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| 1 | IPA1 functions as a downstream transcription factor repressed by D53 in strigolactone signaling in rice显示文摘Strigolactones (SL ) ,一组类胡萝卜素导出 terpenoid 内酯,是压制射击由禁止腋的芽的长出分叉的 root-to-shoot 植物激素。矮子 53 (D53 ) ,表明小径的 SL 的关键抑压者,被推测调整 SL 反应的下游的 transcriptional 网络。然而, D53 指向的下游的抄写因素还都没被报导。这里,我们报导那理想的植物体系结构 1 (IPA1 ) ,在米饭的植物体系结构的一个关键管理者,在调整 tiller 数字和导致 SL 的基因表示作为 D53 的一个直接下游的部件工作。我们证明 D53 在 vivo 并且在 vitro 与 IPA1 交往并且压制 IPA1 的 transcriptional 激活活动。我们进一步证明 IPA1 能直接绑在 D53 倡导者并且在导致 SL 的 D53 表示的反馈规定起一个关键作用。这些调查结果表明 IPA1 是可能的与 D53 行动到的长推测的抄写因素之一调停在米饭的调整 SL 的 tiller 发展。 | Xiaoguang Song Zefu Lu Hong Yu Gaoneng Shao Jinsong Xiong Xiangbing Meng Yanhui Jing Guifu Liu Guosheng Xiong Jingbo Duan Xue-Feng Yao Chun-Ming Liu Hongqing Li Yonghong Wang Jiayang Li | 2017 | Cell Research2017，27，9： | 48 |
| 2 | Apoptosis during β-mercaptoethanol-induced differentiation of adult adipose-derived stromal cells into neurons显示文摘β-mercaptoethanol can induce adipose-derived stromal cells to rapidly and efficiently differentiate into neurons in vitro.However,because of the short survival time of the differentiated cells,clinical applications for this technique are limited.As such,we examined apoptosis of neurons differentiated from adipose-derived stromal cells induced with β-mercaptoethanol in vitro using terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling and transmission electron microscopy.The results revealed that the number of surviving cells decreased and apoptosis rate increased as induction time extended.Taken together,these results suggest that apoptosis occurring in the process of adipose-derived stromal cells differentiating into neurons is the main cause of cell death.However,the mechanism underlying cellular apoptosis should be researched further to develop methods of controlling apoptosis for clinical applications. | Yanan Cai Xiaodong Yuan Ya Ou Yanhui Lu | 2011 | Neural Regeneration Research2011，6，10： | 19 |
| 3 | Using Fuzzy Theory and Variable Weights for Water Quality Evaluation in Poyang Lake, China显示文摘Achieving water purity in Poyang Lake has become a major concern in recent years, thus appropriate evaluation of spatial and temporal water quality variations has become essential. Variations in 11 water quality parameters from 15 sampling sites in Poyang Lake were investigated from 2009 to 2012. An integrative fuzzy variable evaluation(IFVE) model based on fuzzy theory and variable weights was developed to measure variations in water quality. Results showed that: 1) only chlorophyll-a concentration and Secchi depth differed significantly among the 15 sampling sites(P < 0.01), whereas the 11 water quality parameters under investigation differed significantly throughout the seasons(P < 0.01). The annual variations of all water quality variables except for temperature, electrical conductivity, suspended solids and total phosphorus were considerable(P < 0.05). 2) The IFVE model was reasonable and flexible in evaluating water quality status and any possible ′bucket effect′. The model fully considered the influences of extremely poor indices on overall water quality. 3) A spatial analysis indicated that anthropogenic activities(particularly industrial sewage and dredging) and lake bed topography might directly affect water quality in Poyang Lake. Meanwhile, hydrological status and sewage discharged into the lake might be responsible for seasonal water quality variations. | LI Bing YANG Guishan WAN Rongrong ZHANG Lu ZHANG Yanhui DAI Xue | 2017 | Chinese Geographical Science2017，27，1： | 15 |
