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| 1 | Neuroprotective effects of Ginkgo biloba extract and GinkgoUde B against oxygen-glucose deprivation/reoxygenation and glucose injury in a new in vitro multicellular network model显示文摘 | Xiaohan Yang Tiezheng Zheng Hao Hong Nan Cai Xiaofeng Zhou Changkai Sun Liying Wu Shuhong Liu Yongqi Zhao Lingling Zhu Ming Fan Xuezhong Zhou Fengxie Jin | 2018 | Frontiers of Medicine2018，12，3： | 12 |
| 2 | Formability,microstructure evolution and mechanical properties of wire arc additively manufactured AZ80M magnesium alloy using gas tungsten arc welding显示文摘Wire arc additive manufacturing(WAAM)technology has been used to fabricate the multi-layer single-pass deposited wall of AZ80M magnesium(Mg)alloy by gas tungsten arc welding.The formability,thermal cycles,microstructural evolution and mechanical properties of the WAAM AZ80M Mg alloy were investigated.The results show that there was significant difference in the temperature variation and the geometries between the original several layers and the subsequent deposited layers.Owing to the arc energy input,the interpass temperature rised rapidly and then stabilized at 150℃.As a result,the width of the deposited wall increased and then kept stable.There were obvious differences in the microstructure of the WAAM AZ80M Mg alloy among the top zone,intermediate zone and bottom zone of deposited wall.During the arc deposition process,theβphase of the WAAM AZ80M Mg alloy redissolved due to the cyclic heat accumulation,and then precipitated in the grain boundary.The cyclic heat accumulation also led to weakening of dendrite segregation.From the substrate to the top zone,the hardness of the deposited wall decreased gradually,and the intermediate zone which was the main body of deposited wall had relatively uniform hardness.The tensile properties of the WAAM AZ80M Mg alloy were different between the vertical direction and the horizontal direction.And the maximum ultimate tensile strength of the WAAM AZ80M Mg alloy was 308 MPa which was close to that of the as-extruded AZ80M Mg alloy. | Yangyang Guo Gaofeng Quan Yinglong Jiang Lingbao Ren Lingling Fan Houhong Pan | 2021 | Journal of Magnesium and Alloys2021，9，1： | 10 |
| 3 | WIP1 Phosphatase Plays a Critical Neuroprotective Role in Brain Injury Induced by High-Altitude Hypoxic Inflammation显示文摘The hypobaric hypoxic environment in highaltitude areas often aggravates the severity of inflammation and induces brain injury as a consequence. However, the critical genes regulating this process remain largely unknown. The phosphatase wild-type p53-induced phosphatase 1(WIP1) plays important roles in various physiological and pathological processes, including the regulation of inflammation in normoxia, but its functions in hypoxic inflammation-induced brain injury remain unclear.Here, we established a mouse model of this type of injury and found that WIP1 deficiency augmented the release of inflammatory cytokines in the peripheral circulation and brain tissue, increased the numbers of activated microglia/macrophages in the brain, aggravated cerebral histological lesions, and exacerbated the impairment of motor and cognitive abilities. Collectively, these results provide the first in vivo evidence that WIP1 is a critical neuroprotector against hypoxic inflammation-induced brain injury. | Dahu Li Lijun Zhang Xin Huang Lili Liu Yunling He Lun Xu Yiyao Zhang Tong Zhao Liying Wu Yongqi Zhao Kuiwu Wu Yan Wu Ming Fan Lingling Zhu | 2017 | Neuroscience Bulletin2017，33，3： | 9 |
| 4 | 中国卫生人力资源:数量、质量和分布显示文摘在本文中,我们从数量、质量和分布的角度对中国现有的卫生人力资源进行了分析。与绝大多数国家不同,2005年中国拥有的医生总数大于护士总数——注册的执业医师和执业助理医师总数达190多万,注册的护士总数为140多万。城市地区的医生密度是农村地区的2倍多,而城市地区护士的密度是农村地区的3倍多。中国大多数医生(67.2%)和护士(97.5%)所接受的教育仅为大专或中专水平。自1998年以来,中国的医学卫生教育事业有了很大的发展,医学院校不断扩招又导致了卫生人员供过于求的局面。 | Sudhir Anand Victoria Y Fan Junhua Zhang Lingling Zhang Yang Ke Zhe Dang Lincoln C Chen | 2009 | 世界临床医学2009，3，1： | 9 |
