 silencing 戏剧性地减少了时 6-OHDA-induced DMT1+IRE 起来规定。有 N-acetyl-L-cysteine 的预告的处理充分由 6-OHDA-induced 的抑制压制了 IRP 起来规定氧化应力。增加的 DMT1+IRE 表示由 MES23.5 房间导致了增加的铁流入。我们的数据提供 DMT1+IRE 起来规定能说明细胞内部的氧化应力和那增加了的 6-OHDA-induced 开始的 IRE/IRP-dependent 6-OHDA-induced 铁累积的直接证据细胞内部的铁的层次导致加重的氧化应力。这研究的结果提供在 PD 的处理支持抗氧化剂的使用的新奇证据,旨在禁止由 DMT1 表示的规定的铁累积。 | Hong Jiang Ning Song Huamin Xu Shuzhen Zhang Jun Wang Junxia Xie | 2010 | Cell Research2010，20，3： | 25 |
| 7 | Curative effect of heat-sensitive moxibustion on chronic persistent asthma: a multicenter randomized controlled trial显示文摘OBJECTIVE:To compare the curative effects of heat-sensitive moxibustion with conventional drugs on chronic persistent asthma and seek a valuable therapy to replaceWestern Medicine.METHODS:The participants in this multi-center,randomized,and controlled study were randomly divided into two groups:group A(n=144),treated with heat-sensitive moxibustion(50 sessions)and group B(n=144),treated with Seretide(salmeterol50μg/fluticasone 250μg,twice a day).The scores of asthma control test(ACT),forced expiratory volume in 1 second(FEV1),peak expiratory flow(PEF),and attack frequency were measured after 15,30,60,and 90 days of treatment.Patients followed up3 and 6 months after treatment.RESULTS:There was a significant difference(P=0.0002)in the ACT score and lung function between the two groups after 3 months of treatment and(P=0.000 03)during the follow-up visits.In addition,heat-sensitive moxibustion reduced attack frequency in the period from inclusion to the6-month follow-up visit.CONCLUSION:This study shows that heat-sensitive moxibustion may have a comparable curative effect toSeretide(salmeterol/fluticasone)on asthma. | Rixin Chen Mingren Chen Jun Xiong Zhenhai Chi B0 Zhang Ning Tian Zhenhua Xu Tangfa Zhang Wanyao Li Wei Zhang Xiaofeng Rong Zhen Wang Gang Sun Baohe Ge Guoxiong Yu Nanchang Song | 2013 | Journal of Traditional Chinese Medicine2013，33，5： | 20 |
| 8 | Association of sterol regulatory element binding protein 2 and insulin-like growth factor binding protein 3 genetic polymorphisms with avascular necrosis of the femoral head in the Chinese population显示文摘 | SONG Yang DU Zhen-wu LI Qiu-ju ZHANG Gui-zhen WANG Ling-ling WU Ning WANG Jin-cheng GAO Zhong-li | 2012 | Chinese Medical Journal2012，，22： | 19 |
| 9 | Primary Ewing's sarcoma/primitive neuroectodermal tumor of the urogenital tract in children显示文摘背景主要尤因尿生殖的道的肉瘤/原语 neuroectodermal 肿瘤( ES/PNET )是有非特定的临床的演讲的一个稀罕条件,它能使它困难到 diagnose.In 这研究,我们总结临床的演讲,病理学的特征,治疗学的策略,并且在阴茎和输尿管的 ES/PNET 的盒子被分析的二的 ES/PNET.Methods 临床的信息的预后,和相关文学是 reviewed.Results ES/PNET 病理地被证实, | SONG Hong-cheng SUN Ning ZHANG Wei-ping HUANG Cheng-ru | 2012 | Chinese Medical Journal2012，，5： | 18 |
| 10 | Hot deformation behavior and constitutive model of TC18 alloy during compression显示文摘The hot deformation behavior of TC18 alloy at strain rates ranging from 1 9 10-4to 1 9 10-2s-1and temperatures ranging from 25 to 800 °C was studied using a WDW-300 electronic universal testing machine. The relationships between true stress and true strain show that flow stress decreases with the increase of temperature and increases as strain rate increases. The effect of strain rate on the flow stress becomes pronounced at higher temperatures. At room temperature, the river pattern characteristic of brittle fracture and the dimple pattern typical of ductile fracture are found to exist in different regions