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| 1 | Expression and reconstitution of the bioluminescent Ca2+reporter aequorin in human embryonic stem cells,and exploration of the presence of functional IP3 and ryanodine receptors during the early stages of their differentiation into cardiomyocytes显示文摘In order to develop a novel method of visualizing possible Ca^(2+) signaling during the early differentiation of h ESCs into cardiomyocytes and avoid some of the inherent problems associated with using fluorescent reporters, we expressed the bioluminescent Ca^(2+) reporter, apo-aequorin, in HES2 cells and then reconstituted active holo-aequorin by incubation with f-coelenterazine. The temporal nature of the Ca^(2+) signals generated by the holo-f-aequorin-expressing HES2 cells during the earliest stages of differentiation into cardiomyocytes was then investigated. Our data show that no endogenous Ca^(2+) transients(generated by release from intracellular stores) were detected in 1–12-day-old cardiospheres but transients were generated in cardiospheres following stimulation with KCl or Ca Cl_2, indicating that holo-f-aequorin was functional in these cells. Furthermore, following the addition of exogenous ATP, an inositol trisphosphate receptor(IP_3R) agonist, small Ca^(2+) transients were generated from day 1 onward. That ATP was inducing Ca^(2+) release from functional IP_3 Rs was demonstrated by treatment with 2-APB, a known IP_3 R antagonist. In contrast, following treatment with caffeine, a ryanodine receptor(Ry R) agonist, a minimal Ca^(2+) response was observed at day 8 of differentiation only. Thus, our data indicate that unlike Ry Rs, IP_3 Rs are present and continually functional at these early stages of cardiomyocyte differentiation. | Harvey Y.S.Chan Man Chun Cheung Yi Gao Andrew L.Miller Sarah E.Webb | 2016 | Science China(Life Sciences)2016，59，8： | 5 |
| 2 | Health risk assessment of abandoned agricultural soils based on heavy metal contents in Hong Kong, the world’s most populated city显示文摘 | Yu Bon Man Xiao Lin Sun Yin Ge Zhao Brenda Natalia Lopez Shan Shan Chung Sheng Chun Wu Kwai Chung Cheung Ming H Wong | 2010 | Environment International2010，，6： | 2 |
| 3 | Targeted hepatitis C screening among ex‐injection drug users in the community显示文摘 | Vincent Wai‐Sun Wong Grace Lai‐Hung Wong Angel Mei‐Ling Chim Tsz‐Fai Cheng Shirley Wing‐Yan Cheung Carol Man‐Sze Lai Kylie Joan‐Yi Szeto Sharon Tsang Stephen Ho‐Chun Wu Kenneth Kar‐Lung Yan Alex Yui Hui Desmond Chi‐Him Yiu Brian Bing‐Ying Wu David Cheung | 2014 | J Gastroenterol Hepatol2014，，1： | 1 |
| 4 | An Investigation of Effect of Stand-Off Distance on the Material Removal Characteristics and Surface Generation in Fluid Jet Polishing显示文摘Fluid jet polishing(FJP)is a versatile polishing process that has many advantages compared to other polishing processes.Stand-off distance(SOD)is one of the key parameters in flu id jet polishi ng.However,relatively little research work has been carried out to investigate its effect of SOD on material removal characteristics and surface generation in FJP.In this paper,a systematic investigation of the effect of SOD on the tool influence function and surface topography in FJP was conducted.Experiments were designed for FJP two kinds of materials corresponding to ductile and brittle materials.They are nickel copper(NiCu)alloy and BK7 optical glass,respectively.In this study,the SOD was varied from 2 to 35 mm.Analysis and discussions were made on its effect on the shape of TIF,material removal rate,and surface topography.It is interesting to note that the TIF shape becomes a Gaussian-like shape with large SOD both on NiCu and BK7,which provides a novel way to optimize the TIF in FJP.The variation of the material removal rate and surface roughness versus SOD on NiCu and BK7 were also determined from the experimental results.Moreover,the surface topography of NiCu and BK7 were characterized from the results measured from the white light interferometer and scan electron microscope.The outcome of the study provides a better understanding of the material removal characteristics and surface generation mechanism in FJP. | Chun Jin Wang Chi Fai Cheung Lai Ting Ho Yee Man Loh | 2020 | Nanomanufacturing and Metrology2020，3，2： | 1 |
| 5 | PMDinsensitive OSNR monitoring based on polarizationnulling with offcenter narrowband filtering 显示文摘 | Cheung ManHong Chen LianKuan Chan ChunKie | 2004 | IEEE Photonics Technology Letters2004，16，11： | 1 |
| 6 | Health risk assessment of abandoned agricultural soils based on heavy metal contents in Hong Kong, the world’s most populated city显示文摘 | Yu Bon Man Xiao Lin Sun Yin Ge Zhao Brenda Natalia Lopez Shan Shan Chung Sheng Chun Wu Kwai Chung Cheung Ming H Wong | 2010 | Environment International2010，，6： | 1 |
| 7 | pPeOp inhibits HGC-27 cell proliferation,migration and invasion by upregulating miR-30b-5p and down-regulating the Rac1/Cdc42 pathway显示文摘Gastric cancer is the fifth most frequently occurring and the fourth most lethal malignant cancer worldwide.A bioactive protein(pPeOp)from Omphalia lapidescens exhibits significant inhibitory effects on gastric cancer cells.miRNA deep sequencing analysis shows that miR-30b-5p is significantly upregulated in HGC-27 cells treated with pPeOp.Verification results show that the expression level of miR-30b-5p is significantly increased in HGC-27 cells after pPeOp treatment.Additionally,miR-30b-5p is significantly downregulated in clinical gastric cancer tissues compared to that in adjacent normal tissues.Following pPeOp treatment and/or transfection with miR-30b-5p mimic,the proliferation,migration,and invasion of HGC-27 cells are significantly impaired.Immunofluorescence microscopy shows that pPeOp and/or miR-30b-5p destroy(s)microfilaments and microstructures and inhibit(s)the formation of pseudopodia.Bioinformatics analysis,dual-luciferase reporter assay,and western blot analysis confirm that miR-30b-5p downregulates Rac1/Cdc42 expression and activation by targeting RAB22A.Available data indicate that miR-30b-5p plays an anti-gastric cancer role in mediating pPeOp.pPeOp upregulates miR-30b-5p expression,which in turn inhibits RAB22A expression,resulting in a reduction in the expression and activation of Rac1 and Cdc42 and their downstream targets,thus destroying the cytoskeletal structure and inhibiting the proliferation,migration,and invasion of cancer cells. | Wenjun Xu Zhenjie Fu Yuqin Xu Man Hei Cheung Yan Chen Meiai Lin Hang Wen Hang Lv Chun Liang Jianshu Lou Yitao Chen | 2022 | Acta Biochimica et Biophysica Sinica2022，54，12： | 0 |
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