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| 1 | Anthropogenic emission inventories in China:a review显示文摘The development of reliable anthropogenic emission inventories is essential for both understanding the sources of air pollution and designing effective air-pollution-control measures in China. However, it is challenging to quantify emissions in China accurately, given the variety of contributing sources, the complexity of the technology mix and the lack of reliable measurements. Over the last two decades,tremendous efforts have been made to improve the accuracy of emission inventories, and significant improvements have been realized. More reliable statistics and survey-based data have been used to reduce the uncertainties in activity rates and technology distributions. Local emission factors and source profiles covering various sources have been measured and reported. Based on these local databases, improved emission inventory models have been developed for power plants, large industrial plants and the residential,transportation and agricultural sectors. In this paper, we review the progress that has been made in developing inventories of anthropogenic emissions in China. We first highlight the major updates that have been made to emission inventory models and the underlying data by source category. We then summarize the sector-based estimates of emissions of different species contained in current inventories. The progress that has been made in the development of model-ready emissions is also presented. Finally, we suggest future directions for further improving the accuracy of emission inventories in China. | Meng Li Huan Liu Guannan Geng Chaopeng Hong Fei Liu Yu Song Dan Tong Bo Zheng Hongyang Cui Hanyang Man Qiang Zhang Kebin He | 2017 | National Science Review2017，4，6： | 100 |
| 2 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 3 | Phase 1 clinical trial demonstrated that MUC1 positive metastatic seminal vesicle cancer can be effectively eradicated by modified Anti-MUC1 chimeric antigen receptor transduced T cells显示文摘Recent progress in chimeric antigen receptor-modified T-cell(CAR-T cell) technology in cancer therapy is extremely promising, especially in the treatment of patients with B-cell acute lymphoblastic leukemia. In contrast, due to the hostile immunosuppressive microenvironment of a solid tumor, CAR T-cell accessibility and survival continue to pose a considerable challenge, which leads to their limited therapeutic efficacy. In this study, we constructed two anti-MUC1 CAR-T cell lines. One set of CAR-T cells contained SM3 single chain variable fragment(sc Fv) sequence specifically targeting the MUC1 antigen and co-expressing interleukin(IL) 12(named SM3-CAR). The other CAR-T cell line carried the SM3 sc Fv sequence modified to improve its binding to MUC1 antigen(named p SM3-CAR) but did not co-express IL-12. When those two types of CAR-T cells were injected intratumorally into two independent metastatic lesions of the same MUC1+ seminal vesicle cancer patient as part of an interventional treatment strategy, the initial results indicated no side-effects of the MUC1 targeting CAR-T cell approach, and patient serum cytokines responses were positive. Further evaluation showed that p SM3-CAR effectively caused tumor necrosis, providing new options for improved CAR-T therapy in solid tumors. | Fengtao You Licui Jiang Bozhen Zhang Qiang Lu Qiao Zhou Xiaoyang Liao Hong Wu Kaiqi Du Youcai Zhu Huimin Meng Zhishu Gong Yunhui Zong Lei Huang Man Lu Jirong Tang Yafen Li Xiaochen Zhai Xiangling Wang Sisi Ye Dan Chen Lei Yuan Lin Qi Lin Yang | 2016 | Science China(Life Sciences)2016，59，4： | 16 |
