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| 1 | Detection and characterization of murine colitis and carcinogenesis by molecularly targeted contrast-enhanced ultrasound显示文摘AIM To study mucosal addressin cellular adhesion molecule-1(MAd CAM-1) and vascular endothelial growth factor(VEGF)-targeted contrast enhanced ultrasound(CEUS) for the assessment of murine colitis and carcinogenesis. METHODS C57BL/6 mice were challenged with 3% dextran sodium-sulfate(DSS) for three, six or nine days to study the development of acute colitis. Ultrasound was performed with and without the addition of unspecific contrast agents. MAd CAM-1-targeted contrast agent was used to detect and quantify MAd CAM-1 expression. Inflammatory driven colorectal azoxymethane(AOM)/DSS-induced carcinogenesis was examined on day 42 and 84 using VEGF-targeted contrast agent. Highly specific tissue echogenicity was quantified using specialized software. Sonographic findings were correlated to tissue staining, western blot analysis and immunohistochemistry to quantify the degree of inflammation and stage of carcinogenesis. RESULTS Native ultrasound detected increased general bowel wall thickening that correlated with more progressed and more severe DSS-colitis(healthy mice: 0.3 mm ± 0.03 vs six days DSS: 0.5 mm ± 0.2 vs nine days DSS: 0.6 mm ± 0.2, P < 0.05). Moreover, these sonographic findings correlated well with clinical parameters such as weight loss(r2 = 0.74) and histological damage(r2 = 0.86)(P < 0.01). In acute DSS-induced murine colitis, CEUS targeted against MAd CAM-1 detected and differentiated stages of mild, moderate and severe colitis via calculation of mean pixel contrast intensity in decibel(9.6 d B ± 1.6 vs 12.9 d B ± 1.4 vs 18 d B ± 3.33, P < 0.05). Employing the AOM/DSSinduced carcinogenesis model, tumor development was monitored by CEUS targeted against VEGF and detected a significantly increased echogenicity in tumors as compared to adjacent healthy mucosa(healthy mucosa, 1.6 d B ± 1.4 vs 42 d, 18.2 d B ± 3.3 vs 84 d, 18.6 d B ± 4.9, P < 0.01). Tissue echogenicity strongly correlated with histological analysis and immunohistochemistry findings(VEGF-positive cells in 10 high power fields of healthy mucosa: 1 ± 1.2 vs 42 d after DSS start: 2.4 ± 1.6 vs 84 d after DSS start: 3.5 ± 1.3, P < 0.01). CONCLUSION Molecularly targeted CEUS is a highly specific and noninvasive imaging modality, which characterizes murine intestinal inflammation and carcinogenesis in vivo. | Markus Brückner Jan Heidemann Tobias M Nowacki Friederike Cordes Jorg Stypmann Philipp Lenz Faekah Gohar Andreas Lügering Dominik Bettenworth | 2017 | World Journal of Gastroenterology2017，23，16： | 4 |
| 2 | Long-term outcomes of autoimmune pancreatitis: a multicentre, international analysis显示文摘 | Phil A Hart Terumi Kamisawa William R Brugge Jae Bock Chung Emma L Culver László Czakó Luca Frulloni Vay Liang W Go Thomas M Gress Myung-Hwan Kim Shigeyuki Kawa Kyu Taek Lee Markus M Lerch Wei-Chih Liao Matthias L?hr Kazuichi Okazaki Ji Kon Ryu Nicolas Sc | 2013 | Gut2013，，12： | 2 |
| 3 | Toward a 'Critical Mass' theory of interactive media:Universal access, interdependence and diffusion显示文摘 | | 1987 | Communication Research1987，14，5： | 1 |
| 4 | The experienced 'Sense' of a virtual community: Characteristics and process显示文摘 | Blanchard A L Markus M L | 2004 | The Data Base for Advanced In- formation Systems2004，35，1： | 1 |
| 5 | Information technology and organizational change : casual structure in theory and research 显示文摘 | Markus M L Robey D | 1988 | Management Science1988，28，5： | 1 |
| 6 | Electronic mail as the medium og managerial choice显示文摘 | Markus M L | 1994 | Organization Research1994，14，： | 1 |
| 7 | Learning from Adopter's Experiences with ERP: Problems Encountered and Success Achieved 显示文摘 | MARKUS M L AXLINE S PETRIE D | 2000 | Journal of Information Technology2000，15，： | 1 |
| 8 | Diffusion of atomic oxygen relevant to water formation in amorphous interstellarices显示文摘 | Myung W L Markus M | 2014 | The Royal Society Chemistry2014，168，1： | 1 |
| 9 | Atypical language lateralisation associated with right fronto-temporal grey matter increases-a combined fMRI and VBM study in left-sided mesial temporal lobe epilepsy patients 显示文摘 | Kristen L Markus M Jozsef J | 2012 | NeuroImage2012，59，1： | 1 |
| 10 | Foresight for a responsible future with ICT显示文摘 | Markus M L Mentzer K | 2014 | Information Systems Frontiers2014，16，3： | 1 |
| 11 | The Performance Impacts of Quick Response and Strategic Alignment in Specialty Retailing显示文摘 | Palmer J W Markus M L | 2000 | In- formation Systems Research2000，11，3： | 1 |
| 12 | Development and application of a Database of Food ingredient Fraud and Economically Motivated Adulteration from 1980-2010显示文摘 | Jeffery C M John S Markus L | 2012 | Journal of Food Science2012，77，4： | 1 |
| 13 | Cytotoxic T cells specifically induce fas on target cells, thereby facilitating exocytosis-independent induction of apoptosis显示文摘 | Markus M S Paul W Mario L | 2000 | J Immunol2000，165，： | 1 |
| 14 | Prediction of clinical cardiovascular events with ca rotid inteima-media thickness: a systematic review and meta-analysis显示文摘 | LORENZ M W MARKUS H S BOTS M L | 2007 | Circulation2007，115，： | 1 |
| 15 | Cytotoxic T cells specifically induce Fas on Target cells, thereby facilitating exocytosis-independent induction of apoptosis显示文摘 | Markus M S Paul W Mario L | 2000 | J Immunol2000，165，7： | 1 |
| 16 | Electronic mail as the medium of managerial choice显示文摘 | Markus M L | 1994 | Organization Science1994，5，4： | 1 |
| 17 | Predictionofclinical cardi- ovascular events with carotid intima - media thickness : a systematic review and meta - analysis 显示文摘 | Lorenz M W Markus H S Bots M L | 2007 | Circulation2007，115，4： | 1 |
| 18 | Learning from Adopter's experiences with ERP:Problems Encountered and Success Achieved显示文摘 | Markus M L Axline S Peterie D | 2000 | Journal of Information Technology2000，15，4： | 1 |
| 19 | Power, politics, and MIS implementation显示文摘 | Markus M L | 1983 | Communications of the ACM1983，26，6： | 1 |
| 20 | The Experienced 'Sense' of a Virtual Community : Characteristics and Processes 显示文摘 | Blanchard A L Markus M L | 2004 | Database for Advances in Information Systems2004，35，1： | 1 |
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