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| 1 | Ultrasound criteria for severe in-stent restenosis following carotid artery stenting显示文摘 | Wei Zhou Deborah D. Felkai Mark Evans Sally A. McCoy Peter H. Lin Panagiotos Kougias Hosam F. El-Sayed Alan B. Lumsden | 2008 | Journal of Vascular Surgery2008，，1： | 1 |
| 2 | Knowledge Sharing显示文摘 | Peter Marks Peter Polark Scott Mccoy Dennis Gallet- ta | 2008 | Communications of the ACM2008，51，2： | 1 |
| 3 | Sharing Knowledge 显示文摘 | MARKS P POLAK P McCOY S et at | 2008 | Communications of the ACM2008，51，2： | 1 |
| 4 | Electrocardiographic manifestations of cardiac infectious-inflammatory disorders显示文摘 | Mohan Punja Dustin G. Mark Jonathan V. McCoy Ramin Javan Jesse M. Pines William Brady | 2010 | American Journal of Emergency Medicine2010，，3： | 1 |
| 5 | Gas chromatographic quantification of fatty acid methyl esters: Flame ionization detection vs. Electron impact mass spectrometry显示文摘 | Eric D. Dodds Mark R. McCoy Lorrie D. Rea John M. Kennish | 2005 | Lipids2005，，4： | 1 |
| 6 | The most important issues in knowledge management 显示文摘 | King W R Marks P V McCoy S | 2002 | Communications of the ACM2002，45，9： | 1 |
| 7 | Electrocardiographic manifestations of cardiac infectious-inflammatory disorders显示文摘 | Punja M Mark DG McCoy JV | 2010 | Am J Emerg Med2010，28，3： | 1 |
| 8 | Characterization of large-pore polymer supports for use in perfusion biochromatography显示文摘 | Mccoy Mark Gordon Neal | 1998 | J Chromatography A1998，807，2： | 1 |
| 9 | Quantification of soluble epoxide hydrolase inhibitors in experimental and clinical samples using the nanobody-based ELISA显示文摘To ensure proper dosage of a drug,analytical quantification of it in biofluid is necessary.Liquid chromatography mass spectrometry(LC-MS)is the conventional method of choice as it permits accurate identification and quantification.However,it requires expensive instrumentation and is not appropriate for bedside use.Using soluble epoxide hydrolase(sEH)inhibitors(EC5026 and TPPU)as examples,we report development of a nanobody-based enzyme-linked immunosorbent assay(ELISA)for such small molecules and its use to accurately quantify the drug chemicals in human samples.Under optimized conditions,two nanobody-based ELISAs were successfully established for EC5026 and TPPU with low limits of detection of 0.085 ng/mL and 0.31 ng/mL,respectively,and two order of magnitude linear ranges with high precision and accuracy.The assay was designed to detect parent and two biologically active metabolites in the investigation of a new drug candidate EC5026.In addition,the ELISAs displayed excellent correlation with LC-MS analysis and evaluation of inhibitory potency.The results indicate that nanobody-based ELISA methods can efficiently analyze drug like compounds.These methods could be easily implemented by the bedside,in the field in remote areas or in veterinary practice.This work illustrates that nanobody based assays offer alternative and supplementary analytical tools to mass spectrometry for monitoring small molecule medicines during clinical development and therapy.Attributes of nanobody based pharmaceutical assays are discussed. | Huiyi Yang Meng Qi Qiyi He Sung Hee Hwang Jun Yang Mark McCoy Christophe Morisseau Suqing Zhao Bruce D.Hammock | 2023 | Journal of Pharmaceutical Analysis2023，13，9： | 0 |
| 10 | 选择试剂容器减少废液处理费用以提高实验效率和质量显示文摘实验室科学家用于完成研究的工作时间是有限的。在如今现代化、实验仪器密集的实验室里,每年用于一位科学家和实验室场地的开销可能超过$250000或$120/h。由于完成研究的成本高,而且时间有限,因此需要考虑许多因素来最优化的利用科学家时间。这篇文章以一个实际调查为例讨论了如下因素,包括实验室工作流程,高效液相色谱(HPLC)分离中的溶剂质量以及如何避免重复性工作,如何使用简单自动化设备来监测实验状态从而除去多步骤和减少工作频率以节省科学家的时间。当将所有小细节纳入考虑范围中时,因节省细碎的时间而对实验成本的节约和实验效率的提升具有非常重要的潜在影响。 | Jim WESTRA Qing Ping HAN Paul McCOY Rosanne ISOM Mark HAYWARD | 2011 | 生命科学仪器2011，9，1： | 0 |
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