| 4 | Wheat straw-derived magnetic carbon foams: In-situ preparation and tunable high-performance microwave absorption显示文摘Recently,biomass-derived three-dimensional (3D) porous carbon materials have been gaining more interest as promising microwave absorbers due to their low cost,vast availability,and sustainability.Here,a novel 3D interconnected porous magnetic carbon foams are in-situ synthesized via a combination of sol-gel and carbonization process with wheat straw as the carbon source and FeCl3·6H2O as the magnetic regulating agent.During the process of foams formation,the lignocelluloses from the steam-exploded wheat straw are converted into interconnected carbon sheet networks with hierarchical porous structures,and the precursor FeCl3·6H2O is converted into magnetic nanoparticles uniformly embedded in the porous carbon foams.The generated magnetic nanoparticles are benefit to enhance the interface polarization and magnetic loss ability to improve the efficient complementarities between the dielectric and magnetic loss,thus increasing the impedance matching.The obtained sample treated at 600 ℃ displays the best microwave absorption (MA) performance.It presents a minimal reflection loss (RL) of-43.6 dB at 7.1 GHz and the effective bandwidth (RL <-10 dB) is 3.3 GHz with the thickness of 4.7 mm.The 3D porous structure,multi-interfaces and the synergy of dielectric loss and magnetic loss make great contribution to the outstanding MA performance. | Guangjun Gou Fanbin Meng Huagao Wang Man Jiang Wei Wei Zuowan Zhou | 2019 | Nano Research2019，12，6： | 9 |
| 5 | Long-term postoperative outcomes of bilateral lateral rectus recession vs unilateral recession-resection for intermittent exotropia显示文摘AIM: To discuss the long-term postoperative results of bilateral lateral rectus recession(BLR) and unilateral lateral rectus recession-medial rectus resection(RR) in therapy of intermittent exotropia.METHODS: We retrospectively analyzed 213 cases of intermittent exotropia who underwent surgery between2008 and 2010. The patients were grouped into BLR group and RR group. Motor outcomes were divided into three groups on the basis of the angle of deviation after surgery: overcorrection(esotropia/phoria >5△), orthophoria(esotropia/phoria ≤5△to exotropia/phoria ≤10△), and undercorrection/recurrence(exotropia/phoria >10△).Titmus test was used to evaluate stereoacuity, the stereoacuity <800s of arc meaned the patients had stereopsis. Surgical outcome including motor criteria and sensory status were compared at postoperative 6, 12,24 mo and at 36 mo examination between groups. RESULTS: At 12, 24 mo after surgery, the motor outcomes had no difference(P >0.05) between groups.However, the motor outcomes at 6, 36 mo were signally different in each group, indicating the success rate in RR group at 6mo was higher than that in BLR group(83.02%vs 82.24%, P <0.05) but the result was contrary at the 3y examination(60.75% vs 43.40%, P <0.05). No statistical significance were found in the sensory outcomes between the groups at mean of 3.7y follow-up.CONCLUSION: The motor outcomes in RR group were better than in BLR group at 6mo after surgery, while the3 y outcomes were better in BLR group. This may be due to the recurrence rate of the BLR was lower than the RR group's. | Xian Yang Teng-Teng Man Qiao-Xia Tian Gui-Qiu Zhao Qing-Lan Kong Yan Meng Yan Gao Mei-Zhen Ning | 2014 | International Journal of Ophthalmology(English edition)2014，7，6： | 8 |
| 6 | Enriched environment enhances histone acetylation of NMDA receptor in the hippocampus and improves cognitive dysfunction in aged mice显示文摘The mechanisms of age-associated memory impairment may be associated with glutamate receptor function and chromatin modification.To observe the effect of an enriched environment on the cognitive function of mice with age-associated memory impairment,3-monthold C57BL/6 male mice('young'mice)were raised in a standard environment,while 24-month-old C57BL/6 male mice with memory impairment('age-associated memory impairment'mice)were raised in either a standard environment or an enriched environment.The enriched environment included a variety of stimuli involving movement and sensation.A water maze test was then used to measure cognitive function in the mice.Furthermore,quantitative real-time polymerase chain reaction and western blot assays were used to detect right