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| 1 | Ginkgolide B promotes the proliferation and differentiation of neural stem cells following cerebral ischemia/reperfusion injury,both in vivo and in vitro显示文摘Neural stem cells have great potential for the development of novel therapies for nervous system diseases.However,the proliferation of endogenous neural stem cells following brain ischemia is insufficient for central nervous system self-repair.Ginkgolide B has a robust neuroprotective effect.In this study,we investigated the cell and molecular mechanisms underlying the neuroprotective effect of ginkgolide B on focal cerebral ischemia/reperfusion injury in vitro and in vivo.Neural stem cells were treated with 20,40 and 60 mg/L ginkgolide B in vitro.Immunofluorescence staining was used to assess cellular expression of neuron-specific enolase,glial fibrillary acid protein and suppressor of cytokine signaling 2.After treatment with 40 and 60 mg/L ginkgolide B,cells were large,with long processes.Moreover,the proportions of neuron-specific enolase-,glial fibrillary acid protein-and suppressor of cytokine signaling 2-positive cells increased.A rat model of cerebral ischemia/reperfusion injury was established by middle cerebral artery occlusion.Six hours after ischemia,ginkgolide B(20 mg/kg) was intraperitoneally injected,once a day.Zea Longa's method was used to assess neurological function.Immunohistochemistry was performed to evaluate the proportion of nestin-,neuron-specific enolase-and glial fibrillary acid protein-positive cells.Real-time quantitative polymerase chain reaction was used to measure m RNA expression of brain-derived neurotrophic factor and epidermal growth factor.Western blot assay was used to analyze the expression levels of brain-derived neurotrophic factor and suppressor of cytokine signaling 2.Ginkgolide B decreased the neurological deficit score,increased the proportion of nestin-,neuron-specific enolase-and glial fibrillary acid protein-positive cells,increased the m RNA expression of brain-derived neurotrophic factor and epidermal growth factor,and increased the expression levels of brain-derived neurotrophic factor and suppressor of cytokine signaling 2 in the ischemic penumbra.Together,the in vivo and in vitro findings suggest that ginkgolide B improves neurological function by promoting the proliferation and differentiation of neural stem cells in rats with cerebral ischemia/reperfusion injury. | Pei-Dong Zheng Rajneesh Mungur Heng-Jun Zhou Muhammad Hassan Sheng-Nan Jiang Jie-Sheng Zheng | 2018 | Neural Regeneration Research2018，13，7： | 21 |
| 2 | 早期常压高氧治疗对大鼠颅脑损伤后线粒体功能及细胞凋亡的影响显示文摘目的探讨早期常压高氧治疗对大鼠颅脑损伤后线粒体功能及细胞凋亡的保护作用及其机制。方法将成年健康雄性大鼠分为假手术组、模型组、高压氧组及常压氧组,每组28只。于伤后1周行神经功能缺损评分,并检测线粒体膜电位(MMP)、丙二醛(MDA)和超氧化物歧化酶(SOD)的变化。观察各组电镜下线粒体形态,并采用原位缺口末端标记(TUNEL)法检测神经细胞凋亡情况。结果四组间神经功能缺损评分比较差异有统计学意义(F=3.682,P=0.039),且常压氧组与高压氧组较模型均明显改善(P均<0.05)。四组间MMP、MDA、SOD及阳性细胞计数比较,差异均有统计学意义(F=8.571、12.4、6.604、9.425,P均<0.05),与模型组比较,常压氧组及高压氧组的MMP均明显升高,MDA、SOD及阳性细胞计数均明显下降(P均<0.05),而高压氧组与常压氧组的MMP、MDA、SOD及阳性细胞计数比较,差异均无统计学意义(P均>0.05)。电镜下,模型组线粒体膜出现断裂、形态较大、肿胀明显,线粒体内出现空泡化,线粒体嵴消失;而常压氧及高压氧组线粒体膜基本完整,肿胀程度较模型组轻微,线粒体嵴尚存在,未见明显空泡化现象,且后两者线粒体形态差异不明显。结论早期常压氧治疗通过保护线粒体结构和功能从而抑制缺血半影区的细胞凋亡,以促进大鼠脑外伤后的神经功能的恢复。 | 朱岁军 刘晨 王俊 唐超 单华 李伟 Rajneesh Mungur 沈建 | 2015 | 中华危重症医学杂志(电子版)2015，8，1： | 6 |
