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| 1 | Proposed international clinical diabetic retinopathy and diabetic macular edema disease severity scales显示文摘 | C.P Wilkinson Frederick L Ferris Ronald E Klein Paul P Lee Carl David Agardh Matthew Davis Diana Dills Anselm Kampik R Pararajasegaram Juan T Verdaguer | 2003 | Ophthalmology2003，，9： | 4 |
| 2 | Macrophage secretory products induce an inflammatory phenotype in hepatocytes显示文摘AIM:To investigate the influence of macrophages on hepatocyte phenotype and function.METHODS:Macrophages were differentiated from THP-1 monocytes via phorbol myristate acetate stimulation and the effects of monocyte or macrophageconditioned medium on HepG2 mRNA and protein expression determined.The in vivo relevance of these findings was confirmed using liver biopsies from 147 patients with hepatitis C virus(HCV)infection.RESULTS:Conditioned media from macrophages,but not monocytes,induced a transient morphological change in hepatocytes associated with upregulation of vimentin(7.8±2.5-fold,P=0.045)and transforming growth factor(TGF)-β1(2.6±0.2-fold,P<0.001)and downregulation of epithelial cadherin(1.7±0.02-fold,P=0.017)mRNA expression.Microarray analysis revealed significant upregulation of lipocalin-2(17-fold,P <0.001)and pathways associated with inflammation,and substantial downregulation of pathways related to hepatocyte function.In patients with chronic HCV,realtime polymerase chain reaction and immunohistochemistry confirmed an increase in lipocalin-2 mRNA(F0 1.0 ±0.3,F1 2.2±0.2,F2 3.0±9.3,F3/4 4.0±0.8,P= 0.003)and protein expression(F1 1.0±0.5,F2 1.3± 0.4,F3/4 3.6±0.4,P=0.014)with increasing liver injury.High performance liquid chromatography-tandem mass spectrometry analysis identified elevated levels of matrix metalloproteinase(MMP)-9 in macrophageconditioned medium,and a chemical inhibitor of MMP-9 attenuated the change in morphology and mRNA expression of TGF-β1(2.9±0.2 vs 1.04±0.1,P<0.001) in macrophage-conditioned media treated HepG2 cells.In patients with chronic HCV infection,hepatic mRNA expression of CD163(F0 1.0±0.2,F1/2 2.8±0.3,F3/4 5.3±1.0,P=0.001)and MMP-9(F0 1.0±0.4,F1/2 2.8±0.3,F3/4 4.1±0.8,P=0.011)was significantly associated with increasing stage of fibrosis.CONCLUSION:Secreted macrophage products alter the phenotype and function of hepatocytes,with increased expression of inflammatory mediators,suggesting that hepatocytes actively participate in liver injury. | Michelle Melino Victoria L Gadd Gene V Walker Richard Skoien Helen D Barrie Dinesh Jothimani Leigh Horsfall Alun Jones Matthew J Sweet Gethin P Thomas Andrew D Clouston Julie R Jonsson Elizabeth E Powell | 2012 | World Journal of Gastroenterology2012，18，15： | 3 |
| 3 | Senescent human hepatocytes express a unique secretory phenotype and promote macrophage migration显示文摘AIM:To develop a model of stress-induced senescence to study the hepatocyte senescence associated secretory phenotype(SASP).METHODS:Hydrogen peroxide treatment was used to induce senescence in the human Hep G2 hepatocyte cell line.Senescence was confirmed by cytochemical staining for a panel of markers including Ki67,p21,heterochromatin protein 1β,and senescence-associated-β-galactosidase activity.Senescent hepatocytes were characterised by gene expression arrays and quantitative polymerase chain reaction(q PCR),and conditioned media was used in proteomic analyses,a human chemokine protein array,and cell migration assays to characterise the composition and function of the hepatocyte SASP.RESULTS:Senescent hepatocytes induced classical markers of senescence(p21,heterochromatin protein1β,and senescence-associated-β-galactosidase activity);and downregulated the proliferation marker,Ki67.Hepatocyte senescence induced a 4.6-fold increase in total secreted protein(P=0.06)without major alterations in the protein profile.Senescence-induced genes were identified by microarray(Benjamini Hochbergcorrected P<0.05);and,consistent with the increase in secreted protein,gene ontology analysis revealed a significant enrichment of secreted proteins among inducible genes.The hepatocyte SASP included characteristic factors such as interleukin(IL)-8 and IL-6,as well as novel components such as SAA4,IL-32and Fibrinogen,which were validated by q PCR and/or chemokine protein array.Senescent hepatocyteconditioned medium elicited migration of inflammatory(granulocyte-macrophage colony stimulating factor,GM-CSF-derived),but not non-inflammatory(CSF-1-derived)human macrophages(P=0.022),which could contribute to a pro-inflammatory microenvironment in vivo,or facilitate the clearance of senescent cells.CONCLUSION:Our novel model of hepatocyte senescence provides insights into mechanisms by which senescent hepatocytes may promote chronic liver disease pathogenesis. | Katharine M Irvine Richard Skoien Nilesh J Bokil Michelle Melino Gethin P Thomas Dorothy Loo Brian Gabrielli Michelle M Hill Matthew J Sweet Andrew D Clouston Elizabeth E Powell | 2014 | World Journal of Gastroenterology2014，20，47： | 2 |
