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1New generation of multi-scale NWP system (GRAPES):general scientific design显示文摘A new generation of numerical prediction system GRAPES (a short form of Global/Regional Assimilation and PrEdiction System) was set up in China Meteorological Administration (CMA). This paper focuses on the scientific design and preliminary results of the numerical prediction model in GRAPES, including basic idea and strategy of the general scientific design, multi-scale dynamic core, physical package configuration, architecture and parallelization of the codes. A series of numerical experiments using the real data with horizontal resolutions from 10 to 280 km and idealized experiments with very high resolution up to 100 m are conducted, giving encouraging results supporting the multi-scale application of GRAPES. The results of operational implementation of GRAPES model in some NWP centers are also presented with stress at evaluations of the capability to predict the main features of precipitation in China. Finally the issues to be dealt with for further development are discussed.CHEN DeHui XUE JiShan YANG XueSheng ZHANG HongLiang SHEN XueShun HU JiangLin WANG Yu JI LiRen CHEN JiaBin 2008Chinese Science Bulletin2008,53,22:70
2HIF-1α-induced expression of m6A reader YTHDF1 drives hypoxia-induced autophagy and malignancy of hepatocellular carcinoma by promoting ATG2A and ATG14 translation显示文摘N6-methyladenosine(m6A),and its reader protein YTHDF1,play a pivotal role in human tumorigenesis by affecting nearly everystage of RNA metabolism.Autophagy activation is one of the ways by which cancer cells survive hypoxia.However,the possibleinvolvement of m6A modification of mRNA in hypoxia-induced autophagy was unexplored in human hepatocellular carcinoma(HCO).In this study,specific variations in YTHDF1 expression were detected in YTHDF1-overexpressing,knockout,and-knockdownHCC cells,HCC organoids,and HCC patient-derived xenograft(PDX)murine models.YTHDF1 expression and hypoxia inducedautophagy were significantly correlated in vitro;signifhcant overexpression of YTHDF1 in HCC tissues was associated with poorprognosis,Multivariate cox regression analysis identihed YTHDF1 expression as an independent prognostic factor in patients withHCC.Multiple HC models conhrmed that YTHDF1 deficiency inhibited HCC autophagy,growth,and metastasis.Luciferase reporterassays and chromatin immunoprecipitation demonstrated that HlIF-1a regulated YTHDF1 transcription by directly binding to itspromoter region under hypoxia.The results of methylated RNA immunoprecipitation sequencing,proteomics,and polysomeprofling indicated that YTHDF1 contibuted to the translation of autophagy-related genes ATG2A and ATG14 by binding to m6A-modifhed ATG2A and ATG14 mRNA,thus facilitating autophagy and autophagy-related malignancy of HCC.Taken together,HlE-1d-induced YTHDF1 expression was associated with hypoxia-induced autophagy and autophagy-related HCC progression via promoting translation of autophagy-related genes ATG2A and ATG14 in a m6A-dependent manner.Our fndings suggest thatYTHDF1 is a potential prognostic biomarker and therapeutic target for patients with HCC.Qing Li Yong Ni Liren Zhang Runqiu Jiang Jing Xu Hong Yang Yuanchang Hu Jiannan Qiu Liyong Pu Jinhai Tang Xuehao Wang 2021Signal Transduction and Targeted Therapy2021,6,3:24
