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| 1 | A comparative study of submicron particle sizing platforms: Accuracy, precision and resolution analysis of polydisperse particle size distributions显示文摘 | Will Anderson Darby Kozak Victoria A. Coleman ?sa K. J?mting Matt Trau | 2013 | Journal of Colloid And Interface Science2013，，： | 1 |
| 2 | DNA-directed assembly of copper nanoblocks with inbuilt fluorescent and electrochemical properties: Application in simultaneous amplification-free analysis of multiple RNA species显示文摘向金属性的离子的 DNA 分子的内在的亲密关系能以一种顺序依赖者方式在 nucleic 酸螺旋结构以内驾驶铜 nanostructures 的特定的形成。结果的 nanostructures 有趣荧光灯、电气化学性质,它为新奇 biosensing 应用吸引人。然而,为精确疾病诊断使用模板 DNA 的 nanostructures 的潜力仍然保持未经勘探。特别地,为不同 RNA biomarker 的通用没有扩大的察觉潜在的 DNAtemplated nanostructures 表演高度种类。因为他们的低细胞的层次和不同种类依赖者长度和顺序特征,不同送信人 RNA, microRNAs,和有一种单个技术的长非编码的 RNA 种类的同时的察觉是挑战性的。这里,我们报导一种当代的技术为在在基于杂交的磁性的隔离以后的各种各样的 RNA 种类目标上的模板 DNA 的铜 nanoblocks (CuNBs ) 的 situ 汇编灵巧。我们的途径围绕与扩大和当前的 RNA 试金的标记的过程联系的典型限制。综合 CuNBs 与灵活荧光或电气化学的读出启用了没有扩大的 fM 水平 RNA 察觉。而且,我们的 nanosensing 技术为临床的申请显示潜力,从 10 前列腺癌症病人的一个队由三诊断 RNA biomarkers 的非侵略的分析示威了有 100% 词语索引(量的反向的 transcriptionpolymerase 链反应(PCR ) 确认) 的尿样品。我们的方法的好分析性能和通用性可能在诊断和研究领域是有用的。 | Kevin M. Koo Laura G. Carrascosa Matt Trau | 2018 | Nano Research2018，11，2： | 1 |
| 3 | Polymeric grafting of acrylic acid onto poly(3-hydroxybutyrate-co-3-hydroxyvalerate):Surface functionalization for tissue engineering applications显示文摘 | Lisbeth Grφndahl Adrienne Chandler-Temple Matt Trau | 2005 | Biomacromolecules2005，6，： | 1 |
| 4 | Profiling proteomic responses to hexokinase-II depletion in terpene-producing Saccharomyces cerevisiae显示文摘Hexokinase II(Hxk2)is a master protein in glucose-mediated transcriptional repression signaling pathway.De-grading Hxk2 through an auxin-inducible protein degradation previously doubled sesquiterpene(nerolidol)pro-duction at gram-per-liter levels in Saccharomyces cerevisiae.Global transcriptomics/proteomics profiles in Hxk2-deficient background are important to understanding genetic and molecular mechanisms for improved nerolidol production and guiding further strain optimization.Here,proteomic responses to Hxk2 depletion are investi-gated in the yeast strains harboring a GAL promoters-controlled nerolidol synthetic pathway,at the exponential and ethanol growth phases and in GAL80-wildtype and gal80Δbackgrounds.Carbon metabolic pathways and amino acid metabolic pathways show diversified responses to Hxk2 depletion and growth on ethanol,including upregulation of alternative carbon catabolism and respiration as well as downregulation of amino acid synthesis.De-repression of GAL genes may contribute to improved nerolidol production in Hxk2-depleted strains.Seven-teen transcription factors associated with upregulated genes are enriched.Validating Ash1-mediated repression on the RIM4 promoter shows the variation on the regulatory effects of different Ash1-binding sites and the syner-gistic effect of Ash1 and Hxk2-mediated repression.Further validation of individual promoters shows that HXT1 promoter activities are glucose-dependent in hxk2Δbackground,but much weaker than those in HXK2-wildtype background.In summary,inactivating HXK2 may relieve glucose repression on respiration and GAL promoters for improved bioproduction under aerobic conditions in S.cerevisiae.The proteomics profiles provide a better genetics overview for a better metabolic engineering design in Hxk2-deficient backgrounds. | Zeyu Lu Qianyi Shen Lian Liu Gert Talbo Robert Speight Matt Trau Geoff Dumsday Christopher B.Howard Claudia E.Vickers Bingyin Peng | 2023 | Engineering Microbiology2023，3，3： | 0 |
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