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| 1 | A Rapid and Cost-Effective Method for Genotyping Genome-Edited Animals:A Heteroduplex Mobility Assay Using Microfluidic Capillary Electrophoresis显示文摘The recent emergence and application of engineered endonucleases have led to the development of genome editing tools capable of rapidly implementing various targeted genome editions in a wide range of species.Moreover,these novel tools have become easier to use and have resulted in a great increase of applications.Whilst gene knockout(KO) or knockin(KI) animal models are relatively easy to achieve,there is a bottleneck in the detection and analysis of these mutations.Although several methods exist to detect these targeted mutations,we developed a heteroduplex mobility assay on an automated microfluidic capillary electrophoresis system named HMA-CE in order to accelerate the genotyping process.The HMA-CE method uses a simple PCR amplification of genomic DNA(gDNA) followed by an automated capillary electrophoresis step which reveals a heteroduplexes(HD) signature for each mutation.This allows efficient discrimination of wild-type and genome-edited animals down to the single base pair level. | Vanessa Chenouard Lucas Brusselle Jean-Marie Heslan Séverine Remy Séverine Ménoret Claire Usal Laure-Hélène Ouisse Tuan Huy NGuyen Ignacio Anegon Laurent Tesson | 2016 | Journal of Genetics and Genomics2016，43，5： | 4 |
| 2 | Interaction of the major inflammatory bowel disease susceptibility alleles in Crohn’s disease patients显示文摘AIM:To investigate the interaction of interleukin-23 receptor(IL23R)(rs1004819 and rs2201841),autophagy-related 16-like 1(ATG16L1)(rs2241880), caspase recruitment domain-containing protein 15 (CARD15)genes,and IBD5 locus in Crohn's disease(CD) patients. METHODS:A total of 315 unrelated subjects with CD and 314 healthy controls were genotyped.Interactions and specific genotype combinations of a total of eight variants were tested.The variants of IBD5locus(IGR2198a_1 rs11739135 and IGR2096a_1 rs12521868),CARD15(R702W rs2066845 and L1007fs rs2066847),ATG16L1(rs2241880)and IL23R (rs1004819,rs2201841)genes were genotyped by PCR-RFLP,the G908R(rs2066844)in CARD15 was determined by direct sequencing. RESULTS:The association of ATG16L1 T300A with CD was confirmed[P=0.004,odds ratio(OR)=1.69, 95%CI:1.19-2.41],and both IL23R variants were found to represent significant risk for the disease(P= 0.008,OR=2.05,95%CI:1.20-3.50 for rs1004819 AA;P<0.001,OR=2.97,95%CI:1.65-5.33 for rs2201841 CC).Logistic regression analysis of pairwise interaction of the inflammatory bowel disease (IBD)loci indicated that IL23R,ATG16L1,CARD15 and IBD5(IGR2198a_1)contribute independently to disease risk.We also analysed the specific combina- tions by pair of individual ATG16L1,IL23R rs1004819, rs2201841,IGR2198a_1,IGR2096a_1 and CARD15 genotypes for disease risk influence.In almost all cases,the combined risk of susceptibility pairs was higher in patients carrying two different risk-associated gene variants together than individuals with just one polymorphism.The highest OR was found for IL23R rs2201841 homozygous genotype with combination of positive CARD15 status(P<0.001,OR=9.15,95% CI:2.05-40.74). CONCLUSION:The present study suggests a cumulative effect of individual IBD susceptibility loci. | Veronika Csngei Luca Járomi EnikSáfrány Csilla Sipeky Lili Magyari Bernadett Faragó Judit Bene Noémi Polgár Lilla Lakner Patrícia Sarlós Márta Varga Béla Melegh | 2010 | World Journal of Gastroenterology2010，16，2： | 2 |