3Features of the EAP events on the medium-range evolution process and the mid-and high-latitude Rossby wave activities during the Meiyu period显示文摘In this paper,features for the evolution of the East Asia/Pacific(EAP) events and their association with high-and mid-latitude Rossby waves during the Meiyu period are analyzed on the medium-range time scale.It is shown that life cycles of the positive and negative EAP events cannot be simply regarded as 'mirror' each other.In the upper troposphere,downward propagations of Rossby wave packets both over high-and mid-latitude regions of Eurasian continent and over the Asian jet region are responsible for generating basic patterns of high-and mid-latitude anomaly centers of the events.In this layer,Rossby wave packets also propagate from the mid-latitude anomaly center to the high-latitude one.In the middle and lower troposphere,the formation of the subtropical anomaly center of the event is mainly attributed to the anomalous convective activity in the tropical Pacific warm pool.The northward Rossby wave energy dispersion from this center is favorable to the enhancement and maintenance of the mid-latitude anomaly center in the same layer.Finally,it might be hypothesized that typical features of the positive and negative EAP events in their mature phase result from the interaction between(or phase-locking of) respective anomalous circulations induced both by quasi-zonal Rossby wave packets embedded in upper troposphere westerly and by quasi-meridional Rossby wave packets in the background flow of the East Asian summer monsoon in the middle and lower troposphere.BUEH Cholaw SHI Ning JI LiRen WEI Jie TAO ShiYan 2008Chinese Science Bulletin2008,53,4:23
4Reactivation of γ-globin expression through Cas9 or base editor to treat β-hemoglobinopathies显示文摘Dear Editor,Mutations in the β-globin gene,the essential component of adult hemoglobin(HbA;a2p2),results in either a production of aberrant sickle hemoglobin(HbS)leading to sickle cell disease(SCD)or an insufficient β-globin synthesis leading to β-thalassemia.These two major forms of β-hemoglobinopathies cause impaired erythropoiesis and life-threatening anemia.Clinical evidence has suggested that reaaivation of fetal γ-globin(HBG)gene expression which is normally silenced after birth by certain genetic mutations can ameliorate the clinical course of β-hemoglobinopathies.Liren Wang Linxi Li Yanlin Ma Handan Hu Qi Li Yang Yang Wenbang Liu Shuming Yin Wei Li Bin Fu Ryo Kurita Yukio Nakamura Mingyao Liu Yongrong Lai Dali Li 2020Cell Research2020,30,3:14
5Verification of GRAPES unified global and regional numerical weather prediction model dynamic core显示文摘During the past few years, most of the new developed numerical weather prediction models adopt the strategy of multi-scale technique. Therefore, China Meteorological Administration has devoted to developing a new generation of global and regional multi-scale model since 2003. In order to validate the performance of the GRAPES (Global and Regional Assimilation and PrEdiction System) model both for its scientific design and program coding, a suite of idealized tests has been proposed and conducted, which includes the density flow test, three-dimensional mountain wave and the cross-polar flow test. The density flow experiment indicates that the dynamic core has the ability to simulate the fine scale nonlinear flow structures and its transient features. While the three-dimensional mountain wave test shows that the model can reproduce the horizontal and vertical propagation of internal gravity waves quite well. Cross-polar flow test demonstrates the rationality of both for the semi-Lagrangian departure point calculation and the discretization of the model near the poles. The real case forecasts reveal that the model has the ability to predict the large-scale weather regimes in summer such as the subtropical high, and to capture the major synoptic patterns in the mid and high latitudes.YANG XueSheng HU JiangLin CHEN DeHui ZHANG HongLiang SHEN XueShun CHEN JiaBin JI LiRen 2008Chinese Science Bulletin2008,53,22:14
6Increasing targeting scope of adenosine base editors in mouse and rat embryos through fusion of TadA deaminase with Cas9 variants显示文摘Lei Yang Xiaohui Zhang Liren Wang Shuming Yin Biyun Zhu Ling Xie Qiuhui Duan Huiqiong Hu Rui Zheng Yu Wei Liangyue Peng Honghui Han Jiqin Zhang Wenjuan Qiu Hongquan Geng Stefan Siwko Xueli Zhang Mingyao Liu Dali Li 2018Protein & Cell2018,9,9:13
