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您的检索式：作者名="Lizhou FENG"
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| 1 | Monitoring of regional drug abuse through wastewater-based epidemiology—A critical review显示文摘Wastewater-based epidemiology is a new approach to monitor drug abuse. It involves collecting wastewater, analysis of residues of drugs or its metabolites in wastewater, and back-calculation of drug consumption by taking into account wastewater flow, stability of drug target residues in wastewater, and excretion rates of drugs/metabolites. Wastewater-based epidemiology has the advantages of being inexpensive and yielding more consistent and near real-time results. It has the great potential to supplement the existing drug monitoring methods. It can be used to build large-scale(regional, national, or even continental) monitoring networks that would yield spatial patterns and temporal trends in drug abuse. This paper described in detail the principle and procedures of this wastewater-based approach. Application of this approach across the globe was also reviewed. The uncertainties involved in the approach and knowledge gaps were identified. Finally, necessity, benefits, and feasibility to set up nation or province-wide monitoring networks based on wastewater analysis in China were discussed. | Lizhou FENG Wei ZHANG Xiqing LI | 2018 | Science China Earth Sciences2018，61，3： | 2 |
| 2 | c-Met-targeted chimeric antigen receptor T cells inhibit hepatocellular carcinoma cells in vitro and in vivo显示文摘c-Met is a hepatocyte growth factor receptor overexpressed in many tumors such as hepatocellular carcinoma(HCC).Therefore,c-Met may serve as a promising target for HCC immunotherapy.Modifying T cells to express c-Met-specific chimeric antigen receptor(CAR)is an attractive strategy in treating c-Met-positive HCC.This study aimed to systematically evaluate the inhibitory effects of 2^(nd)-and 3^(rd)-generation c-Met CAR-T cells on hepatocellular carcinoma(HCC)cells.Here,2^(nd)-and 3^(rd)-generation c-Met CARs containing an anti-c-Met singlechain variable fragment(scFv)as well as the CD28 signaling domain and CD3ζ(c-Met-28-3ζ),the CD137 signaling domain and CD3ζ(c-Met-137-3ζ),or the CD28 and CD137 signaling domains and CD3ζ(c-Met-28-137-3ζ)were constructed,and their abilities to target c-Met-positive HCC cells were evaluated in vitro and in vivo.All c-Met CARs were stably expressed on T cell membrane,and c-Met CAR-T cells aggregated around c-Met-positive HCC cells and specifically killed them in vitro.c-Met-28-137-3ζCAR-T cells secreted more interferon-gamma(IFN-γ)and interleukin 2(IL-2)than c-Met-28-3ζCAR-T cells and c-Met-137-3ζCAR-T cells.Compared with c-Met low-expressed cells,c-Met CAR-T cells secreted more cytokines when co-cultured with c-Met high-expressed cells.Moreover,c-Met-28-137-3ζCAR-T cells eradicated HCC more effectively in xenograft tumor models compared with the control groups.This study suggests that 3^(rd)-generation c-Met CAR-T cells are more effective in inhibiting c-Met-positive HCC cells than 2^(nd)-generation c-Met CAR-T cells,thereby providing a promising therapeutic intervention for c-Met-positive HCC. | Xiaochen Huang Jiaojiao Guo Tao Li Lizhou Jia Xiaojun Tang Jin Zhu Qi Tang Zhenqing Feng | 2022 | The Journal of Biomedical Research2022，36，1： | 0 |
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