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| 1 | Structural and molecular features of intestinal strictures in rats with Crohn's-like disease显示文摘AIM: To develop a new rat model we wanted to gain a better understanding of stricture formation in Crohn's disease(CD).METHODS: Chronic colitis was induced locally by the administration of 2,4,6-trinitrobenzenesulfonic acid(TNBS). The relapsing inflammation characteristic to CD was mimicked by repeated TNBS treatments. Animals were randomly divided into control, once, twice and three times TNBS-treated groups. Control animals received an enema of saline. Tissue samples were taken from the strictured colonic segments and also adjacent proximally and distally to its 60, 90 or 120 d after the last TNBS or saline administrations. The frequency and macroscopic extent of the strictures were measured on digital photographs. The structural features of strictured gut wall were studied by light- and electron microscopy. Inflammation related alterations in TGF-beta 2 and 3, matrix metalloproteinases 9(MMP9) and TIMP1 m RNA and protein expression were determined by quantitative real-time PCR and western blot analysis. The quantitative distribution of caspase 9 was determined by post-embedding immunohistochemistry.RESULTS: Intestinal strictures first appeared 60 d after TNBS treatments and the frequency of them increased up to day 120. From day 90 an intact lamina epithelialis, reversible thickening of lamina muscularis mucosae and irreversible thickening of the muscularis externa were demonstrated in the strictured colonic segments. Nevertheless the morphological signs of apoptosis were frequently seen and excess extracellular matrix deposition was recorded between smooth muscle cells(SMCs). Enhanced caspase 9 expression on day 90 in the SMCs and on day 120 also in myenteric neurons indicated the induction of apoptosis. The m RNA expression profile of TGF-betas after repeated TNBS doses was characteristic to CD, TGF-beta 2, but not TGF-beta 3 was up-regulated. Overexpression of MMP9 and down-regulation of TIMP1 were demonstrated. The progressive increase in the amount of MMP9 protein in the strictures was also obvious between days 90 and 120 but TIMP1 protein was practically undetectable at this time.CONCLUSION: These findings indicate that aligned structural and molecular changes in the gut wall rather than neuronal cell death play the primary role in stricture formation. | Petra Talapka Anikó Berkó Lajos István Nagy Lalitha Chandrakumar Mária Bagyánszki László Géza Puskás éva Fekete Nikolett Bódi | 2016 | World Journal of Gastroenterology2016，22，22： | 3 |
| 2 | Enhancing effect of malachite green on the development of hepatic pre-neo- plastic lesions induced by N-nitrosodiethylamine in rats 显示文摘 | Fernandes C Lalitha V S Rao K V K | 1991 | Carcinogenesis1991，12，5： | 1 |
| 3 | Influence of CdClz treatment on structural and optical properties of vacuum evapo- rated CdTe thin films 显示文摘 | Lalitha S Sathyamoorthy R Senthilarasu S | 2006 | Solar Energy Materials & Solar Cells2006，90，： | 1 |
| 4 | Primer premier 5 显示文摘 | Lalitha S | 2000 | Biotech Software &Internet Report2000，1，6： | 1 |
| 5 | Surface protection of copper in acid medium by azoles and surfactants显示文摘 | Lalitha A Ramesh S Rajeswari S | 2005 | Electrochimica Acta2005，51，： | 1 |
| 6 | Generalized B-vex functions and generalized B-vex programming显示文摘 | Bector C R and Suneja S K and Lalitha C S | 1993 | Journal of Optimization Theory and Applications1993，，： | 1 |
| 7 | Surface Protection of Copper in Acid Medium by Azoles and Surfactants显示文摘 | Lalitha A Ramesh S Rajeswari S | 2005 | Electrochimiea Acta2005，51，： | 1 |
| 8 | Enhancing effects of malachite green on development of hepatic preneoplastic lesions induced by N - ni - trosodiethylamine in rats 显示文摘 | Fernandes C Lalitha V S Rao V K | 1991 | Carcinogenesis1991，12，： | 1 |
| 9 | Structural properties of CdTe thin films on different substrates 显示文摘 | Chandramohan S Sathyamoorthy R Lalitha S | 2006 | Solar Energy Materials &Solar Cells2006，90，： | 1 |
| 10 | Activity of antibiotics against Fusar- ium and Aspergillus显示文摘 | Day S Lalitha P Haug S | 2009 | Br JOphthalmol2009，93，1： | 1 |
| 11 | Primer premier 5 显示文摘 | Lalitha S | 2000 | Biotech Software Internet Re- port2000，1，6： | 1 |
| 12 | Structural properties of CdTe thin films on different substrates 显示文摘 | Chandramohan S Sathyamoorthy R Lalitha S | 2006 | Solar Energy Materials & Solar Cells2006，90，： | 1 |
| 13 | Generalized nonsmooth invexity显示文摘 | Kaul R N Suneja S K Lalitha C S | 1994 | J Info Optim Sci1994，15，： | 1 |
| 14 | Enhancing effects of malachite green on development of hepatic preneoplastic lesions induced by N-nitrosodiethylamine in rats显示文摘 | Femandes C Lalitha V S Ran V K | 1991 | Carcinogenesis1991，12，： | 1 |
| 15 | Lipid peroxidation,osmotic fragility and antioxidant status in children with acute post-streptococcal glomerulonephritis显示文摘 | Devasena T Lalitha S Padma K | 2001 | Clin Chim Acta2001，308，： | 1 |
| 16 | Generalized B-vex functions and generalized B-vex programming显示文摘 | Bector C R Suneja S K Lalitha C S | 1993 | J Optim Theory Appl1993，76，： | 1 |
| 17 | Enhancing effects of malachite green on development of hepatic preneoplastic lesions induced by N - nitrosodiethylamine in rats 显示文摘 | Fernandes C Lalitha V S Rao V K | 1991 | Carcinogensis1991，12，： | 1 |
| 18 | Generalized B -vex functions and generalized B -vex programming显示文摘 | BECTOR C R SUNEJA S K LALITHA C S | 1993 | J Optim Theory Appl1993，76，： | 1 |
| 19 | Stability of parametric quasivariational inequality of the minty type显示文摘 | Lalitha C S Bhatia G | 2011 | Journal of Optimization Theory and Applications2011，148，： | 1 |
| 20 | Generalized B-vex Functions and Generalized B-vex Programming 显示文摘 | BECTOR C R SUNEJA S K LALITHA C S | 1993 | JOTA1993，76，3： | 1 |
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