| 4 | Tiller Bud Formation Regulators MOC1 and MOC3 Cooperatively Promote Tiller Bud Outgrowth by Activating F0N1 Expression in Rice显示文摘Tillering in rice is one of the most important agronomic traits.Rice tiller development can be divided into two main processes: the formation of the axillary bud and its subsequent outgrowth.Several genes critical for bud formation in rice have been identified by genetic studies;however,their molecular functions and relationships are still largely unknown.Here,we report that MONOCULM 1 (MOC1) and MONOCULM 3/ TILLERS ABSENT 1/STERILE AND REDUCED TILLERING 1 (MOC3/TAB1/SRT1),two vital regulators for tiller formation in rice,physically interact to regulate tiller bud outgrowth through upregulating the expression of FLORAL ORGAN NUMBER 1 (FON1),the homolog of CLAVATA1 in rice.We found that M0C3 is able to directly bind the promoter ofFONI and subsequently activate FON1 expression.MOC1 functions as a coactivator of MOC3,whereas it could not directly bind the FON1 promoter,and further activated FON1 expression in the presence of MOC3.Accordingly,FON1 is highly expressed at axillary meristems and shows remarkably decreased expression levels in mod and moc3 mutants.Loss-of-function mutants of FON1 exhibit normal bud formation but defective bud outgrowth and reduced tiller number.Collectively,these results shed light on a joint transcriptional regulatory mechanim by MOC1 and MOC3,and establish a new framework for the control of tiller bud formation and outgrowth. | Gaoneng Shao Zefu Lu Jinsong Xiong Bing Wang Yanhui Jing Xiangbing Meng Guifu Liu Haiyan Ma Yan Liang Fan Chen Yonghong Wang Jiayang Li Hong Yu | 2019 | Molecular Plant2019，12，8： | 15 |
| 5 | A novel ethanol-based method to induce differentiation of adipose-derived stromal cells into astrocytes显示文摘The quantity and survival time of astrocytes,which were differentiated from adult adipose-derived stromal cells after exposure to an inducer containing 3-isobutyl-1-methylxanthine,have thus far been unsatisfactory.The present study investigated the growth and differentiation characteristics of induced astrocytes by observing their growth curves.After induction for 48 hours with an inducer containing 0.5% ethanol,some adult adult adipose-derived stromal cells displayed typical astrocytic morphology.The cell quantity gradually decreased with prolonged induction time.Nestin,glial fibrillary acidic protein,and S-100 expression reached peak levels at 14 days,but neuron-specific enolase was not expressed.These results suggest that the induced astrocytes reached their peak at 14 days.Further optimization of the culture environment may yield mature astrocytes with normal functions,in greater quantity,and prolonged survival time. | Ya Ou Xiaodong Yuan Yanan Cai Yanhui Lu | 2011 | Neural Regeneration Research2011，6，10： | 12 |
| 6 | Modes of Grain Selection in Spiral Selector during Directional Solidification of Nickel-base Superalloys显示文摘The modes of grain selection in spiral selector were investigated by both a ProCAST simulation and experimental confirmation.The results show that the efficiency of grain selection in starter block is associated with the geometry shape.At the early stage of grain selection,the optimization of grain orientation is dominated by competitive grain growth,but the optimization of grain orientation in starter block is gradually dominated by geometry shape at the later stage of grain selection.Besides,the spiral part could also optimize the orientation of the selected single crystal when the initial angle is large enough,and the single crystal selection in spiral parts with different pitch lengths and initial angles is dominated by different modes.The simulation results agree well with experimental ones. | Xiangbin Meng Qi Lu Jinguo Li Tao Jin Xiaofeng Sun Jun Zhang ZhongqiangChen Yanhui Wang Zhuangqi Hu | 2012 | Journal of Materials Science & Technology2012，28，3： | 8 |