| 3 | Evaluation of the inhibitory effects of human serum components on bactericidal activity of human beta-defensin-3 显示文摘 | Maisetta G Di Luca M Esin S | 2008 | Peptides2008，29，1： | 1 |
| 4 | Haematopoietic stem cell release is regulated by circadian os- cillations显示文摘 | MENDEZ-FERRER S LUCAS D BATTISTA M et ai | 2008 | Nature2008，452，7186： | 1 |
| 5 | Icdar 2005 text locating competition results显示文摘 | Lucas S M | 2005 | Pattern Recognition2005，38，： | 1 |
| 6 | HIV-1 tat elongates the G1 phase and indirectly promotes HIV-1 gene expression in cells of glial origin显示文摘 | Kundu M Sharma S De Luca A | 1998 | J Biol Chem1998，273，： | 1 |
| 7 | Icdar2003 robust reading competitions 显示文摘 | Lucas S M Panaretos A Sosa L | 2003 | Pattern Recognition2003，36，： | 1 |
| 8 | 查看详情显示文摘 | Marco de Lucas M C Fabreguette F Linsavanh M Imhoff L Heintz O Josse-Courty C Mesnier M T Potin V Bourgeois S Sacilotti M | | 0，，： | 1 |
| 9 | Exponentially weighted mov- ing average control schemes:properties and enhancements显示文摘 | LUCAS J M SACCUCCI M S | 1990 | Technometrics1990，32，1： | 1 |
| 10 | In Vitro Testing of a Pulsatile Delivery System and Its in Vivo Application for Immunization Against Tetanus Toxoid显示文摘 | CARDAMONE M LOFTHOUSE S A LUCAS J C | 1997 | J Controlled Release1997，47，3： | 1 |
| 11 | 显示文摘 | Nagorka M S Levi C G Lucas G E | 1991 | Materials Science Engineering A1991，142，2： | 1 |
| 12 | Expression of MCAM/MUC18 by human melanoma cells leads to increased tumor growth and metastasis显示文摘 | Xie S Luca M Huang S | 1997 | Cancer Res1997，57，11： | 1 |
| 13 | Loss of AP-2 results in downregulatiou of c-KIT and enhancement of melanoma tumorigenicity and metastasis显示文摘 | Huang S Jean D Luca M | | 0，，15： | 1 |
| 14 | Synthetic dyes decolourisation by White-rot fungi: development of original microtitre plate method and screening 显示文摘 | M Lucas V Mertens A-M Corbisier S Vanhulle | 2008 | Enzyme Microb Technol2008，42，2： | 1 |
| 15 | Crosscultural variations in predictors of life satisfaction : Perspectives from needs and values显示文摘 | OISHI S DIENER E F LUCAS R E SUH E M | 1999 | Personality and Social Psychology Bulletin1999，25，： | 1 |
| 16 | MiRNA expression patterns of Triticum dicoccoides in response to shock drought stress 显示文摘 | KANTAR M LUCAS S J BUDAK H | 2011 | Planta2011，233，3： | 1 |
| 17 | The draft genome of watermelon (Citrullus lanatus ) and reseqnencing of 20 diverse accessions显示文摘 | Guo S G Zhang J G Sun H H Salse J Lucas W J Zhang H Y Zheng Y Mao L Y Ren Y WangZ W Min J M Guo X S Murat F Ham B K Zhang Z L Gao S Huang M Xu Y M Zhong S L Bombarely A Mueller L Zhao H He H Q Zhang Y Zhang Z H Huang S W Tan T Pang E Lin K Hu Q Kuang H H Ni P X Wang B Liu J G Kou Q H Hou W J Zou X H Jiang J Gong G Y Klee K Schoof H Huang Y Hu X S Dong S S Liang D Q Wang J Wu K Xia Y Zhao X Zheng Z Q Xing M Liang X M Huang B Q Lv T Wang J Y Yin Y Yi H P Li R Q Wu M Z Levi A Zhang X P Giovannoni J Wang J Li YF Fei Z J Xu Y | 2013 | Nature genetics2013，45，1： | 1 |
| 18 | Exponentially weighted moving avez:age control schemes with variable sam- pling intervals 显示文摘 | SACCUCCI M S AMIN R W LUCAS J M | 1992 | Communications in Statistics-Simulation and Computation1992，21，3： | 1 |
| 19 | Exponentially weighted moving average control schemes: properties and enhance- ment显示文摘 | Lucas J M Saccucci M S | 1990 | Technometrics1990，32，1： | 1 |
| 20 | Evolution of the structure of a gas-liquid two-phase flow in a large vertical pipe 显示文摘 | Prasser H M Beyer M Carl H Gregor S Lucas D Pietruske H Schutz P Weiss F P | 2007 | Nuclear Engineering and Design2007，237，： | 1 |
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