of fracture surfaces of the samples. An improved constitutive relationship is proposed to accurately describe the flow stress of TC18 by considering the effect of strain. And a microscopic model is also deduced which can link the physical mechanisms to the macroscopic experimental results. A good agreement is obtained between the predictions of the microscopic model and the results of the macroscopic experiment. | Bao-Hua Jia Wei-Dong Song Hui-Ping Tang Zhi-Hua Wang Xiao-Nan Mao Jian-Guo Ning | 2014 | Rare Metals2014，33，4： | 16 |
| 11 | COVID-19 Outbreak Caused by Contaminated Packaging of Imported Cold-Chain Products—Liaoning Province,China,July 2020显示文摘Summary What is known about this topic?Few major outbreaks of coronavirus disease 2019(COVID-19)have occurred in China after major nonpharmaceutical interventions and vaccines have been deployed and implemented.However,sporadic outbreaks that had high possibility to be linked to cold chain products were reported in several cities of China..What is added by this report?In July 2020,a COVID-19 outbreak occurred in Dalian,China.The investigations of this outbreak strongly suggested that the infection source was from COVID-19 virus-contaminated packaging of frozen seafood during inbound unloading personnel contact.What are the implications for public health practice?Virus contaminated paper surfaces could maintain infectivity for at least 17–24 days at-25℃.Exposure to COVID-19 virus-contaminated surfaces is a potential route for introducing the virus to a susceptible population.Countries with no domestic transmission of COVID-19 should consider introducing prevention strategies for both inbound travellers and imported goods.Several measures to prevent the introduction of the virus via cold-chain goods can be implemented. | Huilai Ma1 Jianqun Zhang Ji Wang Ying Qin Cao Chen Yang Song Liang Wang Jun Meng Lingling Mao Fengqin Li Ning Li Jian Cai Yong Zhang Dayan Wang Yunting Xia Hong Wang Shaofeng Jiang Xiang Zhao Peihua Niu Wenjie Tan Tao Ma Yecheng Yao Naiying Mao Zhen Zhu Tianjiao Ji Qian Yang Baoying Huang Li Zhao Jianxing Yu Li Bai Shuangli Zhu Dongyan Wang Yan Zhang Yingwei Sun Mingchun Luan Yanhai Wang Haibo Sun Shihong Yang Zhijian Bo Xiang Ren Zhongjie Li George Fu Gao Wei Yao Wenqing Yao Zijian Feng Wenbo Xu | 2021 | China CDC weekly2021，3，21： | 16 |
| 12 | Petroleum hydrocarbons and their effects on fishery species in the Bohai Sea, North China显示文摘Systematic studies on the changes in concentrations of petroleum hydrocarbons (PHs) and their effects on fishery species in the Bohai Sea during 1974-2004 are presented. Changes in PHs concentrations were closely related to Yellow River runoff. Concentrations of PHs accumulated in fish and shrimp increased by about 0.712 mg/kg dry weight when trophic level of fish and shrimps increased by 1. Attention should also be paid to the high PHs concentrations in mollusks along the coastal waters of the Bohai Sea. Average concentration of PHs in the adjacent coastal waters of Tianjin City during 1996-2005 decreased the population growth rates of fish, crustaceans and mollusks in the Bohai Sea by 2.58%, 6.59% and 2.33%, respectively. Therefore, PHs have significantly contributed to the decline in fisheries in the Bohai Sea, and they must be reduced to realize the sustainable fisheries. | Sisi Xu Jinming Song Huamao Yuan Xuegang Li Ning Li Liqin Duan Yu Yu | 2011 | Journal of Environmental Sciences2011，23，4： | 15 |
