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| 1 | A rapid advice guideline for the diagnosis and treatment of 2019 novel coronavirus(2019-nCoV) infected pneumonia(standard version)显示文摘In December 2019, a new type viral pneumonia cases occurred in Wuhan, Hubei Province;and then named '2019 novel coronavirus(2019-nCoV)' by the World Health Organization(WHO) on 12 January 2020. For it is a never been experienced respiratory disease before and with infection ability widely and quickly, it attracted the world’s attention but without treatment and control manual. For the request from frontline clinicians and public health professionals of 2019-nCoV infected pneumonia management, an evidence-based guideline urgently needs to be developed. Therefore, we drafted this guideline according to the rapid advice guidelines methodology and general rules of WHO guideline development;we also added the first-hand management data of Zhongnan Hospital of Wuhan University. This guideline includes the guideline methodology, epidemiological characteristics, disease screening and population prevention, diagnosis, treatment and control(including traditional Chinese Medicine), nosocomial infection prevention and control, and disease nursing of the 2019-nCoV. Moreover, we also provide a whole process of a successful treatment case of the severe 2019-nCoV infected pneumonia and experience and lessons of hospital rescue for 2019-nCoV infections. This rapid advice guideline is suitable for the first frontline doctors and nurses, managers of hospitals and healthcare sections, community residents, public health persons, relevant researchers, and all person who are interested in the 2019-nCoV. | Ying-Hui Jin Lin Cai Zhen-Shun Cheng Hong Cheng Tong Deng Yi-Pin Fan Cheng Fang Di Huang Lu-Qi Huang Qiao Huang Yong Han Bo Hu Fen Hu Bing-Hui Li Yi-Rong Li Ke Liang Li-Kai Lin Li-Sha Luo Jing Ma Lin-Lu Ma Zhi-Yong Peng Yun-Bao Pan Zhen-Yu Pan Xue-Qun Ren Hui-Min Sun Ying Wang Yun-Yun Wang Hong Weng Chao-Jie Wei Dong-Fang Wu Jian Xia Yong Xiong Hai-Bo Xu Xiao-Mei Yao Yu-Feng Yuan Tai-Sheng Ye Xiao-Chun Zhang Ying-Wen Zhang Yin-Gao Zhang Hua-Min Zhang Yan Zhao Ming-Juan Zhao Hao Zi Xian-Tao Zeng Yong-Yan Wang Xing-Huan Wang 无 | 2020 | Military Medical Research2020，7，1： | 161 |
| 2 | Effectiveness of Different Waist Circumference Cut-off Values in Predicting Metabolic Syndrome Prevalence and Risk Factors in Adults in China显示文摘Objective To study the effectiveness of waist circumference cut-off values in predicting the prevalence of metabolic syndrome(MetS) and risk factors in adults in China. Methods A cross-sectional survey was condcuted in 14 provinces(autonomous region, municipality) in China. A total of 47 325 adults aged ≥20 years were selected by multistage stratified sampling, and questionnaire survey and physical and clinical examination were conducted among them. MetS was defined according to the International Diabetes Federation(IDF) criteria and modified IDF criteria.Results The age-standardized prevalence of MetS was 24.2%(22.1% in men and 25.8% in women) and 19.5%(22.1% in men and 18.0% in women) according to the IDF criteria and modified IDF criteria respectively. The age-standardized prevalence of pre-MetS was 8.1%(8.6% in men and 7.8% in women) according to the modified IDF criteria. The prevalence of MetS was higher in urban residents than rural residents and in northern China residents than in southern China residents. The prevalence of central obesity was about 30% in both men and women according to the ethnicity-specific cut-off values of waist circumference for central obesity(90 cm for men and 85 cm for women). Multivariate regression analysis revealed no significant difference in risk factors between the two MetS definitions. Conclusion Using both the modified IDF criteria and ethnicity-specific cut-off values of waist circumference can provide more useful information about the prevalence of MetS in China. | ZHOU Hai Cheng LAI Ya Xin SHAN Zhong Yan JIA Wei Ping YANG Wen Ying LU Ju Ming WENG Jian Ping JI Li Nong LIU Jie TIAN Hao Ming JI Qiu He ZHU Da Long CHEN Li GUO Xiao Hui ZHAO Zhi Gang Li Qiang ZHOU Zhi Guang GE Jia Pu SHAN Guang Liang | 2014 | Biomedical and Environmental Sciences2014，27，5： | 17 |
