| 1 | The in-vivo assessment of Turkish propolis and its nano form on testicular damage induced by cisplatin显示文摘Objective: Chemotherapeutic drugs, such as cisplatin(CP), which are associated with oxidative stress and apoptosis, may adversely affect the reproductive system. This study tests whether administration of propolis and nano-propolis(NP) can alleviate oxidative stress and apoptosis in rats with testicular damage induced by CP.Methods: In this study, polymeric nanoparticles including propolis were synthesized with a green sonication method and characterized using Fourier transform-infrared spectroscopy, Brunauer-EmmettTeller, and wet scanning transmission electron microscopy techniques. In total, 56 rats were divided into the following seven groups: control, CP, propolis, NP-10, CP + propolis, CP + NP-10, and CP + NP-30.Propolis(100 mg/kg), NP-10(10 mg/kg), and NP-30(30 mg/kg) treatments were administered by gavage daily for 21 d, and CP(3 mg/kg) was administered intraperitoneally in a single dose. After the experiment,oxidative stress parameters, namely, malondialdehyde(MDA), glutathione(GSH), glutathione peroxidase(GPx), and catalase(CAT), and apoptotic pathways including B cell leukemia/lymphoma-2 protein(Bcl-2)and Bcl-2-associated X protein(Bax) were measured in testicular tissues. Furthermore, sperm quality and weights of the testis, epididymis, right cauda epididymis, seminal vesicles and prostate were evaluated.Results: Propolis and NP(especially NP-30) were able to preserve oxidative balance(decreased MDA levels and increased GSH, CAT, and GPx activities) and activate apoptotic pathways(decreased Bax and increased Bcl-2) in the testes of CP-treated rats. Sperm motility in the control, CP, and CP + NP-30 groups were 60%, 48.75%, and 78%, respectively(P<0.001). Especially, NP-30 application completely corrected the deterioration in sperm features induced by CP.Conclusion: The results show that propolis and NP treatments mitigated the side effects of CP on spermatogenic activity, antioxidant situation, and apoptosis in rats. | Pinar Tatli Seven Ismail Seven Selcan Karakus Seda Iflazoglu Mutlu Seyma Ozer Kaya Gozde Arkali Merve Ilgar Ezgi Tan Yesim Muge Sahin Deniz Ismik Ayben Kilislioglu | 2021 | Journal of Integrative Medicine2021，19，5： | 0 |
| 2 | Development of a functional chocolate using gamma-amino butyric acid producer Lacticaseibacillus rhamnosus NRRL B-442显示文摘The human microbiota is influenced by the immune and nervous systems of the host.Gamma-aminobutyric acid (GABA) is known as bioactive compound and it has important physiological functions,such as anti-hypertensive and antidepressant activities.Lactic acid bacteria (LAB),especially Lactobacillus species are known as the most important GABA producers because of the food-grade nature.The purpose of this study is to develop a functional chocolate using microencapsulated GABA producer Lacticaseibacillus rhamnosus NRRL B-442 strain for patients having an anxiety disorder.Water-in-oil emulsion technique was conducted for microencapsulation using whey-pullulan complex.Microencapsulated and free L.rhamnosus cell counts were 6.75 and 7.20 log CFU/g in chocolates,respectively,at the end of 60 days.During simulated in vitro digestion analysis,survival rate of microencapsulated bacteria in chocolate samples was found at higher percentage (87%) than free bacteria (75%).Furthermore,microencapsulated L.rhamnosus did not affect the physical,chemical,and sensory properties of chocolate.Consequently,L.rhamnosus with the highest GABA producing capability may provide insight for an anxiety disorder patient,since this strain has been thought as having a therapeutic effect.A new functional food model was developed for “GutBrain Axis” phenomena since the chocolate could be accepted as a good carrier for GABA producer bacteria. | Merve Ozer Burcu Ozturk Ali Adnan Hayaloglu Sebnem Tellioglu Harsa | 2022 | Food Bioscience2022，47，3： | 0 |
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