| 13 | Organic carbon source and burial during the past one hundred years in Jiaozhou Bay,North China显示文摘器官的碳(OC ) ,总氮(TN ) ,和在核心沉积的 210Pb 被测量确定器官的碳和属曾被地壳动力搬、本土的贡献的相对重要性的埋葬在在 Jiaozhou 海湾的过去的 100 年期间,诺思中国。核心沉积用 210Pb 年表被标明日期,它是最有希望的方法因为在一时间的沉积率的评价 100-150 年可伸缩。过去的 100 年里的器官的碳的埋葬流动的变化能被划分成下列三个阶段:( 1 )以前相对稳定1980年代;( 2 )在1990年代从1980年代很快增加到一座山峰,并且( 3 )从1990年代减少到礼品。变化与倒进海湾的稳固的废物和污水的数量一致。OC : TN 比率被用来在 Jiaozhou 海湾沉积评估器官的碳的来源。在内部海湾和海湾嘴,器官的碳是从陆上的来源的主要贡献者,而在外部海湾从陆上的来源仅仅关于器官的碳的一半被贡献。在内部海湾,器官的碳的陆上的来源在 2000 在 1990 ~ 93% 前随 69%-77% 的范围的增加显示出一个稳定的变化,然后因为减少,在陆上的输入从 2000 减少了。在海湾嘴,从土地的器官的碳的百分比在 1994 与 94% 到达了最高的价值。在外部海湾,沉积来源维持了为过去的 100 年稳定。 | LI Xuegang YUAN Huamao LI Ning SONG Jinming | 2008 | Journal of Environmental Sciences2008，20，5： | 15 |
| 14 | Current Status of Diagnosis And Treatment of Primary Breast Cancerin Beijing,2008显示文摘在北京调查诊断的地位和主要乳癌的治疗的目的, 2008。在 2008 在北京作为主要乳癌被诊断的所有病人在这被注册的方法学习。这些病人的信息包括肿瘤的特征,临床的诊断和治疗被收集,并且填设计得好的问询表表格 by trained 测量员。失踪的数据部分通过电话会见被补充。3473 个北京公民全部的结果 A 在北京作为主要乳癌(有同时的双边的乳癌的 25 个病人) 被诊断, 2008。他们中, 82.09% 是征兆的。19.02% 和 34.11% 分别地用好针渴望活体检视(FNAB ) 和核心针活体检视(CNB ) 被诊断。15.92% 收到的哨兵淋巴节点活体检视(SLNB ) 和 24.27% 收到的胸保存外科(英国计算机学会) 。在有 Her-2 的 476 个案例之中积极, 96 仅仅收到的 anti-Her-2 治疗。我们发现标准化水平在不同等级的医院里变化了,与 Grade-III 医院里的高水平。笔记,一些乳癌病人收到了非标准的主要肿瘤治疗:有在 situ (DCIS ) 的 ductal 癌的 65.63% 病人收到了腋的淋巴节点解剖和 36.88% 收到的化疗;25.89% 病人经历了没有边缘地位,保存外科的胸;12.10% 病人收到了化疗不到 4 个周期。结论尽管大多数乳癌病人收到了基本医疗保健,诊断和治疗的模式应该被改进并且应该在北京在未来被标准化。 | Xiao-mei Yuan Ning Wang Tao OuYang Lei Yang Ming-yang Song Ben-yao Lin Yun-tao Xie Jin-feng Li Kai-feng Pan Wei-cheng You Lian Zhang | 2011 | Chinese Journal of Cancer Research2011，23，1： | 14 |
| 15 | Efficacy of totally laparoscopic compared with laparoscopic-assisted total gastrectomy for gastric cancer: A meta-analysis显示文摘BACKGROUND Laparoscopic radical gastrectomy is currently the most common surgical approach for gastric cancer.The main difference between totally laparoscopic total gastrectomy(TLTG)and laparoscopic-assisted total gastrectomy(LATG)is the route of digestive tract reconstruction.However,TLTG is currently not widespread as the safety and feasibility of intracorporeal esophagojejunostomy is uncertain.AIM To compare the short-term efficacy of TLTG and LATG for radical gastrectomy of gastric cancer,and to determine the safety and feasibility of intracorporeal esophagojejunostomy.METHODS PubMed,EMBASE,and Web of Science databases were searched for all relevant articles regarding TLTG vs LATG for gastric cancer published up to October 1,2019.Inclusion and exclusion criteria were established.All the basic conditions of patients and important clinical data related to surgery were extracted,and a meta-analysis was performed with RevMan 5.3 software.RESULTS Eight studies involving a total of 1883 cases(869 cases in the TLTG group and 1014 cases in the LATG group)were included.Compared with the LATG group,reduced intraoperative blood loss(weighted mean difference=-35.37,95%CI:-61.69--9.06,P=0.008)and a larger number of retrieved lymph nodes(weighted mean difference=3.11,95%CI:-2.60-12.00,P=0.01)were found in the TLTG group.There were no significant differences in operating time,anastomotic time,tumor size,proximal resection margin length,postoperative pain score,time to first flatus,time to first oral intake,postoperative hospital stay,postoperative anastomosis-related complication rate and overall complication rate between the two groups(P>0.05).CONCLUSION Intracorporeal esophagojejunostomy is safe and feasible.TLTG has the advantages of being minimally invasive,reduced intraoperative blood loss and easier access to lymph nodes compared with LATG.Totally laparoscopic gastrectomy is likely to be the surgical trend for gastric cancer in the future. | Song Wang Mei-Lan Su Yang Liu Zhi-Ping Huang Ning Guo Tian-Jin Chen Zhong-Hui Zou | 2020 | World Journal of Clinical Cases2020，8，5： | 14 |
