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| 1 | CIMMYT人工合成小麦与普通小麦杂交后代籽粒硬度puroindoline基因等位变异检测显示文摘【目的】山羊草与硬粒小麦杂交培育的人工合成小麦已广泛应用于国内外小麦品种改良,研究人工合成小麦与普通小麦杂交后代的籽粒硬度变异类型,有助于提高育种效率。【方法】以来自CIMMYT的176份人工合成小麦与普通小麦杂交后代为材料,采用单籽粒谷物硬度测试仪、特异引物的PCR扩增和改进的SDS-PAGE凝胶电泳对其SKCS硬度及其基因型进行了研究。【结果】硬质基因型均为Pina-D1b/Pinb-D1a类型,软质基因型有Pina-D1a/Pinb-D1a、Pina-D1j/Pinb-D1i、Pina-D1a/Pinb-D1i和Pina-D1c/Pinb-D1h等4种类型。其中Pina-D1j/Pinb-D1i是最常见的突变类型,占软质麦总数的49.1%。不同puroindoline类型的硬度比较表明,Pina-D1b/Pinb-D1a与其它类型间差异均达1%显著水平。在软质基因型中,Pina-D1c/Pinb-D1h和Pina-D1a/Pinb-D1i类型硬度相对较高,与Pina-D1j/Pinb-D1i和Pina-D1a/Pinb-D1a的籽粒硬度差异达5%显著水平。另外,Pina-D1a/Pinb-D1i的千粒重和粒径也显著高于其它变异类型。【结论】本试验发现了多种普通小麦中未曾发现过的puroindoline基因型,且硬度数值间存在显著差异,为人工合成小麦在品种改良中的应用和我国从CIMMYT引种提供了依据,同时也有助于puroindoline基因的多态性研究。 | 陈锋 夏先春 王德森 Morten Lillemo 何中虎 | 2006 | 中国农业科学2006，39，3： | 27 |
| 2 | CIMMYT普通小麦籽粒硬度等位变异的检测显示文摘籽粒硬度主要由5D染色体短臂的1对主效基因Ha控制,研究籽粒硬度等位变异有助于提高小麦的磨粉和食品加工品质。本试验对国际玉米小麦改良中心(CIMMYT)的138份历史品种和代表性高代品系的硬度基因型进行了研究。在用SDS-PAGE鉴定Pina-D1 b/Pinb-D1 a时,用10%甘油代替水配制分离胶,用PDA代替甲叉配制分离胶和浓缩胶,增强了PINA和PINB两种蛋白带型的分辨率。结果表明,与其他国家硬质麦中Pina-D1 a/Pinb-D1 b类型偏多的特点明显不同,CIMMYT硬质小麦中puroindoline a(PINA)蛋白缺失类型(或称Pina-D1 b/Pinb-D1 a)较多,为118个,占85.5%;Pina-D1 a/Pinb-D1 a(野生型)为11个,占8.0%;Pina-D1 a/Pinb-D1 b类型有9个,占6.5%。其中,PINA缺失对小麦籽粒硬度的影响最大,与其他2种基因型硬度值之间差异达5%显著水平。先前研究结果表明,PINA蛋白缺失类型的磨粉品质和面包烘烤品质均劣于Pina-D1 a/Pinb-D1 b类型,因此建议CIMMYT多引进一些其他硬度变异类型的小麦种质,如Pina-D1 a/Pinb-D1 b类型等,以改善其硬度基因型过度单一的局面,从而减少PINA蛋白缺失带来的不利影响。同时,也提醒我国以其他用途如抗病、抗旱等为目的引种CIMMYT小麦时,还应充分考虑PINA蛋白缺失对磨粉和加工品质的不利影响,以更合理引进和有效利用CIMMYT种质资源。 | 陈锋 何中虎 Morten Lillemo 夏先春 | 2005 | 作物学报2005，31，10： | 18 |
| 3 | 鲁麦21慢白粉病抗性基因数目和遗传力分析显示文摘以多年鉴定具有慢白粉抗性的小麦品种鲁麦21和感白粉病品种京双16及其杂交组合F2:3和F2:4代株系200个为材料,于2005—2007年连续2个生长季,在北京和安阳两地分别进行田间病害鉴定,并采用质量性状和数量性状2种分析方法估算鲁麦21的慢病基因数目和遗传力。结果表明,在这2个群体中至少存在4对抗性基因,其广义遗传力为0.53~0.78。由于出现超亲分离,因此推测京双16可能贡献1对微效抗病基因,而鲁麦21至少含有3对慢白粉病抗性基因。 | 倪小文 阎俊 陈新民 夏先春 何中虎 张勇 王德森 Morten Lillemo | 2008 | 作物学报2008，34，8： | 10 |
| 4 | CIMMYT小麦品种Saar的叶锈成株抗性QTL分析显示文摘【目的】小麦品种Saar由CIMMYT育成,在欧洲、亚洲和南美洲对小麦叶锈、条锈和白粉病均表现出很高的成株抗性,发掘其成株抗叶锈QTL对于选育持久抗锈品种有重要作用。【方法】以Avocet与Saar杂交的109个F6代重组自交系为材料,利用142个SSR标记和209DArT(Diversity Arrays Technology)标记构建连锁图,对Saar和Avocet的成株抗性进行QTL分析。试验材料于2006-2007年度种植在河北保定和河南新乡两个试验点,调查各个家系对叶锈病的成株抗性。【结果】由351个位点组成的遗传连锁图,覆盖小麦21个连锁群,全长3083cM。采用复合区间作图法进行叶锈成株抗性的QTL分析,在1BL、2DS、5BL、6AL和7DS染色体上发现了5个抗叶锈病QTL,分别解释4.5%~6.4%、12.2%~12.5%、4.9%~11.2%、4.9%~7.8%和14.0%~67.6%的表型变异。【结论】叶锈成株抗性基因及其紧密连锁分子标记的发掘,将为小麦抗叶锈病育种的分子标记辅助选择(MAS)提供理论和技术支持。 | 张利军 李在峰 Morten Lillemo 夏先春 刘大群 杨文香 罗家传 王海燕 | 2009 | 中国农业科学2009，42，2： | 9 |
