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| 1 | New gene therapy strategies for hepatic fibrosis显示文摘The liver is the largest internal organ of the body, which may suffer acute or chronic injury induced by many factors, leading to cirrhosis and hepatocarcinoma. Cirrhosis is the irreversible end result of fibrous scarring and hepatocellular regeneration, characterized by diffuse disorganization of the normal hepatic structure, regenerative nodules and fibrotic tissue. Cirrhosis is associated with a high co-morbidity and mortality without effective treatment, and much research has been aimed at developing new therapeutic strategies to guarantee recovery. Liver-based gene therapy has been used to downregulate specific genes, to block the expression of deleterious genes, to delivery therapeutic genes, to prevent allograft rejection and to augment liver regeneration. Viral and non-viral vectors have been used, with viral vectors proving to be more efficient. This review provides an overview of the main strategies used in liver-gene therapy represented by non-viral vectors, viral vectors, novel administration methods like hydrodynamic injection, hybrids of two viral vectors and blocking molecules, with the hope of translating findings from the laboratory to the patient′s bed-side. | Adriana M Salazar-Montes Luis D Hernández-Ortega Martha S Lucano-Landeros Juan Armendariz-Borunda | 2015 | World Journal of Gastroenterology2015，21，13： | 12 |
| 2 | Recurrence of inflammatory pseudotumor in the distal bile duct: Lessons learned from a single case and reported cases显示文摘煽动性的 myofibroblastic 肿瘤(IMT ) 或煽动性的假肿瘤(IP ) 广泛地在文学被讨论了。他们通常在肺和上面的呼吸道被发现。然而,包含 biliopancreatic 区域的案例报导在最近的年增加了。限制到胰腺的头或远侧的胆汁管的这些损害的 Immunohistochemical 学习似乎与那些在称为自体免疫的胰腺炎的一个新实体观察了兼容,但是通常强烈的纤维变性反应(带状配列) 支配生产一个团。当这个条件限于胰腺的头时,胆总管可能被煽动性的过程包含,黄疸可以经常发生类似于胰的腺癌。我们以前报导了从与是一个极其稀罕的实体的自体免疫的胰腺炎联系的胆汁管产生的 IMT 的一个案例。在 Kaush-Whipple 切除术以后的四年,平淡的后续上的放射学的检查揭示了一个肿瘤团,建议本地复发。指导超声的 FNA 证实了我们的可疑诊断。作为其它,这个现在的案例建议坚持的后续是必要的以便在这个特定的地点阻止不可逆的肝损坏。 | EM López-Tomassetti Fernández H Díaz Luis A Martín Malagón I Arteaga González A Carrillo Pallarés | 2006 | World Journal of Gastroenterology2006，12，24： | 7 |
| 3 | Preclinical evaluation of azathioprine plus buthionine sulfoximine in the treatment of human hepatocarcinoma and colon carcinoma显示文摘AIM: To evaluate the efficacy and the safety of azathioprine (AZA) and buthionine sulfoximine (BSO) bylocalized application into HepG2 tumor in vivo.METHODS: Different hepatoma and colon carcinoma cell lines (HepG2, HuH7, Chang liver, LoVo, RKO, SW-48, SW-480) were grown in minimal essencial medium supplemented with 10% fetal bovine serum and 1% antibiotic/antimycotic solution and maintained in a humidified 37 ℃ incubator with 5% CO2. These cells were pretreated with BSO for 24 h and then with AZA for different times. We examined the effects of this combination on some proteins and on cellular death. We also studied the eff icacy and the safety of AZA (6 mg/kg per day) and BSO (90 mg/kg per day) in HepG2 tumor growth in vivo using athymic mice. We measured safety by serological markers such as aminotransferases and creatine kinase.RESULTS: The in vitro studies revealed a new mechanism of action for the AZA plus BSO combination in the cancer cells compared with other thiopurines (6-mercaptopurine, 6-methylmercaptopurine, 6-thioguanine and 6-methylthioguanine) in combination with BSO. The cytotoxic effect of AZA plus BSO in HepG2 cells resulted from necroptosis induction in a mitochondrial-dependent manner. From kinetic studies we suggest that glutathione (GSH) depletion stimulates c-Jun amino-terminal kinase and Bax translocation in HepG2 cells with subsequent deregulation of mitochondria (cytochrome c release, loss of membrane potential), and proteolysis activation leading to loss of membrane integrity, release of lactate dehydrogenase