| 7 | Boosting electrocatalytic water splitting via metal-metalloid combined modulation in quaternary Ni-Fe-P-B amorphous compound显示文摘Design and synthesis of highly efficient and cost-effective bifunctional catalysts for hydrogen evolution reaction(HER)and oxygen evolution reaction(OER)remain a big challenge.Herein,a quaternary amorphous nanocompound Ni-Fe-P-B has been synthesized by a facile,scalable co-reduction method.The Ni-Fe-P-B exhibits high electrocatalytic activity and outstanding durability for both HER and OER,delivering a current density of 10 mA·cm^-2 at overpotentials of 220 and 269 mV,respectively.When loaded on carbon fiber paper(CFP)as a bifunctional catalyst,the Ni-Fe-P-B@CFP electrode requires a low cell voltage of 1.58 V to obtain 10 mA·cm^-2 for overall water splitting with negligible recession over 60 h.The excellent catalytic performances of Ni-Fe-P-B mainly benefit from the metal-metalloid combined composition modulation and the unique amorphous structure.This work provides new insights into the design of robust bifunctional catalysts for water splitting,and may promote the development of multicomponent amorphous catalysts. | Wukui Tang Xiaofang Liu Ya Li Yanhui Pu Yao Lu Zhiming Song Qiang Wang Ronghai Yu Jianglan Shui | 2020 | Nano Research2020，13，2： | 8 |
| 8 | Neural substrates of data-driven scientific discovery:An fMRI study during performance of number series completion task显示文摘Although much has been known about how humans psychologically perform data-driven scientific discovery,less has been known about its brain mechanism.The number series completion is a typical data-driven scientific discovery task,and has been demonstrated to possess the priming effect,which is attributed to the regularity identification and its subsequent extrapolation.In order to reduce the heterogeneities and make the experimental task proper for a brain imaging study,the number magnitude and arithmetic operation involved in number series completion tasks are further restricted.Behavioral performance in Experiment 1 shows the reliable priming effect for targets as expected.Then,a factorial design (the priming effect:prime vs.target;the period length:simple vs.complex) of event-related functional magnetic resonance imaging (fMRI) is used in Experiment 2 to examine the neural basis of data-driven scientific discovery.The fMRI results reveal a double dissociation of the left DLPFC (dorsolateral prefrontal cortex) and the left APFC (anterior prefrontal cortex) between the simple (period length=1) and the complex (period length=2) number series completion task.The priming effect in the left DLPFC is more significant for the simple task than for the complex task,while the priming effect in the left APFC is more significant for the complex task than for the simple task.The reliable double dissociation may suggest the different roles of the left DLPFC and left APFC in data-driven scientific discovery.The left DLPFC (BA 46) may play a crucial role in rule identification,while the left APFC (BA 10) may be related to mental set maintenance needed during rule identification and extrapolation. | ZHONG Ning LIANG PeiPeng QIN YuLin LU ShengFu YANG YanHui LI KunCheng | 2011 | Science China(Life Sciences)2011，54，5： | 8 |
