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| 1 | 套式PCR检测中国江苏省猪场猪鼻支原体和猪肺炎支原体的感染(英文)显示文摘目的猪鼻支原体和猪肺炎支原体的感染在中国养猪业较为普遍,本研究对江苏省猪鼻支原体(Mycoplasma hyorhinis,Mhr)和猪肺炎支原体(Mycoplasma hyopneumoniae,Mhp)流行病学进行初步调查。方法从江苏省不同的猪场采集7、14、21、28及35日龄仔猪的鼻拭子样本,用套式PCR方法对收集的399份鼻拭子样本进行Mhr和Mhp的检测。结果江苏省不同猪场样本中Mhr的检出率为70.9%,Mhp检出率为13.5%。5周龄以下的猪Mhr的感染率要高于Mhp。其中30个样本(7.5%)的检测结果为两种病原体共感染。7-35日龄的猪Mhr的感染率随着日龄的增长呈现明显的上升趋势,但Mhp的感染率并未随着仔猪日龄的增长而显著上升。从本研究结果分析还发现,不同品种的猪Mhr的感染率存在显著差异,与其他品种猪相比姜曲海瘦肉型品种猪(Jiangquhai porcine lean strain,JQHPL)更易于感染Mhr(p<0.001),但是其Mhp的感染率与其他猪相比不存在显著性差异(P>0.05)。结论本试验调查了江苏地区猪场中仔猪感染Mhr和Mhp的情况,且研究结果表明Mhr的感染状况与日龄及品种有一定关系。 | Joyce Wanjiru Maingi 熊祺琰 韦艳娜 马庆红 纪燕 William Kimaru 华立忠 王佳 邵国青 鲍恩东 | 2014 | 中国人兽共患病学报2014，30，8： | 6 |
| 2 | 利用巢式PCR和荧光定量PCR比较检测猪场中感染猪肺炎支原体研究(英文)显示文摘[目的]2012年3月至12月从江苏泰州某猪场采集303份鼻拭子样品,利用巢式PCR和荧光定量PCR比较检测猪肺炎支原体的感染情况。[方法]采集7、14、21、28、30和35日龄的猪的鼻拭子,4℃浸泡于PBS过夜,TIANamp?细菌DNA提取试剂盒提取DNA。然后进行Mhp183的荧光定量PCR及P36的巢式PCR检测。[结果]通过巢式PCR检测,12.5%(38/303)份样品为猪肺炎支原体阳性,用荧光定量PCR检测,50.2%(152/303)的样品为阳性。两种方法检测均为阳性的样品为22份,占7.3%,两种方法检测均为阴性的样品为127份,占41.9%。两种检测方法的感染模式相同,均在7日龄和35日龄的小猪中感染率最高,巢式PCR的感染率分别为15.6%和18.4%,荧光定量PCR的感染率分别为53.1%和56.6%。[结论]两种PCR方法均适用于猪场感染状态的检测。 | William Keeru KIMARU 白方方 武昱孜 Joyce Wanjiru MAINGI 华利忠 刘茂军 张旭 邵国青 鲍恩东 | 2014 | Agricultural Science & Technology2014，15，6： | 2 |
| 3 | Spontaneous spinal epidural hematoma in a patient with a mechanical aortic valve taking warfarin显示文摘 | Maingi M Glynn MF Scully HE et a1 | 1995 | Can J Cardiol1995，11，： | 1 |
| 4 | Quantifying hydrologic impacts following dam construction along the Tana River, Kenya显示文摘 | MAINGI J K MARSH S E | 2002 | Journal of Arid Environments2002，50，1： | 1 |
| 5 | Factors influencing wildfire occurrenceand distribution in eastern Kentucky,USA 显示文摘 | Maingi J K Henry M C | 2007 | InternationalJournal of Wildland Fire2007，16，1： | 1 |
| 6 | Gastrointestinal parasites offree-ranging colobus monkeys(Colobus angolensis palliates)in Kwale Dis-trict,Kenya coast显示文摘 | OKANGA S MUCHEMI G MAINGI N | 2006 | African Journal of Ecology2006，44，： | 1 |
| 7 | Assessment of environmental impacts of river basin development on the river in forests of eastern Kenya using multi-temporal satellite data显示文摘 | Maingi J K Marsh S E | 2001 | International Journal of Remote Sensing2001，22，： | 1 |
| 8 | A survey of anthelmintic resistance in nematode parasites of goats in Denmark显示文摘 | Maingi N Bjorn H Thamsborg S M | 1996 | Vet Parasitol1996，66，12： | 1 |
| 9 | Strong Convergence of Projected Subgradient Methods for Nonsmooth and Nonstrietly Convex Minimiza- tion 显示文摘 | MAINGIE P E | 2008 | Set-Valued and Variational Analysis2008，16，78： | 1 |
| 10 | Physics of slow L-H transitions in Dm-D Tokamak显示文摘 | Colchin R J Carreras B A Maingi R | 2002 | Nuclear Fusion2002，42，9： | 1 |
| 11 | 查看详情显示文摘 | Canik J M Maingi R Soukhanovskii V A Bell R E Kugel H W LeBlanc B P Osborne T H | | 0，，： | 1 |
| 12 | Factors influencing wildfire oc- currence and distribution in eastern Kentucky, USA 显示文摘 | Maingi JK Henry MC | 2007 | International Journal of Wildland Fire2007，16，1： | 1 |