| 5 | Sequential fate-switches in stem-like cells drive the tumorigenic trajectory from human neural stem cells to malignant glioma显示文摘Glioblastoma(GBM)is an incurable and highly heterogeneous brain tumor,originating from human neural stem/progenitor cells(hNSCs/hNPCs)years ahead of diagnosis.Despite extensive efforts to characterize hNSCs and end-stage GBM at bulk and single-cell levels,the de novo gliomagenic path from hNSCs is largely unknown due to technical difficulties in early-stage sampling and preclinical modeling.Here,we established two highly penetrant hNSC-derived malignant glioma models,which resemble the histopathology and transcriptional heterogeneity of human GBM.Integrating time-series analyses of whole-exome sequencing,bulk and single-cell RNA-seq,we reconstructed gliomagenic trajectories,and identified a persistent NSC-like population at all stages of tumorigenesis.Through trajectory analyses and lineage tracing,we showed that tumor progression is primarily driven by multi-step transcriptional reprogramming and fate-switches in the NSC-like cells,which sequentially generate malignant heterogeneity and induce tumor phenotype transitions.We further uncovered stage-specific oncogenic cascades,and among the candidate genes we functionally validated C1QL1 as a new glioma-promoting factor.Importantly,the neurogenic-to-gliogenic switch in NSC-like cells marks an early stage characterized by a burst of oncogenic alterations,during which transient AP-1 inhibition is sufficient to inhibit gliomagenesis.Together,our results reveal previously undercharacterized molecular dynamics and fate choices driving de novo gliomagenesis from hNSCs,and provide a blueprint for potential early-stage treatment/diagnosis for GBM. | Xiaofei Wang Ran Zhou Yanzhen Xiong Lingling Zhou Xiang Yan Manli Wang Fan Li Chuanxing Xie Yiming Zhang Zongyao Huang Chaoqiong Ding Kaidou Shi Weida Li Yu Liu Zhongwei Cao Zhen-Ning Zhang Shengtao Zhou Chong Chen Yan Zhang Lu Chen Yuan Wang | 2021 | Cell Research2021，31，6： | 8 |
| 6 | Evolution of the East China Sea sedimentary environment in the past 14 kyr: Insights from tetraethers-based proxies显示文摘We reconstruct the environmental evolution of the East China Sea in the past 14 kyr based on glycerol dialkyl glycerol tetraethers(GDGTs) in a sediment core from the subaqueous Yangtze River Delta. Two primary phases are recognized. Phase I(13.8–8 cal kyr BP) reflects a predominantly continental influence, showing distinctly higher concentrations of branched GDGTs(averaged 143 ng/g dry sediment weight, dsw) than isoprenoid GDGTs(averaged 36 ng/g dsw), high BIT index(branched vs. isoprenoid tetraethers) values(>0.78) and a fluctuating GDGT-0/crenarchaeol ratio(R_(0/5), varied from 0.52 to 3.81). Within this interval, temporal increases of terrestrial and marine influence are attributed to Younger Dryas(YD)(ca. 12.9–12.2 cal kyr BP) cold event and melt-water pulse(MWP)-1B(11.5–11.1 cal kyr BP), respectively. The prominent transition from 8 to 7.9 cal kyr BP shows a sharp decrease in BIT index value(<0.4) and increase in crenarchaeol, which marks the beginning of phase II. Afterwards, the proxies remain relatively constant, which indicates that phase II(7.9 cal kyr BP-present) is a shelf sedimentary environment with high stand of sea level. Overall, the BIT index in our record serves as a good marker for terrestrial influence at the site, and likely reflects the flooding history of the region. The TEX_(86)(Tetra Ether Index of tetraethers consisting of 86 carbons) proxy is not applicable in phase I because of an excess terrestrial influence; but it seems to be valid for revealing the annual SST in phase II(21.6±0.9°C, n=49). In contrast, the MBT'/CBT(Methylation of Branched Tetraethers and Cyclization of Branched Tetraethers) proxy appears to faithfully record the annual mean air temperature(MAT)(14.3±0.63°C, n=68) and presents an integrated signal over the middle and lower Yangtze River drainage basin. | GE HuangMin ZHANG ChuanLun VERSTEEGH Gerard J.M. CHEN LingLing FAN DaiDu DONG Liang LIU JingJing | 2016 | Science China Earth Sciences2016，59，5： | 6 |
