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1篇 您的检索式:作者名="Mengmin Hong"
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1First intron retention in part transcripts of OsEBP-89 gene in tissues of rice显示文摘OsEBP-89 is a transcription factor gene of rice. It contains two introns. Using RT-PCR and Southern hybridization to study OsEBP-89 tissue-specific expression, we found that its first intron (115 bp in length) of its was retained in a fraction of its transcripts of this gene in rice developing seeds. Furthermore, two OsEBP-89 cDNA clones (c89L and c89LH) were screened from a rice cDNA library. Sequence analysis revealed that the first intron was retained in c89L clone, whereas, both the first and second intron sequences were spliced in c89LH. In addition to developing seeds, the first intron unspliced transcripts of OsEBP-89 are detected in leaves and roots of rice, too. However, the ratio of the first intron unspliced to spliced OsEBP-89 transcripts varied in different tissues examined. The potential biological significance of intron retention in OsEBP-89 transcript was discussed.Huijun Yang Zongyang Wang Jingliu Zhang Xiuling Cai Mengmin Hong 2002Chinese Science Bulletin2002,47,5:0
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