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| 1 | Role of glycosylation in TGF-β signaling and epithelial-to-mesenchymal transition in cancer显示文摘Glycosylation is a common posttranslational modification on membrane-associated and secreted proteins that is of pivotal importance for regulating cell functions.Aberrant glycosylation can lead to uncontrolled cell proliferation,cell-matrix interactions,migration and differentiation,and has been shown to be involved in cancer and other diseases.The epithelial-to-mesenchymal transition is a key step in the metastatic process by which cancer cells gain the ability to invade tissues and extravasate into the bloodstream.This cellular transformation process,which is associated by morphological change,loss of epithelial traits and gain of mesenchymal markers,is triggered by the secreted cytokine transforming growth factor-β(TGF-β).TGF-βbioactivity is carefully regulated,and its effects on cells are mediated by its receptors on the cell surface.In this review,we first provide a brief overview of major types of glycans,namely,N-glycans,O-glycans,glycosphingolipids and glycosaminoglycans that are involved in cancer progression.Thereafter,we summarize studies on how the glycosylation of TGF-βsignaling components regulates TGF-βsecretion,bioavailability and TGF-βreceptor function.Then,we review glycosylation changes associated with TGF-β-induced epithelial-to-mesenchymal transition in cancer.Identifying and understanding the mechanisms by which glycosylation affects TGF-βsignaling and downstream biological responses will facilitate the identification of glycans as biomarkers and enable novel therapeutic approaches. | Jing Zhang Peter ten Dijke Manfred Wuhrer Tao Zhang | 2021 | Protein & Cell2021，12，2： | 4 |
| 2 | A functional Campylobacterjejuni mar4 gene results in novel glycoforms on flagellin and altered autoagglutination behavior显示文摘 | Lieke B van Alphen Manfred Wuhrer Nancy MC Bleumink-Pluym | 2008 | Microbiology2008，154，： | 1 |
| 3 | 结直肠癌、基质和正常结肠黏膜显微解剖区域N-糖组的显著多样性显示文摘Aberrant glycosylation is considered to be a hallmark of colorectal cancer(CRC),as demonstrated by various studies.While the N-glycosylation of cell lines and serum has been widely examined,the analysis of cancer-associated N-glycans from tissues has been hampered by the heterogeneity of tumors and the complexity of N-glycan structures.To overcome these obstacles,we present a study using laser capture microdissection that makes it possible to largely deconvolute distinct N-glycomic signatures originating from different regions of heterogeneous tissues including cancerous,stromal,and healthy mucosa cells.N-glycan alditols were analyzed by means of porous graphitized carbon liquid chromatographyelectrospray ionization tandem mass spectrometry,enabling the differentiation and structural characterization of isomeric species.In total,116 N-glycans were identified that showed profound differences in expression among cancer,stroma,and normal mucosa.In comparison with healthy mucosa,the cancer cells showed an increase in a2-6 sialylation and monoantennary N-glycans,as well as a decrease in bisected N-glycans.Moreover,specific sialylated and(sialyl-)LewisA/X antigen-carrying N-glycans were exclusively expressed in cancers.In comparison with cancer,the stroma showed lower levels of oligomannosidic and monoantennary N-glycans,LewisA/X epitopes,and sulfation,as well as increased expression of(core-)fucosylation and a2-3 sialylation.Our study reveals the distinct N-glycomic profiles of different cell types in CRC and control tissues,proving the necessity of their separate analysis for the discovery of cancer-associated glycans. | Di Wang Katarina Madunic Tao Zhang Guinevere S.M.Lageveen-Kammeijer Manfred Wuhrer | 2023 | Engineering2023，，7： | 0 |
| 4 | 人体前列腺特异性抗原携带含酮基-脱氧壬酮糖酸的N-聚糖显示文摘Ketodeoxynononic acid(Kdn)is a rather uncommon class of sialic acid in mammals.However,associations have been found between elevated concentrations of free or conjugated Kdn in relation to human cancer progression.Hitherto,there has been a lack of conclusive evidence that Kdn occurs on(specific)human glycoproteins(conjugated Kdn).Here,we report for the first time that Kdn is expressed on prostate-specific antigen(PSA)N-linked glycans derived from human seminal plasma and urine.Interestingly,Kdn was found only in an a2,3-linkage configuration on an antennary galactose,indicating a highly specific biosynthesis.This unusual glycosylation feature was also identified in a urinary PSA cohort in relation to prostate cancer(PCa),although no differences were found between PCa and nonPCa patients.Further research is needed to investigate the occurrence,biosynthesis,biological role,and biomarker potential of both free and conjugated Kdn in humans. | Wei Wang Tao Zhang Jan Nouta Peter Avan Veelen Noortje de Haan Theo M.de Reijke Manfred Wuhrer Guinevere S.M.Lageveen-Kammeijer | 2023 | Engineering2023，，7： | 0 |
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