| 7 | The heterogeneity of islet autoantibodies and the progression of islet failure in type 1 diabetic patients显示文摘Type 1 diabetes mellitus is heterogeneous in many facets. The patients suffered from type 1 diabetes present several levels of islet function as well as variable number and type of islet-specific autoantibodies. This study was to investigate prevalence and heterogeneity of the islet autoantibodies and clinical phenotypes of type 1 diabetes mellitus; and also discussed the process of islet failure and its risk factors in Chinese type 1 diabetic patients. A total of 1,291 type 1 diabetic patients were enrolled in this study. Demographic information was collected. Laboratory tests including mixed-meal tolerance test, human leukocyte antigen alleles, hemoglobin A1 c, lipids, thyroid function and islet autoantibodies were conducted. The frequency of islet-specific autoantibody in newly diagnosed T1 DM patients(duration shorter than half year) was 73% in East China. According to binary logistic regressions, autoantibody positivity, longer duration and lower Body Mass Index were the risk factors of islet failure. As the disease developed, autoantibodies against glutamic acid decarboxylase declined as well as the other two autoantibodies against zinc transporter 8 and islet antigen 2. The decrease of autoantibodies was positively correlated with aggressive beta cell destruction. Autoantibodies can facilitate the identification of classic T1 DM from other subtypes and predict the progression of islet failure. As there were obvious heterogeneity in autoantibodies and clinical manifestation in different phenotypes of the disease, we should take more factors into consideration when identifying type 1 diabetes mellitus. | Jin Liu Lingling Bian Li Ji Yang Chen Heng Chen Yong Gu Bingqin Ma Wei Gu Xinyu Xu Yun Shi Jian Wang Dalong Zhu Zilin Sun Jianhua Ma Hui Jin Xing Shi Heng Miao Bing Xin Yan Zhu Zhenwen Zhang Ruifang Bu Lan Xu Guangde Shi Wei Tang Wei Li Dongmei Zhou Jun Liang Xingbo Cheng Bimin Shi Jixiang Dong Ji Hu Chen Fang Shao Zhong Weinan Yu Weiping Lu Chenguang Wu Li Qian Jiancheng Yu Jialin Gao Xiaoqiang Fei Qingqing Zhang Xueqin Wang Shiwei Cui Jinluo Cheng Ning Xu Guofeng Wang Guoqing Han Chunrong Xu Yun Xie Minmin An Wei Zhang Zhixiao Wang Yun Cai Qi Fu Yu Fu Shuai Zheng Fan Yang Qingfang Hu Hao Dai Yu Jin Zheng Zhang Kuanfeng Xu Yifan Li Jie Shen Hongwen Zhou Wei He Xuqin Zheng Xiao Han Liping Yu Jinxiong She Mei Zhang Tao Yang | 2016 | Science China(Life Sciences)2016，59，9： | 5 |
| 8 | Effect of heat treatment on the microstructure and mechanical properties of AZ80M magnesium alloy fabricated by wire arc additive manufacturing显示文摘To maximize the benefits of wire arc additive manufacturing(WAAM)processes,the effect of post-deposition heat treatment on the microstructure and mechanical properties of WAAM AZ80M magnesium(Mg)alloy was investigated.Three different heat treatment procedures(T4,T5 and T6)were performed.According to the results,after T4 heat treatment,the microsegregation of alloying elements was improved with the eutectic structure dissolved.Samples after T5 heat treatment inherited the net-like distribution of secondary phases similar to the as-deposited sample,where the eutectic structure covering the interdendritic regions and theβ-phase precipitated around the eutectic structure.After T6 heat treatment,the tinyβ-phases re-precipitated from the matrix and distributed in inner and outer of the grains.The hardness distribution of the samples went through T4 and T6 heat treatment was more uniform in comparison to that of T5 heat treated samples.The tensile test showed that the T6 heat treatment improved the strength and ductility,and the anisotropy between horizontal and vertical can be eliminated.Moreover,T4 treated samples exhibited highest ductility. | Yangyang Guo Gaofeng Quan Mert Celikin Lingbao Ren Yuhang Zhan Lingling Fan Houhong Pan | 2022 | Journal of Magnesium and Alloys2022，10，7： | 5 |