| 9 | Major and trace element composition of surface sediments from the Southwest Indian Ridge:evidence for the incorporation of a hydrothermal component显示文摘在深海的沉积的热水的材料在海中央山脉提供柔韧的 tracer 给局部性的热水的活动。为从散布西南印度理兹的 ultraslow 收集的表面沉积的专业,踪迹和稀土元素元素(REE ) 数据被介绍检验热水的部件的存在。源於生物的碳酸盐淤泥统治所有沉积样品,与在一个不稳定免费的基础上从 85.5% ~ 89.9% 变化的 CaO 内容。由~的体积沉积的沥滤的残余5% HCl 在 SiO 2,艾尔 2 O 3, CaO , MgO ,碱元素( Rb , C )并且高地力量元素( Nb , Ta , Zr , Hf , Ti )。这些招待岩屑的元素被推断显著地借助于 eolian 灰尘从澳大利亚的大陆被发源,当本地 volcaniclastics 的贡献是不足道的时。另外,剩余部分与 UCC 相比在 Fe, Mn,和 Ba 显示出清楚的丰富。联合反对 UCC 的剩余部分的积极 Eu 异例但是热水的液体特征、联系猛抛发生在海中央山脉,局部性的热水的部件的加入能被抑制。多于在剩余部分(~55%60%) 以内的半 REE 从一个热水的部件被导出, REE 混合计算显示那,它被推断从一个弥漫的液体矿化作用被结果。低温度的弥漫的流动可以广泛地沿着散布外壳的差错和裂缝丰富的山脉的 slow-ultraslow 被散布,并且可能有一个大矿化作用潜力。 | LI Zhenggang CHU Fengyou JIN Lu LI Xiaohu DONG Yanhui CHEN Ling ZHU Jihao | 2016 | Acta Oceanologica Sinica2016，35，2： | 8 |
| 10 | Adult adipose-derived stromal cells differentiate into neurons with normal electrophysiological functions显示文摘β-mercaptoethanol was used to induce in vitro neuronal differentiation of adipose-derived stromal cells. Within an 8-hour period post-differentiation, the induced cells exhibited typical neuronal morphology, and expression of microtubule-associated protein 2 and neuron-specific enolase, which are markers of mature neurons, reached a peak at 5 hours. Specific organelle Nissl bodies of neurons were observed under transmission electron microscopy. Results of membrane potential showed that fluorescence intensity of cells was greater after 5 hours than adipose-derived stromal cells prior to induction. In addition, following stimulation with high-concentration potassium solution, fluorescence intensity increased. These experimental findings suggested that neurons differentiated from adipose-derived stromal cells and expressed mature K+ channels. In addition, following stimulation with high potassium solution, the membrane potential depolarized and fired an action potential, confirming that the induced cells possessed electrophysiological functions. | Xiaodong Yuan Yanan Cai Ya Ou Yanhui Lu | 2011 | Neural Regeneration Research2011，6，34： | 8 |
| 11 | 肠系膜上静脉血管鞘内入路在右半结肠切除术中的临床研究(英文)显示文摘Objective: The aim of the study was to investigate the clinical value of superior mesenteric vascular intrathecal approach in right hemicolectomy. Methods: We retrospectively studied the clinical data of 132 patients who had right hemicolectomy from June 2007 to June 2010, including 68 cases with superior mesenteric vascular intrathecal approach to resect specimen, and compared the operation time, blood loss, hospital stay and the number of dissected lymph nodes with patients treated with conventional surgery. Results: Compared the vascular intrathecal approach with conventional approach, the operation time and blood loss were decreased significantly, the number of Dukes C No. 3 lymph node dissection was increased, while the incidence of postoperative complications and hospital stay were equivalent to traditional surgery group. Conclusion: The use of vascular intrathecal approach in right hemicolectomy can significantly shorten the operation time and reduce bleeding and improve surgical radical outcomes. | Yuzhou Zhao, Guangsen Han, Yingkun Ren, Yanhui Gu, Chaomin Lu Department of General Surgery, Henan Tumor Hospital Affiliated Tumor Hospital, Zhengzhou University, Zhengzhou 450003, China | 2010 | The Chinese-German Journal of Clinical Oncology2010，9，11： | 6 |