| 3 | Two-dimensional nanomaterials: fascinating materials in biomedical field显示文摘Due to their high anisotropy and chemical functions,two-dimensional(2D)nanomaterials have attracted increasing interest and attention from various scientific fields,including functional electronics,catalysis,supercapacitors,batteries and energy materials.In the biomedical field,2D nanomaterials have made significant contributions to the field of nanomedicine,especially in drug/gene delivery systems,multimodal imaging,biosensing,antimicrobial agents and tissue engineering.2D nanomaterials such as graphene/graphene oxide(GO)/reduced graphene oxide(r GO),silicate clays,layered double hydroxides(LDHs),transition metal dichalcogenides(TMDs),transition metal oxides(TMOs),black phosphorus(BP),graphitic carbon nitride(g-C3N4),hexagonal boron nitride(h-BN),antimonene(AM),boron nanosheets(B NSs)and tin telluride nanosheets(Sn Te NSs)possess excellent physical,chemical,optical and biological properties due to their uniform shapes,high surface-to-volume ratios and surface charge.In this review,we first introduce the properties,structures and synthetic strategies of different configurations of 2D nanomaterials.Recent advances and paradigms of 2D nanomaterials in a variety of biomedical applications,ranging from drug delivery,cancer treatment,bioimaging and tissue engineering to biosensing are discussed afterwards.In the final part,we foresee the development prospects and challenges of 2D nanomaterials after summarizing the research status of ultrathin 2D nanomaterials. | Tingting Hu Xuan Mei Yingjie Wang Xisheng Weng Ruizheng Liang Min Wei | 2019 | Science Bulletin2019，64，22： | 12 |
| 4 | Resistant mutants induced by adefovir dipivoxil in hepatitis B virus isolates显示文摘AIM:To investigate the loci of adefovir dipivoxil(ADV)-induced resistance in hepatitis B virus(HBV)isolates and optimize the management of ADV-treated patients.METHODS:Between June 2008 and August 2010,a cross-sectional control study was conducted comprising 79 patients with chronic HBV infection-related liver disease who had been administered ADV monotherapy.Patients underwent liver imaging.Serum DNA extracts were analyzed for HBV DNA levels,genotypes,and serology markers,and deep sequencing of the HBV P gene was performed.RESULTS:ADV-resistant patients were found either with a single mutated locus,or with coexisting mutated loci.The most prevalent mutations were rt A181T,rt V214A,and rt N236T.Twenty-six patients had more than two mutated loci.The mutants were distributed among the patients without any significant affinity for gender,age,end-stage of liver disease,complications of non-alcoholic fatty liver disease,or HBV DNA levels.Patients with the rt A181T mutant were primarily infected with genotype C and e-antigen negative HBV,while patients with the rt N236T mutant were primarily infected by genotype B HBV(χ2=6.004,7.159;P=0.023,0.007).The duration of treatment with ADV was shorter in the single mutant group compared with the multi-mutant group(t=2.426,P=0.018).CONCLUSION:Drug-resistant HBV mutants are complex and diverse.Patients should receive the standard and first-line antiviral treatment,strictly comply with medication dosage,and avoid short-term withdrawal. | Su-Wen Jiang Li-Peng Yao Ai-Rong Hu Yao-Ren Hu Shi-Xiang Chen Tao Xiong Guo-Sheng Gao Xiao-Yue Liang Shi-Xiong Ding Peng-Jian Weng | 2014 | World Journal of Gastroenterology2014，20，45： | 12 |