7CRISPR/Cas9 system: a powerful technology for in vivo and ex vivo gene therapy显示文摘CRISPR/Cas9 is a versatile genome-editing tool which is widely used for modifying the genome of both prokaryotic and eukaryotic organisms for basic research and applications. An increasing number of reports have demonstrated that CRISPR/Cas9-mediated genome editing is a powerful technology for gene therapy. Here, we review the recent advances in CRISPR/Cas9-mediated gene therapy in animal models via different strategies and discuss the challenges as well as future prospects.Xiaohui Zhang Liren Wang Mingyao Liu Dali Li 2017Science China(Life Sciences)2017,60,5:12
8Single-shot real-time femtosecond imaging of temporal focusing显示文摘While the concept of focusing usually applies to the spatial domain,it is equally applicable to the time domain.Realtime imaging of temporal focusing of single ultrashort laser pulses is of great significance in exploring the physics of the space–time duality and finding diverse applications.The drastic changes in the width and intensity of an ultrashort laser pulse during temporal focusing impose a requirement for femtosecond-level exposure to capture the instantaneous light patterns generated in this exquisite phenomenon.Thus far,established ultrafast imaging techniques either struggle to reach the desired exposure time or require repeatable measurements.We have developed single-shot 10-trillion-frame-per-second compressed ultrafast photography(T-CUP),which passively captures dynamic events with 100-fs frame intervals in a single camera exposure.The synergy between compressed sensing and the Radon transformation empowers T-CUP to significantly reduce the number of projections needed for reconstructing a high-quality three-dimensional spatiotemporal datacube.As the only currently available real-time,passive imaging modality with a femtosecond exposure time,T-CUP was used to record the first-ever movie of nonrepeatable temporal focusing of a single ultrashort laser pulse in a dynamic scattering medium.T-CUP’s unprecedented ability to clearly reveal the complex evolution in the shape,intensity,and width of a temporally focused pulse in a single measurement paves the way for single-shot characterization of ultrashort pulses,experimental investigation of nonlinear light-matter interactions,and real-time wavefront engineering for deep-tissue light focusing.Jinyang Liang Liren Zhu Lihong V.Wang 2018Light(Science & Applications)2018,7,1:11
9Identification of an AFLP marker linked to the stripe rust resistance gene Yr10 in wheat显示文摘AFLP analysis of near-isogenic lines of the stripe rust resistance gene Yr10 was carried out with 6 Pst I -primers and 10 Taq I -primers with the donor parent of Yr1O gene as the check. A total of about 4200 distinguishable bands were amplified, of which 5 were stable. The genetic linkage of the 5 polymorphic DNA fragments with the target gene were tested preliminarily on a segregating F2 population derived from a cross between the gene donor parent 'Moro' and susceptible cultivar 'Mingxian 169'. The DNA fragment PT0502 was found closely linked to the Yr10 gene and cloned and sequenced. Based on the sequence specific primers for PCR were designed and synthesized. Genetic linkage analysis with 195 segregating F2 plants indicated that the genetic distance was 0.5 cM between the main product SC200 fragment produced by PCR with the primers and the Yr10 gene. The primers can be used to detect the Yr10 gene quickly, effectively and exactly.SHAO Ymgtian NIU Yongchun ZHU Lihuang ZHAI Wenxue XU Shichang WU Liren 2001Chinese Science Bulletin2001,46,17:9
