| 1 | Determination of gemcitabine and its metabolite in human plasma using high-pressure liquid chromatography coupled with a diode array detector显示文摘AIM: To establish a high pressure liquid chromatography (HPLC) method for determination of the concentration of gemcitabine (dFdC) and its metabolite(dFdU) in human plasma. METHODS: Plasma 1.0 mL spikedwith floxuridine as an internal standard was extracted with 3.0 mL of methanol-acetonitrile (v/v, 1:9). The supernatant was evapo- rated at 60 oC and the residue was reconstituted with 0.5 mL of the solution used as the mobile phase. After centrifugation, 50 μL of the supernatant was injected into the HPLC system. Separation was achieved on a C18 (4.6 mm×250 mm, 5 μm) column at 25 oC with the flow rate of the mobile phase set to 0.8 mL/min. The compounds were detected at 268 nm. The mobile phase consisted of 40.0 mmol/L acetate ammonium buffer solution (pH 5.5) and acetonitrile (v/v, 97.5:2.5). RESULTS: The linear range was 0.20-10.0 mg/L(r=0.9999) for dFdC and 0.50-50.0 mg/L(r=0.9999) for dFdU. The limit of detection (LOD) was 0.10 mg/L for dFdC and 0.25 mg/L for dFdU, while the limit of quantification (LOQ) was 0.20 mg/L (RSD<10 %) for dFdC and 0.50 mg/L(RSD <3 %) for dFdU. The average recovery of dFdC and dFdU by this method were 103.3 % and 98.7 %, respectively. For intra-day and inter-day, the corresponding standard deviations of the measurements of dFdC and dFdU were both less than 5.5 %. CONCLUSION: An analytical method was established to measure the concentrations of dFdC and dFdU in human plasma and was effectively applied to the dFdC and dFdU pharmacokinetic studies of 8 Chinese patients with malignant tumors. | Neng-mingLIN SuZENG Sheng-linMA YunFAN Hai-junZHONG LuoFANG | 2004 | Acta Pharmacologica Sinica2004，25，12： | 4 |
| 4 | Applications of Soil Series in Sustainable Land Use of Oasis Farmlands显示文摘The issue of agricultural land sustainability is important to every part of the world, especially in the developing countries. Based on the data collected by field investigation and laboratory analysis as well as soil classification theory, the authors discuss the applications of soil series in the arid northwestern China. On the one hand, the application in the layout of rational agricultural of oasis farmlands: (1) two soil series,namely,Lanjiabao (main) and Nijiaxiaying, belong to the moderate and extremely arid region in the study area; accordingly, the layout of crops is the spring wheat and maize (main) and winter wheat;(2) Nijiaxiaying (main) and Lanjiabao, however, fall into the warm-cold and extremely arid region, where the winter wheat (main) and spring wheat grow; and (3) the Shangfusi is part of the warm chilly-arid region, which is used as seasonal pastureland. On the other hand, the application on the soil quality of oasis farmlands, the results indicating that among all soil series, the content of N is very low, however, the content of K is abundant, and the content of P is greatly scarce. | QIShan-zhong XIAOHong-lang LUOFang | 2005 | 水土保持研究2005，12，2： | 0 |
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