| 1 | Auxin response factor 6A regulates photosynthesis,sugar accumulation,and fruit development in tomato显示文摘Auxin response factors(ARFs)are involved in auxin-mediated transcriptional regulation in plants.In this study,we performed functional characterization of SlARF6A in tomato.SlARF6A is located in the nucleus and exhibits transcriptional activator activity.Overexpression of SlARF6A increased chlorophyll contents in the fruits and leaves of tomato plants,whereas downregulation of SlARF6A decreased chlorophyll contents compared with those of wild-type(WT)plants.Analysis of chloroplasts using transmission electron microscopy indicated increased sizes of chloroplasts in SlARF6A-overexpressing plants and decreased numbers of chloroplasts in SlARF6A-downregulated plants.Overexpression of SlARF6A increased the photosynthesis rate and accumulation of starch and soluble sugars,whereas knockdown of SlARF6A resulted in opposite phenotypes in tomato leaves and fruits.RNA-sequence analysis showed that regulation of SlARF6A expression altered the expression of genes involved in chlorophyll metabolism,photosynthesis and sugar metabolism.SlARF6A directly bound to the promoters of SlGLK1,CAB,and RbcS genes and positively regulated the expression of these genes.Overexpression of SlARF6A also inhibited fruit ripening and ethylene production,whereas downregulation of SlARF6A increased fruit ripening and ethylene production.SlARF6A directly bound to the SAMS1 promoter and negatively regulated SAMS1 expression.Taken together,these results expand our understanding of ARFs with regard to photosynthesis,sugar accumulation and fruit development and provide a potential target for genetic engineering to improve fruit nutrition in horticulture crops. | Yujin Yuan Xin Xu Zehao Gong Yuwei Tang Mengbo Wu Fang Yan Xiaolan Zhang Qian Zhang Fengqing Yang Xiaowei Hu Qichen Yang Yingqing Luo Lihua Mei Wenfa Zhang Cai-Zhong Jiang Wangjin Lu Zhengguo Li Wei Deng | 2019 | Horticulture Research2019，6，1： | 3 |
| 2 | Research on cognitive load evaluation with subjective method in manual assembly显示文摘Subjective scales have different kinds of applicability in diverse fields.This study intends to implement a quantitative approach to determine the applicability of subjective scales in manual as-sembly work and evaluate the cognitive load of assembly workers.A multi-scale research paradigm based on subjective evaluation method is proposed.Three typical task stages are extracted from the process of assembly work.The National Aeronautics and Space Administration Task Load Index(NASA-TLX)scale,PAAS scale and Workload Profile Index Ratings(WP)scale are selected for the design of 3×3 multi-factor mixed experiment.The power spectrum density(PSD)characteris-tics of electroencephalogram(EEG)are utilized to identify the difficulty levels of the three task sta-ges.The relevant indicators of scale applicability are assessed.The results show that in terms of sensitivity,NASA-TLX scale reaches the highest sensitivity(F=999.137,P=0<0.05).In terms of validity,NASA-TLX scale possesses the best concurrent validity(P=0.0255<0.05).In terms of diagnosticity,NASA-TLX scale based on 6 dimensions takes on the best diagnostic performance.In terms of subject acceptability,WP scale performs the worst.According to the analytic hierarchy process(AHP)model,the applicability scores of NASA-TLX scale,PAAS scale and WP scale are determined as 3,2.55 and 1.6714,respectively.Therefore,NASA-TLX scale is regarded as the most suitable subjective evaluation questionnaire for assembly workers,which is also an effective quantitative evaluation method for the cognitive load of assembly workers. | 任彬 ZHOU Qinyu LI Qibing LUO Wenfa | 2023 | High Technology Letters2023，29，4： | 0 |
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