10Anomalous activity of East Asian winter monsoon and the tropical Pacific SSTA显示文摘The relationship between the anomalous East Asian winter monsoon (EAWM) activity and the tropical Pacific SST anomalies has been identified using the results of 40-year integration of the IAP CGCM1 model and 10-year observational data, In the strong EAWM year, the western and central Pacific are dominated by positive SST anomalies while the eastern Pacific is negative ones. In the weak EAWM year, the SSTA pattern is quite different and shows El Nio-like SSTanomalies. The strong EAWM activity tends to create extra easterly flow to the east and extra westerly flow to the west of the warm SSTA region over the equatorial western and central Pacific, thus leading to the enhancement of convergence and convection of the flow in this region and favorable to the maintenance and development of such an SSTA pattern. On the other hand, the warm SST anomaly over the western and central Pacific, as a forcing, may lead to a specific pattern of the northern extratropical atmosphere, which is favorable to the strongCholaw Bueh Liren Ji 1999Chinese Science Bulletin1999,44,10:8
11Gene editing and its applications in biomedicine显示文摘The steady progress in genome editing, especially genome editing based on the use of clustered regularly interspaced short palindromic repeats(CRISPR) and programmable nucleases to make precise modifications to genetic material, has provided enormous opportunities to advance biomedical research and promote human health. The application of these technologies in basic biomedical research has yielded significant advances in identifying and studying key molecular targets relevant to human diseases and their treatment. The clinical translation of genome editing techniques offers unprecedented biomedical engineering capabilities in the diagnosis, prevention, and treatment of disease or disability. Here, we provide a general summary of emerging biomedical applications of genome editing, including open challenges. We also summarize the tools of genome editing and the insights derived from their applications, hoping to accelerate new discoveries and therapies in biomedicine.Guanglei Li Xiangyang Li Songkuan Zhuang Liren Wang Yifan Zhu Yangcan Chen Wen Sun Zeguang Wu Zhuo Zhou Jia Chen Xingxu Huang Jin Wang Dali Li Wei Li Haoyi Wang Wensheng Wei 2022Science China(Life Sciences)2022,65,4:8
12Wintertime extreme precipitation event over southern China and its typical circulation features显示文摘This study objectively defined an extreme precipitation event(EPE)over southern China and investigated the associated typical circulation pattern on the intraseasonal time scale.The occurrence of the EPE resulted from the joint operations of anomalous circulations over the subtropics and mid-high latitudes.During the EPE,simultaneous enhancements of the trough over the Bay of Bengal(BBT)and the Western Pacific subtropical high(WPSH)facilitated the abundant water vapor conditions over southern China,whereas the weakened East Asian major trough(EAT)led to a moderate cold air invasion to that region,producing a persistent convergence zone over southern China.Wave train pattern in North Atlantic and Europe may be viewed as precursory signals for the EPE over southern China,and it contributed to the Rossby wave propagation in association with the EPE.These wave packets propagated toward East Asia mainly along the North African-Asian subtropical westerly jet waveguide.Low-frequency disturbances along this waveguide contributed to the enhancement of the BBT and WPSH and the weakening of the EAT,constituting favorable circulation conditions for the EPE.Haifeng Zong Cholaw Bueh Liren Ji 2014Chinese Science Bulletin2014,59,10:7
13Essential role of the CUL4B ubiquitin ligase in extra-embryonic tissue development during mouse embryogenesis显示文摘CUL4B ubiquitin ligase 基因的变化有原因地被连接到 syndromic X 连接智力迟钝(XLMR ) 。然而,在 neuronal 和发展缺点的 CUL4B 变化的病原的角色没被理解。我们与 Cul4b 的指向的混乱产生了老鼠,并且与显著生长抑制观察了胚胎的致命性并且在胚胎外的纸巾增加了 apoptosis。Cul4b,然而并非它的 paralog Cul4a,在胚胎外的纸巾驿站培植在高水平被表示。在一根胚胎外的房间线的 CUL4B 表示的 Silencing 导致了 CUL4 底层 p21 Cip1/WAF 和 G2/M 房间周期逮捕 Cip1/WAF 。Cul4b 的 Epiblast 特定的删除阻止了胚胎的致命性并且产生了可行 Cul4b 空老鼠。因此,当时在胚胎非必需合适, Cul4b 在胚胎外的纸巾执行一个必要发展角色。我们的学习提供策略产生可行 Cul4b 缺乏的老鼠为潜在的 neuronal 和人的 CUL4B XLMR 病人的行为的缺乏建模。Liren Liu Yan Yin Yuewei Li Lisa Prevedel Elizabeth H Lacy Liang Ma Pengbo Zhou 2012Cell Research2012,22,8:6