| 5 | CIMMYT小麦puroindoline基因型的进一步鉴定与分析显示文摘目的籽粒硬度是小麦市场分级和定价的重要依据,影响磨粉和食品加工品质。CIMMYT是我国最重要小麦引种地之一,研究其硬度基因型对我国小麦引种具有重要的应用价值。方法以CIMMYT常用的236份小麦亲本和高代品系为材料,采用改进的颗粒指数法、特异引物的PCR扩增和改进的SDS-PAGE凝胶电泳对其SKCS硬度及其puroindoine基因型进行鉴定和分析。结果202份硬质麦中,165份为PINA蛋白缺失类型;37份为Pinb-D1b类型,其中19份为非CIMMYT材料,18份亲本中均含有非CIMMYT材料。在34份软质麦中,有野生型、Pina-D1a/Pinb-D1i、Pina-D1c/Pinb-D1h、Pina-D1j/Pinb-D1i和Pina-D1a/Pinb-D1j共5种基因型。两种硬质类型中,Pina-D1b类型胚乳硬质程度明显高于(PSI硬度显著低于)Pinb-D1b类型;4种软质类型中,Pina-D1c/Pinb-D1h和Pina-D1a/Pinb-D1i类型胚乳硬质程度显著高于(PSI硬度显著低于)Pina-D1j/Pinb-D1i和Pina-D1a/Pinb-D1a类型。通过分析各类型的puroindoline基因序列发现,Pinb-D1h和Pinb-D1i编码同一种氨基酸,突变后仍表现为软质是因为第28位精氨酸变成色氨酸,其余软质变异类型色氨酸丰富区附近均未发生变化,这可能是导致其胚乳质地仍未变硬的主要原因。结论在先前研究基础上,本试验对CIMMYT小麦基因型作了进一步分析,发现系谱中没有外源材料的所有CIMMYT硬质麦均为Pina-D1b类型。从山羊草引进的软质突变类型之间存在一定硬度差异。 | 陈锋 夏先春 Manila William Morten Lillemo Richard Trethowan Roberto J Peňa 何中虎 | 2006 | 中国农业科学2006，39，8： | 6 |
| 6 | 1423 Pancreaticoduodenectomies for Pancreatic Cancer: A Single-Institution Experience显示文摘 | Jordan M. Winter John L. Cameron Kurtis A. Campbell Meghan A. Arnold David C. Chang JoAnn Coleman Mary B. Hodgin Patricia K. Sauter Ralph H. Hruban Taylor S. Riall Richard D. Schulick Michael A. Choti Keith D. Lillemoe Charles J. Yeo | 2006 | Journal of Gastrointestinal Surgery2006，，9： | 5 |
| 7 | Resected adenocarcinoma of the pancreas— 616 patients: Results, outcomes, and prognostic indicators显示文摘 | Taylor A. Sohn M.D. Charles J. Yeo M.D. Jokn L. Cameron M.D. Leonidas Koniaris M.D. Sunjay Kaushal M.D. Ross A. Abrams M.D. Patricia K. Sauter A.C.N.P. JoAnn Coleman A.C.N.P. Ralph H. Hruban M.D. Keith D. Lillemoe M.D | 2000 | Journal of Gastrointestinal Surgery2000，，6： | 4 |
| 8 | Trends in Long-Term Survival Following Liver Resection for Hepatic Colorectal Metastases显示文摘 | Michael A. Choti James V. Sitzmann Marcelo F. Tiburi Wuthi Sumetchotimetha Ram Rangsin Richard D. Schulick Keith D. Lillemoe Charles J. Yeo John L. Cameron | 2002 | Annals of Surgery2002，，6： | 3 |
| 9 | Surgical Management of Bile Duct Injuries Sustained During Laparoscopic Cholecystectomy: Perioperative Results in 200 Patients显示文摘 | Jason K. Sicklick Melissa S. Camp Keith D. Lillemoe Genevieve B. Melton Charles J. Yeo Kurtis A. Campbell Mark A. Talamini Henry A. Pitt JoAnn Coleman Patricia A. Sauter John L. Cameron | 2005 | Annals of Surgery2005，，5： | 3 |