| 16 | Application of next-generation sequencing technology to precision medicine in cancer: joint consensus of the Tumor Biomarker Committee of the Chinese Society of Clinical Oncology显示文摘Next-generation sequencing(NGS) technology is capable of sequencing millions or billions of DNA molecules simultaneously.Therefore, it represents a promising tool for the analysis of molecular targets for the initial diagnosis of disease, monitoring of disease progression, and identifying the mechanism of drug resistance. On behalf of the Tumor Biomarker Committee of the Chinese Society of Clinical Oncology(CSCO) and the China Actionable Genome Consortium(CAGC), the present expert group hereby proposes advisory guidelines on clinical applications of NGS technology for the analysis of cancer driver genes for precision cancer therapy. This group comprises an assembly of laboratory cancer geneticists, clinical oncologists, bioinformaticians,pathologists, and other professionals. After multiple rounds of discussions and revisions, the expert group has reached a preliminary consensus on the need of NGS in clinical diagnosis, its regulation, and compliance standards in clinical sample collection. Moreover, it has prepared NGS criteria, the sequencing standard operation procedure(SOP), data analysis, report, and NGS platform certification and validation. | Xuchao Zhang Zhiyong Liang Shengyue Wang Shun Lu Yong Song Ying Cheng Jianming Ying Weiping Liu Yingyong Hou Yangqiu Li Yi Liu Jun Hou Xiufeng Liu Jianyong Shao Yanhong Tai Zheng Wang Li Fu Hui Li Xiaojun Zhou Hua Bai Mengzhao Wang You Lu Jinji Yang Wenzhao Zhong Qing Zhou Xuening Yang Jie Wang Cheng Huang Xiaoqing Liu Xiaoyan Zhou Shirong Zhang Hongxia Tian Yu Chen Ruibao Ren Ning Liao Chunyan Wu Zhongzheng Zhu Hongming Pan Yanhong Gu Liwei Wang Yunpeng Liu Suzhan Zhang Tianshu Liu Gong Chen Zhimin Shao Binghe Xu Qingyuan Zhang Ruihua Xu Lin Shen Yilong Wu | 2019 | Cancer Biology & Medicine2019，16，1： | 14 |
| 17 | Source of major anions and cations of snowpacks in Hailuogou No.1 glacier, Mt. Gongga and Baishui No.1 glacier, Mt. Yulong显示文摘积雪场样品是从二条冰河的 colleted:在 2006 年 6 月的 Baishui No.1 冰河和 Hailuogou No.1 冰河。海盐离子 tracer。 | LI Zongxing HE Yuanqing PANG Hongxi YANG Xiaomei JIA Wenxiong ZHANG Ningning WANG Xufeng NING Baoying YUAN Lingling SONG Bo | 2008 | Journal of Geographical Sciences2008，18，1： | 13 |
| 18 | HBx-DNA probe preparation and its application in study of hepatocarcinogenesis显示文摘HBxDNAprobepreparationanditsapplicationinstudyofhepatocarcinogenesisGAOFengGuang,SUNWenSheng,CAOYingLin,ZHANGLiNing,SON... | GAO FengGuang, SUN WenSheng, CAO YingLin, ZHANG LiNing, SONG Jing, LI HuaFen and YAN ShiKunKeywords hepatitis B virus liver neoplasms/etiology carcinoma, hepatocellular/etiology DNA probe | 1998 | World Journal of Gastroenterology1998，4，4： | 12 |