| 9 | Visualization of aging-associated chromatin alterations with an engineered TALE system显示文摘在实时房间的特定的 genomic loci 的可视化在多样的生物过程期间是为在染色质建筑学的动态变化的调查的一个前提,例如细胞的老化。然而,当前的精确 genomic 成像方法被缺乏妨碍荧光灯有高特性和 signal-to-noise 的探针形成对照。我们发现那常规抄写像使活跃之物的受动器(故事) 趋于形成蛋白质总数,从而在成像应用程序损害他们的性能。通过屏蔽,我们发现有故事的那熔化 thioredoxin 阻止了总数形成,开基于故事的 genomic 成像的完整的力量。用熔化 thioredoxin 的故事(TTALE ) ,我们在各种各样的 genomic loci 完成了高质量的成像并且观察联系老化(epi ) 在在人和老鼠早衰模型的 telomeres 和着丝点的 genomic 改变。重要地,我们鉴别 ribosomal DNA 的摩擦为人的老化作为一个分子的标记重复。我们的学习为精确在 vitro 并且在 vivo 监视染色质动力学建立一个简单、柔韧的成像方法。 | Ruotong Ren Liping Deng Yanhong Xue Keiichiro Suzuki Weiqi Zhang Yang Yu Jun Wu Liang Sun Xiaojun Gong Huiqin Luan Fan Yang Zhenyu Ju Xiaoqing Ren Si Wang Hong Tang Lingling Geng Weizhou Zhang Jian Li Jie Qiao Tao Xu Jing Qu Guang-Hui Liu | 2017 | Cell Research2017，27，4： | 4 |
| 10 | Loss of Wip1 aggravates brain injury after ischaemia/reperfusion by overactivating microglia显示文摘Background and purpose The inflammatory response mediated by microglia/macrophages is closely related to cerebral ischaemia/reperfusion injury.Wild-type p53-induced protein phosphatase 1(Wip1),a serine/threonine phosphatase,is expressed in various tissues.A growing number of reports have suggested that Wip1 is a negative regulator of inflammation in peripheral tissue;however,its role in the central nervous system(CNS)remains unclear.This study aimed to clarify whether Wip1 can inhibit CNS inflammation by regulating microglia/macrophage functions after ischaemic injury.Methods A model of middle cerebral artery occlusion and reperfusion was established in mice.CNS inflammation was simulated by lipopolysaccharide treatment of primary microglia.Laser speckle imaging was used to monitor regional cerebral blood flow.Behavioural outcomes were assessed with a TreadScan gait analysis system.TTC staining was used to evaluate the infarct volume,and western blotting and immunofluorescence staining were applied to detect the phenotypical transformation of microglia.ELISA was performed to detect the levels of inflammatory factors.Results Wip1 expression was increased after ischaemia/reperfusion.Wip1-knockout(KO)mice displayed more severe brain injury than wild-type mice,as indicated by aggravated motor dysfunction,greater brain infarct volumes and higher expression of inflammatory cytokines(interleukin-6 and tumour necrosis factor alpha)in the brain.We also found that Wip1 depletion increased microglial/macrophage activation in both in vitro and in vivo models,which all showed activation of microglia/macrophages.Lentivirus-Ppm1d reversed the injury induced by Wip1-KO.Conclusions Our results suggest that Wip1 may inhibit neuroinflammation by inhibiting microglial/macrophage activation after brain ischaemia/reperfusion injury. | Feng Yan Xiang Cheng Ming Zhao Shenghui Gong Ying Han Liping Ding Di Wu Yumin Luo Wei Zuo Lingling Zhu Ming Fan Xunming Ji | 2021 | Stroke & Vascular Neurology2021，6，3： | 4 |
| 11 | Oceanic barrier layer variation induced by tropical cyclones in the Northwest Pacific显示文摘According to Argo profiles and one-dimensional Price-Weller-Pinkel models, the oceanic barrier layer variation induced by tropical cyclones is adequately analyzed in the Northwest Pacific. Results show that tropical cyclones mainly aff ect the oceanic barrier layer through intensifying and weakening pre-existed barrier layer. The former even may generate new one after tropical cyclones’ passage. The latter can make pre-existed one disappear. Local wind stress and precipitation, the dominant factors, primarily determine the variation of barrier layer. Negative eff ects of wind mainly focus on the north of 20°N. This phenomenon is more meaningful for slow tropical cyclones. Conversely, positive eff ects of wind and precipitation center on the south of 20°N in the Northwest Pacific. Some data indicate that the barrier layer variation is also closely related with initial mixed layer depth and barrier layer thickness. | ZHANG Zhixiang LIU Lingling WANG Fan | 2019 | Journal of Oceanology and Limnology2019，37，2： | 3 |