14Design and Analysis of Biomimetic Nose Cone for Morphing of Aerospace Vehicle显示文摘当穿透并且重入空气时, Morphing 能力绝对是重要的让太空车辆获得气体动力学,活动性和飞行控制的占优势的功能。然而,为存在太空车辆的挑战尚待活泼地改变它鼻子锥的结构。这份报纸在由调整它的生物形态的形状提高蜜蜂的飞行特征的蜜蜂腹部上执行很多观察实验。morphing 结构从蜜蜂腹部被采用改进轴的可伸缩性和太空车辆的弯曲性质,它能导致超级演习的飞行性能。与最佳设计和拓扑学的方法结合了,新仿生的 morphing 结构被建议并且适用于太空车辆的 morphing 鼻子锥的设计。而且,模拟被进行优化 morphing 鼻子锥的结构的参数。biomimetic 鼻子锥的这个概念图案将提供一个有效方法让太空车辆减少空气动力学拖。Jieliang Zhao Shaoze Yan Liren Deng He Huang Yueming Liu 2017Journal of Bionic Engineering2017,14,2:6
15Total mesorectal excision with or without preoperative chemoradiotherapy for resectable mid/low rectal cancer: a long-term analysis of a prospective, single-center, randomized trial显示文摘Background:The preliminary results of our phase II randomized trial reported comparable functional sphincter pres-ervation rates and short-term survival outcomes between patients undergoing total mesorectal excision(TME)with or without preoperative concurrent chemoradiotherapy(CCRT).We now report the long-term results after a median follow-up of 71 months.Methods:Between March 23,2008 and August 2,2012,192 patients with T3-T4 or node-positive,resectable,mid/low rectal adenocarcinoma were randomly assigned to receive TME with or without preoperative CCRT.The following endpoints were assessed:cumulative rates of local recurrence and distant metastasis,disease-free survival(DFS),and overall survival(OS).Results:The data of 184 eligible patients were analyzed:94 patients in the TME group and 90 patients in the CCRT+TME group.In the whole cohort,the 5-year DFS and OS rates were 84.8%and 85.1%,respectively.The 5-year DFS rates were 85.2%in the CCRT+TME group and 84.3%in the TME group(P=0.969),and the 5-year OS rates were 83.5%in the CCRT+TME group and 86.5%in the TME group(P=0.719).The 5-year cumulative rates of local recur-rence were 6.3%and 5.0%(P=0.681),and the 5-year cumulative rates of distant metastasis were 15.0%and 15.7%(P=0.881)in the CCRT+TME and TME groups,respectively.No significant improvements in 5-year DFS and OS were observed with CCRT by subgroup analyses.Conclusions:Both treatment strategies yielded similar long-term outcomes.A selective policy towards preoperative CCRT is thus recommended for rectal cancer patients if high-quality TME surgery and enhanced chemotherapy can be performed.Fulong Wang Wenhua Fan Jianhong Peng Zhenhai Lu Zhizhong Pan Liren Li Yuanhong Gao Hui Li Gong Chen Xiaojun Wu Peirong Ding Zhifan Zeng Desen Wan 2018Cancer Communications2018,38,1:6
16Bleaching effect and nonvolatile holographic storage in doubly doped LiNbO_3:Fe:Cu crystals显示文摘The bleaching effect, i.e. the crystal shows that decoloration after it is illuminated by ultraviolet light, has been observed in congruent LiNbO3:Fe:Cu crystals. Based on this bleaching effect, a new technique including the recording phase by two interfering red beams and fixing phase by both UV light and a coherent red beam has been experimentally investigated to realize nonvolatile holographic storage in LiNbO3:Fe:Cu. The results of proof-of-concept experiments confirm that bleaching effect becomes an alternative physical mechanism for nonvolatile holographic storage with high recording sensitivity and weak light-induced scattering noise.LIU Dean, LIU Liren, ZHOU Changhe, REN Liyong & LI GuangaoShanghai Institute of Optics and Fine Mechanics, Chinese Academy ofSciences, Shanghai 201800, ChinaDepartment of Physics, Nankai University, Tianjin 300071, China 2002Chinese Science Bulletin2002,47,20:3
