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您的检索式：作者名="Muhammad Junaid Rao"
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| 1 | Genome of a citrus rootstock and global DNA demethylation caused by heterografting显示文摘Grafting is an ancient technique used for plant propagation and improvement in horticultural crops for at least 1,500 years.Citrus plants,with a seed-to-seed cycle of 5–15 years,are among the fruit crops that were probably domesticated by grafting.Poncirus trifoliata,a widely used citrus rootstock,can promote early flowering,strengthen stress tolerance,and improve fruit quality via scion–rootstock interactions.Here,we report its genome assembly using PacBio sequencing.We obtained a final genome of 303 Mb with a contig N50 size of 1.17Mb and annotated 25,680 protein-coding genes.DNA methylome and transcriptome analyses indicated that the strong adaptability of P.trifoliata is likely attributable to its special epigenetic modification and expression pattern of resistance-related genes.Heterografting by using sweet orange as scion and P.trifoliata as rootstock and autografting using sweet orange as both scion and rootstock were performed to investigate the genetic effects of the rootstock.Single-base methylome analysis indicated that P.trifoliata as a rootstock caused DNA demethylation and a reduction in 24-nt small RNAs(sRNAs)in scions compared to the level observed with autografting,implying the involvement of sRNA-mediated graft-transmissible epigenetic modifications in citrus grafting.Taken together,the assembled genome for the citrus rootstock and the analysis of graft-induced epigenetic modifications provide global insights into the genetic effects of rootstock–scion interactions and grafting biology. | Yue Huang Yuantao Xu Xiaolin Jiang Huiwen Yu Huihui Jia Chunming Tan Gang Hu Yibo Hu Muhammad Junaid Rao Xiuxin Deng Qiang Xu | 2021 | Horticulture Research2021，8，1： | 4 |
| 2 | Genomic insights into citrus domestication and its important agronomic traits显示文摘Citrus originated in Southeast Asia,and it has become one of the most important fruit crops worldwide.Citrus has a long and obscure domestication history due to its clonal propagation,long life cycle,wide sexual compatibility,and complex genetic background.As the genomic information of both wild and cultivated citrus becomes available,their domestication history and underlying traits or genes are becoming clear.This review outlines the genomic features of wild and cultivated species.We propose that the reduction of citric acid is a critical trait for citrus domestication.The genetic model representing the change during domestication may be associated with a regulatory complex known as WD-repeat-MYB-bHLH-WRKY(WMBW),which is involved in acidification and anthocyanin accumulation.The reduction in or loss of anthocyanins may be due to a hitchhiking effect of fruit acidity selection,in which mutation occurs in the common regulator of these two pathways in some domesticated types.Moreover,we have summarized the domestication traits and candidate genes for breeding purposes.This review represents a comprehensive summary of the genes controlling key traits of interest,such as acidity,metabolism,and disease resistance.It also sheds light on recent advances in early flowering from transgenic studies and provides a new perspective for fast breeding of citrus.Our review lays a foundation for future research on fruit acidity,flavor,and disease resistance in citrus. | Muhammad Junaid Rao Hao Zuo Qiang Xu | 2021 | Plant Communications2021，2，1： | 0 |
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