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| 1 | New insights into developmental biology of Eimeria tenella revealed by comparative analysis of mRNA N6-methyladenosine modification between unsporulated oocysts and sporulated oocysts显示文摘Evidence showed that N6-methyladenosine(m^(6)A)modification plays a pivotal role in influencing RNA fate and is strongly associated with cell growth and developmental processes in many species.However,no information regarding m^(6)A modification in Eimeria tenella is currently available.In the present study,we surveyed the transcriptome-wide prevalence of m^(6)A in sporulated oocysts and unsporulated oocysts of E.tenella.Methylated RNA immunoprecipitation sequencing(MeRIP-seq)analysis showed that m^(6)A modification was most abundant in the coding sequences,followed by stop codon.There were 3,903 hypermethylated and 3,178 hypomethylated mRNAs in sporulated oocysts compared with unsporulated oocysts.Further joint analysis suggested that m^(6)A modification of the majority of genes was positively correlated with mRNA expression.The mRNA relative expression and m^(6)A level of the selected genes were confirmed by quantitative reverse transcription PCR(RT-qPCR)and MeRIP-qPCR.GO and KEGG analysis indicated that differentially m^(6)A methylated genes(DMMGs)with significant differences in mRNA expression were closely related to processes such as regulation of gene expression,epigenetic,microtubule,autophagy-other and TOR signaling.Moreover,a total of 96 DMMGs without significant differences in mRNA expression showed significant differences at protein level.GO and pathway enrichment analysis of the 96 genes showed that RNA methylation may be involved in cell biosynthesis and metabolism of E.tenella.We firstly present a map of RNA m^(6)A modification in E.tenella,which provides significant insights into developmental biology of E.tenella. | Qing Liu Bingjin Mu Yijing Meng Linmei Yu Zirui Wang Tao Jia Wenbin Zheng Wenwei Gao Shichen Xie Xingquan Zhu | 2024 | Journal of Integrative Agriculture2024，23，1： | 0 |
| 2 | TaVNS reduces inflammatory responses in a L-NAME-induced rat model of pre-eclampsia显示文摘Pre-eclampsia is characterized by an excessive maternal inflammatory response.The cholinergic antiinflammatory pathway(CAP)has been shown as the efferent arm of a vagal reflex with the potential to limit inflammatory responses.Therefore,in this study,the CAP regulation through the nervous vagal stimulation(VNS)reduced the severity of NG-nitro-L-arginine methyl ester(L-NAME)-induced pre-eclampsia was determined in a rat model.Rats were given 125 mg/kg/day of L-NAME via subcutaneous injection on gestational day(GD)10–16.In addition,the rats were treated by active or sham electrical stimulation once a day during GD 13–19.Systolic blood pressure(SBP),urinary albumin,and pregnancy outcomes were documented for each rat.The average fetal weights and crown-rump length(CRL)as well as the placental weights of rats in both control and experimental groups were recorded onthe 13th day,16th day and 20th day of gestation.Subsequently,placentas were collected from the rats on GD20 to measure the level of cytokines.In addition,qRT-PCR and Western blot analysis were used to detect the mRNA and protein expression ofα7 nicotinic acetylcholine receptor(α7nAChR)and nuclear factor-κB(NF-κB),respectively.Immunohistochemistry assays were also carried out to determine the location and level ofα7nAChR and NF-κB in placentas.CAP regulation through the transcutaneous auricular nerve stimulation alleviated the clinical symptoms in the rats after L-NAME induction,including hypertension,proteinuria,fetal growth retardation and fetal death.In addition,TaVNS also increasedα7nAChR expression,reduced NF-κB p65 expression,and reversed LNAME-induced proinflammatory cytokines in the placenta tissues,including tumor necrosis factor-alpha(TNF-α),high mobility group box 1(HMGB-1)and interleukin-6(IL-6).The findings of this study showed that TaVNS might be used as a promising tool to attenuate pre-eclampsia-like symptoms.In addition,the protective effect of TaVNS was associated with the improvement ofα7nAChR expression and the inhibition of inflammatory reactions at the maternal-fetal interface through activating cholinergic anti-inflammation pathway. | LINMEI ZHENG RONG TANG LEI SHI ZHONGYI ZHOU | 2021 | BIOCELL2021，45，5： | 0 |
| 3 | Monolithic integrated micro-supercapacitors with ultra-high systemic volumetric performance and areal output voltage显示文摘Monolithic integrated micro-supercapacitors(MIMSCs) with high systemic performance and cell-number density are important for miniaturized electronics to empower the Internet of Things.However,fabrication of customizable MIMSCs in an extremely small space remains a huge challenge considering key factors such as materials selection,electrolyte confinement,microfabrication and device-performance uniformity.Here,we develop a universal and large-throughput microfabrication strategy to address all these issues by combining multistep lithographic patterning,spray printing of MXene micro electrodes and controllable 3D printing of gel electrolytes.We achieve the monolithic integration of electrochemically isolated micro-supercapacitors in close proximity by leveraging high-resolution micropatterning techniques for micro electrode deposition and 3D printing for precise electrolyte deposition.Notably,the MIMSCs obtained demonstrate a high areal-number density of 28 cells cm^(-2)(340 cells on 3.5 × 3.5 cm^(2)),a record areal output voltage of 75.6 V cm^(-2),an acceptable systemic volumetric energy density of 9.8 mWh cm^(-3)and an unprecedentedly high capacitance retention of 92% after 4000 cycles at an extremely high output voltage of 162 V.This work paves the way for monolithic integrated and microscopic energy-storage assemblies for powering future microele ctronics. | Sen Wang Linmei Li Shuanghao Zheng Pratteek Das Xiaoyu Shi Jiaxin Ma Yu Liu Yuanyuan Zhu Yao Lu Zhong-Shuai Wu Hui-Ming Cheng | 2023 | National Science Review2023，10，3： | 0 |
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