| 10 | Renal cell carcinoma metastatic to the pancreas: Results of surgical management显示文摘 | Taylor A. Sohn M.D. Charles J. Yeo M.D. John L. Cameron M.D. Attila Nakeeb M.D. Keith D. Lillemoe M.D | 2001 | Journal of Gastrointestinal Surgery2001，，4： | 3 |
| 11 | Prognostic relevance of lymph node ratio following pancreaticoduodenectomy for pancreatic cancer显示文摘 | Timothy M. Pawlik Ana L. Gleisner John L. Cameron Jordan M. Winter Lia Assumpcao Keith D. Lillemoe Christopher Wolfgang Ralph H. Hruban Richard D. Schulick Charles J. Yeo Michael A. Choti | 2007 | Surgery2007，，5： | 3 |
| 12 | Pancreatic Ductal Adenocarcinoma: Is There a Survival Difference for R1 Resections Versus Locally Advanced Unresectable Tumors? What Is a “True” R0 Resection?显示文摘 | Ioannis T. Konstantinidis Andrew L. Warshaw Jill N. Allen Lawrence S. Blaszkowsky Carlos Fernandez-del Castillo Vikram Deshpande Theodore S. Hong Eunice L. Kwak Gregory Y. Lauwers David P. Ryan Jennifer A. Wargo Keith D. Lillemoe Cristina R. Ferrone | 2013 | Annals of Surgery2013，，4： | 3 |
| 13 | Cholangiocarcinoma: A Spectrum of Intrahepatic, Perihilar, and Distal Tumors显示文摘 | Attila Nakeeb Henry A. Pitt Taylor A. Sohn JoAnn Coleman Ross A. Abrams Steven Piantadosi Ralph H. Hruban Keith D. Lillemoe Charles J. Yeo John L. Cameron | 1996 | Annals of Surgery1996，，4： | 3 |
| 14 | Risk factors and outcomes in postpancreaticoduodenectomy pancreaticocutaneous fistula显示文摘 | John W. Lin M.D. M. Eng. John L. Cameron M.D. Charles J. Yeo M.D. Taylor S. Riall M.D. Keith D. Lillemoe M.D | 2004 | Journal of Gastrointestinal Surgery2004，，8： | 2 |
| 15 | Global Wheat Head Detection 2021:An Improved Dataset for Benchmarking Wheat Head Detection Methods显示文摘The Global Wheat Head Detection(GWHD)dataset was created in 2020 and has assembled 193,634 labelled wheat heads from 4700 RGB images acquired from various acquisition platforms and 7 countries/institutions.With an associated competition hosted in Kaggle,GWHD_2020 has successfully attracted attention from both the computer vision and agricultural science communities.From this first experience,a few avenues for improvements have been identified regarding data size,head diversity,and label reliability.To address these issues,the 2020 dataset has been reexamined,relabeled,and complemented by adding 1722 images from 5 additional countries,allowing for 81,553 additional wheat heads.We now release in 2021 a new version of the Global Wheat Head Detection dataset,which is bigger,more diverse,and less