| 12 | Autophagy and apoptosis during adult adipose-derived stromal cells differentiation into neuron-like cells in vitro显示文摘β-mercaptoethanol can induce adult adipose-derived stromal cells to rapidly and efficiently differentiate into typical neuron-like cells in vitro.Immunohistochemistry showed that neuron specific enolase and neurofilament-200 expression gradually increased with the extension of induction time,and peaked at 5 hours.By contrast,glial fibrillary acidic protein was negatively expressed at all time points.Induced cells possessed a typical Nissl body,apoptosis showing condensed chromatin in the nucleus,autophagosomes with a bilayered membrane and autolysosomes in the cytoplasm at 5 hours.TUNEL assay and immunohistochemistry and immunofluorescence demonstrated that apoptosis and caspase-3 expression increased and peaked at 8 hours.Immunohistochemistry and immunofluorescence showed that microtubule-associated protein light chain 3 gradually increased with induction and reached a peak at 5 hours.These results indicate that autophagy played an important role in protecting cells during adult adipose-derived stromal cells differentiation into neuron-like cells in vitro. | Yanhui Lu Xiaodong Yuan Ya Ou Yanan Cai Shujuan Wang Qiaoyu Sun Wenli Zhang | 2012 | Neural Regeneration Research2012，7，16： | 5 |
| 13 | Synergy between metallic components of MoNi alloy for catalyzing highly efficient hydrogen storage of MgH2显示文摘Catalysts play a critical role in improving the hydrogen storage kinetics in Mg/MgH2 system.Exploring highly efficient catalysts and catalyst design principles are hot topics but challenging.The catalytic activity of metallic elements on dehydrogenation kinetics generally follows a sequence of Ti>Nb>Ni>V>Co>Mo.Herein,we report a highly efficient alloy catalyst composed of low-active elements of Mo and Ni(i.e.MoNi alloy)for MgH2 particles.MoNi alloy nanoparticles show excellent catalytic effect,even outperforming most advanced Ti-based catalysts.The synergy between Mo and Ni elements can promote the break of Mg-H bonds and the dissociation of hydrogen molecules,thus significantly improves the kinetics of Mg/MgH2 system.The MoNi-catalyzed Mg/MgH2 system can absorb and release 6.7 wt.%hydrogen within 60 s and 10 min at 300℃,respectively,and exhibits excellent cycling stability and low-temperature hydrogen storage performance.This study provides a strategy for designing efficient catalysts for hydrogen storage materials using the synergy of metal elements. | Meng Chen Yanhui Pu Zhenyang Li Gang Huang Xiaofang Liu Yao Lu Wukui Tang Li Xu Shuangyu Liu Ronghai Yu Jianglan Shui | 2020 | Nano Research2020，13，8： | 5 |
| 14 | Structural insights into a dual-specificity histone demethylase ceKDM7A from Caenorhabditis elegans显示文摘Histone 离氨酸 methylation 能被 JmjC 包含域的蛋白质在 sequence-state-specific 和 methylation-state-specific 举止移开。然而,底层特性怎么样,决定,酶怎么被调整,大部分是未知的。我们最近发现了那 ceKDM7A,一个哲学博士 -- 并且 JmjC 包含域的蛋白质,是为 H3K9me2 特定的 histone demethylase, H3K27me2,和 PHD 摸绑定到 H3K4me3 指南在 vivo 的 demethylation 活动。为了为酶的活动和 PHD 的功能提供结构的卓见进分子的机制,摸,我们与包含 H3K4me3, H3K9me2,和 H3K27me2 修正的各种各样的联合的单身者或二肽一起在 apo 形式并且在建筑群解决了酶的六水晶结构。结构显示 H3K9me2 和 H3K27me2 以一种类似的方式与 ceKDM7A 交往,并且肽绑定特性被特定的相互作用的一个网络决定。结构的几何测量也揭示了与 PHD 手指和 H3K9me2 界限联系到 JmjC 领域的那 H3K4me3 从二个分开的分子,建议 trans-histone 肽绑定机制。因此,我们的全身的结构的研究重要地由催化领域而且更多揭示底层识别不仅,为 H3K9me2 和 H3K27me2 的 ceDKM7A 的双特性的分子的机制。 | Ying Yang Lulu Hu Ping Wang Haifeng Hou Yan Lin Yi Liu Ze Li Rui Gong Xiang Feng Lu Zhou Wen Zhang Yuhui Dong Huirong Yang Hanqing Lin Yiqin Wang Charlie Degui Chen Yanhui Xu | 2010 | Cell Research2010，20，8： | 3 |
| 15 | ILF2 cooperates with E2F1 to maintain mitochondrial homeostasis and promote small cell lung cancer progression显示文摘Objective:Mitochondria play multifunctional roles in carcinogenesis.Deciphering uncertainties of molecular interactions within mitochondria will promote further understanding of cancer.Interleukin enhancer binding factor 2(ILF2)is upregulated in several malignancies,however,much remains unknown regarding ILF2 in small cell lung cancer(SCLC).In the current study,we explored ILF2's role in SCLC and demonstrated its importance in mitochondria quality control.Methods:Colony