| 5 | A Simplified PCR-SSP Method for HLA-A2 Subtype in a Population of Wuhan,China显示文摘HLA-A2 is the most frequent HLA-A allele in all ethnic populations, and an important restriction element for peptide presentation to T cells in infectious disease and cancer. However, the HLA-A2 supertype consisting of up to 75 subtypes, mutation studies and analyses using cytotoxic T lymphocytes suggest the functional relevance of subtype-specific differences in HLA-A2 molecules for peptide binding and T-cell recognition. Therefore, it is necessary for T-cell response study to discriminate the HLA-A2 subtypes and to understand the profile of HLA-A2 allelic distribution in a given population. In this study, we developed a simple, robust approach based on the nested polymerase chain reaction using sequence-specific primers (PCR-SSP) to discriminate 17 HLA-A2 subtypes which cover the most HLA-A2 alleles (> 99% allele frequency) reported in Chinese, using 15 combinations of 19 allelic specific primers. In the first round of PCR, 3 combinations of 5 primers were used to determine whether the tested sample was HLA-A2 positive, meanwhile the subtypes of HLA-A*0209 and HLA-A*0215N were determined for the variant position of these two subtypes is in exon 4 instead of exon 2, 3. Samples of HLA-A2 positive were subtyped in the second round of PCR, using PCR products of the first round as templates. This strategy was applied to test the samples of 78 random HLA-A2 positive individuals for their HLA-A2 subtypes. Those samples were screened for HLA-A2 positive by the first round PCR-SSP from 154 healthy blood donors in Wuhan, China. The subtyping results were verified by using flow cytometric analysis (FCM) with HLA-A2 specific monoclonal antibody BB7.2 and DNA sequencing. The typing results of the samples show 50.7% random individuals in the population carry HLA-A2, HLA-A*0201 ranks the first (allele frequency = 15.5%), followed by A*0207 (5.8%), A*0206 (4.7%), A*0203 (2.6%), A*0210 (0.7%), and these 5 alleles account for 99.0% HLA-A2 subtypes of allele frequency. Our study indicates that the developed typing method is simple and reliable for HLA-A2 subtyping in Chinese, and the profile of allelic distribution of HLA-A2 subtypes is revealed in the population of Wuhan, China. | Bing Liang Lijun Zhu Zhihui Liang Xiufang Weng Xiaoling Lu Cai'e Zhang Hui Li Xiongwen Wu | 2006 | Cellular & Molecular Immunology2006，3，6： | 9 |
| 6 | Comparison of the analgesic effects between electro-acupuncture and moxibustion with visceral hypersensitivity rats in irritable bowel syndrome显示文摘AIM To observe whether there are differences in the effects of electro-acupuncture(EA) and moxibustion(Mox) in rats with visceral hypersensitivity. METHODS EA at 1 m A and 3 m A and Mox at 43?℃ and 46?℃ were applied to the Shangjuxu(ST37, bilateral) acupoints in model rats with visceral hypersensitivity. Responses of wide dynamic range neurons in dorsal horns of the spinal cord were observed through the extracellular recordings. Mast cells(MC) activity in the colons of rats were assessed, and 5-hydroxytryptamine(5-HT), 5-hydroxytryptamine 3 receptor(5-HT3R) and 5-HT4Rexpressions in the colons were measured.RESULTS Compared with normal control group, responses of wide dynamic range neurons in the dorsal horn of the spinal cord were increased in the EA at 1 m A and 3 m A groups(1 m A: 0.84 ± 0.74 vs 2.73 ± 0.65, P < 0.001; 3 m A: 1.91 ± 1.48 vs 6.44 ± 1.26, P < 0.001) and Mox at 43?℃ and 46?℃ groups(43?℃: 1.76 ± 0.81 vs 4.14 ± 1.83, P = 0.001; 46?℃: 5.19 ± 2.03 vs 7.91 ± 2.27, P = 0.01). MC degranulation rates and the expression of 5-HT, 5-HT3 R and 5-HT4 R in the colon of Mox 46?℃ group were decreased compared with model group(MC degranulation rates: 0.47 ± 0.56 vs 0.28 ± 0.78, P < 0.001; 5-HT: 1.42 ± 0.65 vs 7.38 ± 1.12, P < 0.001; 5-HT3R: 6.62 ± 0.77 vs 2.86 ± 0.88, P < 0.001; 5-HT4R: 4.62 ± 0.65 vs 2.22 ± 0.97, P < 0.001). CONCLUSION The analgesic effects of Mox at 46?℃ are greater than those of Mox at 43?℃, EA 1 m A and EA 3 m A. | Ji-Meng Zhao Liang Li Liu Chen Yin Shi Yu-Wei Li Hai-Xia Shang Lu-Yi Wu Zhi-Jun Weng Chun-Hui Bao Huan-Gan Wu | 2017 | World Journal of Gastroenterology2017，23，16： | 9 |
