| 1 | Assessment of germ cell apoptosis in cryptorchid rats显示文摘Aim:To investigate the relationship between germ cell edgeneration and apoptosis in cryptorchid rats.Methods:Thirteen21-day-oldWistar rats were made unilaterally cryptorchid by closing the left inguinal canal.At day30(Group1,n =6)and day60(Group2,n=7)after operation,the testes were removed for histopathological examination.30(Group1,n=6)and day60(Group2,n=7)after operation,the testes were removed for histopathological examination.The controls(n=8)were sham operated and were sacrificed at day60.Germ cell apoptosis was assessed by means of theTUNEL method.Results:Spermatogenesis was arrested and the testicular and seminiferous tubular diameters were significantly reduced In the Unilateral undescended testes(UUTs)compared with the contralateral descended testes(CDTs)and the control rats.However,atrophic changes,pathological calcification,necrosis of seminiferous tubule,and absence or sloughing of germ cells were not found in all the animals,The spermatocytes were the main type of germ cells undergoing apoptosis in all the groups.In the UUTs,there was a significant and time-dependent increase in the mean apoptotic index.By60days after surgery,increased apoptosis in gern cells was also observed in the CDTs.Conclusion:Apoptosis is the predominant mechanism of germ cell death rather than atrophy and necrosis in cryptorchidism. | IzzetKoak MehmetDündar Mine Hekimgil Pinar Okyay | 2002 | Asian Journal of Andrology2002，4，3： | 11 |
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