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| 1 | Nutrient supplemented serum-free medium increases cardiomyogenesis efficiency of human pluripotent stem cells显示文摘AIM: To development of an improved p38 MAPK inhibitor-based serum-free medium for embryoid body cardiomyocyte differentiation of human pluripotent stem cells. METHODS: Human embryonic stem cells (hESC) differentiated to cardiomyocytes (CM) using a p38 MAPK inhibitor (SB203580) based serum-free medium (SB media). Nutrient supplements known to increase cell viability were added to SB medium. The ability of these supplements to improve cardiomyogenesis was evaluated by measurements of cell viability, total cell count, and the expression of cardiac markers via flow cytometry. An improved medium containing Soy hydrolysate (HySoy) and bovine serum albumin (BSA) (SupSB media) was developed and tested on 2 additional cell lines (H1 and Siu-hiPSC). Characterization of the cardiomyocytes was done by immunohistochemistry, electrophysiology and quantitative real-time reverse transcriptionpolymerase chain reaction. RESULTS: hESC cell line, HES-3, differentiating in SB medium for 16 d resulted in a cardiomyocyte yield of 0.07 ± 0.03 CM/hESC. A new medium (SupSB media) was developed with the addition of HySoy and BSA to SB medium. This medium resulted in 2.6 fold increase in cardiomyocyte yield (0.21 ± 0.08 CM/hESC). The robustness of SupSB medium was further demonstrated using two additional pluripotent cell lines (H1, hESC and Siu1, hiPSC), showing a 15 and 9 fold increase in cardiomyocyte yield respectively. The age (passage number) of the pluripotent cells did not affect the cardiomyocyte yields. Embryoid body (EB) cardiomyocytes formed in SupSB medium expressed canonical cardiac markers (sarcomeric α-actinin, myosin heavy chain and troponin-T) and demonstrated all three major phenotypes: nodal-, atrial- and ventricular-like. Electrophysiological characteristics (maximum diastolic potentials and action potential durations) of cardiomyocytes derived from SB and SupSB media were similar. CONCLUSION: The nutrient supplementation (HySoy and BSA) leads to increase in cell viability, cell yield and cardiac marker expression during cardiomyocyte differentiation, translating to an overall increase in cardiomyocyte yield. | Sherwin Ting Marti Lecina Yau-Chi Chan Hung Fat Tse Shaul Reuveny Steve KW Oh | 2013 | World Journal of Stem Cells2013，5，3： | 4 |
| 2 | Distinct regulation of mitogen-activated protein kinase activities is coupled with enhanced cardiac differentiation of human embryonic stem cells显示文摘 | Kempf H Lecina M Ting S | | 0，，： | 1 |
| 3 | A comparative study of the effect of mechanical and ultrasound agitation on the properties of electrodeposited Ni/Al2O3 nonocomposite coatings显示文摘 | Garcia - Lecina E Garcia - Urrutia I Diez J A | 2012 | Surface & Coatings Technology2012，，1112： | 1 |
| 4 | Comparison of New Tau PET-Tracer Candidates with T808 and T807显示文摘 | Declercq L Celen S Lecina J | 2016 | Mol Imaging2016，15，53： | 1 |
| 5 | Scalable platform for human embryonic stem cell differentiation to cardiomyocytes in suspended microcarrier cultures显示文摘 | Lecina M Ting S Choo A | | 0，，： | 1 |
| 6 | Codeposition of inorganic fullerene - like WS2 nanoparticles in an electrodeposited nickel matrix under the influence of ultrasonic agitation 显示文摘 | GARCA- LECINA E GARCA- URRUTIA I DfEZ J A | 2013 | Electrochimica Acta2013，114，10： | 1 |
| 7 | Fixed versus variable bulk canopy resistance for reference evapotranspiration estimation using the Penman-Monteith equation under semiarid conditions显示文摘 | Lecina S Martínez-Cob A Pérez P J | 2003 | Agricultural Water Management2003，60，： | 1 |
| 8 | A Compara-tive Study of the Effect of Mechanical and Ultrasound Agita-tion on the Properties of Electrodeposited Ni/Al203 Nano-composite Coatings 显示文摘 | LECINA E G URRUTIA I G DIEZ J A | 2012 | Surface and Coatings Technology2012，206，1112： | 1 |
| 9 | Fixed versus variable bulk canopy resistance for reference evaportanspirafion estimation using the Penman-Monteith equation under semarid condition显示文摘 | LECINA S MARTINEA-COB A PEREZ P J | 2003 | Agricultural and Water Management2003，60，3： | 1 |
| 10 | Electrochemical preparation and characterization of Ni/SiC compositionally graded multilayered Coatings 显示文摘 | GARCiA - LECINA E GARCiA - URRUTIA I DiEZ J A | 2009 | Electrochimica Acta2009，54，9： | 1 |
| 11 | Fixed versus variable bulk canopy resistance for reference evaportan- spiration estimation using the Penman-Monteith equation under semarid condition显示文摘 | Lecina S Martinez-cob A Perez P J | 2003 | Agricultural and water management2003，60，： | 1 |
| 12 | On-line monitoring of yeast cell growth by impedance spectroscopy显示文摘 | Soley A Lecina M Gamez X | 2005 | Journal of Biotechnology2005，118，： | 1 |
| 13 | A comparative study of the effect of mechanical and ultrasound agitation on the properties of electrodepos- ited Ni/A120z nanocomposite coatings 显示文摘 | GARCfA LECINA E GARCA - URRUTIA I DEZ J A | 2012 | Surface and Coatings Technology2012，206，1112： | 1 |
| 14 | Fixed versus variable bulk canopy resistance for reference evapo- transpiration estimation using the Penman-Monteith equation under semiarid conditions 显示文摘 | LECINA S MARTiNEZ-COB A PIREZ P J | 2003 | Agricultural Water Manage- ment2003，60，3： | 1 |
| 15 | Fixed versus variable bulk canopy resistance for reference evapotranspiration estimation using the Penman-Monteith equation under semiarid conditions 显示文摘 | Lecina S Martinez-Cob A Perez p J | 2003 | Agricultural Water Management2003，60，3： | 1 |
| 16 | Corrosion properties of double layer nickel coatings obtained by pulse plating techniques 显示文摘 | IMAZ N GARCIA - LECINA E DIEZ J A | 2010 | Transactions of the Institute of Metal Finishing2010，88，5： | 1 |
| 17 | Fixed versus variable bulk canopy resistance for reference evapotranspiration estimation using the Penman-Monteith equation under semiarid conditions显示文摘 | Lecina S Martnez-Cob A Prez PJ | 2003 | Agricultural Water Management2003，60，： | 1 |
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