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| 1 | 文献快报(15):父母监管及其与青少年危险性行为关系的Meta分析显示文摘美国15~24岁的青年人虽然只占全国有性经验人口的四分之一,但新发性传播疾病却有近一半发生在这一人群中尽管美国在减少少女妊娠方面取得了积极的进展,但其少女妊娠率仍是世界发达国家中最高的,每年约有750 000名20岁以下少女妊娠,其中有超过四分之三为非计划受孕。这些数据均表明青少年性与生殖健康仍是需要积极、持续关注的公共卫生问题。近年来,越来越多的保健服务提供者通过增强父母对青少年的监管来改善青少年性与生殖健康状况。 | DITTUS PJ MICHAEL SL BECASEN JS 苏普玉 | 2015 | 中国学校卫生2015，36，12： | 4 |
| 2 | Peridotite composition from theNorth Atlantic: regional and tectonic variations and implicationsfor partial melting显示文摘 | Michael PJ Bonatti E | 1985 | Earth and Planetary ScienceLetters1985，73，1： | 1 |
| 3 | Direct vascular and cardioprotective effects of rosuvastatin, a new HMG-CoA reductase inhibitor显示文摘 | Steven PJ Michael FG David MR | 2002 | JACC2002，40，6： | 1 |
| 4 | Chalenging the public stigma of mental illness:a meta-analysis of outcome studies 显示文摘 | Corrigan PW Morris SB Michaels PJ | 2012 | Psychiatric Services2012，63，10： | 1 |
| 5 | Ephrin A2 receptor targeting does not increase adenoviral pancreatic cancer transduction in vivo显示文摘AIM:To generate an adenoviral vector specifically targeting the EphA2 receptor(EphA2R) highly expressed on pancreatic cancer cells in vivo.METHODS:YSA,a small peptide ligand that binds the EphA2R with high affinity,was inserted into the HI loop of the adenovirus serotype 5 fiber knob.To further increase the specificity of this vector,binding sites for native adenoviral receptors,the coxsackie and adenovirus receptor(CAR) and integrin,were ablated from the viral capsid.The ablated retargeted adenoviral vector was produced on 293T cells.Specifi c targeting of this novel adenoviral vector to pancreatic cancer was investigated on established human pancreatic cancer cell lines.Upon demonstrating specifi c in vitro targeting,in vivo targeting to subcutaneous growing human pancreatic cancer was tested by intravenous and intraperitoneal administration of the ablated adenoviral vector.RESULTS:Ablation of native cellular binding sites reduced adenoviral transduction at least 100-fold.Insertion of the YSA peptide in the HI loop restored adenoviral transduction of EphA2R-expressing cells but not of cells lacking this receptor.YSA-mediated transduction was inhibited by addition of synthetic YSA peptide.The transduction specificity of the ablated retargeted vector towards human pancreatic cancer cells was enhanced almost 10-fold in vitro.In a subsequent in vivo study in a nude(nu/nu) mouse model however,no increased adenoviral targeting to subcutaneously growing human pancreas cancer nodules was seen upon injection into the tail vein,nor upon injection into the peritoneum.CONCLUSION:Targeting the EphA2 receptor increases specificity of adenoviral transduction of human pancreatic cancer cells in vitro but fails to enhance pancreatic cancer transduction in vivo. | Michael A van Geer Conny T Bakker Naoya Koizumi Hiroyuki Mizuguchi John G Wesseling Ronald PJ Oude Elferink Piter J Bosma | 2009 | World Journal of Gastroenterology2009，15，22： | 1 |
| 6 | Humoral rejection in cardiac transplantation: risk factors, hemodynamic consequences and relationship to transplant coronary artery disease 显示文摘 | Michaels PJ Espejo MJ Kobashigawa BJ | 2003 | J Heart Lung Transplant2003，22，1： | 1 |
| 7 | Functional Gastrointestinal Disorders:An Update for the Psychiatrist显示文摘 | Michael PJ Michael DC | 2007 | Psychosomatics2007，48，93： | 1 |
| 8 | Intraductal papillary mucinous neoplasm of the pancreas: cytologic features predict bis- tologic grade 显示文摘 | Michaels PJ Brachtel EF Bounds BC | 2006 | Cancer2006，108，3： | 1 |
| 9 | Coping strategies and interpersonal support in patients with irritable bowel syndrome and inflammatory bowel disease显示文摘 | Michael PJ Sarah W Michael D C | 2006 | Clinical Gastroenterology and Hepatology2006，4，4： | 1 |