| 3 | Bifunctional yttrium( Ⅲ ) and titanium ( Ⅳ ) NHC catalysts for lactide polymerisation 显示文摘 | Patel D Liddle S T Mungur S A | 2006 | Chem Commun2006，，10： | 1 |
| 4 | The glutamate dehydrogenase gene gdh A increased the resistance of tobacco to glufosinate显示文摘 | Nolte S A Young B G Mungur R | 2004 | Weed Res2004，44，： | 1 |
| 5 | An assessment of metal removal by a laboratory scale wetland显示文摘 | Mungur A S Shutest R B E Revirt D M | 1997 | Water Science and Technology1997，35，5： | 1 |
| 6 | An assessment of metal removal by a laboratory scale wetland显示文摘 | Mungur A S Shutest R B E Revirt D M | 1997 | Water Science and Technology1997，35，5： | 1 |
| 7 | The Nematode Resistance Allele at the rhg1 Locus Alters the Proteome and Primary Metabolism of Soybean Roots1[W][OA]显示文摘 | Afzal Ahmed J Natarajan Aparna Saini Navinder Iqbal M Javed Geisler Matt El Shemy Hany A Mungur Rajsree Willmitzer Lothar Lightfoot David A | 2009 | Plant Physiology2009，，3： | 1 |
| 8 | Adipose tissue-derived stem ceils attenuate acute lung injury through eNOS and eNOS-derived NO 显示文摘 | Gao P Yang X Mungur L | 2013 | IntJ Mol Med2013，31，6： | 1 |
| 9 | An assessment of metal removal by a laboratory scale wetland显示文摘 | Mungur A S Shutes R B E Revitt D M | 1997 | Water Science andTechnology1997，35，5： | 1 |
| 10 | An assessment of metal removal by a laboratory scale wetland 显示文摘 | MUNGUR A S SHUTES R B E REVITT D M | 1997 | Water Science and Technology1997，35，5： | 1 |
| 11 | Adipose tissue-derived stem ceils attenuate acute lung injury through eNOS and eNOS-derived NO显示文摘 | Gao P Yang X Mungur L | 2013 | Int J Mol Med2013，31，6： | 1 |
| 12 | The glutamate dehydrogenase gene gdhA increased the resistance of tobacco to glufosinate显示文摘 | NOLTE S A YOUNG B G MUNGUR R | 2004 | Weed Res2004，44，4： | 1 |
| 13 | The design of wetland systems for the treatment of urban run off显示文摘 | Shutes R B E Revitt D M Mungur A S | 1997 | Water Sci Technol1997，35，5： | 1 |
| 14 | Assessment of metal removal by a laboratory scale wetland显示文摘 | Mungur A S Shutes R B E Revitt D M | 1997 | Water Science and Technology1997，35，5： | 1 |
| 15 | A wireless sen- sor network air pollution monitoring system 显示文摘 | Khedo K K Perseedoss R Mungur A | 2010 | Interna- tional Journal of Wireless & Mobile Networks2010，2，2： | 1 |
| 16 | An Assessment of metal removal by a laboratory scale wetland显示文摘 | Mungur A S Shutes R B E Revitt D M | 1997 | Wat Sci Tech1997，35，5： | 1 |
| 17 | An assessment of metal removal by a laboratory scale wetland 显示文摘 | MUNGUR A S SHUTEST R B E REVIRT D M | 1997 | Water Science and Technology1997，35，5： | 1 |
| 18 | An assessment of metal removal by laboratory scale wetland显示文摘 | Mungur A S Shutes R B E Revitt D M | 1997 | Water Science Technology1997，35，5： | 1 |
| 19 | An assessment of metal removal by a laboratory scale wetland显示文摘 | Mungur A S Shutest R B E Revirt D M et a1 | 1997 | Water Science and Technology1997，35，5： | 1 |
| 20 | A Wireless Sensor Network Air Pollution Monitoring System显示文摘 | Khedo K K Perseedoss R Mungur A | 2010 | International Journal of Wireless & Mobile Networks2010，2，2： | 1 |
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