17A roughness parameter considering joint material properties and peak shear strength model for rock joints显示文摘This study aims at proposing a reasonable roughness parameter that can reflect the peak shear strength(PSS)of rock joints.Firstly,the contribution of the asperities with different apparent dip angles to shear strength is studied.Then the shear strength of the entire joint asperities is derived.The results showed that the PSS of the entire joint asperities is proportional to a key parameter hs,which is related to the geometric character of the joint surface and the joint material properties.The parameter hsis taken as the new roughness parameter,and it is reasonable to associate the PSS with the geometric characteristics of the joint surface.Based on the new roughness parameter and shear test results of 20 sets of joint specimens,a new PSS model for rock joints is proposed.The new model is validated with the artificial joints in this paper and real rock joints in published studies.Results showed that it is suitable for different types of rock joints except for gneiss joints.The new model has the form of the Mohr-Coulomb model,which can directly reflect the relationship between the 3 D roughness parameters and the peak dilation angle.Liren Ban Weisheng Du Tianwei Jin Chengzhi Qi Xiaozhao Li 2021International Journal of Mining Science and Technology2021,31,3:3
18对可切除的中/低位直肠癌是/否进行术前放化疗的全直肠系膜切除术研究:一项前瞻性、单中心、随机试验的长期分析显示文摘背景与目的我们的Ⅱ期随机试验的初步研究结果显示,对进行全直肠系膜切除术(total mesorectal excision,TME)的患者是/否进行术前同步放化疗(concurrent chemoradiotherapy,CCRT),二者的功能性括约肌保留率和短期生存结局相近。在经过中位71个月的随访后,现报告这一长期试验的结果。方法在2008年3月23日至2012年8月2日期间,192例患有T3–T4或淋巴结阳性、可切除的中/低直肠腺癌患者被随机分为接受或不接受术前CCRT组,之后均进行TME。评估以下终点:局部复发和远处转移的累积率、无病生存(disease?free survival,DFS)和总生存(overall survival,OS)。结果对入组的184例患者资料进行分析,其中TME组有94例,CCRT+TME组有90例。在整个队列中,5年DFS和OS率分别为84.8%和85.1%。CCRT+TME组的5年DFS率为85.2%,TME组为84.3%(P=0.969);CCRT+TME组的5年OS率为83.5%,TME组为86.5%(P=0.719)。在CCRT+TME和TME组中,局部复发的5年累积率分别为6.3%和5.0%(P=0.681),远处转移的5年累积率分别为15.0%和15.7%(P=0.881)。通过亚组分析,接受CCRT未观察到5年DFS和OS的显著改善。结论两种治疗方案的长期结局相近。因此,如果可以进行高质量的TME手术和强化化疗,建议选择性地对直肠癌患者进行术前CCRT。Fulong Wang Wenhua Fan Jianhong Peng Zhenhai Lu Zhizhong Pan Liren Li Yuanhong Gao Hui Li Gong Chen Xiaojun Wu Peirong Ding Zhifan Zeng Desen Wan 2019癌症2019,38,5:2
19Nature:研究鉴定出侵袭性胰腺癌细胞及其弱点显示文摘在一项新的研究中,来自美国德州大学MD安德森癌症中心的研究人员利用患者源性肿瘤异种移植(patient—derived tumorxenograft,PDx)和模式小鼠开展一系列临床前实验,鉴定出一种门卫蛋白(gatekee Perprotein)阻止胰腺癌细胞转化为一种极具侵袭性的细胞类型,并且也发现剔除这种门卫蛋白的疗法能够阻止这些癌细胞。这些发现有助为携带这种进展迅速的治疗抵抗性的胰腺癌细胞亚群的患者开发出潜在的疗法。Genovese, Giannicola Carugo, Alessandro Tepper, James Robinson, Frederick Scott Li, Liren Svelto, Maria Nezi, Luigi Corti, Denise Minelli, Rosalba Pettazzoni, Piergiorgio Gutschner, Tony Wu, Chia-Chin Seth, Sahil Akdemir, Kadir Caner Leo, Elisabetta Amin, Samirkumar Dal Molin, Marco Ying, Haoqiang Kwong, Lawrence N. Colla, Simona Takahashi, Koichi Ghosh, Papia Giuliani, Virginia Muller, Florian Dey, Prasenjit Jiang, Shan Garvey, Jill Liu, Chang-Gong Zhang, Jianhua Heffernan, Timothy P. Toniatti, Carlo Fleming, Jason B. Goggins, Michael G. Wood, Laura D. Sgambato, Alessandro Agaimy, Abbas Maitra, Anirban Roberts, Charles W. M. Wang, Huamin Viale, Andrea DePinho, Ronald A. Draetta, Giulio F. Chin, Lynda 2017现代生物医学进展2017,17,14:2
20Persistent anomalous flows over the subtropical western Pacific insummer显示文摘The distribution of global persistent anomalies shows that the subtropical western Pacific is a key region with high_frequency occurrence of persistent anomalies. Analyses of streamfunction anomalies, OLR anomalies etc. suggest that the convective heating related to Indian monsoon might be responsible for the development of WPA. The results of numerical experiment by two linear barotropic models support the observation.ZHUANG Shiyu\+1 and JI Liren\+2 1. National Meteorological Centre, China Meteorological Administration, Beijing 100081, China 2. LASG, Institute of Atmospheric Physics, Chinese Academy of Sciences, Beijing 100080, China 1998Chinese Science Bulletin1998,43,8:2
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