formation,cell proliferation,cell viability and xenograft studies were performed to examine ILF2's role on SCLC progression.Glucose uptake,lactate production,cellular oxygen consumption rate and extracellular acidification rate were measured to examine the effect of ILF2 on glucose metabolism.RNA-sequencing was utilized to explore genes regulated by ILF2.E2 F1 transcriptional activity was determined by dual luciferase reporter assay.Mitochondria quantification and mitochondrial membrane potential assays were performed to examine mitochondrial quality.Gene expression was determined by RT-qPCR,Western blot and IHC assay.Results:ILF2 promotes SCLC tumor growth in vitro and in vivo.ILF2 elevates oxidative phosphorylation expression and declines glucose intake and lactate production.Genome-wide analysis of ILF2 targets identified a cohort of genes regulated by E2 F1.In consistent with this,we found ILF2 interacts with E2 F1 in SCLC cells.Further studies demonstrated that suppression of E2 F1 expression could reverse ILF2-induced tumor growth and enhanced mitochondria function.Significantly,expression of ILF2 is progressively increased during SCLC progression and high ILF2 expression is correlated with higher histologic grades,which indicates ILF2's oncogenic role in SCLC.Conclusions:Our results demonstrate that ILF2 interacts with E2 F1 to maintain mitochondria quality and confers SCLC cells growth advantage in tumorigenesis. | Meng Zhao Yahui Liu Jiao Chang Jin Qi Ran Liu Yongwang Hou Yanhui Wang Xinwei Zhang Lu Qiao Li Ren | 2019 | Cancer Biology & Medicine2019，16，4： | 3 |
| 16 | Unexpected fast radical production emerges in cool seasons:implications for ozone pollution control显示文摘Ozone is a crucial air pollutant that damages human health and vegetation.As it is related to the photo-oxidation of the nitrogen oxides and volatile organic compounds,the summertime reduction of these precursors is the primary focus of current ozone mitigation strategies.During ozone pollution episodes in eastern China,an observed accumulation of daily total oxidants(O_(x)=NO_(2)+O_(3))in cool seasons(spring and autumn:60 ppb and winter 40 ppb)is comparable to that in summer(60 ppb),indicating fast photochemical production of secondary pollutants including ozone over the year.Unrecognized fast radical primary productions are found to counteract the increased termination of hydroxyl radical and unfavorable meteorological conditions to maintain the rapid total oxidant formations in cool seasons.Elucidating and regulating the primary radical sources may be critical for the secondary air pollution control in cool seasons. | Hongli Wang Yanhui Liu Xiaorui Chen Yaqin Gao Wanyi Qiu Shengao Jing Qian Wang Shengrong Lou Peter MEdwards Cheng Huang Keding Lu | 2022 | National Science Open2022，1，2： | 2 |
| 17 | Low phosphorus promotes NSP1–NSP2 heterodimerization to enhance strigolactone biosynthesis and regulate shoot and root architecture in rice显示文摘Phosphorus is an essential macronutrient for plant development and metabolism,and plants have evolved ingenious mechanisms to overcome phosphate(Pi)starvation.However,the molecular mechanisms underlying the regulation of shoot and root architecture by low phosphorus conditions and the coordinated utilization of Pi and nitrogen remain largely unclear.Here,we show that Nodulation Signaling Pathway 1(NSP1)and NSP2 regulate rice tiller number by promoting the biosynthesis of strigolactones(SLs),a class of phytohormones with fundamental effects on plant architecture and environmental responses.We found that NSP1 and NSP2 are induced by Oryza sativa PHOSPHATE STARVATION RESPONSE2(OsPHR2)in response to low-Pi stress and form