|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Diagnosis of gluten related disorders: Celiac disease, wheat allergy and non-celiac gluten sensitivity显示文摘Cereal crops and cereal consumption have had a vital role in Mankind's history. In the recent years gluten ingestion has been linked with a range of clinical disorders. Gluten-related disorders have gradually emerged as an epidemiologically relevant phenomenon with an estimated global prevalence around 5%. Celiac disease, wheat allergy and non-celiac gluten sensitivity represent different gluten-related disorders. Similar clinical manifestations can be observed in these disorders, yet there are peculiar pathogenetic pathways involved in their development. Celiac disease and wheat allergy have been extensively studied, while non-celiac gluten sensitivity is a relatively novel clinical entity, believed to be closely related to other gastrointestinal functional syndromes. The diagnosis of celiac disease and wheat allergy is based on a combination of findings from the patient's clinical history and specific tests, including serology and duodenal biopsies in case of celiac disease, or laboratory and functional assays for wheat allergy. On the other hand, non-celiac gluten sensitivity is still mainly a diagnosis of exclusion, in the absence of clear-cut diagnostic criteria. A multimodal pragmatic approach combining findings from the clinical history, symptoms, serological and histological tests is required in order to reach an accurate diagnosis. A thorough knowledge of the differences and overlap in clinical presentation among gluten-related disorders, and between them and other gastrointestinal disorders, will help clinicians in the process of differential diagnosis. | Luca Elli Federica Branchi Carolina Tomba Danilo Villalta Lorenzo Norsa Francesca Ferretti Leda Roncoroni Maria Teresa Bardella | 2015 | World Journal of Gastroenterology2015，21，23： | 8 |
| 2 | Cytoskeleton reorganization and ultrastructural damage induced by gliadin in a three-dimensional in vitro model显示文摘AIM: To evaluate the interplay between gliadin and LoVo cells and the direct effect of gliadin on cytoskeletal patterns.METHODS: We treated LoVo multicellular spheroids with digested bread wheat gliadin in order to investigate their morphology and ultrastructure (by means of light microscopy and scanning electron microscopy), and the effect of gliadin on actin (phalloidin fluorescence)and the tight-junction protein occludin and zonula occluden-1.RESULTS: The treated spheroids had deep holes and surface blebs, whereas the controls were smoothly surfaced ovoids. The incubation of LoVo spheroids with gliadin decreased the number of intracellular actin filaments, impaired and disassembled the integrity of the tight-junction system.CONCLUSION: Our data obtained from an 'in vivolike' polarized culture system confirm the direct noxious effect of gliadin on the cytoskeleton and tight junctions of epithelial cells. Unlike two-dimensional cell culture systems, the use of multicellular spheroids seems to provide a suitable model for studying cell-cell interactions. | Ersilia Dolfini Leda Roncoroni Luca Elli Chiara Fumagalli Roberto Colombo Simona Ramponi Fabio Forlani Maria Teresa Bardella | 2005 | World Journal of Gastroenterology2005，11，48： | 3 |
| 3 | Non-celiac gluten sensitivity:Time for sifting the grain显示文摘In the last few years, a new nomenclature has been proposed for the disease induced by the ingestion of gluten, a protein present in wheat, rice, barley and oats. Besides celiac disease and wheat allergy, the most studied forms of gluten-related disorders characterized by an evident immune mechanism(autoimmune in celiac disease and Ig E-mediated in wheat allergy), a new entity has been included, apparently not driven by an aberrant immune response: the non-celiac gluten sensitivity(NCGS). NCGS is characterized by a heterogeneous clinical picture with intestinal and extraintestinal symptoms arising after gluten ingestion and rapidly improving after its withdrawal from the diet. The pathogenesis of NCGS is largely unknown, but a mixture of factors such as the stimulation of the innate immune system, the direct cytotoxic effects of gluten, and probably the synergy with other wheat molecules, are clues for the complicated puzzle. In addition, the diagnostic procedures still remain problematic due to the absence of efficient diagnostic markers; thus, diagnosis is based upon the symptomatic response to a gluten-free diet and the recurrence of symptoms after gluten reintroduction with the possibility of an important involvement of a placebo effect. The temporary withdrawal of gluten seems a reasonable therapy, but the timing of gluten reintroduction and the correct patient management approach are have not yet been determined. | Luca Elli Leda Roncoroni Maria Teresa Bardella | 2015 | World Journal of Gastroenterology2015，21，27： | 1 |
| 4 | Damaging effects of gliadin on three-dimensional cell culture model显示文摘AIM: To evaluate the effects of gliadin on the oxidative environment in the'in vivo-like' model of a three-dimensional cell culture system.METHODS: LoVo cell line (intestinal adenocarcinoma)multicellular spheroids were treated with digested gliadin (with albumin used as a control). Spheroid volumes, cell viability and morphology, lactate dehydrogenase (LDH)release, content of reduced glutathione (GSH) and activity of GSH-related enzymes were examined. The data were statistically analyzed using the Student's t-test (P<0.05).was considered statistically significant.RESULTS: Gliadin reduced cell viability (from 20% to 60%)and led to morphological alterations characterized by apoptotic findings and cytoskeletal injuries. LDH activity increased. The content of GSH reduced (-20% vs controls),and activity of GSH-related enzymes was significantly inhibited.CONCLUSION: Gliadin treatment induces an imbalance in the antioxidative mechanism of cells cultured by the three-dimensional technique. This alteration may explain the cell damage directly caused by gliadin and the subsequent morphological abnormalities. | Ersilia Dolfini Luca Elli Leda Roncoroni Barbara Costa Maria Pia Colleoni Vito Lorusso Simona Ramponi Paola Braidotti Stefano Ferrero Maria Letizia Falini Maria Teresa Bardella | 2005 | World Journal of Gastroenterology2005，11，38： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员