| 12 | Purification and characterization of phenoloxidase from Octopus ocellatus显示文摘从章鱼 ocellatus 的墨水囊的 Phenoloxidase (PO ) 被胶化过滤和离子交换层析净化,并且由把 L-dihydroxyphenylalanine (L-DOPA ) 用作特定的底层以它的生物化学、酶的性质描绘了。它从 O 被发现那 prophenoloxidase。当 153.8 kDa 的 heterodimeric 蛋白质,和 75.6 和 73.0 kDa 的二个子单元经常在 SDS 激活以后在准备被检测, ocellatus 被孤立。象 PO 一样活动显示出 7.0 的最佳的 pH, 40 的最佳的温度 ?????? | Tingjun Fan Mingyu Li Jing Wang Lingling Yang Rishan Cong | 2009 | Acta Biochimica et Biophysica Sinica2009，41，10： | 3 |
| 13 | Review on observational studies of western tropical Pacific Ocean circulation and climate显示文摘The Western Tropical Pacific(WTP) Ocean holds the largest area of warm water(>28℃) in the world ocean referred to as the Western Pacific Warm Pool(WPWP),which modulates the regional and global climate through strong atmospheric convection and its variability.The WTP is unique in terms of its complex 3-D ocean circulation system and intensive multiscale variability,making it crucial in the water and energy cycle of the global ocean.Great advances have been made in understanding the complexity of the WTP ocean circulation and associated climate impact by the international scientific community since the 1960 s through field experiments.In this study,we review the evolving insight to the 3-D structure and multi-scale variability of the ocean circulation in the WTP and their climatic impacts based on in-situ ocean observations in the past decades,with emphasis on the achievements since 2000.The challenges and open que stions remaining are reviewed as well as future plan for international study of the WTP ocean circulation and climate. | HU Dunxin WANG Fan SPRINTALL Janet WU Lixin RISER Stephen CRAVATTE Sophie GORDON Arnold ZHANG Linlin CHEN Dake ZHOU Hui ANDO Kentaro WANG Jianing LEE Jae-Hak HU Shijian WANG Jing ZHANG Dongxiao FENG Junqiao LIU Lingling VILLANOY Cesar KALUWIN Chalapan QU Tangdong MA Yixin | 2020 | Journal of Oceanology and Limnology2020，38，4： | 3 |
| 14 | Salivary protease spectrum biomarkers of oral cancer显示文摘Proteases are important molecules that are involved in many physiological and pathological processes of the human body,such as growth,apoptosis and metastasis cancer cells.They are potential targets in cancer diagnosis and biotherapy.In this study,we analyzed the salivary protease spectrum of patients with oral squamous cell carcinoma (OSCC),oral benign masses and chronic periodontitis,as well as that of health,using human protease array kits,enzyme-linked immunosorbent assay,western blot and immunofluorescence.The salivary protease spectrum was found to be associated with oral diseases.For example,the saliva of patients with OSCC contained increased numbers of proteases than those of other oral diseases and health.The levels of matrix metalloproteinase (MMP)-1,MMP-2,MMP-10,MMP-12,A disintegrin and metalloprotease (ADAM)9,A disintegrin and metalloprotease with thrombospondin type 13 motifs (ADAMST13),cathepsin V and kallikrein 5 in the saliva of patients with OSCC were significantly increased compared with those of other groups.Taking MMP-1,cathepsin V,kallikrein 5 and ADAM9 as biomarkers of OSCC,cutoff values were199,11.34,9.29 and 202.55 pg·mL?1,respectively.From the area under the curve,sensitivity and specificity,the combination of cathepsin V/kallikrein5/ADAM9 was an optimal biomarker for diagnosing OSCC.Thus,analysis of the salivary protease spectrum may be an innovative and cost-efficient approach to evaluating the health status of the oral cavity.Specifically,increases in cathepsin V,kallikrein 5 and ADAM9 may be useful biomarkers in the screening and diagnosis of OSCC. | Yun Feng Qian Li Jiao Chen Ping Yi Xin Xu Yaping Fan Bomiao Cui Yu Yu Xiaoying Li Yue Du Qianming Chen Lingling Zhang Jingjing Jiang Xuedong Zhou Ping Zhang | 2019 | International Journal of Oral Science2019，11，2： | 2 |