hippocampal GluN2B mRNA as well as protein expression of GluN2B and CREB binding protein in all mice.In addition,chromatin immunoprecipitation was used to measure the extent of histone acetylation of the hippocampal GluN2B gene promoters.Compared with the young mice,the water maze performance of age-associated memory impairment mice in the standard environment was significantly decreased.In addition,there were significantly lower levels of total histone acetylation and expression of CREB binding protein in the hippocampus of age-associated memory impairment mice in the standard environment compared with the young mice.There were also significantly lower levels of histone acetylation,protein expression,and mRNA expression of GluN2B in the hippocampus of these mice.In contrast,in the age-associated memory impairment mice with the enriched environment intervention,the water maze performance and molecular biological indexes were significantly improved.These data confirm that an enriched environment can improve cognitive dysfunction in age-associated memory impairment mice,and suggest that the mechanisms may be related to the increased expression of CREB binding protein and the increased degree of total histone acetylation in the hippocampus of age-associated memory impairment mice,which may cause the increase of histone acetylation of GluN2B gene promoter and the enhancement of GluN2B mRNA transcription and protein expression in hippocampus.The animal experiment was approved by the Animal Ethics Committee of Yangzhou University,China(approval No.20170312001)in March 2017. | Xin Wang Zhao-Xiang Meng Ying-Zhu Chen Yu-Ping Li Hong-Yu Zhou Man Yang Ting-Ting Zhao Yu-Lai Gong Yi Wu Tao Liu | 2020 | Neural Regeneration Research2020，15，12： | 8 |
| 7 | Surface free energy of copper-zinc alloy for energy-saving of boiler显示文摘Cu-Zn, Cu-Zn-Sn, Cu-Zn-Ni alloys were melted by vacuum smelter. The effect factors to the surface free energy of the alloys such as chemical composition, crystal structure and surface crystal lattice distortion etc. were investigated by OCA30 automatic contact angle test instrument, metallography microscope and XRD instrument etc. Results suggests: adding alloy element to Cu may increase its surface free energy, and the more kinds of alloy elements are added, the more surface free energy increases; the alloy element Sn an increase the surface free energy of Cu-Zn alloy; Cu-Zn alloy with fir-tree crystal structure, great phase discrepancy and obvious composition aliquation has greater surface free energy; Cu-Zn alloy with compounds and serious surface crystal lattice distortion has greater surface free energy. | WANG Man LIANG Jinsheng TANG Qingguo MING Xing MENG Junping DING Yan | 2006 | Rare Metals2006，25，z1： | 6 |
| 8 | Analytical Solutions for Predicting Tensile and Shear Moduli of Triaxial Weave Fabric Composites显示文摘Novel micromechanical curved beam models were presented for predicting the tensile and shear moduli of triaxial weave fabric(TWF) composites by considering the interactions between the triaxial yarns of 0?and±60?. The triaxial yarns in micromechanical representative unit cell(RUC) were idealized as curved beams with a path depicted using the sinusoidal shape functions, and the tensile and shear moduli of TWF composites were derived by means of the strain energy approach founded on micromechanics. In order to validate the new models, the predictions were compared with the experimental data from literature. It was shown that the predictions from the new model agree well with the experimental results. Using these models, the tensile and shear properties of TWF composites could be predicted based only on the properties of basic woven fabric. | Jiangbo Bai Junjiang Xiong Meng Liu Ziyu Man | 2016 | Acta Mechanica Solida Sinica2016，29，1： | 6 |