noisy than the GWHD_2020 version. | Etienne David Mario Serouart Daniel Smith Simon Madec Kaaviya Velumani Shouyang Liu Xu Wang Francisco Pinto Shahameh Shafiee Izzat SATahir Hisashi Tsujimoto Shuhei Nasuda Bangyou Zheng Norbert Kirchgessner Helge Aasen Andreas Hund Pouria Sadhegi-Tehran Koichi Nagasawa Goro Ishikawa Sébastien Dandrifosse Alexis Carlier Benjamin Dumont Benoit Mercatoris Byron Evers Ken Kuroki Haozhou Wang Masanori Ishii Minhajul ABadhon Curtis Pozniak David Shaner LeBauer Morten Lillemo Jesse Poland Scott Chapman Benoit de Solan Frédéric Baret Ian Stavness Wei Guo | 2021 | Plant Phenomics2021，3，1： | 2 |
| 16 | A Lethally Irradiated Allogeneic Granulocyte-Macrophage Colony Stimulating Factor-Secreting Tumor Vaccine for Pancreatic Adenocarcinoma: A Phase II Trial of Safety, Efficacy, and Immune Activation显示文摘 | Lutz Eric Charles J. Yeo Keith D. Lillemoe Barbara Biedrzycki Barry Kobrin Joseph Herman Elizabeth Sugar Steven Piantadosi John L. Cameron Sara Solt Beth Onners Irena Tartakovsky Miri Choi Rajni Sharma Peter B. Illei Hruban Ralph H. Ross A. Abrams Dung Le | 2011 | Annals of Surgery2011，，2： | 2 |
| 17 | Panceraicoduodenetomy for cancer of the head of the pancreas: 201 patients 显示文摘 | YEO CJ CAMERON JL LILLEMOE KD | 1995 | Ann Surg1995，221，6： | 2 |
| 18 | Trends in Long-Term Survival Following Liver Resection for Hepatic Colorectal Metastases显示文摘 | Michael A. Choti James V. Sitzmann Marcelo F. Tiburi Wuthi Sumetchotimetha Ram Rangsin Richard D. Schulick Keith D. Lillemoe Charles J. Yeo John L. Cameron | 2002 | Annals of Surgery2002，，6： | 2 |
| 19 | A Margin-Negative R0 Resection Accomplished With Minimal Postoperative Complications Is the Surgeon’s Contribution to Long-Term Survival in Pancreatic Cancer显示文摘 | Thomas J. Howard Joseph E. Krug Jian Yu Nick J. Zyromski C. Max Schmidt Lewis E. Jacobson James A. Madura Eric A. Wiebke Keith D. Lillemoe | 2006 | Journal of Gastrointestinal Surgery2006，，10： | 2 |
| 20 | Survival after gastric adenocarcinoma resection: Eighteen-year experience at a single institution显示文摘 | Steven C. Cunningham M.D. Farin Kamangar M.D. M.P.H. Min P. Kim M.D. Sommer Hammoud Raqeeb Haque Anirban Maitra M.B.B.S. Elizabeth Montgomery M.D. Richard E. Heitmiller M.D. F.A.C.S. Michael A. Choti M.D. F.A.C.S. Keith D. Lillemoe M.D. F.A.C.S. John | 2005 | Journal of Gastrointestinal Surgery2005，，5： | 2 |
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