| 7 | Shale Gas Formation and Occurrence in China: An Overview of the Current Status and Future Potential显示文摘Shale gas is one of the most promising unconventional resources both in China and abroad. It is known as a form of self-contained source-reservoir system with large and continuous dimensions. Through years of considerable exploration efforts, China has identified three large shale gas fields in the Fuling, Changning and Weiyuan areas of the Sichuan Basin, and has announced more than 540 billion m^3 of proven shale gas reserves in marine shale systems. The geological theories for shale gas development have progressed rapidly in China as well. For example, the new depositional patterns have been introduced for deciphering the paleogeography and sedimentary systems of the Wufeng shale and Longmaxi shale in the Sichuan Basin. The shale gas storage mechanism has been widely accepted as differing from conventional natural gas in that it is adsorbed on organic matter or a mineral surface or occurs as free gas trapped in pores and fractures of the shale. Significant advances in the techniques of microstructural characterization have provided new insights on how gas molecules are stored in micro- and nano-scale porous shales. Furthermore, newly-developed concepts and practices in the petroleum industry, such as hydraulic fracturing, microseismic monitoring and multiwell horizontal drilling, have made the production of this unevenly distributed but promising unconventional natural gas a reality. China has 10–36 trillion m^3 of promising shale gas among the world's whole predicted technically recoverable reserves of 206.6 trillion m^3. China is on the way to achieving its goal of an annual yield of 30–50 billion m^3 by launching more trials within shale gas projects. | ZOU Caineng YANG Zhi PAN Songqi CHEN Yanyan LIN Senhu HUANG Jinliang WU Songtao DONG Dazhong WANG Shufang LIANG Feng SUN Shasha HUANG Yong WENG Dingwei | 2016 | Acta Geologica Sinica(English Edition)2016，90，4： | 9 |
| 8 | Electroacupuncture at He-Mu points reduces P2X_4 receptor expression in visceral hypersensitivity显示文摘Electroacupuncture at Shangjuxu (ST37) and Tianshu (ST25) was reported to improve visceral hypersensitivity in rats.Colorectal distension was utilized to generate a rat model of chronic visceral hypersensitivity in irritable bowel syndrome.Results showed that abdominal withdrawal reflex scores noticeably increased after model establishment.Simultaneously,P2X 4 receptor immunoreactivity significantly increased in the colon and spinal cord.Electroacupuncture and pinaverium bromide therapy both markedly decreased abdominal withdrawal reflex scores in rats with visceral hypersensitivity,and significantly decreased P2X 4 receptor immunoreactivity in the colon and spinal cord.These data suggest that electroacupuncture treatment can improve visceral hypersensitivity in rats with irritable bowel syndrome by diminishing P2X 4 receptor immunoreactivity in the colon and spinal cord. | Xinxin Guo Jifei Chen Yuan Lu Luyi Wu Zhijun Weng Ling Yang Yuhu Xin Xianming Lin Yi Liang Jianqiao Fang | 2013 | Neural Regeneration Research2013，8，22： | 9 |