| 9 | Superior de/hydrogenation performances of MgH2 catalyzed by 3D flower-like TiO2@C nanostructures显示文摘Magnesium hydride has been seen as a potential material for solid state hydrogen storage,but the kinetics and thermodynamics obstacles have hindered its development and application.Three-dimensional flower-like TiO2@C and TiO2 were synthesized as the catalyst for MgH2 system and great catalytic activities are acquired in the hydrogen sorption properties.Experiments also show that the flower-like TiO2@C is superior to flower-like TiO2 in improving the hydrogen storage properties of MgH2.The hydrogen desorption onset and peak temperatures of flower-like TiO2 doped MgH2 is reduced to 199.2℃and 245.4℃,while the primitive MgH2 starts to release hydrogen at 294.6℃and the rapid dehydrogenation temperature is even as high as 362.6℃.The onset and peak temperatures of flower-like TiO2@C doped MgH2 are further reduced to 180.3℃and 233.0℃.The flower-like TiO2@C doped MgH2 composite can release6.0 wt%hydrogen at 250℃within 7 min,and 4.86 wt%hydrogen at 225℃within 60 min,while flowerlike TiO2 doped MgH2 can release 6.0 wt%hydrogen at 250℃within 8 min,and 3.89 wt%hydrogen at225℃within 60 min.Hydrogen absorption kinetics is also improved dramatically.Moreover,compared with primitive MgH2 and the flower-like TiO2 doped MgH2,the activation energy of flower-like TiO2@C doped MgH2 is significantly decreased to 67.10 kJ/mol.All the improvement of hydrogen sorption properties can be ascribed to the flower-like structure and the two-phase coexistence of TiO2 and amorphous carbon.Such phase composition and unique structure are proved to be the critical factor to improve the hydrogen sorption properties of MgH2,which can be considered as the new prospect for improving the kinetics of light-metal hydrogen storage materials. | Meng Zhang Xuezhang Xiao Bosang Luo Meijia Liu Man Chen Lixin Chen | 2020 | Journal of Energy Chemistry2020，29，7： | 5 |
| 10 | Corrigendum to Anthropogenic emission inventories in China:a review显示文摘In the Figure 4 of ‘Anthropogenic emission inventories in China:a review'(National Science Review,Volume 4,Issue 6, 2017,Pages 834-866,doi:10.1093/nsr/nwx150),the data for the MEIC vl.2 was incorrectly plotted.The corrected version of Figure 4 is presented below. | Meng Li Huan Liu Guannan Geng Chaopeng Hong Fei Liu Yu Song Ban Tong Bo Zheng Hongyang Cui Hanyang Man Qiang Zhang Kebin He | 2018 | National Science Review2018，5，4： | 5 |
| 11 | Comparable effects of Jiedu Granule, a compound Chinese herbal medicine, and sorafenib for advanced hepatocellular carcinoma: A prospective multicenter cohort study显示文摘Objective:Sorafenib has been extensively used for the treatment of advanced hepatocellular carcinoma(HCC),and Chinese herbal medicine has also been used to manage advanced HCC.The present work evaluates the effectiveness and safety of Jiedu(JD)Granule,a compound of traditional Chinese herbal medicine,side-by-side with sorafenib for the treatment of advance HCC.Methods:Patients with advanced HCC receiving treatment with JD Granule or sorafenib were enrolled from December 2014 to March 2018.The primary endpoint was overall survival(OS).The secondary endpoints were progression-free survival(PFS)and safety.Propensity score matching(PSM)analysis was used to control for possible selection bias from the study group allocation process.Results:Of the 325 patients included,161 received JD Granule and 164 received sorafenib.No significant differences were found in OS or PFS among patients receiving JD Granule compared to sorafenib(P>0.05).Median OS of the two study groups was 6.83 months(95%confidence interval[CI]:5.83–9.47)in the group receiving JD Granule and 8 months(95%CI:6.67–9.80)in the group receiving sorafenib,with half-,1-and 2-year survival rates of 53.6%,31.2%and 13.2%vs 60.1%,35.5%and 14.2%,respectively.Even after PSM,the median survival time did not differ between the JD Granule group(9.03 months;95%CI:6.37–14.2)and the sorafenib group(7.93 months;95%CI:6.5–9.97),with comparable half-,1-and 2-year survival rates.The most common adverse events(AEs)were diarrhea(13.7%)and fatigue(5.6%)in the JD Granule group,and hand-foot skin reaction(46.3%)and diarrhea(36.6%)in the sorafenib group.The JD Granule was more cost-effective than sorafenib treatment for advanced HCC.Conclusion:Compared to sorafenib,JD Granule was more cost-effective and caused fewer AEs for the treatment of Chinese patients with advanced HCC. | He-tong Zhao Yong-bin Meng Xiao-feng Zhai Bin-bin Cheng Sha-sha Yu Man Yao Hui-xia Yin Xu-ying Wan Yun-ke Yang Hui Liu Feng Shen Chang-quan Ling | 2020 | Journal of Integrative Medicine2020，18，4： | 5 |
