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您的检索式：作者名="Michail Michailidis"
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| 1 | Decoding altitude-activated regulatory mechanisms occurring during apple peel ripening显示文摘Apple(Malus domestica Borkh)is an important fruit crop cultivated in a broad range of environmental conditions.Apple fruit ripening is a physiological process,whose molecular regulatory network response to different environments is still not sufficiently investigated and this is particularly true of the peel tissue.In this study,the influence of environmental conditions associated with low(20 m)and high(750 m)altitude on peel tissue ripening was assessed by physiological measurements combined with metabolomic and proteomic analyses during apple fruit development and ripening.Although apple fruit ripening was itself not affected by the different environmental conditions,several key color parameters,such as redness and color index,were notably induced by high altitude.Consistent with this observation,increased levels of anthocyanin and other phenolic compounds,including cyanidin-3-O-galactoside,quercetin-3-O-rhamnoside,quercetin-3-O-rutinoside,and chlorogenic acid were identified in the peel of apple grown at high altitude.Moreover,the high-altitude environment was characterized by elevated abundance of various carbohydrates(e.g.,arabinose,xylose,and sucrose)but decreased levels of glutamic acid and several related proteins,such as glycine hydroxymethyltransferase and glutamate–glyoxylate aminotransferase.Other processes affected by high altitude were the TCA cycle,the synthesis of oxidative/defense enzymes,and the accumulation of photosynthetic proteins.From the obtained data we were able to construct a metabolite-protein network depicting the impact of altitude on peel ripening.The combined analyses presented here provide new insights into physiological processes linking apple peel ripening with the prevailing environmental conditions. | Evangelos Karagiannis Michail Michailidis Georgia Tanou Federico Scossa Eirini Sarrou George Stamatakis Martina Samiotaki Stefan Martens Alisdair R.Fernie Athanassios Molassiotis | 2020 | Horticulture Research2020，7，1： | 3 |
| 2 | Whole genome re-sequencing of sweet cherry(Prunus avium L.)yields insights into genomic diversity of a fruit species显示文摘Sweet cherries,Prunus avium L.(Rosaceae),are gaining importance due to their perenniallity and nutritional attributes beneficial for human health.Interestingly,sweet cherry cultivars exhibit a wide range of phenotypic diversity in important agronomic traits,such as flowering time and defense reactions against pathogens.In this study,wholegenome resequencing(WGRS)was employed to characterize genetic variation,population structure and allelic variants in a panel of 20 sweet cherry and one wild cherry genotypes,embodying the majority of cultivated Greek germplasm and a representative of a local wild cherry elite phenotype.The 21 genotypes were sequenced in an average depth of coverage of 33.91×.and effective mapping depth,to the genomic reference sequence of‘Satonishiki’cultivar,between 22.21×to 36.62×.Discriminant analysis of principal components(DAPC)with SNPs revealed two clusters of genotypes.There was a rapid linkage disequilibrium decay,as the majority of SNP pairs with r2 in near complete disequilibrium(>0.8)were found at physical distances less than 10 kb.Functional analysis of the variants showed that the genomic ratio of non-synonymous/synonymous(dN/dS)changes was 1.78.The higher dN frequency in the Greek cohort of sweet cherry could be the result of artificial selection pressure imposed by breeding,in combination with the vegetative propagation of domesticated cultivars through grafting.The majority of SNPs with high impact(e.g.,stop codon gaining,frameshift),were identified in genes involved in flowering time,dormancy and defense reactions against pathogens,providing promising resources for future breeding programs.Our study has established the foundation for further large scale characterization of sweet cherry germplasm,enabling breeders to incorporate diverse germplasm and allelic variants to fine tune flowering and maturity time and disease resistance in sweet cherry cultivars. | Aliki Xanthopoulou Maria Manioudaki Christos Bazakos Christos Kissoudis Anna-Maria Farsakoglou Evangelos Karagiannis Michail Michailidis Chrysanthi Polychroniadou Antonios Zambounis Konstantinos Kazantzis Athanasios Tsaftaris Panagiotis Madesis Filippos Aravanopoulos Athanassios Molassiotis Ioannis Ganopoulos | 2020 | Horticulture Research2020，7，1： | 0 |
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