| 9 | OsCIPK7 point-mutation leads to conformation and kinase-activity change for sensing cold response显示文摘Calcineurin B-like interacting protein kinases(CIPKs) play important roles via environmental stress.However, less is known how to sense the stress in molecular structure conformation level. Here, an Os CIPK7 mutant via TILLING procedure with a point mutation in the kinase domain showed increased chilling tolerance, which could be potentially used in the molecular breeding. We found that this point mutation of Os CIPK7 led to a conformationalchange in the activation loop of the kinase domain,subsequently with an increase of protein kinase activity,thus conferred an increased tolerance to chilling stress. | Dajian Zhang Xiaoyu Guo Yunyuan Xu Hao Li Liang Ma Xuefeng Yao Yuxiang Weng Yan Guo Chun-Ming Liu Kang Chong | 2019 | Journal of Integrative Plant Biology2019，61，12： | 8 |
| 10 | Anterior vs conventional approach right hepatic resection for large hepatocellular carcinoma:A systematic review and meta-analysis显示文摘AIM To compare the clinical outcomes of right hepatectomy for large hepatocellular carcinoma via the anterior and conventional approach.METHODS We comprehensively performed an electronic search of Pub Med, EMBASE, and the Cochrane Library for randomized controlled trials(RCTs) or controlled clinical trials(CCTs) published between January 2000 and May 2017 concerning the anterior approach(AA) and the conventional approach(CA) to right hepatectomy. Studies that met the inclusion criteria were included, and their outcome analyses were further assessed using a fixed or random effects model.RESULTS This analysis included 2297 patients enrolled in 16 studies(3 RCTs and 13 CTTs). Intraoperative blood loss [weighted mean difference =-255.21; 95% confidence interval(95%CI):-371.3 to-139.12; P < 0.0001], intraoperative blood transfusion [odds ratio(OR) = 0.42; 95%CI: 0.29-0.61; P < 0.0001], mortality(OR = 0.59; 95%CI: 0.38-0.92; P = 0.02), morbidity(OR = 0.77; 95%CI: 0.62-0.95; P = 0.01), and recurrencerate(OR = 0.62; 95%CI: 0.47-0.83; P = 0.001) were significantly reduced in the AA group. Patients in the AA group had better overall survival(hazard ratio [HR] = 0.71; 95%CI: 0.50-1.00; P = 0.05) and disease-free survival(HR = 0.67; 95%CI: 0.58-0.79; P < 0.0001) than those in the CA group.CONCLUSION The AA is safe and effective for right hepatectomy for large hepatocellular carcinoma and could accelerate postoperative recovery and achieve better survival outcomes than the CA. | Jian-Xin Tang Jin-Jun Li Rui-Hui Weng Zi-Ming Liang Nan Jiang | 2017 | World Journal of Gastroenterology2017，23，44： | 7 |
| 11 | Efficient hepatic delivery and protein expression enabled by optimized mRNA and ionizable lipid nanoparticle显示文摘mRNA is a novel class of therapeutic modality that holds great promise in vaccination,protein replacement therapy,cancer immunotherapy,immune cell engineering etc.However,optimization of mRNA molecules and efficient in vivo delivery are quite important but challenging for its broad application.Here we present an ionizable lipid nanoparticle(iLNP)based on iBL0713 lipid for in vitro and in vivo expression of desired proteins using codon-optimized mRNAs.mRNAs encoding luciferase or erythropoietin(EPO)were prepared by in vitro transcription and formulated with proposed iLNP,to form iLP171/mRNA formulations.It was revealed that both luciferase and EPO proteins were successfully expressed by human hepatocellular carcinoma cells and hepatocytes.The maximum amount of protein expression was found at 6 h post-administration.The expression efficiency of EPO with codon-optimized mRNA was significantly higher than that of unoptimized mRNA.Moreover,no toxicity or immunogenicity was observed for these mRNA formulations.Therefore,our study provides a useful and promising platform for mRNA therapeutic development. | Tongren Yang Chunhui Li Xiaoxia Wang Deyao Zhao Mengjie Zhang Huiqing Cao Zicai Liang Haihua Xiao Xing-Jie Liang Yuhua Weng Yuanyu Huang | 2020 | Bioactive Materials2020，5，4： | 7 |