| 12 | Association between Fine Particulate Air Pollution and Outpatient Visits for Eczema in Beijing, China: A Time-series Analysis显示文摘Eczema is a very common inflammatory skin disease characterized by patches of erythematous,itchy, cracked, and scaly skin.According to the 2010 Global Burden of Disease Study, the global prevalence of eczema is 3.33% and the leading cause of skin condition-based disability-adjusted life years is eczema[1].The large health burden brought about by eczema suggests high direct medical costs and health care utilization[2].Thus, eczema prevention is of great importance in efforts to eliminate the public health burden of the disease. | WANG Xiao Wen TIAN Yao Hua CAO Ya Ying SONG Jing LI Man WU Yao WANG Meng Ying HUANG Zhe HU Yong Hua | 2019 | Biomedical and Environmental Sciences2019，32，8： | 4 |
| 13 | Expression, purification, and functional characterization of recombinant PTD-SARA显示文摘为蛋白质是的受体激活(SARA ) 的 Smad 锚为在支持发信号的小径的 fibrotic 起一个重要作用的 Smad2/3 的一个有约束力的搭挡由转变生长 factor-1 (TGF-1 ) 开始了。C 终端 SARA 的 665750 aa 包括 Smad 有约束力的领域(SBD ) 。由通过 SBD 的直接相互作用, SARA 禁止 Smad2/3 phosphorylation 并且堵住在 Smad2/3 和 Smad4 之间的相互作用,从而制止纤维变性的过程。在这研究,我们基于 SBD 顺序构造了 SARA 肽 aptamer。recombinant SARA aptamer,与一个蛋白质 transduction 领域(PTD-SARA ) 熔化了,被克隆,从 E 净化了。coli,并且第一次描绘了。编码顺序的全身的 PTD-SARA,与 E 创造了。coli 赞成了 codons,被克隆进 pQE-30 向量,并且 recombinant plasmid 被转变成 M15 紧张。在 Isopropyl -D-1-Thiogalactopyranoside (IPTG ) 以后正式就职和 Ni2+ 亲密关系纯化, recombinant PTD-SARA 被定序的 immunoblotting 和蛋白质 N 终端进一步识别。Epifluorescence 显微镜学表明 recombinant PTD-SARA 比 SARA 更高效地被变成细胞质和原子核。而且, recombinant PTD-SARA 被发现 E-cadherin 和下面调整的水平到起来调整 -SMA 和 phospho-Smad3 的层次更高效地比 SARA (P < 0.05 ) 。我们的工作探索了一个方法获得 recombinant PTD-SARA 蛋白质。recombinant PTD-SARA 熔化蛋白质能进入 HK2 房间(使不朽的近似小管上皮的房间线) 更高效地比 SARA 蛋白质和颠倒被 TGF-1 比 SARA 蛋白质更有效地导致的肾的 epithelial-to-mesenchymal transdifferentiation 过程。Recombinant PDT-SARA 是可能的是肾的纤维变性的临床的预防和处理的一个潜在的候选人。 | Chen Huang Rui Du Peng Zhang Hua Meng Huiwei Jia Yang Song Man Li Yingqi Zhang Shiren Sun | 2011 | Acta Biochimica et Biophysica Sinica2011，43，2： | 4 |
| 14 | Could saline irrigation clear all residual common bile duct stones after lithotripsy?A self-controlled prospective cohort study显示文摘BACKGROUND A previous study showed that irrigation with 100 mL saline reduced residual common bile duct(CBD)stones,which potentially cause recurrent stones after endoscopic retrograde cholangiopancreatography.AIM To determine whether saline irrigation can improve CBD clearance after lithotripsy.METHODS This prospective self-controlled study enrolled patients receiving mechanical lithotripsy for large(>1.2 cm)CBD stones.After occlusion cholangiography confirmed CBD stone clearance,peroral cholangioscopy(POC)was performed to determine clearance scores based on the number of residual stones.The amounts of residual stones spotted via POC were graded on a 5-point scale(score 1,worst;score 5,best).Scores were documented after only stone removal(control)and after irrigation with 50 mL and 100 mL saline,respectively.The stone composition was analyzed using infrared spectroscopy.RESULTS Between October 2018 and January 2020,47 patients had CBD clearance scores of 2.4±1.1 without saline irrigation,3.5±0.7 with 50 mL irrigation,and 4.6±0.6 with 100 mL irrigation(P<0.001).Multivariate analysis showed that CBD diameter>15 mm[odds ratio(OR)=0.08,95%confidence interval(CI):0.01-0.49;P=0.007]and periampullary diverticula(PAD)(OR=6.51,95%CI:1.08-39.21;P=0.041)were independent risk factors for residual stones.Bilirubin pigment stones constituted the main residual stones found in patients with PAD(P=0.004).CONCLUSION Irrigation with 100 mL of saline may not clear all residual CBD stones after lithotripsy,especially in patients with PAD and/or a dilated(>15 mm)CBD.Pigment residual stones are soft and commonly found in patients with PAD.Additional saline irrigation may be required to remove retained stones. | Yan-Yan Lin Yu-Dong Wang Ping Yue Xian-Zhuo Zhang Joseph W Leung Pan-Pan Jiao Man Yang Hai-Ping Wang Bing Bai Ying Liu Jin-Duo Zhang Hong-Bo Chen Wen-Bo Meng Xun Li | 2021 | World Journal of Gastroenterology2021，27，4： | 4 |