| 19 | Efficiency and Inheritance of Targeted Mutagenesis in Maize Using CRISPR-Cas9显示文摘CRISPR/Cas(clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins) is an adaptive immune system in bacteria and archaea to defend against invasion from foreign DNA fragments. Recently, it has been developed as a powerful targeted genome editing tool for a wide variety of species. However, its application in maize has only been tested with transiently expressed somatic cells or with a limited number of stable transgenic T0 plants. The exact efficiency and specificity of the CRISPR/Cas system in the highly complex maize genome has not been documented yet. Here we report an extensive study of the well-studied type II CRISPR-Cas9 system for targeted genome editing in maize, with the codon-optimized Cas9 protein and the short non-coding guide RNA generated through a functional maize U6 sn RNA promoter. Targeted gene mutagenesis was detected for 90 loci by maize protoplast assay, with an average cleavage efficiency of 10.67%. Stable knockout transformants for maize phytoene synthase gene(PSY1) were obtained. Mutations occurred in germ cells can be stably inherited to the next generation. Moreover, no off-target effect was detected at the computationally predicted putative off-target loci. No significant difference between the transcriptomes of the Cas9 expressed and non-expressed lines was detected. Our results confirmed that the CRISPR-Cas9 could be successfully applied as a robust targeted genome editing system in maize. | Jinjie Zhu Ning Song Silong Sun Weilong Yang Haiming Zhao Weibin Song Jinsheng Lai | 2016 | Journal of Genetics and Genomics2016，43，1： | 12 |
| 20 | Low-dose metformin treatment in the subacute phase improves the locomotor function of a mouse model of spinal cord injury显示文摘Metformin,a first-line drug for type-2 diabetes,has been shown to improve locomotor recovery after spinal cord injury.However,there are studies reporting no beneficial effect.Recently,we found that high dose of metformin(200 mg/kg,intraperitoneal)and acute phase administration(immediately after injury)led to increased mortality and limited locomotor function recovery.Consequently,we used a lower dose(100 mg/kg,i.p.)metformin in mice,and compared the effect of immediate administration after spinal cord injury(acute phase)with that of administration at 3 days post-injury(subacute phase).Our data showed that metformin treatment starting at the subacute phase significantly improved mouse locomotor function evaluated by Basso Mouse Scale(BMS)scoring.Immunohistochemical studies also revealed significant inhibitions of microglia/macrophage activation and astrogliosis at the lesion site.Furthermore,metformin treatment at the subacute phase reduced neutrophil infiltration.These changes were in parallel with the increased survival rate of spinal neurons in animals treated with metformin.These findings suggest that low-dose metformin treatment for subacute spinal cord injury can effectively improve the functional recovery possibly through anti-inflammation and neuroprotection.This study was approved by the Institute Animal Care and Use Committee at the University of Texas Medical Branch(approval No.1008041C)in 2010. | Wen-Ye Song Han Ding Tiffany Dunn Jun-Ling Gao Javier Allende Labastida Caitlin Schlagal Guang-Zhi Ning Shi-Qing Feng Ping Wu | 2021 | Neural Regeneration Research2021，16，11： | 12 |
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