a complex to directly bind the promoters of SL biosynthesis genes,thus markedly increasing SL biosynthesis in rice.Interestingly,the NSP1/2–SL signaling module represses the expression of CROWN ROOTLESS 1(CRL1),a newly identified early SL-responsive gene in roots,to restrain lateral root density under Pi deficiency.We also demonstrated that GR24^(4DO) treatment under normal conditions inhibits the expression of OsNRTs and OsAMTs to suppress nitrogen absorption but enhances the expression of OsPTs to promote Pi absorption,thus facilitating the balance between nitrogen and phosphorus uptake in rice.Importantly,we found that NSP1p:NSP1 and NSP2p:NSP2 transgenic plants show improved agronomic traits and grain yield under low-and medium-phosphorus conditions.Taken together,these results revealed a novel regulatory mechanism of SL biosynthesis and signaling in response to Pi starvation,providing genetic resources for improving plant architecture and nutrient-use efficiency in low-Pi environments. | Kun Yuan Hao Zhang Chaoji Yu Nan Luo Jijun Yan Shuang Zheng Qingliang Hu Dahan Zhang Liquan Kou Xiangbing Meng Yanhui Jing Mingjiang Chen Xinwei Ban Zongyun Yan Zefu Lu Jian Wu Yu Zhao Yan Liang Yonghong Wang Guosheng Xiong Jinfang Chu Ertao Wang Jiayang Li Bing Wang | 2023 | Molecular Plant2023，16，11： | 2 |
| 18 | miR-495 andmiR-5688 are down-regulated in non-small cell lung cancer under hypoxia to maintain interleukin-11 expression显示文摘Background:Hypoxia is a hallmark of cancer and is associated with poor prognosis.However,the molecular mechanism by which hypoxia promotes tumor progression remains unclear.MicroRNAs dysregulation has been shown to play a critical role in the tumor and tumor microenvironment.Here,we investigated the roles ofmiR-495 and miR-5688 in human non-small cell lung cancer(NSCLC)and their underlying mechanism.Methods:The expression levels of miR-495 and miR-5688 in human NSCLC tissue specimens were measured by quantitative real-time polymerase chain reaction(qRT-PCR).Deferoxamine(DFO)was used to determine whether the regulation of miR-495 and miR-5688 under hypoxia was dependent on hypoxia-inducible factor 1-alpha(HIF-1α).Furthermore,the functions of miR-495 and miR-5688 in tumor progression were evaluated using colony formation,3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2Htetrazolium(MTS),wound healing,transwell assays,and xenograft model.Two algorithms,PicTAR and Targetscan,were used to predict the target gene of these two miRNAs,and dual-luciferase reporter assay was conducted to confirm the target.The unpaired two-tailed t test,Pearson correlation analysis,and Fisher’s exact probability test were performed for statistical analyses.Results:Two miRNAs,miR-495 and miR-5688,were found to participate in NSCLC progression under hypoxia.They were down-regulated in NSCLC tissues compared with normal tissues.We determined that hypoxia led to the down-regulation of miR-495 and miR-5688 in NSCLC cells,which was independent of HIF-1αand cellular metabolic energy.In addition,miR-495 and miR-5688 suppressed cell proliferation,migration,and invasion in vitro.The NSCLC xenograft model showed that miR-495 and miR-5688 inhibited tumor formation in vivo.Interestingly,we found that miR-495 and miR-5688 had the same target,interleukin-11(IL-11).Recombinant human IL-11 counteracted the effects of miR-495 and miR-5688 on NSCLC cells,suggesting that miR-495 and miR-5688 executed their tumor suppressive role by repressing IL-11 expression.Conclusion:We found that hypoxia down-regulated the expression levels of miR-495 and miR-5688 in NSCLCto enhance IL-11 expression and tumor progression,indicating that the miR-495/miR-5688/IL-11 axismay serve as a therapeutic target and potential biomarker for NSCLC. | Meng Zhao Jiao Chang Ran Liu Yahui Liu Jin Qi Yanhui Wang Xinwei Zhang Lu Qiao Yu Jin Haohua An Li Ren | 2020 | Cancer Communications2020，40，9： | 2 |