| 15 | Global and local information combined to detect singular points in fingerprint images显示文摘An algorithm is proposed,which combines global and local information of fingerprint images to detect singular points.It's mathematically proven that normal lines of gradient of double orientation field(GDOF) pass through singular points.Normal lines of GDOF use rather global information to detect candidate singular points.Fingerprint image is divided into blocks and normal lines of GDOF are drawn.The number of normal lines that pass through each block is accumulated.The block that has the maximum number corresponds to a candidate singular point.It can be seen as a kind of Hough transform(HT).As candidate singular points detected by global information may have a little warp from their real positions,it's necessary to use local information to refine their positions.Poincare index is chosen,and uses local information to refine the candidate singular points.This makes our algorithm more robust to noise than methods that only use local information.What's more,the pairs of the detected singular points are used to classify fingerprint.Experimental results show that our algorithm performs well and fast enough for real time application in databases NIST-4. | FAN LingLing WANG ShuGuang GUO TianDe | 2013 | Science China(Information Sciences)2013，56，6： | 2 |
| 16 | Dynamic features of near-inertial oscillations in the Northwestern Pacific derived from mooring observations from^2015 to 2018显示文摘Near-inertial oscillation is an important physical process transferring surface wind energy into deep ocean.We investigated the near-inertial kinetic energy(NIKE)variability using acoustic Doppler current profiler measurements from a mooring array deployed in the tropical western Pacific Ocean along 130°E at 8.5°N,11°N,12.6°N,15°N,and 17.5°N from September 2015 to January 2018.Spatial features,decay timescales,and significant seasonal variability of the observed NIKE were described.At the mooring sites of 17.5°N,15°N,and 12.6°N,the NIKE peaks occurred in boreal autumn and the NIKE troughs were observed in boreal spring.By contrast,the NIKE at 11°N and 8.5°N showed peaks in winter and troughs in summer.Tropical cyclones and strong wind events played an important role in the emergence of high-NIKE events and explained the seasonality and latitudinal characteristics of the observed NIKE. | HU Shijian LIU Lingling GUAN Cong ZHANG Linlin WANG Jianing WANG Qingye MA Jie WANG Fujun JIA Fan FENG Junqiao LU Xi WANG Fan HU Dunxin | 2020 | Journal of Oceanology and Limnology2020，38，4： | 2 |
| 17 | RNA modifications:importance in immune cell biology and related diseases显示文摘RNA modifications have become hot topics recently.By influencing RNA processes,including generation,transportation,function,and metabolization,they act as critical regulators of cell biology.The immune cell abnormality in human diseases is also a research focus and progressing rapidly these years.Studies have demonstrated that RNA modifications participate in the multiple biological processes of immune cells,including development,differentiation,activation,migration,and polarization,thereby modulating the immune responses and are involved in some immune related diseases.In this review,we present existing knowledge of the biological functions and underlying mechanisms of RNA modifications,including N6-methyladenosine(m^(6)A),5-methylcytosine(m^(5)C),N1-methyladenosine(m1 A),N7-methylguanosine(m^(7)G),N4-acetylcytosine(ac^(4)C),pseudouridine(Ψ),uridylation,and adenosine-to-inosine(A-to-I)RNA editing,and summarize their critical roles in immune cell biology.Via regulating the biological processes of immune cells,RNA modifications can participate in the pathogenesis of immune related diseases,such as cancers,infection,inflammatory and autoimmune diseases.We further highlight the challenges and future directions based on the existing knowledge.All in all,this review will provide helpful knowledge as well as novel ideas for the researchers in this area. | Lian Cui Rui Ma Jiangluyi Cai Chunyuan Guo Zeyu Chen Lingling Yao Yuanyuan Wang Rui Fan Xin Wang Yuling Shi | 2022 | Signal Transduction and Targeted Therapy2022，7，10： | 2 |