| 12 | Macrophage-NLRP3 Inflammasome Activation Exacerbates Cardiac Dysfunction after Ischemic Stroke in a Mouse Model of Diabetes显示文摘Ischemic stroke is one of the leading causes of death worldwide.In the post-stroke stage,cardiac dysfunction is common and is known as the brain-heart interaction.Diabetes mellitus worsens the post-stroke outcome.Stroke-induced systemic inflammation is the major causative factor for the sequential complications,but the mechanism underlying the brain-heart interaction in diabetes has not been clarified.The NLRP3(NLR pyrin domain-containing 3) inflammasome,an important component of the inflammation after stroke,is mainly activated in M1-polarized macrophages.In this study,we found that the cardiac dysfunction induced by ischemic stroke is more severe in a mouse model of type 2 diabetes.Meanwhile,M1-polarized macrophage infiltration and NLRP3 inflammasome activation increased in the cardiac ventricle after diabetic stroke.Importantly,the NLRP3 inflammasome inhibitor CY-09 restored cardiac function,indicating that the Ml-polarized macrophage-NLRP3 inflammasome activation is a pathway underlying the brain-heart interaction after diabetic stroke. | Hong-Bin Lin Guan-Shan Wei Feng-Xian Li Wen-Jing Guo Pu Hong Ya-Qian Weng Qian-Qian Zhang Shi-Yuan Xu Wen-Bin Liang Zhi-Jian You Hong-Fei Zhang | 2020 | Neuroscience Bulletin2020，36，9： | 7 |
| 13 | Lung-protective Ventilation in Patients with Brain Injury: A Multicenter Cross-sectional Study and Questionnaire Survey in China显示文摘 | Xu-Ying Luo Ying-Hong Hu Xiang-Yuan Cao Yan Kang Li-Ping Liu Shou-Hong Wang Rong-Guo Yu Xiang-You Yu Xia Zhang Bao-Shan Li Zeng-Xiang Ma Yi-Bing Weng Heng Zhang De-Chang Chen Wei Chen Wen-Jin Chen Xiu-Mei Chen Bin Du Mei-Li Duan Jin Hu Yun-Feng Hoang Gui-Jun Jia Li-Hong Li Yu-Min Liang Bing-Yu Qin Xian-Dong Wang Jian Xiong Li-Mei Yan Zheng-Ping Yang Chen-Ming Dong Dong-Xin Wang Qing-Yuan Zhan Shuang-Lin FU Lin Zhao Qi-Bing Huang Ying-Guang Xie Xiao-Bo Huang Guo-Bin Zhang Wang-Bin Xu Yuan Xu YaLing Liu He-Ling Zhao Rong-Qing Sun Ming Sun Qing-Hong Cheng Xin Qu Xiao-Feng Yang Ming Xu Zhong-Hua Shi Han Chen Xuan He Yan-Lin Yang Guang-Qiang Chen Xiu-Mei Sun Jian-Xin Zhou | 2016 | Chinese Medical Journal2016，，14： | 6 |
| 14 | Nanotechnology-based strategies for treatment of ocular disease显示文摘Ocular diseases include various anterior and posterior segment diseases. Due to the unique anatomy and physiology of the eye, efficient ocular drug delivery is a great challenge to researchers and pharmacologists. Although there are conventional noninvasive and invasive treatments, such as eye drops,injections and implants, the current treatments either suffer from low bioavailability or severe adverse ocular effects. Alternatively, the emerging nanoscience and nanotechnology are playing an important role in the development of novel strategies for ocular disease therapy. Various active molecules have been designed to associate with nanocarriers to overcome ocular barriers and intimately interact with specific ocular tissues. In this review, we highlight the recent attempts of nanotechnology-based systems for imaging and treating ocular diseases, such as corneal d iseases, glaucoma, retina diseases, and choroid diseases. Although additional work remains, the progress described herein may pave the way to new,highly effective and important ocular nanomedicines. | Yuhua Weng Juan Liu Shubin Jin Weisheng Guo Xingjie Liang Zhongbo Hu | 2017 | Acta Pharmaceutica Sinica B2017，7，3： | 6 |