| 19 | Hydrogen production via chemical looping reforming of coke oven gas显示文摘Coke oven gas(COG)is one of the most important by-products in steel industry,and the conversion of COG to value-added products has attracted much attention from both economic and environmental views.In this work,we use the chemical looping reforming technology to produce pure H_(2) from COG.A series of La1-xSrxFeO_(3)(x?0,0.2,0.3,0.4,0.5,0.6)perovskite oxides were prepared as oxygen carriers for this purpose.The reduction behaviors of La1-xSrxFeO_(3) perovskite by different reducing gases(H_(2),CO,CH4 and the mixed gases)are investigated to discuss the competition effect of different components in COG for reacting with the oxygen carriers.The results show that reduction temperatures of H_(2) and CO are much lower than that of CH4,and high temperatures(>800℃)are requested for selective oxidation of methane to syngas.The co-existence of CO and H_(2) shows weak effect on the equilibrium of methane conversion at high temperatures,but the oxidation of methane to syngas can inhibit the consumption of CO and H_(2).The doping of suitable amounts of Sr in LaFeO_(3) perovskite(e.g.,La0.5Sr0.5FeO_(3))significantly promotes the activity for selective oxidation of methane to syngas and inhibits the formation of carbon deposition,obtaining both high methane conversion in the COG oxidation step and high hydrogen yield in the water splitting step.The La0.5Sr0.5FeO_(3) shows the highest methane conversion(67.82%),hydrogen yield(3.34 mmol g^(-1))and hydrogen purity(99.85%).The hydrogen yield in water splitting step is treble as high as the hydrogen consumption in reduction step.These results reveal that chemical looping reforming of COG to produce pure H_(2) is feasible,and an O_(2)-assistant chemical looping reforming process can further improves the redox stability of oxygen carrier. | Kun Yang Zhenhua Gu Yanhui Long Shen Lin Chunqiang Lu Xing Zhu Hua Wang Kongzhai Li | 2021 | Green Energy & Environment2021，6，5： | 2 |
| 20 | MODELING RADIO RESOURCE MANAGEMENT IN COGNITIVE RADIO SYSTEM BASED ON OFDMA USING COLORED PETRI NET显示文摘Cognitive Radio(CR) system based on Orthogonal Frequency Division Multiple Access(OFDMA),such as Wireless Regional Area Networks(WRAN) and Worldwide Interoperability for Microwave Access(WiMAX),often attempt to improve performance via dynamic radio resource management,which is characterized as concurrent processing of different traffic and nondeterministic system capacity.It is essential to design and evaluate such complex system using proper modeling and analysis tools.In the previous work,most of the communication systems were modeled as Markov Chain(MC) and Stochastic Petri Nets(SPN),which have the explicit limitation in evaluating adaptive OFDMA CR system with wide area traffic.In this paper,we develop an executable top-down hier-archical Colored Petri Net(CPN) model for adaptive OFDMA CR system,and analyze its performance using CPN tools.The results demonstrate that the CPN can model different radio resource manage-ment algorithms in CR Systems,and the CPN tools require less computational effort than Markov model using Matlab,with its flexibility and adaptability to the traffics which arrival interval and processing time are not exponentially distributed. | Lu Yanhui Zhang Yuqiong Yang Shouyi Mu Xiaomin | 2010 | Journal of Electronics(China)2010，27，5： | 2 |
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