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| 1 | Diarrhoea-predominant irritable bowel syndrome distinguishable by 16S rRNA gene phylotype quantification显示文摘AIM:To study whether selected bacterial 16S ribosomal RNA(rRNA)gene phylotypes are capable of disting- uishing irritable bowel syndrome(IBS). METHODS:The faecal microbiota of twenty volunteers with IBS,subdivided into eight diarrhoea-predominant (IBS-D),eight constipation-predominant(IBS-C)and four mixed symptom-subtype(IBS-M)IBS patients,and fifteen control subjects,were analysed at three time-points with a set of fourteen quantitative real-timepolymerase chain reaction assays.All assays targeted 16S rRNA gene phylotypes putatively associated with IBS,based on 16S rRNA gene library sequence analysis. The target phylotypes were affiliated with Actinobac-teria,Bacteroidetes and Firmicutes.Eight of the target phylotypes had less than 95%similarity to cultured bacterial species according to their 16S rRNA gene sequence.The data analyses were made with repeated-measures ANCOVA-type modelling of the data and principle component analysis(PCA)with linear mixed-effects models applied to the principal component scores. RESULTS:Bacterial phylotypes Clostridium cocleatum 88%,Clostridium thermosuccinogenes 85%,Coprobacillus catenaformis 91%,Ruminococcus bromii-like, Ruminococcus torques 91%,and R.torques 93%were detected from all samples analysed.A multivariate analysis of the relative quantities of all 14 bacterial 16S rRNA gene phylotypes suggested that the intestinal microbiota of the IBS-D patients differed from other sample groups.The PCA on the first principal component(PC1),explaining 30.36%of the observed variation in the IBS-D patient group,was significantly altered from all other sample groups(IBS-D vs control, P=0.01;IBS-D vs IBS-M,P=0.00;IBS-D vs IBS-C, P=0.05).Significant differences were also observed in the levels of distinct phylotypes using relative values in proportion to the total amount of bacteria.A phy- lotype with 85%similarity to C.thermosuccinogenes was quantified in significantly different quantities among the IBS-D and control subjects(-4.08±0.90 vs -3.33±1.16,P=0.04)and IBS-D and IBS-M subjects (-4.08±0.90 vs-3.08±1.38,P=0.05).Furthermore,a phylotype with 94%similarity to R.torques was more prevalent in IBS-D patients'intestinal micro- biota than in that of control subjects(-2.43±1.49 vs -4.02±1.63,P=0.01).A phylotype with 93%simi- larity to R.torques was associated with control sam- ples when compared with IBS-M(-2.41±0.53 vs -2.92±0.56,P=0.00).Additionally,a R.bromii-like phylotype was associated with IBS-C patients in com- parison to control subjects(-1.61±1.83 vs-3.69± 2.42,P=0.01).All of the above mentioned phylotype specific alterations were independent of the effect of time. CONCLUSION:Significant phylotype level alterationsin the intestinal microbiotas of IBS patients were observed,further emphasizing the possible contribution of the gastrointestinal microbiota in IBS. | Anna Lyra Teemu Rinttil Janne Nikkil Lotta Krogius-Kurikka Kajsa Kajander Erja Malinen Jaana Mtt Laura Mkel Airi Palva | 2009 | World Journal of Gastroenterology2009，15，47： | 26 |
| 2 | Thalidomide induces mucosal healing in Crohn's disease: Case report显示文摘Crohn's disease is a chronic inflammatory disorder of the gastrointestinal tract that is defi ned by relapsing and remitting episodes. Tumor necrosis factor alpha (TNF-α) appears to play a central role in the pathophysiology of the disease. Standard therapies for inflammatory bowel disease fail to induce remission in about 30% of patients. Biological therapies have been associated with an increased incidence of infections, especially infection by Mycobacterium tuberculosis (Mtb). Thalidomide is an oral immunomodulatory agent with anti-TNF-α properties. Recent studies have suggested that thalidomide is effective in refractory luminal and fistulizing Crohn's disease. Thalidomide costimulates T lymphocytes, with greater effect on CD8+ than on CD4+ T cells, which contributes to the protective immune response to Mtb infection. We present a case of Crohn's disease with gastric, ileal, colon and rectum involvement as well as steroid dependency, which progressed with loss of response to infliximabafter three years of therapy. The thorax computed tomography scan demonstrated a pulmonary nodule suspected to be Mtb infection. The patient was started on thalidomide therapy and exhibited an excellent response. | Márcio Rios Leite Sandra Sousa Santos André Castro Lyra Jaciane Mota Genoile Oliveira Santana | 2011 | World Journal of Gastroenterology2011，17，45： | 9 |
