维普中文期刊产品整合服务
7篇 您的检索式:作者名="Maurice JM"
    题名 作者 年代 出处 被引量
1Relationships between the prefrontal cortex and the basal ganglia in the rat:physiology of the corticosubthalamic circuits显示文摘Maurice N Deniau JM Glowinski J 1998J Neurosci1998,18,22:1
2Hysteroscopic retrieval of a fractured intrauterine IUD显示文摘Burks AC Abbasy SA Maurice JM 2014J Minim Invasive Gyneco12014,21,6:1
3Prefrontal cortexbasal ganglia circuits in the rat:involvement of ventral pallidum and subthalamic nucleus显示文摘Maurice N Deniau JM Menetrey A 1998Synapse1998,29,4:1
4Quantitative trait loci underlying gene product variation:a novel perspective for analy- zing regulation of genome expression 显示文摘Damerval C Maurice A Josse JM 1994Genetics1994,137,1:1
5Relationships between the prefrontal cortex and the basal ganglia in the rat: physiology of the corticosubthalamic circuits 显示文摘Maurice N Deniau JM Glowinski J 1998J Neurosci1998,18,:1
6Short-course oral zidovudine for prevention of mother-to-child transmission of HIV-1 in Abidjan,Cote d'Ivoire:a randomised trial显示文摘Wiktor SZ Ekpini E Karon JM Nkengasong J Maurice C Severin ST 1999Lancet1999,353,:1
7Tuf m RNA rather than 16S r RNA is associated with culturable Staphylococcus aureus显示文摘AIM: To study the presence of various nucleic acids targets of Staphylococcus aureus(S. aureus) during bacterial growth and antibiotic induced killing in relation to viability.METHODS: S. aureus was cultured to log phase and spiked in Todd Hewitt(TH) broth and whole blood of healthy human volunteers. Viability of S. aureus after flucloxacillin treatment(0, 1, 3 and 6 d) was assessed by culture on bloodagar plates. DNA and RNA were isolated from 200 μL. c DNA synthesis was performed by using random primers. The presence of S. aureus DNA, r RNA, and m RNA were determined by real-time polymerase chain reaction of the 16 S r DNA and tuf gene(elongation factor Tu).RESULTS: S. aureus spiked in TH broth without antibiotics grew from day 0-6 and DNA(tuf and 16S), and 16 S r RNA remained detectable during this whole period. During flucloxacillin treatment S. aureus lost viability from day 3 onwards, while the 16 S r RNA-gene and its RNA transcripts remained detectable. DNA andr RNA can be detected in flucloxacillin treated S. aureus cultures that do not further contain culturable bacteria.However, tuf m RNA became undetectable from day 3onwards. Tuf m RNA can only be detected from samples with culturable bacteria. When spiking S. aureus in whole blood instead of broth no bacterial growth was seen, neither in the absence nor in the presence of flucloxacillin. Accordingly, no increase in DNA and RNA levels of both 16 S r DNA and the tuf gene were detected. CONCLUSION: Tuf m RNA expression is associated with culturable S. aureus and might be used to monitor antibiotic effects.Anne JM Loonen Petra FG Wolffs Maikel de Bresser Maurice Habraken Cathrien A Bruggeman Mirjam HA Hermans Adriaan JC van den Brule 2015World Journal of Clinical Infectious Diseases2015,5,4:0
返回顶部 每页显示:
共1页 首页 上一页 第1页 下一页 末页 /1 跳转

网站首页 | 关于我们 | 联系我们 | 产品服务 | 客服中心 | 广告服务 | 版权声明 | 网站联盟 | 友情链接 | 售卡网点

版权所有© 渝B2-20050021-1 渝公网安备 50019002500403号 违法和不良信息举报中心

互联网出版许可证 新出网证(渝)字10号 全国400电话 - 免长途话费