| 15 | Volcanoes and Climate:Sizing up the Impact of the Recent Hunga Tonga-Hunga Ha'apai Volcanic Eruption from a Historical Perspective显示文摘An undersea volcano at Hunga Tonga-Hunga Ha'apai(HTHH)near the South Pacific island nation of Tonga,erupted violently on 15 January 2022.Potential climate impact of the HTHH volcanic eruption is of great concern to the public;here,we intend to size up the impact of the HTHH eruption from a historical perspective.The influence of historical volcanic eruptions on the global climate are firstly reviewed,which are thought to have contributed to decreased surface temperature,increased stratospheric temperature,suppressed global water cycle,weakened monsoon circulation and El Niño-like sea surface temperature.Our understanding of the impacts of past volcanic eruptions on global-scale climate provides potential implication to evaluate the impact of the HTHH eruption.Based on historical simulations,we estimate that the current HTHH eruption with an intensity of 0.4 Tg SO_(2)injection will decrease the global mean surface temperature by only 0.004℃in the first year after eruption,which is within the amplitude of internal variability at the interannual time scale and thus not strong enough to have significant impacts on the global climate. | Meng ZUO Tianjun ZHOU Wenmin MAN Xiaolong CHEN Jian LIU Fei LIU Chaochao GAO | 2022 | Advances in Atmospheric Sciences2022，39，12： | 4 |
| 16 | 3D-printable supramolecular hydrogels with shear-thinning property: fabricating strength tunable bioink via dual crosslinking显示文摘3-dimensional(3D)bioprinting technology provides promising strategy in the fabrication of artificial tissues and organs.As the fundamental element in bioprinting process,preparation of bioink with ideal mechanical properties without sacrifice of biocompatibility is a great challenge.In this study,a supramolecular hydrogel-based bioink is prepared by polyethylene glycol(PEG)grafted chitosan,α-cyclodextrin(α-CD)and gelatin.It has a primary crosslinking structure through the aggregation of the pseudo-polyrotaxane-like side chains,which are formed from the host-guest interactions betweenα-CD and PEG side chain.Apparent viscosity measurement shows the shear-shinning property of this bioink,which might be due to the reversibility of the physical crosslinking.Moreover,withβ-glycerophosphate at different concentrations as the secondary crosslinking agent,the printed constructs demonstrate different Young's modulus(p<0.001).They could also maintain the Young's modulus in cell culture condition for at least 21 days(p<0.05).By co-culturing each component with fibroblasts,CCK-8 assay demonstrate cellular viability is higher than 80%.After bioprinting and culturing,immunofluorescence staining with quantification indicate the expression of Ki-67,Paxillin,and N-cadherin is higher in day 14 than those in day 3(p<0.05).Oil red O and Nissl body specific staining reflect strength tunable bioink may have impact on the cell fate of mesenchymal stem cells(p<0.05).This work might provide new idea for advanced bioink in the application of re-establishing complicated tissues and organs. | Tian Hu Xiaoliang Cui Meng Zhu Man Wu Ye Tian Bin Yao Wei Song Zhongwei Niu Sha Huang Xiaobing Fu | 2020 | Bioactive Materials2020，5，4： | 3 |