| 4 | Proposed international clinical diabetic retinopathy and diabetic macular edema disease severity scales显示文摘 | C.P Wilkinson Frederick L Ferris Ronald E Klein Paul P Lee Carl David Agardh Matthew Davis Diana Dills Anselm Kampik R Pararajasegaram Juan T Verdaguer | 2003 | Ophthalmology2003，，9： | 2 |
| 5 | Adaptation of HIV-1to human leukocyte antigen class I显示文摘 | Kawashima Y Pfafferott K Frater J Matthews P Payne R Addo M Gatanaga H Fujiwara M Hachiya A Koizumi H Kuse N Oka S Duda A Prendergast A Crawford H Leslie A Brumme Z Brumme C Allen T Brander C Kaslow R Tang J Hunter E Allen S Mulenga J Branch S Roach T John M Mallal S Ogwu A Shapiro R Prado J G Fidler S Weber J Pybus O G Klenerman P Ndung'u T Phillips R Heckerman D Harrigan P R Walker B D Takiguchi M Goulder P | 2009 | Nature2009，458，7238： | 1 |
| 6 | 显示文摘 | Ann E Visser Richard P Swatloski W Matthew Reichert | 2001 | Chem Commun2001，，： | 1 |
| 7 | Functional subdivisions within anterior cingulated cortex and their relationship to autonomic nervous system function显示文摘 | Matthews S C Paulus M P Simmons A N Nelesen R A Dimsdale J E | 2004 | NeuroImage2004，22，： | 1 |
| 8 | Vehicle detection and recognition in grey scale imagery显示文摘 | Matthews N D An P E Charnley D | 1996 | Control Engineering Practice Printed in Great Britain1996，4，4： | 1 |
| 9 | Enhanced pre-synaptic glutamate release in deep-dorsal horn contributes to calcium channel alpha-2-delta-1 protein-mediated spinal sensitization and behavioral hypersensitivity显示文摘 | Nguyen D Deng P Matthews E A | | 0，，： | 1 |
| 10 | Acute endophthalmitis following intravitreal triamcinolone acetonide injection显示文摘 | Darius M Moshfeghi Peter K Kaiser Ingrid U Scott Jonathan E Sears Matthew Benz Juan P Sinesterra Richard S Kaiser Sophie J Bakri Raj K Maturi Jonathan Belmont Paul M Beer Timothy G Murray Hugo Quiroz-Mercado William F Mieler | 2003 | American Journal of Ophthalmology2003，，5： | 1 |
| 11 | Demonstration and characterization of a transport that is encoded for in porcine jejunal epithelium by expression of mRNA in xenopus laevis oocytes 显示文摘 | Matthews J C Wong E A Bender P K | 1996 | Joumal of Animal Science1996，74，4： | 1 |
| 12 | The effect of graded concentrations of dietary DHA on growth, survival and tissue fatty acid profile of Senegal sole ( Solea senegalensis ) larvae during the Artemia feeding oeriod显示文摘 | Mireia V Alieia E Matthew P B | 2005 | Aquaculture2005，249，： | 1 |
| 13 | Heart Outcomes Prevention E-valuation(HOPE)Investigatom Reduction of cardiovascular risk by regression of electrocardiographic markers of left ventricular hypertrophy by the angiotensin-converting enzyme inhibitor ramJipril显示文摘 | Matthew J Sleight P Lonn E | | 0，，08： | 1 |
| 14 | A pancreatic islet-specif- ic microRNA regulates insulin secretion显示文摘 | Matthew N P Lene E Jan K | 2004 | Nature2004，432，： | 1 |
| 15 | Mass fractionation processes of transition metal isotopes显示文摘 | Zhu X K Guo Y Williams R J P O'Nions R K Matthews A Belshaw N S Canters G W de Waal E C Weser U Burgess B K Salvato B | 2002 | Earth Planet Sci Lett2002，200，12： | 1 |
| 16 | Operating in the green: modeling eco-friendly telecom network management 显示文摘 | Matthews E P Nici J Polonsky B | 2010 | Belt LABS Technical Journal2010，15，2： | 1 |
| 17 | Demonstration and characterization of dipeptide transport system activity in sheep omasal epithelium by expression of mRNA in Xenopus laevis oocytes显示文摘 | Matthews J C Wong E A Bender P K | 1996 | J Anim Sci1996，70，： | 1 |
| 18 | CXCR4 regulates growth of both primary and metastatic breast cancer显示文摘 | MATTHEW C P SMITH KATHRYN E LUKER JOEL R GARBOW | 2004 | Cancer Res2004，64，： | 1 |
| 19 | Gene duplication in the carotenoid biosynthetic pathway preceded evolution of the grasses (Poaceae) 显示文摘 | Gallagher C E Matthews P D Li F | 2004 | Plant Physiol2004，135，： | 1 |
| 20 | Stabilization mechanisms of hindered amines 显示文摘 | PETER P KLEMCHUK G MATTHEW E | 1988 | Polymer Degradation and Stability1988，22，3： | 1 |
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