and DNA degradation. Some of this biochemical and cellular changes could be reversed by N-acetylcysteine (a GSH replenisher). In vivo studies showed that HepG2 tumor growth was inhibited when AZA was combined with BSO.CONCLUSION: Our studies suggest that a combination of AZA plus BSO could be useful for localizedtreatment of hepatocellular carcinoma as in the currently used transarterial chemoembolization method. | Borja Hernández-Breijo Jorge Monserrat Sara Ramírez-Rubio Eva P Cuevas Diana Vara Inés Díaz-Laviada M Dolores Fernández-Moreno Irene D Román Javier P Gisbert Luis G Guijarro | 2011 | World Journal of Gastroenterology2011，17，34： | 2 |
| 4 | Inhibition effects of nitrogen compounds on the hydrodesulfurization of dibenzothiophene显示文摘 | LAREDO S G C DE LOS REYES H J A LUIS CANO D J | 2001 | Applied Catalysis2001，207，12： | 1 |
| 5 | Chromenes of polyketide oridin from Peperomia villipetiola 显示文摘 | Karina J M S Guillermo E D P Luis R L | 2005 | Phytochemistry2005，66，： | 1 |
| 6 | Understanding the paradoxical effects of power control on the capacity of wireless networks显示文摘 | WANG Y LUI J C S CHIU D | 2009 | IEEE Trans on Wireless Communication2009，8，1： | 1 |
| 7 | DCAR: distributed coding-aware routing in wireless networks显示文摘 | LE J L LUI J C S CHIU D M | 2008 | IEEE Transantion on Mobile Computing2008，9，4： | 1 |
| 8 | Profiling and identification of P2P traffic显示文摘 | HU Y CHIU D LUI J C S | | 0，，6： | 1 |
| 9 | 显示文摘 | Luis da S Z Ronaldo D M Rogerio | 2002 | Vacuum2002，65，： | 1 |
| 10 | Influence of operating parameters on NOx and N2O axial profiles in a circulating fluidizedbedcombustor显示文摘 | Luis F D Carlos A L Xiao S | 1996 | Fuel1996，75，8： | 1 |
| 11 | Spatial shape error concealment for object-based image and video coding显示文摘 | LUIS D S FERNANDO P | 2004 | IEEE transaction on image processing2004，13，4： | 1 |
| 12 | Assessing permanent deformation resistance of high modulus asphalt mixtures 显示文摘 | CAPITAO S D LUIS P S | 2006 | Journal of Transportation Engineering2006，，5： | 1 |
| 13 | Some Heuristic Algorithms for Total Tardiness Minimization in a Flowshop with Blocking 显示文摘 | Ronconi D P Luis R S | 2009 | The International Journal of Management Science2009，37，2： | 1 |
| 14 | Optimization of the azo dye Procion Red H-EXL degradation by Fenton's reagent using experimental design 显示文摘 | Carmen S D Rodrigues Luis M Madeira Rui A R Boaventura | 2009 | Journal of Hazardous Materials2009，164，2： | 1 |
| 15 | Determination of degree of conversion as function of depth of a photo-initiated dental restoration composite显示文摘 | LUIS C MENDESA ANDREA D TEDESCOB MAURO S MIRANDAB | 2005 | Polymer Testing2005，，24： | 1 |
| 16 | Domain Decomposition Methods in Image Denoising Using Gaussian Curvature显示文摘 | Firsov D Lui S H | 2006 | Journal of Computational and Applied Mathematics2006，193，2： | 1 |
| 17 | Small-world overylay P2P networks:construction,management and handling of dynamic flash crowds显示文摘 | Hui K Y K Lui J C S Yau D K Y | 2006 | Computer Networks2006，50，15： | 1 |
| 18 | DCAR: distributed coding-aware routing in wireless networks 显示文摘 | LE Jilin LUI J C S CHIU D M | 2010 | IEEE Transactions on Mobile Computing2010，9，4： | 1 |
| 19 | Solvent-free preparation of tris-pyrazolyl-1,3,5-triazines显示文摘 | Antonio de la Hoz Angel D??az-Ortiz José Elguero Luis J Mart??nez Andrés Moreno Ana Sánchez-Migallón | 2001 | Tetrahedron2001，，20： | 1 |
| 20 | Nonpathogenic SIV infection of African green monkeys induces a strong but rapidly controlled type I IFN response显示文摘 | Jacquelin Béatrice Mayau Véronique Targat Brice Liovat Anne-Sophie Kunkel Désirée Petitjean Ga?l Dillies Marie-Agnès Roques Pierre Butor Cécile Silvestri Guido Giavedoni Luis D Lebon Pierre Barré-Sinoussi Fran?oise Benecke Arndt Müller-Trutw | 2009 | Journal of Clinical Investigation2009，，： | 1 |
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