| 17 | Plant multiscale networks:charting plant connectivity by multi-level analysis and imaging techniques显示文摘In multicellular and even single-celled organisms,individual components are interconnected at multiscale levels to produce enormously complex biological networks that help these systems maintain homeostasis for development and environmental adaptation.Systems biology studies initially adopted network analysis to explore how relationships between individual components give rise to complex biological processes.Network analysis has been applied to dissect the complex connectivity of mammalian brains across different scales in time and space in The Human Brain Project.In plant science,network analysis has similarly been applied to study the connectivity of plant components at the molecular,subcellular,cellular,organic,and organism levels.Analysis of these multiscale networks contributes to our understanding of how genotype determines phenotype.In this review,we summarized the theoretical framework of plant multiscale networks and introduced studies investigating plant networks by various experimental and computational modalities.We next discussed the currently available analytic methodologies and multi-level imaging techniques used to map multiscale networks in plants.Finally,we highlighted some of the technical challenges and key questions remaining to be addressed in this emerging field. | Xi Zhang Yi Man Xiaohong Zhuang Jinbo Shen Yi Zhang Yaning Cui Meng Yu Jingjing Xing Guangchao Wang Na Lian Zijian Hu Lingyu Ma Weiwei Shen Shunyao Yang Huimin Xu Jiahui Bian Yanping Jing Xiaojuan Li Ruili Li Tonglin Mao Yuling Jiao Sodmergen Haiyun Ren Jinxing Lin | 2021 | Science China(Life Sciences)2021，64，9： | 3 |
| 18 | Moderate exercise prevents neurodegeneration in D-galactose-induced aging mice显示文摘D-galactose has been widely used in aging research because of its efficacy in inducing senescence and accelerating aging in animal models. The present study investigated the benefits of exercise for preventing neurodegeneration, such as synaptic plasticity, spatial learning and memory abilities, in mouse models of aging. D-galactose-induced aging mice were administered daily subcutaneous injections of D-galactose at the base of the neck for 10 consecutive weeks. Then, the mice were subjected to exercise training by running on a treadmill for 6 days a week. Shortened escape latency in a Morris water maze test indicated that exercise improved learning and memory in aging mice. The ameliorative changes were likely induced by an upregulation of Bcl-2 and brain-derived neurotrophic factor, the repression of apoptosis factors such as Fas and Bax, and an increase in the activity of glucose transporters-1 and 4. The data suggest moderate exercise may retard or inhibit neurodegeneration in D-galactose-induced aging mice. | Li Li Meng Xu Bo Shen Man Li Qian Gao Shou-gang Wei | 2016 | Neural Regeneration Research2016，11，5： | 3 |
| 19 | High-purity helical carbon nanotubes by trace-water- assisted chemical vapor deposition: Large-scale synthesis and growth mechanism显示文摘 | Fanbin Meng Ying Wang Qiang Wang Xiaoling Xu Man Jiang Xuesong Zhou Ping He Zuowan Zhou | 2018 | Nano Research2018，11，6： | 3 |
| 20 | Recent advances in mitochondria-and lysosomes-targeted small-molecule two-photon fluorescent probes显示文摘Mitochondria and lysosomes are essential cellular organelles in most eukaryotic cells by playing the physiological roles to support the normal functions of cells, as well as the life of the whole body. To date,small-molecule fluorescent probes have been considered as one of the vital tools for monitoring and visualizing multiple biological analytes. This review summarized the recent advances in small-molecule two-photon fluorescent probes for metal ions, reactive oxygen species(ROS) and reactive sulfur species(RSS), and changes inside micro-environment(e.g., p H, viscosity and polarity) in mitochondria and lysosomes, or served as mitotracker and lysotracker with the assistance of two-photon microscopy. | Peng Ning Wenjuan Wang Man Chen Yan Feng Xiangming Meng | 2017 | Chinese Chemical Letters2017，28，10： | 2 |
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