| 10 | Coping st rategies and interpersonal support in patients with irritable bowel syndrome and inflammatory bowel disease 显示文摘 | Michael PJ Sarah W Michael DC | 2006 | Clinical Gast roenterology and Hepatology2006，4，4： | 1 |
| 11 | The water load test:observations fromhealthy controls and patients with functional dyspepsia显示文摘 | Michael PJ Seth Hoffman | 2003 | Am J physiol Gastrointest Liver physiol2003，284，： | 1 |
| 12 | NGF and GDNF am eliorate the increase in ATF3 expression which occurs in dorsal root ganglion cells in response to peripheral nerve injury显示文摘 | Averill S Michael GJ Shortland PJ | 2004 | Eur J Neurosci2004，19，： | 1 |
| 13 | Challenging the public stigma of mental illness; a recta-analysis of outcome studies显示文摘 | Corrigan PW Morris SB Michaels PJ | 2012 | Psychiatr Serv2012，63，10： | 1 |
| 14 | Intraductal papillary mucinous neoplasm of the pancreas:cytologic features predict histologic grade 显示文摘 | Michaels PJ Brachtel EF Bounds BC | 2006 | Cancer2006，108，3： | 1 |
| 15 | Pathophysiologicully relevant concentrations of tumor necrosis faetor-a promote progressive left ventricular dysfunction and remodeling in rats 显示文摘 | Bozkurt B Kribbs S Clubb FJ Jr Michael LH Didenko VV Homsby PJ Seta Y Oral H Spinale FG Mann DL | 1998 | Circulation1998，97，： | 1 |
| 16 | Intraductal pa-pillary mucinous neoplasm of the pancreas : cytologic features predicthistologic grade显示文摘 | MICHAELS PJ’BRACHTEL EF BOUNDS BC | 2006 | Cancer2006，108，3： | 1 |
| 17 | Intraductal papillary mutinous neoplasm of the pancreas:cytologic features predict histologic grade显示文摘 | Michaels PJ Brachtel EF Bounds BC | 2006 | Cancer2006，108，3： | 1 |
| 18 | Ex-vivo evaluation of gene therapy vectors in human pancreatic (cancer) tissue slices显示文摘AIM:To culture human pancreatic tissue obtained from small resection specimens as a pre-clinical model for examining virus-host interactions. METHODS:Human pancreatic tissue samples (malignant and normal)were obtained from surgical specimens and processed immediately to tissue slices. Tissue slices were cultured ex vivo for 1-6 d in an incubator using 95%O2.Slices were subsequently analyzed for viability and morphology.In addition the slices were incubated with different viral vectors expressing the reporter genes GFP or DsRed. Expression of these reporter genes was measured at 72 h after infection.RESULTS:With the Krumdieck tissue slicer,uniform slices could be generated from pancreatic tissue but only upon embedding the tissue in 3%low melting agarose.Immunohistological examination showed the presence of all pancreatic cell types.Pancreatic normal and cancer tissue slices could be cultured for up to 6 d,while retaining viability and a moderate to good morphology.Reporter gene expression indicated that the slices could be infected and transduced efficiently by adenoviral vectors and by adeno associated viral vectors,whereas transduction with lentiviral vectors was limited.For the adenoviral vector,the transduction seemed limited to the peripheral layers of the explants. CONCLUSION:The presented system allows reproducible processing of minimal amounts of pancreatic tissue into slices uniform in size,suitable for pre-clinical evaluation of gene therapy vectors. | Michael A van Geer Koert FD Kuhlmann Conny T Bakker Fibo JW ten Kate Ronald PJ Oude Elferink Piter J Bosma | 2009 | World Journal of Gastroenterology2009，15，11： | 1 |
| 19 | Ovarian 11β-Hydroxysteroid Dehydrogenase(11βHSD)Activity Is Suppressed in Women With Anovulatory Polycystic Ovary Syndrome(PCOS):Apparent Role for Ovarian Androgens显示文摘 | Michael AE Glenn C Wood PJ | 2013 | The Journal of Clinical Endocrinology&Metabolism2013，98，8： | 1 |
| 20 | Challenging the public stigma of mental illness: a meta-analysis of outcome studies显示文摘 | Corrigan PW Morris SB Michaels PJ | 2012 | Psychiatric Services2012，63，10： | 1 |
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