| 18 | Reactive oxygen species in paraventricular nucleus involved in cardiac sympathetic afferent reflex in rats显示文摘Objective: The present study was designed to determine if reactive oxygen species (ROS) in the paraventricular nucleus (PVN) were involved in modulating cardiac sympathetic afferent reflex (CSAR) in anesthetized rats. Methods: Malondialdehyde (MDA), the end product of lipid peroxidation, in the PVN, was determined by thiobarbituric acid (TBA) spectrometric method. Renal sympathetic nerve activity (RSNA) and arterial pressure were recorded in sinoaortic-denervated and cervical-vagotomized rats. The CSAR was evaluated by the response of the RSNA evoked by epicardial application of bradykinin (BK, 0.4 μg). Results: The MDA in the PVN was significantly increased after epicardial application of BK compared with control (2.0±0.3 vs 0.8±0.1 nmol/mg protein, P<0.01). Microinjection of a superoxide anion scavenger, tiron (20 nmol) into the PVN significantly inhibited the CSAR evoked by BK (12.3±1.9 vs 4.2±1.2%, P<0.01) and decreased MDA level (1.9±0.3 vs 0.6±0.1 nmol/mg protein, P<0.01) compared with control. Conclusion: The ROS in the PVN is involved in modulating the CSAR in rats. | Feng Zhang Yang Yu Ying Zhang Yingchun Li Luqing Zhang Lingling Fan Yingya Gao Guoqing Zhu | 2005 | Journal of Nanjing Medical University2005，19，6： | 2 |
| 19 | Upregulation of GLT25D1 in Hepatic Stellate Cells Promotes Liver Fibrosis via the TGF-β1/SMAD3 Pathway In Vivo and In vitro显示文摘Background and Aims:Collagenβ(1-O)galactosyltransferase 25 domain 1(GLT25D1)is associated with collagen production and glycosylation,and its knockout in mice results in embryonic death.However,its role in liver fibrosis remains elusive,particularly in hepatic stellate cells(HSCs),the primary collagen-producing cells associated with liver fibrogenesis.Herein,we aimed to elucidate the role of GLT25D1 in HSCs.Methods:Bile duct ligation(BDL)-induced mouse liver fibrosis models,primary mouse HSCs(mHSCs),and transforming growth factor beta 1(TGF-β1)-stimulated LX-2 human hepatic stellate cells were used in in vivo and in vitro studies.Stable LX-2 cell lines with either GLT25D1 overexpression or knockdown were established using lentiviral transfection.RNA-seq was performed to investigate the genomic differences.HPLCMS/MS were used to identify glycosylation sites.Scanning electronic microscopy(SEM)and second-harmonic generation/two-photon excited fluorescence(SHG/TPEF)were used to image collagen fibril morphology.Results:GLT25D1 expression was upregulated in nonparenchymal cells in human cirrhotic liver tissues.Meanwhile,its knockdown attenuated collagen deposition in BDL-induced mouse liver fibrosis and inhibited mHSC activation.GLT25D1 was overexpressed in activated versus quiescence LX-2 cells and regulated in vitro LX-2 cell activation,including proliferation,contraction,and migration.GLT25D1 also significantly increased liver fibrogenic gene and protein expression.GLT25D1 upregulation promoted HSC activation and enhanced collagen expression through the TGF-β1/SMAD signaling pathway.Mass spectrometry showed that GLT25D1 regulated the glycosylation of collagen in HSCs,affecting the diameter of collagen fibers.Conclusions:Collectively,the upregulation of GLT25D1 in HSCs promoted the progression of liver fibrosis by affecting HSCs activation and collagen stability. | Shiwei Wang Lingling He Fan Xiao Meixin Gao Herui Wei Junru Yang Yang Shu Fuyang Zhang Xiaohui Ye Ping Li Xiaohua Hao Xingang Zhou Hongshan Wei | 2023 | Journal of Clinical and Translational Hepatology2023，11，1： | 1 |
| 20 | Wind farms with HVDC delivery in inertial response and primary frequency control显示文摘 | MIAO Zhixin FAN Lingling OSHBORN D | 2010 | IEEE Transactions on Energy Conversions2010，25，4： | 1 |
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