| 15 | Identification of Functional Genetic Variations Underlying Drought Tolerance in Maize Using SNP Markers显示文摘Single nucleotide polymorphism (SNP) is a common form of genetic variation and popularly exists in maize genome. An Illumina GoldenGate assay with 1 536 SNP markers was used to genotype maize inbred lines and identified the functional genetic variations underlying drought tolerance by association analysis. Across 80 lines, 1 006 polymorphic SNPs (65.5% of the total) in the assay with good call quality were used to estimate the pattern of genetic diversity, population structure, and familial relatedness. The analysis showed the best number of fixed subgroups was six, which was consistent with their original sources and results using only simple sequence repeat markers. Pairwise linkage disequilibrium (LD) and association mapping with phenotypic traits investigated under water-stressed and well-watered regimes showed rapid LD decline within 100-500 kb along the physical distance of each chromosome, and that 29 SNPs were associated with at least two phenotypic traits in one or more environments, which were related to drought-tolerant or drought-responsive genes. These drought-tolerant SNPs could be converted into functional markers and then used for maize improvement by marker-assisted selection. | Zhuanfang Hao Xinhai Li Chuanxiao Xie Jianfeng Weng Mingshun Li Degui Zhang Xiaoling Liang Lingling Liu Sisi Liu Shihuang Zhang | 2011 | Journal of Integrative Plant Biology2011，53，8： | 5 |
| 16 | Over-Expression of Bn MAPK1 in Brassica napus Enhances Tolerance to Drought Stress显示文摘Mitogen-activated protein kinases(MAPKs) are a family of Ser/Thr protein kinases widely conserved in all eukaryotes and involved in responses to biotic and abiotic stresses. In this study, two over-expressing Bn MAPK1 oilseed rape lines, ov3 and ov11, were used to study the drought-resistant mechanism of Bn MAPK1 under natural drought and simulation drought through spraying 10% PEG 8000 in seedlings. Zhongyou 821(WT) was used as control. Compared with wild type, transgenic seedlings had higher leaf water content, higher root activity, slightly higher peroxidase(POD) and superoxide dismutase(SOD) activity, higher proline content and lower malondialdehyde(MDA) content. The expression of drought-resistant related genes, including P5 CSB, PLC, LEA4 and SCE1, have been up-regulated in some degree and the expressed time of transgenic lines were earlier than that of wild type. These results suggested that over-expression of Bn MAPK1 can enhance the resistance to drought in oilseed rape(Brassica napus). | WENG Chang-mei LU Jun-xing WAN Hua-fang WANG Shu-wen WANG Zhen LU Kun LIANG Ying | 2014 | Journal of Integrative Agriculture2014，13，11： | 5 |
| 17 | Numerical investigation on the effect of depletion-induced stress reorientation on infill well hydraulic fracture propagation显示文摘Depletion-induced stress change causes the redistribution of stress field in reservoirs,which can lead to the reorientation of principal stresses.Stress reorientation has a direct impact on fracture propagation of infill wells.To understand the effect of stress reorientation on the propagation of infill well’s fractures,an integrated simulation workflow that combines the reservoir flow calculation and the infill well hydraulic fracturing modeling is adopted.The reservoir simulation is computed to examine the relationship between the extent of stress reversal region and reservoir properties.Then,the hydraulic fracturing model considering the altered stress field for production is built to characterize the stress evolution of secondary fracturing.Numerical simulations show that stress reorientation may occur due to the decreasing of the horizontal stresses in an elliptical region around the parent well.Also,the initial stress difference is the driving factor for stress reorientation.However,the bottom hole pressure,permeability and other properties connected with fluid flow control timing of the stress reorientation.The decrease of the horizontal stresses around the parent well lead to asymmetrical propagation of a hydraulic fracture of the infill well.The study provides insights on understanding the influence of stress reorientation to the infill well fracturing treatment and interference between parent and infill wells. | Feng-Shou Zhang Liu-Ke Huang Lin Yang Egor Dontsov Ding-Wei Weng Hong-Bo Liang Zi-Rui Yin Ji-Zhou Tang | 2022 | Petroleum Science2022，19，1： | 4 |
