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| 1 | Regulation of smooth muscle phenotype显示文摘 | Ichiro Manabe MD Ryozo Nagai MD | 2003 | Current Atherosclerosis Reports2003，，3： | 1 |
| 2 | Pro-vs anti-stenotic capacities of type-Ⅰ vs type-Ⅱ human induced pluripotent-derived endothelial cells显示文摘AIM:To verify in vivo relevance of the categorization of human vascular endothelial cells(VECs)into type-I(proproliferative)and type-II(anti-proliferative).METHODS:Endothelial layers of murine femoral arteries were removed by wire injury(WI)operation,a common technique to induce arteriostenosis.Type-I and type-II VECs produced from human induced pluripotent stem cells(iPSCs),whose characters were previously determined by their effects on the proliferation of vascular smooth muscle cells in in vitro co-culture experiments,were mixed with Matrigel?Matrix.The mixtures were injected into subcutaneous spaces around WI-operated femoral arteries for the transplanted human iPSC-derived VECs(iPSdECs)to take a route to the luminal surface via vasa vasorum,a nutrient microvessel for larger arteries.Histologies of the femoral arteries were examined over time.The presence of human iPSdECs was checked by immunostaining studies using an antibody that specifically recognizes human VECs.Degrees of stenosis of the femoral arteries were calculated after three weeks.To determine the optimal experimental condition,xenotransplantation experiments were performed under various conditions using immunocompromised mice as well as immunocompetent mice with or without administration of immunosuppressants.RESULTS:Because immunocompromised mice showed unexpected resistance to WI-induced arteriostenosis,we performed xenotransplantation experiments using immunocompetent mice along with immunosuppressant administrations.After one week,luminal surfaces of the WI-operated arteries were completely covered by human iPSdECs,showing the efficacy of our novel transplantation technique.After three weeks,type-IiPSdECs-transplanted arteries underwent total stenosis,while type-II-iPSdECs-transplanted arteries remained intact.However,untransplanted arteries of immunosuppressant-treated mice also remained intact by unknown reasons.We found that transplanted human VECs had already been replaced by murine endothelial cells by this time,indicating that a transient existence of human type-II-iPSdECs on arterial luminal surfaces can sufficiently prevent the development of stenosis.Thus,we re-performed xenotransplantation experiments using immunocompetent mice without administrating immunosuppressants and found that arteriostenosis was accelerated or prevented by transplantation of type-I or type-II iPSdECs,respectively.Similar results were obtained from the experiments using human embryonic stem cell-derived VECs at early passages(i.e.,type-II)and late passages(i.e.,type-I).CONCLUSION:Pro-and anti-stenosis capacities of type-I and type-II human iPSdECs were verified,respectively,promising a therapeutic application of allogenic iPSdECs. | Miwako Nishio Masako Nakahara Koichi Saeki Katsuhito Fujiu Hiroshi Iwata Ichiro Manabe Akira Yuo Kumiko Saeki | 2015 | World Journal of Translational Medicine2015，4，3： | 1 |
| 3 | Direct reciprocal effects of resistin and adiponectin on vascular endothelial cells: a new insight into adipocytokine–endothelial cell interactions显示文摘 | Daiji Kawanami Koji Maemura Norihiko Takeda Tomohiro Harada Takefumi Nojiri Yasushi Imai Ichiro Manabe Kazunori Utsunomiya Ryozo Nagai | 2003 | Biochemical and Biophysical Research Communications2003，，2： | 1 |
| 4 | Cardiac fibroblasts are essential for the adaptive response of the murine heart to pressure overload显示文摘 | Takeda Norifumi Manabe Ichiro Uchino Yuichi Eguchi Kosei Matsumoto Sahohime Nishimura Satoshi Shindo Takayuki Sano Motoaki Otsu Kinya Snider Paige Conway Simon J Nagai Ryozo | 2010 | Journal of Clinical Investigation2010，，1： | 1 |
| 5 | Gene expression in fibroblasts and fibrosis: involvement in cardiac hypertrophy 显示文摘 | Ichiro Manabe Takayuki Shindo Ryozo Nagai | 2002 | Cireulation Research2002，91，12： | 1 |
| 6 | Cardiac fibroblasts are essential for the adaptive response of the murine heart to pressure overload 显示文摘 | Takeda Norifumi Manabe Ichiro Uchino Yuichi | 2010 | J Clin Invest2010，120，1： | 1 |
| 7 | C-reactive protein induces VCAM-1 gene expression through NF-κB activation in vascular endothelial cells显示文摘 | Daiji Kawanami Koji Maemura Norihiko Takeda Tomohiro Harada Takefumi Nojiri Tetsuya Saito Ichiro Manabe Yasushi Imai Ryozo Nagai | 2005 | Atherosclerosis2005，，1： | 1 |
| 8 | Cardiac fibroblasts are essential for the adaptive response of the murine heart to pressure overload显示文摘 | Takeda Norifumi Manabe Ichiro Uchino Yuichi Eguchi Kosei Matsumoto Sahohime Nishimura Satoshi Shindo Takayuki Sano Motoaki Otsu Kinya Snider Paige Conway Simon J Nagai Ryozo | 2010 | Journal of Clinical Investigation2010，，1： | 1 |
| 9 | Direct reciprocal effects of resistin and adiponectin on vascular endothelial cells: a new insight into adipocytokine–endothelial cell interactions显示文摘 | Daiji Kawanami Koji Maemura Norihiko Takeda Tomohiro Harada Takefumi Nojiri Yasushi Imai Ichiro Manabe Kazunori Utsunomiya Ryozo Nagai | 2003 | Biochemical and Biophysical Research Communications2003，，2： | 1 |
| 10 | Gene Expression in Fibroblasts and Fibrosis: Involvement in Cardiac Hypertrophy显示文摘 | Ichiro Manabe Takayuki Shindo Ryozo Nagai | 2002 | Circulation Research: Journal of the American Heart Association2002，，12： | 1 |
| 11 | Cardiovascular Inflammation显示文摘 | Masanori Aikawa Ichiro Manabe Adrian Chester Elena Aikawa | 2012 | International Journal of Inflammation2012，，： | 1 |
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