| 13 | Effect of Plasma Shaping on Performance in the National Spherical Torus Experiment显示文摘 | GATES D A MAINGI R MENARD J | 2006 | Phys-ics of Plasmas2006，13，05： | 1 |
| 14 | Effect of selection pressure for thiabendazole resistance on fitness of Haemonchus contortus in sheep 显示文摘 | Maingi N Scott ME Prichard RK | 1990 | Parasitol1990，100，2： | 1 |
| 15 | The relationship be tween faecal egg count reduction and the lethal dose 50% in the egg hatch assay and larval development assay显示文摘 | MAINGI N BJORN H DANGOLLA A | 1998 | Vet Parasitol1998，77，23： | 1 |
| 16 | Composition, structure, and regeneration patterns in a gallery forest along the Tana River near Bura, Kenya 显示文摘 | Maingi J K Marsh S E | 2006 | Forest Ecol Manag2006，236，： | 1 |
| 17 | Recent Results From the National Spherical Torus Experiment显示文摘 | Maingi R Bell MG Bell RE | 2003 | Plasma Physics and Controlled Fusion2003，45，5： | 1 |
| 18 | Genomic organization and fine mapping of the keratin 2e gene (KRT2E): K2e V1 domain polymorphism and novel mutations in ichthyosis bullosa of Siemens显示文摘 | Smith FJ Maingi C Covello SP | 1998 | J Invest Dermatol1998，111，5： | 1 |
| 19 | Modeling of Stochastic Magnetic Perturbation by RWMEF-Coils on NSTX显示文摘 | YAN Longwen T.E. Evans S. M. Kaye R. Maingi | 2005 | Southwestern Institute of Physics Annual Report2005，，1： | 0 |
| 20 | Assessing the incidence of common bean(Phaseolus vulgaris L.)infection by fungi during harvest and storage in Cameroon显示文摘In most parts of Cameroon particularly the Menoua Division,common bean(Phaseolus vulgaris L.)is usually consumed immediately after harvest or after a few months of storage.Contamination of common bean by fungi usually occurs in the field and during storage.It is possible that the incidence of food contamination by fungi varies between field and storage.The study aimed at determining the incidence of common bean infection by fungi during harvest and during storage.Six common bean cultivars both during harvest and storage were evaluated.Analysis of variance showed no significant difference(P˃0.05)between their incidences of infection by fungi during harvest.The incidence of common bean infection during storage was shown to differ significantly between each cultivar(P≤0.05).A comparison of the mean incidence level of infection by fungi in the two groups of bean cultivars(cultivars during harvest and cultivars during storage)using the independent t-test analysis revealed a significant difference between their mean incidence of infection.The findings conclude that the incidence of common bean infection by fungi during storage is higher than during harvest in the Menoua Division.These findings will help to sensitise farmers on the improvement of bean storage conditions. | Akwa TE Maingi JM Birgen JK | 2020 | Studies in Fungi2020，5，1： | 0 |
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