| 18 | Seismic cyclic loading test of SRC columns confined with 5-spirals显示文摘Presented herein is an experimental study on seismic resistance of rectangular steel reinforced concrete (SRC) columns confined with a new type of multi-spiral cage. The multi-spiral cage is a device of five interconnected spirals, named '5-spirals', with a large spiral at the center and four small ones at the corners. The innovation of applying the 5-spirals to SRC column is to take its superiority in concrete confinement and efficiency in automatic production for the precast construction industry. Four full-scale SRC columns were tested under horizontal cyclic loading. All of the tested columns were capable of sustaining a drift angle up to 6% radians. The hysteresis loops observed from the cyclic loading tests indicated that the spirally confined SRC columns demonstrated excellent performances in both strength and ductility. The test results suggested that with significant saving of the confinement steel, the newly innovated 5-spirals can be successfully applied to the precast rectangular SRC columns. | WENG ChengChiang YIN YenLiang WANG JuiChen LIANG ChingYu | 2008 | Science China(Technological Sciences)2008，51，5： | 4 |
| 19 | Therapeutic siRNA:state of the art显示文摘RNA interference(RNAi)is an ancient biological mechanism used to defend against external invasion.It theoretically can silence any disease-related genes in a sequence-specific manner,making small interfering RNA(siRNA)a promising therapeutic modality.After a two-decade journey from its discovery,two approvals of siRNA therapeutics,ONPATTRO®(patisiran)and GIVLAARI™(givosiran),have been achieved by Alnylam Pharmaceuticals.Reviewing the long-term pharmaceutical history of human beings,siRNA therapy currently has set up an extraordinary milestone,as it has already changed and will continue to change the treatment and management of human diseases.It can be administered quarterly,even twice-yearly,to achieve therapeutic effects,which is not the case for small molecules and antibodies.The drug development process was extremely hard,aiming to surmount complex obstacles,such as how to efficiently and safely deliver siRNAs to desired tissues and cells and how to enhance the performance of siRNAs with respect to their activity,stability,specificity and potential off-target effects.In this review,the evolution of siRNA chemical modifications and their biomedical performance are comprehensively reviewed.All clinically explored and commercialized siRNA delivery platforms,including the GalNAc(N-acetylgalactosamine)–siRNA conjugate,and their fundamental design principles are thoroughly discussed.The latest progress in siRNA therapeutic development is also summarized.This review provides a comprehensive view and roadmap for general readers working in the field. | Bo Hu Liping Zhong Yuhua Weng Ling Peng Yuanyu Huang Yongxiang Zhao Xing-Jie Liang | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 4 |
| 20 | Insulin therapy stimulates lipid synthesis and improves endocrine functions of adipocytes in dietary obese C57BL/6 mice显示文摘 | Xiang CHEN Qiu-qiong YU Yan-hua ZHU Yan BI Wei-ping SUN Hua LIANG Meng-ying CAI Xiao-ying HE Jian-ping WENG | 2010 | Acta Pharmacologica Sinica2010，31，3： | 4 |
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