| 3 | Structural analysis and optimization in the framework ofreduced-basis method 显示文摘 | Afonso S M B Lyra P R M Albuquerque T M M | 2010 | Structural andMultidisciplinary Optimization2010，40，16： | 1 |
| 4 | An alternative isolated wind electric pumping system using induction machines显示文摘 | MIRANDA M LYRA ROC SILVA SR | 1999 | IEEE Transactions on Energy Conversion1999，14，： | 1 |
| 5 | Hepatitis C–related hepatocellular carcinoma in the United States: influence of ethnic status显示文摘 | Adrian M Di Bisceglie Andre C Lyra Myron Schwartz Rajender K Reddy Paul Martin Gregory Gores Anna S.F Lok Khozema B Hussain Robert Gish David H Van Thiel Zobair Younossi Myron Tong Tarek Hassanein Luis Balart Jacquelyn Fleckenstein Stephen Flamm Andres Bl | 2003 | The American Journal of Gastroenterology2003，，9： | 1 |
| 6 | Smoking increases the risk of relapse after successful tuberculosis treatment 显示文摘 | d'Arc Lyra Batista J de F6tima Pessoa Militao de Albuquerque M de Alencar Ximenes RA | 2008 | Int J Epide- miol2008，37，4： | 1 |
| 7 | Quantitative image analysis in sonograms of the thyroid gland显示文摘 | Skouroliakou C Lyra M Antoniou A | 2006 | Nuclear In-struments and Methods in Physics2006，569，2： | 1 |
| 8 | Periodic solutions and chaos in a non-linear model for the delayed cellular immune response显示文摘 | CANABARRO A A GL'ERIA I M LYRA M L | 2004 | Physica A2004，342，12： | 1 |
| 9 | Benthic cyanobacteria of the genus Nodularia are non-toxic, without gas vacuoles, able to glide and genetically more diverse than planktonic Nodularia显示文摘 | Lyra C Laamanen M Lehtimaki J M | 2005 | In- ternational journal of systematic and evolutionary microbiology2005，55，2： | 1 |
| 10 | Periodic solutions and chaos in a nonlinear model for the delayed cellular immune response显示文摘 | Canabarro A A Gleria I M Lyra M L | 2004 | Physica2004，，342： | 1 |
| 11 | Saturation effects on modulation instability in non-Kerr-like monomode optical fibers 显示文摘 | Lyra M L Gouveia--Neto A S | 1994 | Opt Commun1994，108，： | 1 |
| 12 | Quantitative image analysis in sonograms of the thyroid gland显示文摘 | Skouroliakou C Lyra M Antoniou A | 2006 | Nuclear Instruments and Methods in Physics(S0168-9002)2006，569，60： | 1 |
| 13 | Infusion of autologous bone marrow mononuclear cells through hepatic artery results in a short-term improvement of liver function in patients with chronic liver disease:apilot randomized controlled study显示文摘 | LYRA A C SOARES M B DA SILVA L F | 2010 | Eur J Gastroenterol Hepatol2010，22，： | 1 |
| 14 | Periodic so- lutions and chaos in a non-linear model for the delayed cellu- lar immune response显示文摘 | CANABARRO A A GLEERIA I M LYRA M L | 2004 | Physica A2004，34，2： | 1 |
| 15 | La erradicacio n de la peste porcina af ricana en el Brasil, 1978-1984显示文摘 | Lyra T M P | 1978 | Rev Sci rech of Int Epiz1978，，： | 1 |
| 16 | Guidelines for the prevention, diagnosis and treatment of compensatory hyperhidro- sis显示文摘 | Lyra Rde M Campos JR Kang DW | 2008 | J Bras Pneumol2008，34，11： | 1 |
| 17 | The nanl and nanj sialidases of clostridium perfringens are not essential for virulence显示文摘 | Chiarezza M Lyras D Sacha J | 2009 | Infect Immun2009，77，10： | 1 |
| 18 | A multi-objective approach to the short-term scheduling of ahydroelectric powers ystem显示文摘 | Lyra C Ferreira L R M | 1995 | IEEE Trans on Power Systems1995，10，4： | 1 |
| 19 | The natal and nanj siali- dases of clostridium perfringens are not essential for virulence 显示文摘 | Chiareza M Lyras D Sacha J et at | 2009 | Infect Immun2009，77，10： | 1 |
| 20 | Nonextensivity and mul- tifractality in low-dimensional dissipative systems 显示文摘 | LYRA M L TSALLIS C | 1998 | PhysRevLett1998，80，1： | 1 |
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